Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nucleoside diphosphate kinase IV, chloroplastic/mitochondrial

Gene

NDK4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Shows the highest specificity towards GDP (By similarity).By similarity

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95ATPBy similarity1
Binding sitei143ATPBy similarity1
Binding sitei171ATPBy similarity1
Binding sitei177ATPBy similarity1
Binding sitei188ATPBy similarity1
Binding sitei198ATPBy similarity1
Active sitei201Pros-phosphohistidine intermediateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • nucleoside diphosphate kinase activity Source: GO_Central
  • zinc ion binding Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G23900-MONOMER.
ReactomeiR-ATH-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase IV, chloroplastic/mitochondrial (EC:2.7.4.6)
Short name:
NDK IV
Short name:
NDP kinase IV
Short name:
NDPK IV
Alternative name(s):
Nucleoside diphosphate kinase 4
Gene namesi
Name:NDK4
Ordered Locus Names:At4g23900
ORF Names:T32A16.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G23900.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 84Chloroplast and mitochondrionBy similarityAdd BLAST84
ChainiPRO_000001943685 – 237Nucleoside diphosphate kinase IV, chloroplastic/mitochondrialAdd BLAST153

Proteomic databases

PaxDbiQ8LAH8.
PRIDEiQ8LAH8.

PTM databases

iPTMnetiQ8LAH8.

Expressioni

Gene expression databases

ExpressionAtlasiQ8LAH8. baseline and differential.
GenevisibleiQ8LAH8. AT.

Interactioni

Subunit structurei

Homohexamer.Curated

Protein-protein interaction databases

STRINGi3702.AT4G23900.1.

Structurei

3D structure databases

ProteinModelPortaliQ8LAH8.
SMRiQ8LAH8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NDK family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
HOGENOMiHOG000224564.
InParanoidiQ8LAH8.
KOiK00940.
OrthoDBiEOG09360LYS.
PhylomeDBiQ8LAH8.

Family and domain databases

HAMAPiMF_00451. NDP_kinase. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8LAH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSQICRSAS RAARSLLSSA KNARFFSEGR AIGAASVVHA TGKVPQYASN
60 70 80 90 100
FGKSGSGFVS NSWITGLLAL PAAAFMLQDQ EALAAEMERT FIAIKPDGVQ
110 120 130 140 150
RGLISEIITR FERKGYKLVG IKVMVPSKGF AQKHYHDLKE RPFFNGLCNF
160 170 180 190 200
LSSGPVVAMV WEGEGVIRYG RKLIGATDPQ KSEPGTIRGD LAVVVGRNII
210 220 230
HGSDGPETAK DEISLWFKPE ELVSYTSNAE KWIYGQN
Length:237
Mass (Da):25,773
Last modified:March 15, 2004 - v2
Checksum:i0CC2B487E3D376FA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36S → T in AAM65336 (Ref. 5) Curated1
Sequence conflicti66G → R in AAM65336 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL078468 Genomic DNA. Translation: CAB43890.1.
AL161560 Genomic DNA. Translation: CAB81308.1.
CP002687 Genomic DNA. Translation: AEE84824.1.
AB044970 Genomic DNA. Translation: BAB19789.1.
AK117895 mRNA. Translation: BAC42534.1.
AY087800 mRNA. Translation: AAM65336.1.
PIRiT08909.
RefSeqiNP_567690.1. NM_118522.3.
UniGeneiAt.32397.

Genome annotation databases

EnsemblPlantsiAT4G23900.1; AT4G23900.1; AT4G23900.
GeneIDi828490.
GrameneiAT4G23900.1; AT4G23900.1; AT4G23900.
KEGGiath:AT4G23900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL078468 Genomic DNA. Translation: CAB43890.1.
AL161560 Genomic DNA. Translation: CAB81308.1.
CP002687 Genomic DNA. Translation: AEE84824.1.
AB044970 Genomic DNA. Translation: BAB19789.1.
AK117895 mRNA. Translation: BAC42534.1.
AY087800 mRNA. Translation: AAM65336.1.
PIRiT08909.
RefSeqiNP_567690.1. NM_118522.3.
UniGeneiAt.32397.

3D structure databases

ProteinModelPortaliQ8LAH8.
SMRiQ8LAH8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G23900.1.

PTM databases

iPTMnetiQ8LAH8.

Proteomic databases

PaxDbiQ8LAH8.
PRIDEiQ8LAH8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G23900.1; AT4G23900.1; AT4G23900.
GeneIDi828490.
GrameneiAT4G23900.1; AT4G23900.1; AT4G23900.
KEGGiath:AT4G23900.

Organism-specific databases

TAIRiAT4G23900.

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
HOGENOMiHOG000224564.
InParanoidiQ8LAH8.
KOiK00940.
OrthoDBiEOG09360LYS.
PhylomeDBiQ8LAH8.

Enzyme and pathway databases

BioCyciARA:AT4G23900-MONOMER.
ReactomeiR-ATH-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ8LAH8.

Gene expression databases

ExpressionAtlasiQ8LAH8. baseline and differential.
GenevisibleiQ8LAH8. AT.

Family and domain databases

HAMAPiMF_00451. NDP_kinase. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDK4_ARATH
AccessioniPrimary (citable) accession number: Q8LAH8
Secondary accession number(s): Q9T0A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: November 30, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.