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Protein

COP9 signalosome complex subunit 5b

Gene

CSN5B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex (By similarity). The CSN complex is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of PSIAA6 by regulating the activity of the Ubl ligase SCF-TIR complex.By similarity2 Publications

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi142Zinc; catalyticBy similarity1
Metal bindingi144Zinc; catalyticBy similarity1
Metal bindingi155Zinc; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

  • COP9 signalosome assembly Source: TAIR
  • cullin deneddylation Source: TAIR
  • negative regulation of photomorphogenesis Source: TAIR
  • positive regulation of G2/M transition of mitotic cell cycle Source: TAIR
  • protein deneddylation Source: TAIR
  • red, far-red light phototransduction Source: UniProtKB-KW
  • regulation of defense response Source: TAIR
  • response to auxin Source: TAIR
  • specification of floral organ identity Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Phytochrome signaling pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-5696394. DNA Damage Recognition in GG-NER.
R-ATH-6781823. Formation of TC-NER Pre-Incision Complex.

Protein family/group databases

MEROPSiM67.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
COP9 signalosome complex subunit 5b (EC:3.4.-.-)
Short name:
Signalosome subunit 5b
Alternative name(s):
Jun activation domain-binding homolog 1
Gene namesi
Name:CSN5B
Synonyms:AJH1
Ordered Locus Names:At1g22920
ORF Names:F19G10.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G22920.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signalosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948441 – 357COP9 signalosome complex subunit 5bAdd BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ8LAZ7.
PRIDEiQ8LAZ7.

PTM databases

iPTMnetiQ8LAZ7.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highly expressed in flowers and roots. Expressed at lower level in seedlings and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ8LAZ7. baseline and differential.
GenevisibleiQ8LAZ7. AT.

Interactioni

Subunit structurei

Component of the CSN complex, probably composed of CSN1, CSN2, CSN3, CSN4, CSN5 (CSN5A or CSN5B), CSN6 (CSN6A or CSN6B), CSN7 and CSN8. Interacts with itself. In the complex, it is located in the center and probably interacts directly with CSN4 and CSN6A or CSN6B. Also exists as monomeric form.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CSN1P454323EBI-531132,EBI-530996
CSN6AQ8W2063EBI-531132,EBI-531094

Protein-protein interaction databases

BioGridi24138. 153 interactors.
IntActiQ8LAZ7. 17 interactors.
STRINGi3702.AT1G22920.1.

Structurei

3D structure databases

ProteinModelPortaliQ8LAZ7.
SMRiQ8LAZ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 168MPNAdd BLAST112

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi142 – 155JAMM motifAdd BLAST14

Domaini

The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex (By similarity).By similarity

Sequence similaritiesi

Belongs to the peptidase M67A family. CSN5 subfamily.Curated
Contains 1 MPN (JAB/Mov34) domain.Curated

Phylogenomic databases

eggNOGiKOG1554. Eukaryota.
COG1310. LUCA.
HOGENOMiHOG000116528.
InParanoidiQ8LAZ7.
KOiK09613.
OMAiMQEVQSI.
OrthoDBiEOG09360E02.
PhylomeDBiQ8LAZ7.

Family and domain databases

InterProiIPR000555. JAMM/MPN+_dom.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8LAZ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGSSSAIAR KTWELENNIL PVEPTDSASD SIFHYDDASQ AKIQQEKPWA
60 70 80 90 100
SDPNYFKRVH ISALALLKMV VHARSGGTIE IMGLMQGKTE GDTIIVMDAF
110 120 130 140 150
ALPVEGTETR VNAQSDAYEY MVEYSQTSKL AGRLENVVGW YHSHPGYGCW
160 170 180 190 200
LSGIDVSTQM LNQQYQEPFL AVVIDPTRTV SAGKVEIGAF RTYPEGHKIS
210 220 230 240 250
DDHVSEYQTI PLNKIEDFGV HCKQYYSLDI TYFKSSLDSH LLDLLWNKYW
260 270 280 290 300
VNTLSSSPLL GNGDYVAGQI SDLAEKLEQA ESQLANSRYG GIAPAGHQRR
310 320 330 340 350
KEDEPQLAKI TRDSAKITVE QVHGLMSQVI KDILFNSARQ SKKSADDSSD

PEPMITS
Length:357
Mass (Da):39,731
Last modified:November 23, 2004 - v2
Checksum:i08FAC28547FDEEFB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21P → R in AAM65053 (Ref. 6) Curated1
Sequence conflicti246W → G in AAC36344 (PubMed:9811788).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087413 mRNA. Translation: AAC36344.1.
AF395062 mRNA. Translation: AAL58105.1.
AF042334 mRNA. Translation: AAB96974.1.
AF000657 Genomic DNA. Translation: AAB72159.1.
CP002684 Genomic DNA. Translation: AEE30309.1.
AY087510 mRNA. Translation: AAM65053.1.
BT003909 mRNA. Translation: AAO41956.1.
PIRiC86363.
T52180.
RefSeqiNP_173705.1. NM_102139.2. [Q8LAZ7-1]
UniGeneiAt.10441.

Genome annotation databases

EnsemblPlantsiAT1G22920.1; AT1G22920.1; AT1G22920. [Q8LAZ7-1]
GeneIDi838899.
GrameneiAT1G22920.1; AT1G22920.1; AT1G22920.
KEGGiath:AT1G22920.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087413 mRNA. Translation: AAC36344.1.
AF395062 mRNA. Translation: AAL58105.1.
AF042334 mRNA. Translation: AAB96974.1.
AF000657 Genomic DNA. Translation: AAB72159.1.
CP002684 Genomic DNA. Translation: AEE30309.1.
AY087510 mRNA. Translation: AAM65053.1.
BT003909 mRNA. Translation: AAO41956.1.
PIRiC86363.
T52180.
RefSeqiNP_173705.1. NM_102139.2. [Q8LAZ7-1]
UniGeneiAt.10441.

3D structure databases

ProteinModelPortaliQ8LAZ7.
SMRiQ8LAZ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24138. 153 interactors.
IntActiQ8LAZ7. 17 interactors.
STRINGi3702.AT1G22920.1.

Protein family/group databases

MEROPSiM67.A01.

PTM databases

iPTMnetiQ8LAZ7.

Proteomic databases

PaxDbiQ8LAZ7.
PRIDEiQ8LAZ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G22920.1; AT1G22920.1; AT1G22920. [Q8LAZ7-1]
GeneIDi838899.
GrameneiAT1G22920.1; AT1G22920.1; AT1G22920.
KEGGiath:AT1G22920.

Organism-specific databases

TAIRiAT1G22920.

Phylogenomic databases

eggNOGiKOG1554. Eukaryota.
COG1310. LUCA.
HOGENOMiHOG000116528.
InParanoidiQ8LAZ7.
KOiK09613.
OMAiMQEVQSI.
OrthoDBiEOG09360E02.
PhylomeDBiQ8LAZ7.

Enzyme and pathway databases

ReactomeiR-ATH-5696394. DNA Damage Recognition in GG-NER.
R-ATH-6781823. Formation of TC-NER Pre-Incision Complex.

Miscellaneous databases

PROiQ8LAZ7.

Gene expression databases

ExpressionAtlasiQ8LAZ7. baseline and differential.
GenevisibleiQ8LAZ7. AT.

Family and domain databases

InterProiIPR000555. JAMM/MPN+_dom.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSN5B_ARATH
AccessioniPrimary (citable) accession number: Q8LAZ7
Secondary accession number(s): O23130, O82524, Q84WE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: November 30, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.