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Q8LB02

- SDHB2_ARATH

UniProt

Q8LB02 - SDHB2_ARATH

Protein

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 2, mitochondrial

Gene

SDH2-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).By similarity

    Catalytic activityi

    Succinate + a quinone = fumarate + a quinol.

    Cofactori

    Binds 1 2Fe-2S cluster.By similarity
    Binds 1 3Fe-4S cluster.By similarity
    Binds 1 4Fe-4S cluster.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi101 – 1011Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi106 – 1061Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi109 – 1091Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi121 – 1211Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi193 – 1931Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi196 – 1961Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi199 – 1991Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi203 – 2031Iron-sulfur 3 (3Fe-4S)By similarity
    Binding sitei208 – 2081Ubiquinone; shared with DHSDBy similarity
    Metal bindingi250 – 2501Iron-sulfur 3 (3Fe-4S)By similarity
    Metal bindingi256 – 2561Iron-sulfur 3 (3Fe-4S)By similarity
    Metal bindingi260 – 2601Iron-sulfur 2 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    4. electron carrier activity Source: TAIR
    5. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
    6. succinate dehydrogenase activity Source: TAIR
    7. zinc ion binding Source: TAIR

    GO - Biological processi

    1. mitochondrial electron transport, succinate to ubiquinone Source: TAIR
    2. tricarboxylic acid cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport, Tricarboxylic acid cycle

    Keywords - Ligandi

    2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:AT5G40650-MONOMER.
    UniPathwayiUPA00223; UER01006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 2, mitochondrial (EC:1.3.5.1)
    Alternative name(s):
    Iron-sulfur subunit of complex II
    Short name:
    Ip
    Gene namesi
    Name:SDH2-2
    Ordered Locus Names:At5g40650
    ORF Names:MNF13.170, MNF13.21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G40650.

    Subcellular locationi

    Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Matrix side 1 Publication

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex II Source: TAIR
    2. mitochondrion Source: TAIR
    3. succinate dehydrogenase complex Source: TAIR

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2828MitochondrionSequence AnalysisAdd
    BLAST
    Chaini29 – 280252Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 2, mitochondrialPRO_0000247596Add
    BLAST

    Proteomic databases

    PaxDbiQ8LB02.
    PRIDEiQ8LB02.

    Expressioni

    Tissue specificityi

    Ubiquitous. Preferentially expressed in flowers, inflorescences and root tips.3 Publications

    Developmental stagei

    Expressed in floral meristems and sex organ primordia at early stages of development. Later expressed in anthers, particularly in the tapetum, pollen mother cells, and microspores.1 Publication

    Gene expression databases

    GenevestigatoriQ8LB02.

    Interactioni

    Subunit structurei

    Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b composed of a large and a small subunit.By similarity

    Protein-protein interaction databases

    IntActiQ8LB02. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LB02.
    SMRiQ8LB02. Positions 46-279.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini51 – 140902Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini183 – 213314Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0479.
    HOGENOMiHOG000160590.
    InParanoidiQ8LB02.
    KOiK00235.
    OMAiIMNCSRT.
    PhylomeDBiQ8LB02.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR006058. 2Fe2S_fd_BS.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR012675. Beta-grasp_dom.
    IPR009051. Helical_ferredxn.
    IPR004489. Succ_DH/fum_Rdtase_Fe-S.
    IPR025192. Succ_DH/fum_Rdtase_N.
    [Graphical view]
    PfamiPF13085. Fer2_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF46548. SSF46548. 1 hit.
    SSF54292. SSF54292. 1 hit.
    TIGRFAMsiTIGR00384. dhsB. 1 hit.
    PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
    PS51085. 2FE2S_FER_2. 1 hit.
    PS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8LB02-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFGLIGRVV GTKSSRLSTA ARLIPARWTS TGSEAQSKAS TGGGGASLKT    50
    FQIYRWNPDN PGKPELQDYK IDLKDCGPMV LDALIKIKNE MDPSLTFRRS 100
    CREGICGSCA MNIDGCNGLA CLTKIESGSK ETTITPLPHM FVIKDLVVDM 150
    TNFYNQYKSI EPWLKRKNPA SVPGKEILQS KKDRAKLDGM YECILCACCS 200
    TSCPSYWWNP ESYLGPAALL HANRWISDSR DEYTKERLEA IDDEFKLYRC 250
    HTILNCARAC PKGLNPGKQI THIKQLQKSG 280
    Length:280
    Mass (Da):31,141
    Last modified:July 25, 2006 - v2
    Checksum:i1F505A8C7D68DACD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141S → P in CAC19856. (PubMed:11442063)Curated
    Sequence conflicti14 – 141S → P in AAM65047. 1 PublicationCurated
    Sequence conflicti31 – 311T → K in CAC19856. (PubMed:11442063)Curated
    Sequence conflicti31 – 311T → K in AAM65047. 1 PublicationCurated
    Sequence conflicti127 – 1271S → F in CAC19856. (PubMed:11442063)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ278911 mRNA. Translation: CAC19856.1.
    AB009052 Genomic DNA. Translation: BAB08537.1.
    CP002688 Genomic DNA. Translation: AED94578.1.
    AY074299 mRNA. Translation: AAL66996.1.
    AY123036 mRNA. Translation: AAM67569.1.
    AY087504 mRNA. Translation: AAM65047.1.
    RefSeqiNP_198881.1. NM_123430.1.
    UniGeneiAt.20145.
    At.67187.
    At.7421.

    Genome annotation databases

    EnsemblPlantsiAT5G40650.1; AT5G40650.1; AT5G40650.
    GeneIDi834065.
    KEGGiath:AT5G40650.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ278911 mRNA. Translation: CAC19856.1 .
    AB009052 Genomic DNA. Translation: BAB08537.1 .
    CP002688 Genomic DNA. Translation: AED94578.1 .
    AY074299 mRNA. Translation: AAL66996.1 .
    AY123036 mRNA. Translation: AAM67569.1 .
    AY087504 mRNA. Translation: AAM65047.1 .
    RefSeqi NP_198881.1. NM_123430.1.
    UniGenei At.20145.
    At.67187.
    At.7421.

    3D structure databases

    ProteinModelPortali Q8LB02.
    SMRi Q8LB02. Positions 46-279.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8LB02. 1 interaction.

    Proteomic databases

    PaxDbi Q8LB02.
    PRIDEi Q8LB02.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G40650.1 ; AT5G40650.1 ; AT5G40650 .
    GeneIDi 834065.
    KEGGi ath:AT5G40650.

    Organism-specific databases

    GeneFarmi 2169. 175.
    TAIRi AT5G40650.

    Phylogenomic databases

    eggNOGi COG0479.
    HOGENOMi HOG000160590.
    InParanoidi Q8LB02.
    KOi K00235.
    OMAi IMNCSRT.
    PhylomeDBi Q8LB02.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER01006 .
    BioCyci MetaCyc:AT5G40650-MONOMER.

    Gene expression databases

    Genevestigatori Q8LB02.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR001041. 2Fe-2S_ferredoxin-type.
    IPR006058. 2Fe2S_fd_BS.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR012675. Beta-grasp_dom.
    IPR009051. Helical_ferredxn.
    IPR004489. Succ_DH/fum_Rdtase_Fe-S.
    IPR025192. Succ_DH/fum_Rdtase_N.
    [Graphical view ]
    Pfami PF13085. Fer2_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46548. SSF46548. 1 hit.
    SSF54292. SSF54292. 1 hit.
    TIGRFAMsi TIGR00384. dhsB. 1 hit.
    PROSITEi PS00197. 2FE2S_FER_1. 1 hit.
    PS51085. 2FE2S_FER_2. 1 hit.
    PS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Three different genes encode the iron-sulphur subunit of succinate dehydrogenase in Arabidopsis thaliana."
      Figueroa P., Leon G., Elorza A., Holuigue L., Jordana X.
      Plant Mol. Biol. 46:241-250(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
      Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "The four subunits of mitochondrial respiratory complex II are encoded by multiple nuclear genes and targeted to mitochondria in Arabidopsis thaliana."
      Figueroa P., Leon G., Elorza A., Holuigue L., Araya A., Jordana X.
      Plant Mol. Biol. 50:725-734(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, TISSUE SPECIFICITY.
    7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.
    8. "Nuclear SDH2-1 and SDH2-2 genes, encoding the iron-sulfur subunit of mitochondrial complex II in Arabidopsis, have distinct cell-specific expression patterns and promoter activities."
      Elorza A., Leon G., Gomez I., Mouras A., Holuigue L., Araya A., Jordana X.
      Plant Physiol. 136:4072-4087(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiSDHB2_ARATH
    AccessioniPrimary (citable) accession number: Q8LB02
    Secondary accession number(s): Q9FM32, Q9G3L9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3