ID   DAPB2_ARATH             Reviewed;         349 AA.
AC   Q8LB01; Q3EAH1; Q9M1Y5;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 37.
DE   RecName: Full=Dihydrodipicolinate reductase 2, chloroplastic;
DE            Short=DHPR 2;
DE            EC=1.3.1.26;
DE   Flags: Precursor;
GN   Name=DAPB2; OrderedLocusNames=At3g59890; ORFNames=F24G16.160;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016720; PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M.,
RA   Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B.,
RA   Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P.,
RA   De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P.,
RA   Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V.,
RA   Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S.,
RA   Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G.,
RA   Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B.,
RA   Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G.,
RA   Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J.,
RA   Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D.,
RA   Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D.,
RA   Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W.,
RA   Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J.,
RA   Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P.,
RA   Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S.,
RA   Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V.,
RA   Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C.,
RA   Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E.,
RA   Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y.,
RA   Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A.,
RA   Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=cv. Columbia;
RA   Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION.
RX   PubMed=15652176; DOI=10.1016/j.bbagen.2004.09.008;
RA   Hudson A.O., Bless C., Macedo P., Chatterjee S.P., Singh B.K.,
RA   Gilvarg C., Leustek T.;
RT   "Biosynthesis of lysine in plants: evidence for a variant of the known
RT   bacterial pathways.";
RL   Biochim. Biophys. Acta 1721:27-36(2005).
CC   -!- CATALYTIC ACTIVITY: 2,3,4,5-tetrahydrodipicolinate + NAD(P)(+) =
CC       2,3-dihydrodipicolinate + NAD(P)H.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP
CC       pathway; tetrahydrodipicolinate from L-aspartate: step 4/4.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast (Potential).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q8LB01-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8LB01-2; Sequence=VSP_028629;
CC         Note=Derived from EST data. No experimental confirmation
CC         available;
CC   -!- SIMILARITY: Belongs to the dihydrodipicolinate reductase family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB75808.1; Type=Erroneous gene model prediction;
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AL138647; CAB75808.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AY087505; AAM65048.1; -; mRNA.
DR   EMBL; BT026511; ABH04618.1; -; mRNA.
DR   IPI; IPI00521803; -.
DR   IPI; IPI00523614; -.
DR   PIR; T47813; T47813.
DR   RefSeq; NP_567088.1; -.
DR   RefSeq; NP_974464.1; -.
DR   UniGene; At.34540; -.
DR   PRIDE; Q8LB01; -.
DR   GeneID; 825159; -.
DR   GenomeReviews; BA000014_GR; AT3G59890.
DR   KEGG; ath:AT3G59890; -.
DR   TAIR; At3g59890; -.
DR   OMA; Q8LB01; VDYTVPD.
DR   BRENDA; 1.3.1.26; 302.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0008839; F:dihydrodipicolinate reductase activity; IGI:TAIR.
DR   GO; GO:0019877; P:diaminopimelate biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000846; DapB.
DR   InterPro; IPR011770; DapB_bac/pln.
DR   InterPro; IPR011859; Dihydrodipicolinate_Rdtase_pln.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR20836; DapB_bac/pln; 1.
DR   Pfam; PF01113; DapB_N; 1.
DR   ProDom; PD004105; DapB; 1.
DR   TIGRFAMs; TIGR02130; dapB_plant; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Amino-acid biosynthesis; Chloroplast;
KW   Complete proteome; Diaminopimelate biosynthesis; Lysine biosynthesis;
KW   NADP; Oxidoreductase; Plastid; Transit peptide.
FT   TRANSIT       1     53       Chloroplast (Potential).
FT   CHAIN        54    349       Dihydrodipicolinate reductase 2,
FT                                chloroplastic.
FT                                /FTId=PRO_0000307183.
FT   VAR_SEQ       1      6       Missing (in isoform 2).
FT                                /FTId=VSP_028629.
SQ   SEQUENCE   349 AA;  37870 MW;  D7CA92482ECA28CA CRC64;
     MAANGLMAAS SVFLHRPVHP HFSFSSRTNQ MVPLGFKGRV SFIGNVKRCF PVVLSMGKSE
     TFEEAGNSVA PGNGISIMVN GCSGKMGKAV IKAADSAGVN IVPTSFGSVE EAGQTVEVCG
     KEILVHGPTE REKVLSSVFE KYPELIVVDY TIPSAVNDNA ELYGKVGVPF VMGTTGGDRT
     RLYKTVEESK IYAVISPQMG KQVVAFLAAM EIMSEQFPGA FAGYSLEVME SHQASKLDAS
     GTAKAVISCF QKLGVSYDMD QIQLIRDPKQ QIEVVGVPEE HVSGHAFHLY HLTSPDKTVS
     FEFQHNVCGR SIYAEGTVDA VLFLAKKIRS KAEKRIYNMI DVLREGNMR
//