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Reviewed, UniProtKB/Swiss-Prot Q8LAH8 (NDK4_ARATH)

Last modified February 9, 2010. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Nucleoside diphosphate kinase IV, chloroplastic/mitochondrial
      Short name=NDP kinase IV
      Short name=NDPK IV
      Short name=NDK IV
    EC=2.7.4.6
Alternative name(s):
    Nucleoside diphosphate kinase 4
Gene names
Name: NDK4
Ordered Locus Names: At4g23900
ORF Names: T32A16.70
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Shows the highest specificity towards GDP By similarity.

Catalytic activity

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Cofactor

Magnesium By similarity.

Subunit structure

Homohexamer Probable.

Subcellular location

Plastidchloroplast thylakoid lumen By similarity. Mitochondrion intermembrane space By similarity Ref.5 Ref.6.

Sequence similarities

Belongs to the NDK family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 8484Chloroplast and mitochondrion By similarity
Chain85 – 237153Nucleoside diphosphate kinase IV, chloroplastic/mitochondrial
PRO_0000019436

Sites

Active site2011Pros-phosphohistidine intermediate By similarity
Binding site951ATP By similarity
Binding site1431ATP By similarity
Binding site1711ATP By similarity
Binding site1771ATP By similarity
Binding site1881ATP By similarity
Binding site1981ATP By similarity

Experimental info

Sequence conflict361S → T in AAM65336. Ref.4
Sequence conflict661G → R in AAM65336. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q8LAH8-1 [UniParc].

Last modified March 15, 2004. Version 2.
Checksum: 0CC2B487E3D376FA

FASTA23725,773
        10         20         30         40         50         60 
MSSQICRSAS RAARSLLSSA KNARFFSEGR AIGAASVVHA TGKVPQYASN FGKSGSGFVS 

        70         80         90        100        110        120 
NSWITGLLAL PAAAFMLQDQ EALAAEMERT FIAIKPDGVQ RGLISEIITR FERKGYKLVG 

       130        140        150        160        170        180 
IKVMVPSKGF AQKHYHDLKE RPFFNGLCNF LSSGPVVAMV WEGEGVIRYG RKLIGATDPQ 

       190        200        210        220        230 
KSEPGTIRGD LAVVVGRNII HGSDGPETAK DEISLWFKPE ELVSYTSNAE KWIYGQN 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis NDP kinase gene NDK4."
Yabe N., Hashimoto K., Hasunuma K.
Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed: 10617198] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed: 11910074] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Proteomic approach to identify novel mitochondrial proteins in Arabidopsis."
Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P.
Plant Physiol. 127:1694-1710(2001) [PubMed: 11743114] [Abstract]
Cited for: PROTEIN SEQUENCE OF 85-102, SUBCELLULAR LOCATION.
Tissue: Leaf and Stem.
[6]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL161560 Genomic DNA. Translation: CAB81308.1.
AL078468 Genomic DNA. Translation: CAB43890.1.
AB044970 Genomic DNA. Translation: BAB19789.1.
AK117895 mRNA. Translation: BAC42534.1.
AY087800 mRNA. Translation: AAM65336.1.
IPIIPI00542543.
PIRT08909.
RefSeqNP_567690.1.
UniGeneAt.32397
Rra.16635
Rsa.15627

3D structure databases

SMRQ8LAH8. Positions 85-235.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8LAH8.

Proteomic databases

PRIDEQ8LAH8.

Genome annotation databases

GeneID828490.
GenomeReviewsGene locus AT4G23900 in contig CT486007_GR.
KEGGath:AT4G23900.
NMPDRfig|3702.1.peg.20272.

Organism-specific databases

GeneFarm1972. 147.
TAIRAt4g23900.

Phylogenomic databases

HOGENOMHBG445152.
InParanoidQ8LAH8.
OMAVAIKIVV.
PhylomeDBQ8LAH8.

Enzyme and pathway databases

BRENDA2.7.4.6. 302.

Gene expression databases

ArrayExpressQ8LAH8.
GenevestigatorQ8LAH8.
GermOnlineAT4G23900. Arabidopsis thaliana.

Family and domain databases

InterProIPR001564. Nuc_diP_kinase_core.
[Graphical view]
Gene3DG3DSA:3.30.70.141. NDK. 1 hit.
PANTHERPTHR11349. Nuc_diP_kinase_core. 1 hit.
PfamPF00334. NDK. 1 hit.
[Graphical view]
PRINTSPR01243. NUCDPKINASE.
SMARTSM00562. NDK. 1 hit.
[Graphical view]
PROSITEPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDK4_ARATH
AccessionPrimary (citable) accession number: Q8LAH8
Secondary accession number(s): Q9T0A5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: February 9, 2010
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents