Probable xyloglucan endotransglucosylase/hydrolase protein 33 precursor

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Q8LC45 (XTH33_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 89. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 33
Short name=At-XTH33
Short name=XTH-33
EC=2.4.1.207

Gene names

Name:	XTH33
Ordered Locus Names:	At1g10550
ORF Names:	T10O24.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.
Catalytic activity	Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.
Subcellular location	Secreted › cell wall Probable. Secreted › extracellular space › apoplast Probable.
Tissue specificity	Predominantly expressed in green siliques. Ref.4
Post-translational modification	Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.
Miscellaneous	In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. XTH group 3 subfamily.
Sequence caution	The sequence AAM63851.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular glucan metabolic process Inferred from electronic annotation. Source: InterPro plant-type cell wall modification involved in multidimensional cell growth Inferred from mutant phenotype PubMed 19154201. Source: TAIR
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Inferred from direct assay PubMed 19154201. Source: TAIR
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro xyloglucan:xyloglucosyl transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

Potential

Chain

22 – 310

289

Probable xyloglucan endotransglucosylase/hydrolase protein 33

PRO_0000011833

Sites

Active site

118

Nucleophile By similarity

Active site

122

Proton donor By similarity

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

141

N-linked (GlcNAc...) Potential

Glycosylation

245

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

S → A in AAM63851. Ref.3

Sequence conflict

K → N in AAM63851. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q8LC45 [UniParc].

Last modified November 28, 2003. Version 2.
Checksum: F3228D8CB96EF89D
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FASTA

310

34,644

        10         20         30         40         50         60 
MKIMWETAVV FCLCSLSLVS SHSRKFTTPN VTRLTDQFSK IAIENGFSRR FGAHNIQVNG 

        70         80         90        100        110        120 
SLAKLTLDKS SGAGLVSKNK YHYGFFSARL KLPAGFASGV VVAFYLSNAE TYPKSHDEID 

       130        140        150        160        170        180 
IELLGRSRRD DWTIQTNVYA NGSTRTGREE KFYFWFDPTQ AFHDYTLIWN SHHTVFLVDN 

       190        200        210        220        230        240 
IPVRQFPNRG AFTSAYPSKP MSLYVTVWDG SEWATKGGKY PVNYKYAPFV VSVADVELSG 

       250        260        270        280        290        300 
CSVNNGSSTG SGPCTKSGGS ISSLDPVDGQ DFATLSKNQI NAMDWARRKL MFYSYCSDKP 

       310 
RYKVMPAECN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis." Yokoyama R., Nishitani K. Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature." Rose J.K.C., Braam J., Fry S.C., Nishitani K. Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Web resources

XTH-World

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007067 Genomic DNA. Translation: AAD39577.1. CP002684 Genomic DNA. Translation: AEE28593.1. AY086802 mRNA. Translation: AAM63851.1. Different initiation.
IPI	IPI00541660.
PIR	A86239.
RefSeq	NP_172525.1. NM_100930.3.
UniGene	At.42175.
3D structure databases
ProteinModelPortal	Q8LC45.
SMR	Q8LC45. Positions 60-309.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G10550.1-P.
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Proteomic databases
PRIDE	Q8LC45.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G10550.1; AT1G10550.1; AT1G10550.
GeneID	837596.
KEGG	ath:AT1G10550.
Organism-specific databases
TAIR	At1g10550.
Phylogenomic databases
eggNOG	COG2273.
HOGENOM	HOG000236368.
InParanoid	Q8LC45.
KO	K08235.
OMA	KLMFYSY.
PhylomeDB	Q8LC45.
ProtClustDB	CLSN2679589.
Gene expression databases
Genevestigator	Q8LC45.
GermOnline	AT1G10550. Arabidopsis thaliana.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR016455. XET. IPR010713. XET_C. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. PF06955. XET_C. 1 hit. [Graphical view]
PIRSF	PIRSF005604. XET. 1 hit.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XTH33_ARATH

Accession

Primary (citable) accession number: Q8LC45
Secondary accession number(s): Q9XIJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents