Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 2, mitochondrial precursor

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Q8LB02 (DHSB2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 91. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 2, mitochondrial
EC=1.3.5.1

Alternative name(s):
Iron-sulfur subunit of complex II
Short name=Ip

Gene names

Name:	SDH2-2
Ordered Locus Names:	At5g40650
ORF Names:	MNF13.170, MNF13.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
Subunit structure	Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b composed of a large and a small subunit By similarity. Ref.6
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Tissue specificity	Ubiquitous. Preferentially expressed in flowers, inflorescences and root tips. Ref.1 Ref.6 Ref.8
Developmental stage	Expressed in floral meristems and sex organ primordia at early stages of development. Later expressed in anthers, particularly in the tapetum, pollen mother cells, and microspores. Ref.8
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial electron transport, succinate to ubiquinone Inferred from sequence or structural similarity Ref.1. Source: TAIR tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	mitochondrial respiratory chain complex II Inferred from direct assay PubMed 15604729. Source: TAIR
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from sequence or structural similarity Ref.1. Source: TAIR succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC succinate dehydrogenase activity Inferred from sequence or structural similarity Ref.1. Source: TAIR zinc ion binding Inferred from direct assay PubMed 20018591. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 28

Mitochondrion Potential

Chain

29 – 280

252

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 2, mitochondrial

PRO_0000247596

Regions

Domain

51 – 140

2Fe-2S ferredoxin-type

Domain

183 – 213

4Fe-4S ferredoxin-type

Sites

Metal binding

101

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

106

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

109

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

121

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

193

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

196

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

199

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

203

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

250

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

256

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

260

Iron-sulfur 2 (4Fe-4S) By similarity

Binding site

208

Ubiquinone; shared with DHSD By similarity

Experimental info

Sequence conflict

S → P in CAC19856. Ref.1

Sequence conflict

S → P in AAM65047. Ref.5

Sequence conflict

T → K in CAC19856. Ref.1

Sequence conflict

T → K in AAM65047. Ref.5

Sequence conflict

127

S → F in CAC19856. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q8LB02 [UniParc].

Last modified July 25, 2006. Version 2.
Checksum: 1F505A8C7D68DACD
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FASTA

280

31,141

        10         20         30         40         50         60 
MAFGLIGRVV GTKSSRLSTA ARLIPARWTS TGSEAQSKAS TGGGGASLKT FQIYRWNPDN 

        70         80         90        100        110        120 
PGKPELQDYK IDLKDCGPMV LDALIKIKNE MDPSLTFRRS CREGICGSCA MNIDGCNGLA 

       130        140        150        160        170        180 
CLTKIESGSK ETTITPLPHM FVIKDLVVDM TNFYNQYKSI EPWLKRKNPA SVPGKEILQS 

       190        200        210        220        230        240 
KKDRAKLDGM YECILCACCS TSCPSYWWNP ESYLGPAALL HANRWISDSR DEYTKERLEA 

       250        260        270        280 
IDDEFKLYRC HTILNCARAC PKGLNPGKQI THIKQLQKSG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three different genes encode the iron-sulphur subunit of succinate dehydrogenase in Arabidopsis thaliana." Figueroa P., Leon G., Elorza A., Holuigue L., Jordana X. Plant Mol. Biol. 46:241-250(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The four subunits of mitochondrial respiratory complex II are encoded by multiple nuclear genes and targeted to mitochondria in Arabidopsis thaliana." Figueroa P., Leon G., Elorza A., Holuigue L., Araya A., Jordana X. Plant Mol. Biol. 50:725-734(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, TISSUE SPECIFICITY.
[7]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Strain: cv. Landsberg erecta.
[8]	"Nuclear SDH2-1 and SDH2-2 genes, encoding the iron-sulfur subunit of mitochondrial complex II in Arabidopsis, have distinct cell-specific expression patterns and promoter activities." Elorza A., Leon G., Gomez I., Mouras A., Holuigue L., Araya A., Jordana X. Plant Physiol. 136:4072-4087(2004) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ278911 mRNA. Translation: CAC19856.1. AB009052 Genomic DNA. Translation: BAB08537.1. CP002688 Genomic DNA. Translation: AED94578.1. AY074299 mRNA. Translation: AAL66996.1. AY123036 mRNA. Translation: AAM67569.1. AY087504 mRNA. Translation: AAM65047.1.
IPI	IPI00523799.
RefSeq	NP_198881.1. NM_123430.1.
UniGene	At.20145. At.67187. At.7421.
3D structure databases
ProteinModelPortal	Q8LB02.
SMR	Q8LB02. Positions 46-279.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8LB02. 1 interaction.
Proteomic databases
PaxDb	Q8LB02.
PRIDE	Q8LB02.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G40650.1; AT5G40650.1; AT5G40650.
GeneID	834065.
KEGG	ath:AT5G40650.
Organism-specific databases
GeneFarm	2169. 175.
TAIR	At5g40650.
Phylogenomic databases
eggNOG	COG0479.
HOGENOM	HOG000160590.
InParanoid	Q8LB02.
KO	K00235.
OMA	NCSRTCP.
PhylomeDB	Q8LB02.
ProtClustDB	PLN00129.
Enzyme and pathway databases
BioCyc	MetaCyc:AT5G40650-MONOMER.
UniPathway	UPA00223; UER01006.
Gene expression databases
Genevestigator	Q8LB02.
GermOnline	AT5G40650. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.10.1060.10. 1 hit. 3.10.20.30. 1 hit.
InterPro	IPR001041. 2Fe-2S_ferredoxin-type. IPR006058. 2Fe2S_fd_BS. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012675. Beta-grasp_dom. IPR012285. Fum_reductase_C. IPR009051. Helical_ferredxn. IPR004489. Succ_DH/fum_Rdtase_Fe-S. IPR025192. Succ_DH/fum_Rdtase_N. [Graphical view]
Pfam	PF13085. Fer2_3. 1 hit. [Graphical view]
SUPFAM	SSF54292. Ferredoxin. 1 hit. SSF46548. Helical_ferredxn. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSB2_ARATH

Accession

Primary (citable) accession number: Q8LB02
Secondary accession number(s): Q9FM32, Q9G3L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	May 29, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents