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Protein

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 1, mitochondrial

Gene

SDH2-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).By similarity

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster.By similarity
  • [3Fe-4S] clusterBy similarityNote: Binds 1 [3Fe-4S] cluster.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (eukaryal route).Curated
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate dehydrogenase [ubiquinone] flavoprotein subunit 1, mitochondrial (SDH1-1), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (AXX17_At5g38380), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (AXX17_At3g29870), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial (SDH2-3), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 1, mitochondrial (SDH2-1), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (AXX17_At2g13790), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 2, mitochondrial (SDH2-2), Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial (SDH1-2), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (AXX17_At5g66760)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (eukaryal route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi102Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi107Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi122Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi194Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi197Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi200Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi204Iron-sulfur 3 (3Fe-4S)By similarity1
Binding sitei209Ubiquinone; shared with SdhD subunitBy similarity1
Metal bindingi251Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi257Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi261Iron-sulfur 2 (4Fe-4S)By similarity1

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: GO_Central
  • 3 iron, 4 sulfur cluster binding Source: GO_Central
  • 4 iron, 4 sulfur cluster binding Source: GO_Central
  • electron carrier activity Source: TAIR
  • succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  • succinate dehydrogenase activity Source: TAIR
  • zinc ion binding Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BRENDAi1.3.5.1. 399.
UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 1, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Iron-sulfur subunit of complex II
Short name:
Ip
Gene namesi
Name:SDH2-1
Ordered Locus Names:At3g27380
ORF Names:K1G2.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G27380.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrion Source: TAIR
  • respiratory chain complex II Source: UniProtKB
  • succinate dehydrogenase complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28MitochondrionSequence analysisAdd BLAST28
ChainiPRO_000024759529 – 279Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 1, mitochondrialAdd BLAST251

Proteomic databases

PaxDbiQ8LBZ7.

Expressioni

Tissue specificityi

Ubiquitous. Preferentially expressed in flowers and inflorescences.2 Publications

Developmental stagei

Expressed in floral meristems and sex organ primordia at early stages of development. Later expressed in anthers, particularly in the tapetum, pollen mother cells, and microspores.1 Publication

Gene expression databases

GenevisibleiQ8LBZ7. AT.

Interactioni

Subunit structurei

Component of complex II composed of eight subunits in plants: four classical SDH subunits SDH1, SDH2, SDH3 and SDH4 (a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.), as well as four subunits unknown in mitochondria from bacteria and heterotrophic eukaryotes.1 Publication

Protein-protein interaction databases

IntActiQ8LBZ7. 2 interactors.
STRINGi3702.AT3G27380.1.

Structurei

3D structure databases

ProteinModelPortaliQ8LBZ7.
SMRiQ8LBZ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 1412Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST90
Domaini184 – 2144Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST31

Sequence similaritiesi

Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3049. Eukaryota.
COG0479. LUCA.
HOGENOMiHOG000160590.
InParanoidiQ8LBZ7.
KOiK00235.
OMAiLACKESM.
OrthoDBiEOG093609RV.
PhylomeDBiQ8LBZ7.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8LBZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGLIGRLV GTKPSKLATA ARLIPARWTS TGAEAETKAS SGGGRGSNLK
60 70 80 90 100
TFQIYRWNPD NPGKPELQNY QIDLKDCGPM VLDALIKIKN EMDPSLTFRR
110 120 130 140 150
SCREGICGSC AMNIDGCNGL ACLTKIQDEA SETTITPLPH MFVIKDLVVD
160 170 180 190 200
MTNFYNQYKS IEPWLKRKTP ASVPAKEILQ SKKDRAKLDG MYECILCACC
210 220 230 240 250
STSCPSYWWN PESYLGPAAL LHANRWISDS RDEYTKERLE AIDDEFKLYR
260 270
CHTILNCARA CPKGLNPGKQ ITHIKQLQR
Length:279
Mass (Da):31,171
Last modified:July 25, 2006 - v2
Checksum:iADA885EC59A3EA36
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20A → V in CAC19855 (PubMed:11442063).Curated1
Sequence conflicti20A → V in AAM63946 (Ref. 6) Curated1
Sequence conflicti26A → V in CAC19855 (PubMed:11442063).Curated1
Sequence conflicti26A → V in AAM63946 (Ref. 6) Curated1
Sequence conflicti200C → F in AAM63946 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278910 mRNA. Translation: CAC19855.1.
AB024028 Genomic DNA. Translation: BAA95713.1.
CP002686 Genomic DNA. Translation: AEE77309.1.
CP002686 Genomic DNA. Translation: AEE77310.1.
AY035013 mRNA. Translation: AAK59518.1.
AY063053 mRNA. Translation: AAL34227.1.
AY924777 mRNA. Translation: AAX23852.1.
AY086901 mRNA. Translation: AAM63946.1.
RefSeqiNP_001118718.1. NM_001125246.1.
NP_189374.1. NM_113653.4.
UniGeneiAt.17610.
At.22540.
At.32384.
At.71559.

Genome annotation databases

EnsemblPlantsiAT3G27380.1; AT3G27380.1; AT3G27380.
AT3G27380.2; AT3G27380.2; AT3G27380.
GeneIDi822359.
GrameneiAT3G27380.1; AT3G27380.1; AT3G27380.
AT3G27380.2; AT3G27380.2; AT3G27380.
KEGGiath:AT3G27380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278910 mRNA. Translation: CAC19855.1.
AB024028 Genomic DNA. Translation: BAA95713.1.
CP002686 Genomic DNA. Translation: AEE77309.1.
CP002686 Genomic DNA. Translation: AEE77310.1.
AY035013 mRNA. Translation: AAK59518.1.
AY063053 mRNA. Translation: AAL34227.1.
AY924777 mRNA. Translation: AAX23852.1.
AY086901 mRNA. Translation: AAM63946.1.
RefSeqiNP_001118718.1. NM_001125246.1.
NP_189374.1. NM_113653.4.
UniGeneiAt.17610.
At.22540.
At.32384.
At.71559.

3D structure databases

ProteinModelPortaliQ8LBZ7.
SMRiQ8LBZ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8LBZ7. 2 interactors.
STRINGi3702.AT3G27380.1.

Proteomic databases

PaxDbiQ8LBZ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G27380.1; AT3G27380.1; AT3G27380.
AT3G27380.2; AT3G27380.2; AT3G27380.
GeneIDi822359.
GrameneiAT3G27380.1; AT3G27380.1; AT3G27380.
AT3G27380.2; AT3G27380.2; AT3G27380.
KEGGiath:AT3G27380.

Organism-specific databases

TAIRiAT3G27380.

Phylogenomic databases

eggNOGiKOG3049. Eukaryota.
COG0479. LUCA.
HOGENOMiHOG000160590.
InParanoidiQ8LBZ7.
KOiK00235.
OMAiLACKESM.
OrthoDBiEOG093609RV.
PhylomeDBiQ8LBZ7.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.
BRENDAi1.3.5.1. 399.

Miscellaneous databases

PROiQ8LBZ7.

Gene expression databases

GenevisibleiQ8LBZ7. AT.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSDHB1_ARATH
AccessioniPrimary (citable) accession number: Q8LBZ7
Secondary accession number(s): Q9G3M0, Q9LTZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: November 30, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.