Reviewed, UniProtKB/Swiss-Prot Q8LB01 (DAPB2_ARATH)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate reductase 2, chloroplastic
      Short name=DHPR 2
    EC=1.3.1.26

Gene names

Name:	DAPB2
Ordered Locus Names:	At3g59890
ORF Names:	F24G16.160

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	2,3,4,5-tetrahydrodipicolinate + NAD(P)+ = 2,3-dihydrodipicolinate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 4/4.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the dihydrodipicolinate reductase family.
Sequence caution	The sequence CAB75808.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro dihydrodipicolinate reductase activity Ref.4 Inferred from genetic interaction. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8LB01-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8LB01-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 53	53	Chloroplast Potential
	Chain	54 – 349	296	Dihydrodipicolinate reductase 2, chloroplastic		PRO_0000307183
Natural variations
	Alternative sequence	1 – 6	6	Missing in isoform 2.		VSP_028629

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D7CA92482ECA28CA	FASTA	349	37,870
	10 20 30 40 50 60 MAANGLMAAS SVFLHRPVHP HFSFSSRTNQ MVPLGFKGRV SFIGNVKRCF PVVLSMGKSE 70 80 90 100 110 120 TFEEAGNSVA PGNGISIMVN GCSGKMGKAV IKAADSAGVN IVPTSFGSVE EAGQTVEVCG 130 140 150 160 170 180 KEILVHGPTE REKVLSSVFE KYPELIVVDY TIPSAVNDNA ELYGKVGVPF VMGTTGGDRT 190 200 210 220 230 240 RLYKTVEESK IYAVISPQMG KQVVAFLAAM EIMSEQFPGA FAGYSLEVME SHQASKLDAS 250 260 270 280 290 300 GTAKAVISCF QKLGVSYDMD QIQLIRDPKQ QIEVVGVPEE HVSGHAFHLY HLTSPDKTVS 310 320 330 340 FEFQHNVCGR SIYAEGTVDA VLFLAKKIRS KAEKRIYNMI DVLREGNMR
	Isoform 2. Checksum: 404781E76BE7A86F	FASTA	343	37,312
	10 20 30 40 50 60 MAASSVFLHR PVHPHFSFSS RTNQMVPLGF KGRVSFIGNV KRCFPVVLSM GKSETFEEAG 70 80 90 100 110 120 NSVAPGNGIS IMVNGCSGKM GKAVIKAADS AGVNIVPTSF GSVEEAGQTV EVCGKEILVH 130 140 150 160 170 180 GPTEREKVLS SVFEKYPELI VVDYTIPSAV NDNAELYGKV GVPFVMGTTG GDRTRLYKTV 190 200 210 220 230 240 EESKIYAVIS PQMGKQVVAF LAAMEIMSEQ FPGAFAGYSL EVMESHQASK LDASGTAKAV 250 260 270 280 290 300 ISCFQKLGVS YDMDQIQLIR DPKQQIEVVG VPEEHVSGHA FHLYHLTSPD KTVSFEFQHN 310 320 330 340 VCGRSIYAEG TVDAVLFLAK KIRSKAEKRI YNMIDVLREG NMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Arabidopsis ORF clones." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Biosynthesis of lysine in plants: evidence for a variant of the known bacterial pathways." Hudson A.O., Bless C., Macedo P., Chatterjee S.P., Singh B.K., Gilvarg C., Leustek T. Biochim. Biophys. Acta 1721:27-36(2005) [PubMed: 15652176] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL138647 Genomic DNA. Translation: CAB75808.1. Sequence problems. AY087505 mRNA. Translation: AAM65048.1. BT026511 mRNA. Translation: ABH04618.1.
IPI	IPI00521803. IPI00523614.
PIR	T47813.
RefSeq	NP_567088.1. NP_974464.1.
UniGene	At.34540
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q8LB01.
Genome annotation databases
GeneID	825159.
GenomeReviews	Gene locus AT3G59890 in contig BA000014_GR.
KEGG	ath:AT3G59890.
Organism-specific databases
TAIR	At3g59890.
Phylogenomic databases
OMA	Q8LB01. VDYTVPD.
Enzyme and pathway databases
BRENDA	1.3.1.26. 302.
Family and domain databases
InterPro	IPR000846. DapB. IPR011770. DapB_bac/pln. IPR011859. Dihydrodipicolinate_Rdtase_pln. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR20836. DapB_bac/pln. 1 hit.
Pfam	PF01113. DapB_N. 1 hit. [Graphical view]
ProDom	PD004105. DapB. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02130. dapB_plant. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DAPB2_ARATH

Accession

Primary (citable) accession number: Q8LB01
Secondary accession number(s): Q3EAH1, Q9M1Y5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)