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Protein

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 1, mitochondrial

Gene

SDH2-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).By similarity

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster.By similarity
  • [3Fe-4S] clusterBy similarityNote: Binds 1 [3Fe-4S] cluster.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi102 – 1021Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi107 – 1071Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi122 – 1221Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi194 – 1941Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi197 – 1971Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi200 – 2001Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi204 – 2041Iron-sulfur 3 (3Fe-4S)By similarity
Binding sitei209 – 2091Ubiquinone; shared with SdhD subunitBy similarity
Metal bindingi251 – 2511Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi257 – 2571Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi261 – 2611Iron-sulfur 2 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  4. electron carrier activity Source: TAIR
  5. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  6. succinate dehydrogenase activity Source: TAIR
  7. zinc ion binding Source: TAIR

GO - Biological processi

  1. mitochondrial electron transport, succinate to ubiquinone Source: TAIR
  2. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciARA:GQT-2052-MONOMER.
UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 1, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Iron-sulfur subunit of complex II
Short name:
Ip
Gene namesi
Name:SDH2-1
Ordered Locus Names:At3g27380
ORF Names:K1G2.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G27380.

Subcellular locationi

Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Matrix side 1 Publication

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-SubCell
  2. mitochondrion Source: TAIR
  3. succinate dehydrogenase complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2828MitochondrionSequence AnalysisAdd
BLAST
Chaini29 – 279251Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 1, mitochondrialPRO_0000247595Add
BLAST

Proteomic databases

PaxDbiQ8LBZ7.
PRIDEiQ8LBZ7.

Expressioni

Tissue specificityi

Ubiquitous. Preferentially expressed in flowers and inflorescences.2 Publications

Developmental stagei

Expressed in floral meristems and sex organ primordia at early stages of development. Later expressed in anthers, particularly in the tapetum, pollen mother cells, and microspores.1 Publication

Gene expression databases

ExpressionAtlasiQ8LBZ7. baseline and differential.
GenevestigatoriQ8LBZ7.

Interactioni

Subunit structurei

Component of complex II composed of eight subunits in plants: four classical SDH subunits SDH1, SDH2, SDH3 and SDH4 (a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.), as well as four subunits unknown in mitochondria from bacteria and heterotrophic eukaryotes.1 Publication

Protein-protein interaction databases

IntActiQ8LBZ7. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8LBZ7.
SMRiQ8LBZ7. Positions 47-277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 141902Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini184 – 214314Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0479.
HOGENOMiHOG000160590.
InParanoidiQ8LBZ7.
KOiK00235.
OMAiDAAPFMQ.
PhylomeDBiQ8LBZ7.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8LBZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGLIGRLV GTKPSKLATA ARLIPARWTS TGAEAETKAS SGGGRGSNLK
60 70 80 90 100
TFQIYRWNPD NPGKPELQNY QIDLKDCGPM VLDALIKIKN EMDPSLTFRR
110 120 130 140 150
SCREGICGSC AMNIDGCNGL ACLTKIQDEA SETTITPLPH MFVIKDLVVD
160 170 180 190 200
MTNFYNQYKS IEPWLKRKTP ASVPAKEILQ SKKDRAKLDG MYECILCACC
210 220 230 240 250
STSCPSYWWN PESYLGPAAL LHANRWISDS RDEYTKERLE AIDDEFKLYR
260 270
CHTILNCARA CPKGLNPGKQ ITHIKQLQR
Length:279
Mass (Da):31,171
Last modified:July 25, 2006 - v2
Checksum:iADA885EC59A3EA36
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201A → V in CAC19855 (PubMed:11442063).Curated
Sequence conflicti20 – 201A → V in AAM63946 (Ref. 6) Curated
Sequence conflicti26 – 261A → V in CAC19855 (PubMed:11442063).Curated
Sequence conflicti26 – 261A → V in AAM63946 (Ref. 6) Curated
Sequence conflicti200 – 2001C → F in AAM63946 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278910 mRNA. Translation: CAC19855.1.
AB024028 Genomic DNA. Translation: BAA95713.1.
CP002686 Genomic DNA. Translation: AEE77309.1.
CP002686 Genomic DNA. Translation: AEE77310.1.
AY035013 mRNA. Translation: AAK59518.1.
AY063053 mRNA. Translation: AAL34227.1.
AY924777 mRNA. Translation: AAX23852.1.
AY086901 mRNA. Translation: AAM63946.1.
RefSeqiNP_001118718.1. NM_001125246.1.
NP_189374.1. NM_113653.3.
UniGeneiAt.17610.
At.22540.
At.32384.
At.71559.

Genome annotation databases

EnsemblPlantsiAT3G27380.1; AT3G27380.1; AT3G27380.
AT3G27380.2; AT3G27380.2; AT3G27380.
GeneIDi822359.
KEGGiath:AT3G27380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278910 mRNA. Translation: CAC19855.1.
AB024028 Genomic DNA. Translation: BAA95713.1.
CP002686 Genomic DNA. Translation: AEE77309.1.
CP002686 Genomic DNA. Translation: AEE77310.1.
AY035013 mRNA. Translation: AAK59518.1.
AY063053 mRNA. Translation: AAL34227.1.
AY924777 mRNA. Translation: AAX23852.1.
AY086901 mRNA. Translation: AAM63946.1.
RefSeqiNP_001118718.1. NM_001125246.1.
NP_189374.1. NM_113653.3.
UniGeneiAt.17610.
At.22540.
At.32384.
At.71559.

3D structure databases

ProteinModelPortaliQ8LBZ7.
SMRiQ8LBZ7. Positions 47-277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8LBZ7. 2 interactions.

Proteomic databases

PaxDbiQ8LBZ7.
PRIDEiQ8LBZ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G27380.1; AT3G27380.1; AT3G27380.
AT3G27380.2; AT3G27380.2; AT3G27380.
GeneIDi822359.
KEGGiath:AT3G27380.

Organism-specific databases

GeneFarmi2168. 175.
TAIRiAT3G27380.

Phylogenomic databases

eggNOGiCOG0479.
HOGENOMiHOG000160590.
InParanoidiQ8LBZ7.
KOiK00235.
OMAiDAAPFMQ.
PhylomeDBiQ8LBZ7.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.
BioCyciARA:GQT-2052-MONOMER.

Gene expression databases

ExpressionAtlasiQ8LBZ7. baseline and differential.
GenevestigatoriQ8LBZ7.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Three different genes encode the iron-sulphur subunit of succinate dehydrogenase in Arabidopsis thaliana."
    Figueroa P., Leon G., Elorza A., Holuigue L., Jordana X.
    Plant Mol. Biol. 46:241-250(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.
  8. "New insights into the respiratory chain of plant mitochondria. Supercomplexes and a unique composition of complex II."
    Eubel H., Jansch L., Braun H.P.
    Plant Physiol. 133:274-286(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Mitochondrial cytochrome c oxidase and succinate dehydrogenase complexes contain plant specific subunits."
    Millar A.H., Eubel H., Jansch L., Kruft V., Heazlewood J.L., Braun H.P.
    Plant Mol. Biol. 56:77-90(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
  10. "Nuclear SDH2-1 and SDH2-2 genes, encoding the iron-sulfur subunit of mitochondrial complex II in Arabidopsis, have distinct cell-specific expression patterns and promoter activities."
    Elorza A., Leon G., Gomez I., Mouras A., Holuigue L., Araya A., Jordana X.
    Plant Physiol. 136:4072-4087(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSDHB1_ARATH
AccessioniPrimary (citable) accession number: Q8LBZ7
Secondary accession number(s): Q9G3M0, Q9LTZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: February 4, 2015
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.