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Protein

COP9 signalosome complex subunit 5a

Gene

CSN5A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex (By similarity). The CSN complex is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of PSIAA6 by regulating the activity of the Ubl ligase SCF-TIR complex. Involved in CSN's deneddylation/derubylation activity (PubMed:15486099). Required for the deneddylation of all cullins (PubMed:15923347, PubMed:17307927). Essential for the structural integrity of the CSN holocomplex (PubMed:17307927).By similarity5 Publications

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi142Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi144Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi155Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • COP9 signalosome assembly Source: TAIR
  • negative regulation of photomorphogenesis Source: TAIR
  • positive regulation of G2/M transition of mitotic cell cycle Source: TAIR
  • protein deneddylation Source: TAIR
  • red, far-red light phototransduction Source: UniProtKB-KW
  • regulation of defense response Source: TAIR
  • response to auxin Source: TAIR
  • specification of floral organ identity Source: TAIR

Keywordsi

Molecular functionDevelopmental protein, Hydrolase, Metalloprotease, Protease
Biological processPhytochrome signaling pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-5696394. DNA Damage Recognition in GG-NER.
R-ATH-6781823. Formation of TC-NER Pre-Incision Complex.
R-ATH-8951664. Neddylation.

Protein family/group databases

MEROPSiM67.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
COP9 signalosome complex subunit 5a (EC:3.4.-.-)
Short name:
Signalosome subunit 5a
Alternative name(s):
Jun activation domain-binding homolog 1
Gene namesi
Name:CSN5A1 Publication
Synonyms:AJH11 Publication, CSN5B1 Publication
Ordered Locus Names:At1g22920Imported
ORF Names:F19G10.12Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G22920.
TAIRilocus:2017764. AT1G22920.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signalosome

Pathology & Biotechi

Disruption phenotypei

Short hypocotyl and open cotyledons in dark-grown seedling, accumulation of anthocyanin and expression of light-induced genes in the dark resulting from an impaired cullin deneddylation (PubMed:15923347). Severe developmental defects resulting in dwarf stature and loss of apical dominance (PubMed:17307927). Csn5a and csn5b double mutants are lethal at the seedling stage (PubMed:17307927).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi142H → A: No effect on CSN complex integrity but impaired CUL1 derubylation. 1 Publication1
Mutagenesisi144H → A: No effect on CSN complex integrity but impaired CUL1 derubylation. 1 Publication1
Mutagenesisi149C → A: No effect on CSN complex integrity and no effect on CUL1 derubylation. 1 Publication1
Mutagenesisi149C → S: No effect on CSN complex integrity but impaired CUL1 derubylation. 1 Publication1
Mutagenesisi155D → N: No effect on CSN complex integrity but impaired CUL1 derubylation. 1 Publication1
Mutagenesisi175D → E or N: No effect on CSN complex integrity but impaired CUL1 derubylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948441 – 357COP9 signalosome complex subunit 5aAdd BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ8LAZ7.
PRIDEiQ8LAZ7.

PTM databases

iPTMnetiQ8LAZ7.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highly expressed in flowers and roots. Expressed at lower level in seedlings and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ8LAZ7. baseline and differential.
GenevisibleiQ8LAZ7. AT.

Interactioni

Subunit structurei

Component of the CSN complex, probably composed of CSN1, CSN2, CSN3, CSN4, CSN5 (CSN5A or CSN5B), CSN6 (CSN6A or CSN6B), CSN7 and CSN8 (PubMed:9811788, PubMed:12615944, PubMed:15486099). CSN5A or CSN5B are present within distinct CSN complexes each containing only one copy of CSN5 (PubMed:15486099). Interacts with itself (PubMed:9811788). In the complex, it is located in the center and probably interacts directly with CSN4 and CSN6A or CSN6B (PubMed:9811788, PubMed:12615944). Present also in subcomplex forms which inculdes CSN3 (PubMed:15486099). Also exists as monomeric form (PubMed:9811788). Interacts with CYT1 in vitro, but not in planta (PubMed:23424245).4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi24138. 153 interactors.
IntActiQ8LAZ7. 153 interactors.
STRINGi3702.AT1G22920.1.

Structurei

3D structure databases

ProteinModelPortaliQ8LAZ7.
SMRiQ8LAZ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 196MPNPROSITE-ProRule annotationAdd BLAST138

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi142 – 155JAMM motifPROSITE-ProRule annotationAdd BLAST14

Domaini

The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex (By similarity).By similarity

Sequence similaritiesi

Belongs to the peptidase M67A family. CSN5 subfamily.Curated

Phylogenomic databases

eggNOGiKOG1554. Eukaryota.
COG1310. LUCA.
HOGENOMiHOG000116528.
InParanoidiQ8LAZ7.
KOiK09613.
OMAiKYWVQTL.
OrthoDBiEOG09360E02.
PhylomeDBiQ8LAZ7.

Family and domain databases

InterProiView protein in InterPro
IPR000555. JAMM/MPN+_dom.
PfamiView protein in Pfam
PF01398. JAB. 1 hit.
SMARTiView protein in SMART
SM00232. JAB_MPN. 1 hit.
PROSITEiView protein in PROSITE
PS50249. MPN. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8LAZ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGSSSAIAR KTWELENNIL PVEPTDSASD SIFHYDDASQ AKIQQEKPWA
60 70 80 90 100
SDPNYFKRVH ISALALLKMV VHARSGGTIE IMGLMQGKTE GDTIIVMDAF
110 120 130 140 150
ALPVEGTETR VNAQSDAYEY MVEYSQTSKL AGRLENVVGW YHSHPGYGCW
160 170 180 190 200
LSGIDVSTQM LNQQYQEPFL AVVIDPTRTV SAGKVEIGAF RTYPEGHKIS
210 220 230 240 250
DDHVSEYQTI PLNKIEDFGV HCKQYYSLDI TYFKSSLDSH LLDLLWNKYW
260 270 280 290 300
VNTLSSSPLL GNGDYVAGQI SDLAEKLEQA ESQLANSRYG GIAPAGHQRR
310 320 330 340 350
KEDEPQLAKI TRDSAKITVE QVHGLMSQVI KDILFNSARQ SKKSADDSSD

PEPMITS
Length:357
Mass (Da):39,731
Last modified:November 23, 2004 - v2
Checksum:i08FAC28547FDEEFB
GO
Isoform 2 (identifier: Q8LAZ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     330-357: IKDILFNSARQSKKSADDSSDPEPMITS → SKYNLTMLILFPLAVNGSMKLT

Note: No experimental confirmation available. Derived from EST data.Curated
Show »
Length:351
Mass (Da):39,105
Checksum:iA203F68C7189BD94
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21P → R in AAM65053 (Ref. 7) Curated1
Sequence conflicti246W → G in AAC36344 (PubMed:9811788).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058794330 – 357IKDIL…PMITS → SKYNLTMLILFPLAVNGSMK LT in isoform 2. Add BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087413 mRNA. Translation: AAC36344.1.
AF395062 mRNA. Translation: AAL58105.1.
AF042334 mRNA. Translation: AAB96974.1.
AF000657 Genomic DNA. Translation: AAB72159.1.
CP002684 Genomic DNA. Translation: AEE30309.1.
CP002684 Genomic DNA. Translation: AEE30310.1.
BT029486 mRNA. Translation: ABL66743.1.
AY087510 mRNA. Translation: AAM65053.1.
BT003909 mRNA. Translation: AAO41956.1.
PIRiC86363.
T52180.
RefSeqiNP_173705.1. NM_102139.2. [Q8LAZ7-1]
NP_973890.1. NM_202161.1. [Q8LAZ7-2]
UniGeneiAt.10441.

Genome annotation databases

EnsemblPlantsiAT1G22920.1; AT1G22920.1; AT1G22920. [Q8LAZ7-1]
AT1G22920.2; AT1G22920.2; AT1G22920. [Q8LAZ7-2]
GeneIDi838899.
GrameneiAT1G22920.1; AT1G22920.1; AT1G22920.
AT1G22920.2; AT1G22920.2; AT1G22920.
KEGGiath:AT1G22920.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCSN5A_ARATH
AccessioniPrimary (citable) accession number: Q8LAZ7
Secondary accession number(s): A1A6G7
, F4I325, O23130, O82524, Q84WE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: October 25, 2017
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families