Q8LAW2 (AFR_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box protein AFR

Alternative name(s):
Protein ATTENUATED FAR-RED RESPONSE
SKP1-interacting partner 29

Gene names

Name:	AFR
Synonyms:	SKIP29
Ordered Locus Names:	At2g24540
ORF Names:	F25P17.16

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of SCF (ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins By similarity. Part of the phyA-mediated signaling transduction pathway leading to the regulation of gene expression and hypocotyls elongation in response to red and far-red light exposure. Ref.6
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (ASK-cullin-F-box) protein ligase complex By similarity. Interacts with SKP1A. Ref.6 Ref.7
Induction	Rhythmic expression with highest levels at the end of the dark phase, just before the transition to light. Induction by far-red light requires phyA. Ref.5 Ref.6
Domain	The kelch repeats form a beta-propeller structure involved in protein-protein interaction. The F-box is required for the interaction with SKP1A.
Sequence similarities	Contains 1 F-box domain. Contains 5 Kelch repeats.

Ontologies

Keywords
Biological process	Phytochrome signaling pathway Ubl conjugation pathway
Domain	Kelch repeat Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	red, far-red light phototransduction Inferred from mutant phenotype Ref.6. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction Ref.6. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKP1A	Q39255	4	EBI-604438,EBI-532357

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	F-box protein AFR		PRO_0000119959
Regions
	Domain	29 – 74	46	F-box
	Repeat	80 – 126	47	Kelch 1
	Repeat	135 – 178	44	Kelch 2
	Repeat	179 – 227	49	Kelch 3
	Repeat	229 – 276	48	Kelch 4
	Repeat	279 – 325	47	Kelch 5
Experimental info
	Sequence conflict	130	1	M → I in AAM65112. Ref.4
	Sequence conflict	246	1	Q → E in AAM65112. Ref.4
	Sequence conflict	307	1	Q → P in AAM65112. Ref.4
	Sequence conflict	351	1	R → K in AAM65112. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8LAW2 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: 0F48D9747B900634	FASTA	372	41,278
	10 20 30 40 50 60 MAEQETTSNI NTINDQAEEE TRTKSQPLIS GLPNDIAELC LLRLPYPYHA LYRSVSSSWN 70 80 90 100 110 120 KTITNPRFLF SKQSLSISSP YLFVFAFNKS TARIQWQSLD LASGRWFVLP PMPNSFTKIS 130 140 150 160 170 180 SPHALSCASM PRQGKLFVLG GGDVNRSAVV YTALTNRWSC ISPMMSPRTY FVSGNVNGKI 190 200 210 220 230 240 MAVGGSVGGN GEATTEVESY DPDNDTWTVV KKLPMVLAKY DSAVIGKEMC VTEGWAWPFM 250 260 270 280 290 300 FPPMGQVYDS DEGTWREMSG GMKEGWTGVS VVIRDRLFVI SEHGDFPMKV YCSDDDTWRY 310 320 330 340 350 360 VSGEKLQGEK MRRPFAVTGA DDRVFVVASG INVAEGRVSE GQNGDFSVEW RMVSSPKSSI 370 QFSPASCHVL YV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Orchestrated transcription of key pathways in Arabidopsis by the circadian clock." Harmer S.L., Hogenesch J.B., Straume M., Chang H.-S., Han B., Zhu T., Wang X., Kreps J.A., Kay S.A. Science 290:2110-2113(2000) [PubMed: 11118138] [Abstract] Cited for: INDUCTION.
[6]	"The F box protein AFR is a positive regulator of phytochrome A-mediated light signaling." Harmon F.G., Kay S.A. Curr. Biol. 13:2091-2096(2003) [PubMed: 14653999] [Abstract] Cited for: FUNCTION, INDUCTION, INTERACTION WITH SKP1A/ASK1.
[7]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006954 Genomic DNA. Translation: AAD23891.1. CP002685 Genomic DNA. Translation: AEC07590.1. AY072009 mRNA. Translation: AAL57704.1. BT000847 mRNA. Translation: AAN38684.1. AY087570 mRNA. Translation: AAM65112.1.
IPI	IPI00535909.
PIR	H84637.
RefSeq	NP_565572.1. NM_128015.2.
UniGene	At.22510. At.28274.
3D structure databases
ProteinModelPortal	Q8LAW2.
SMR	Q8LAW2. Positions 28-272.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8LAW2. 6 interactions.
STRING	Q8LAW2.
Proteomic databases
PRIDE	Q8LAW2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G24540.1; AT2G24540.1; AT2G24540.
GeneID	816990.
GenomeReviews	Gene locus AT2G24540 in contig CT485783_GR.
KEGG	ath:AT2G24540.
NMPDR	fig|3702.1.peg.9478.
Organism-specific databases
TAIR	At2g24540.
Phylogenomic databases
eggNOG	KOG1072.
GeneTree	EPGT00070000029589.
HOGENOM	HBG600358.
InParanoid	Q8LAW2.
OMA	GRWFVLP.
PhylomeDB	Q8LAW2.
ProtClustDB	CLSN2688094.
Gene expression databases
Genevestigator	Q8LAW2.
GermOnline	AT2G24540. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001810. F-box_dom_cyclin-like. IPR022364. F-box_dom_Skp2-like. IPR015916. Gal_Oxidase_b-propeller. IPR006652. Kelch_1. [Graphical view]
Gene3D	G3DSA:2.130.10.80. Gal_Oxidase_b-propeller. 1 hit.
Pfam	PF01344. Kelch_1. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. SM00612. Kelch. 2 hits. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit.
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AFR_ARATH

Accession

Primary (citable) accession number: Q8LAW2
Secondary accession number(s): Q9SJB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	December 14, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program