Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8LAM0

- UBP4_ARATH

UniProt

Q8LAM0 - UBP4_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

UBP4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Required for the correct development of pollen.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei32 – 321Nucleophile
Active sitei310 – 3101Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT2G22310-MONOMER.
ARA:GQT-1522-MONOMER.

Protein family/group databases

MEROPSiC19.092.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Short name:
AtUBP4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:UBP4
Ordered Locus Names:At2g22310
ORF Names:T26C19.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G22310.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi32 – 321C → S: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedSequence Analysis
Chaini2 – 365364Ubiquitin carboxyl-terminal hydrolase 4PRO_0000080695Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PRIDEiQ8LAM0.

Expressioni

Tissue specificityi

Constitutively and ubiquitously expressed.1 Publication

Gene expression databases

ExpressionAtlasiQ8LAM0. baseline and differential.
GenevestigatoriQ8LAM0.

Structurei

3D structure databases

ProteinModelPortaliQ8LAM0.
SMRiQ8LAM0. Positions 24-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 362340USPAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi81 – 9818Bipartite nuclear localization signalSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000231498.
InParanoidiQ8LAM0.
KOiK11842.
PhylomeDBiQ8LAM0.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8LAM0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAAGSKLEK ALGDQFPEGE RYFGFENFGN TCYCNSVLQA LYFCAPFREQ
60 70 80 90 100
LLEHYANNKA DAEENLLTCL ADLFSQISSQ KKKTGVIAPK RFVQRLKKQN
110 120 130 140 150
ELFRSYMHQD AHEFLNYLLN ELVEILEKET QATKADNETS SSPEKIANVL
160 170 180 190 200
KAPLANGVHK EPIVTWVHKI FQGILTNETR CLRCETVTAR DETFLDLSLD
210 220 230 240 250
IEQNSSITSC LKNFSSTETL HAEDKFFCDK CCSLQEAQKR MKIKKPPHIL
260 270 280 290 300
VIHLKRFKYM EQLGRYKKLS YRVVFPLELK LSNTVDEYVD IEYSLFAVVV
310 320 330 340 350
HVGSGPNHGH YVSLVKSHNH WLFFDDESVE IIEESAVQTF FGSSQEYSSN
360
TDHGYILLYE SLGTR
Length:365
Mass (Da):41,846
Last modified:April 26, 2005 - v2
Checksum:i23E86CF5FDB0CE6D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti172 – 1721Q → K in AAM65268. 1 PublicationCurated
Sequence conflicti334 – 3341E → K in AAM65268. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76846 mRNA. Translation: AAB67967.1.
AC007168 Genomic DNA. Translation: AAD23612.1.
CP002685 Genomic DNA. Translation: AEC07291.1.
AY048261 mRNA. Translation: AAK82523.1.
AY072625 mRNA. Translation: AAL62016.1.
AY087731 mRNA. Translation: AAM65268.1.
PIRiB84611.
RefSeqiNP_565532.1. NM_127796.3. [Q8LAM0-1]
UniGeneiAt.48505.
At.71036.

Genome annotation databases

EnsemblPlantsiAT2G22310.1; AT2G22310.1; AT2G22310. [Q8LAM0-1]
GeneIDi816763.
KEGGiath:AT2G22310.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76846 mRNA. Translation: AAB67967.1 .
AC007168 Genomic DNA. Translation: AAD23612.1 .
CP002685 Genomic DNA. Translation: AEC07291.1 .
AY048261 mRNA. Translation: AAK82523.1 .
AY072625 mRNA. Translation: AAL62016.1 .
AY087731 mRNA. Translation: AAM65268.1 .
PIRi B84611.
RefSeqi NP_565532.1. NM_127796.3. [Q8LAM0-1 ]
UniGenei At.48505.
At.71036.

3D structure databases

ProteinModelPortali Q8LAM0.
SMRi Q8LAM0. Positions 24-339.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C19.092.

Proteomic databases

PRIDEi Q8LAM0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G22310.1 ; AT2G22310.1 ; AT2G22310 . [Q8LAM0-1 ]
GeneIDi 816763.
KEGGi ath:AT2G22310.

Organism-specific databases

GeneFarmi 4912.
TAIRi AT2G22310.

Phylogenomic databases

eggNOGi COG5533.
HOGENOMi HOG000231498.
InParanoidi Q8LAM0.
KOi K11842.
PhylomeDBi Q8LAM0.

Enzyme and pathway databases

BioCyci ARA:AT2G22310-MONOMER.
ARA:GQT-1522-MONOMER.

Gene expression databases

ExpressionAtlasi Q8LAM0. baseline and differential.
Genevestigatori Q8LAM0.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "AtUBP3 and AtUBP4 are two closely related Arabidopsis thaliana ubiquitin-specific proteases present in the nucleus."
    Chandler J.S., McArdle B., Callis J.
    Mol. Gen. Genet. 255:302-310(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-32, TISSUE SPECIFICITY.
    Strain: cv. Landsberg erecta.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Ubiquitin-specific proteases from Arabidopsis thaliana: cloning of AtUBP5 and analysis of substrate specificity of AtUBP3, AtUBP4, and AtUBP5 using Escherichia coli in vivo and in vitro assays."
    Rao-Naik C., Chandler J.S., McArdle B., Callis J.
    Arch. Biochem. Biophys. 379:198-208(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
  8. "Unexpected protein families including cell defense components feature in the N-myristoylome of a higher eukaryote."
    Boisson B., Giglione C., Meinnel T.
    J. Biol. Chem. 278:43418-43429(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MYRISTOYLATION AT GLY-2.
  9. "The ubiquitin-specific protease subfamily UBP3/UBP4 is essential for pollen development and transmission in Arabidopsis."
    Doelling J.H., Phillips A.R., Soyler-Ogretim G., Wise J., Chandler J.S., Callis J., Otegui M.S., Vierstra R.D.
    Plant Physiol. 145:801-813(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiUBP4_ARATH
AccessioniPrimary (citable) accession number: Q8LAM0
Secondary accession number(s): O24455
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: October 29, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3