Q8LBL5 (PRT1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase PRT1
EC=6.3.2.-

Alternative name(s):
Proteolysis 1 protein

Gene names

Name:	PRT1
Ordered Locus Names:	At3g24800
ORF Names:	K7P8.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. Functions in the N-end rule pathway of protein degradation, where it specifically recognizes and ubiquitinates proteins with a N-terminal bulky aromatic amino acid. Does not act on aliphatic hydrophobic and basic N-terminal residues containing proteins. Ref.1 Ref.6 Ref.7
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Contains 2 RING-type zinc fingers. Contains 1 ZZ-type zinc finger.
Sequence caution	The sequence AAM64697.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	E3 ubiquitin-protein ligase PRT1		PRO_0000056003
Regions
	Zinc finger	26 – 66	41	RING-type 1
	Zinc finger	192 – 232	41	RING-type 2
	Zinc finger	305 – 355	51	ZZ-type
Experimental info
	Sequence conflict	394	1	E → D in AAM64697. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q8LBL5 [UniParc]. Last modified April 26, 2005. Version 2. Checksum: 17463F96CB6DBF54	FASTA	410	45,937
	10 20 30 40 50 60 MAETMKDITM KNDESQEEEI PDQFLCCVCL ELLYKPIVLS CGHLSCFWCV HKSMNGFRES 70 80 90 100 110 120 HCPICRDPYV HFPSVCQKLY FLLKKMYPLA HKKREEQVLK EEQERECFSP QIDLVLDLSV 130 140 150 160 170 180 CSGDSLNVSD KQKVEECSNA ANLLSSSSSR GDIPCIPKNQ EPTDAKALNV HENELLKDNK 190 200 210 220 230 240 VSKQISKDDL LCSACKELLV RPVVLNCGHV YCEGCVVDMA EESEKIKCQE CNVCDPRGFP 250 260 270 280 290 300 KVCLILEQLL EENFPEEYNS RSSKVQKTLA HNSKGNIQSY LKEGPSLSND NNNDDPWLAN 310 320 330 340 350 360 PGSNVHFGAG CDSCGVYPII GDRYRCKDCK EEIGYDLCKD CYETPSKVPG RFNQQHTPDH 370 380 390 400 410 RLELARSPQV LINFNSIGIL LGPVISNEGM DTDEGEEGPP GSSNESSSTE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PRT1 of Arabidopsis thaliana encodes a component of the plant N-end rule pathway." Potuschak T., Stary S., Schloegelhofer P., Becker F., Nejinskaia V., Bachmair A. Proc. Natl. Acad. Sci. U.S.A. 95:7904-7908(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Use of a reporter transgene to generate Arabidopsis mutants in ubiquitin-dependent protein degradation." Bachmair A., Becker F., Schell J. Proc. Natl. Acad. Sci. U.S.A. 90:418-421(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTANTS PRT1.
[7]	"PRT1 of Arabidopsis is a ubiquitin protein ligase of the plant N-end rule pathway with specificity for aromatic amino-terminal residues." Stary S., Yin X.-J., Potuschak T., Schloegelhofer P., Nizhynska V., Bachmair A. Plant Physiol. 133:1360-1366(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ224306 Genomic DNA. Translation: CAA11891.1. AJ224307 mRNA. Translation: CAA11892.1. AB028609 Genomic DNA. Translation: BAB02890.1. CP002686 Genomic DNA. Translation: AEE76948.1. AY080799 mRNA. Translation: AAL87280.1. AY114077 mRNA. Translation: AAM45125.1. AY087139 mRNA. Translation: AAM64697.1. Different initiation.
IPI	IPI00532279.
PIR	T52193.
RefSeq	NP_189124.1. NM_113392.2.
UniGene	At.77.
3D structure databases
ProteinModelPortal	Q8LBL5.
SMR	Q8LBL5. Positions 15-84, 187-252.
ModBase	Search...
Proteomic databases
PaxDb	Q8LBL5.
PRIDE	Q8LBL5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G24800.1; AT3G24800.1; AT3G24800.
GeneID	822078.
KEGG	ath:AT3G24800.
Organism-specific databases
GeneFarm	4910.
TAIR	At3g24800.
Phylogenomic databases
eggNOG	NOG251630.
HOGENOM	HOG000030197.
InParanoid	Q8LBL5.
OMA	VCLELLY.
PhylomeDB	Q8LBL5.
ProtClustDB	CLSN2915347.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q8LBL5.
GermOnline	AT3G24800. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.30.40.10. 3 hits.
InterPro	IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. IPR000433. Znf_ZZ. [Graphical view]
Pfam	PF00569. ZZ. 1 hit. [Graphical view]
SMART	SM00184. RING. 2 hits. SM00291. ZnF_ZZ. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 2 hits. PS01357. ZF_ZZ_1. 1 hit. PS50135. ZF_ZZ_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRT1_ARATH

Accession

Primary (citable) accession number: Q8LBL5
Secondary accession number(s): O82767

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	May 1, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program