Reviewed, UniProtKB/Swiss-Prot Q8L9J9 (CAES_ARATH)

Names and origin

Protein names

Recommended name:
    Probable carbohydrate esterase At4g34215
    EC=3.1.-.-

Gene names

Ordered Locus Names:	At4g34215
ORF Names:	F10M10.12, F28A23_20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities	Belongs to the carbohydrate esterase 6 family.
Sequence caution	The sequence CAA17550.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At4g34220 has been split into 2 genes: At4g34215 and At4g34220. The sequence CAB80139.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At4g34220 has been split into 2 genes: At4g34215 and At4g34220.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 260

260

Probable carbohydrate esterase At4g34215

PRO_0000238463

Sites

Active site

31

1

Active site

235

1

Active site

238

1

Experimental info

Sequence conflict

4

1

G → E in AAM65927. Ref.2

Sequence conflict

8

1

P → S in AAM65927. Ref.2

Sequence conflict

40

1

F → V in AAM65927. Ref.2

Sequence conflict

45

1

N → H in AAM65927. Ref.2

Sequence conflict

106

1

L → V in AAM65927. Ref.2

Secondary structure

1

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260

Helix Strand Turn

Details...

Beta strand

22 – 30

9

Helix

31 – 34

4

Beta strand

39 – 41

3

Turn

43 – 45

3

Beta strand

48 – 50

3

Helix

56 – 58

3

Beta strand

64 – 67

4

Beta strand

73 – 75

3

Turn

80 – 84

5

Helix

95 – 106

12

Beta strand

113 – 117

5

Helix

125 – 128

4

Helix

133 – 145

13

Helix

146 – 148

3

Beta strand

151 – 158

8

Helix

161 – 163

3

Beta strand

164 – 166

3

Helix

167 – 187

21

Beta strand

195 – 199

5

Helix

207 – 216

10

Beta strand

222 – 226

5

Beta strand

236 – 239

4

Helix

241 – 259

19

Sequences

	Sequence		Length	Mass (Da)
	Q8L9J9-1 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: 90712C57F871C742	FASTA	260	28,358
	10 20 30 40 50 60 MEGGSITPGE DKPEIQSPIP PNQIFILSGQ SNMAGRGGVF KDHHNNRWVW DKILPPECAP 70 80 90 100 110 120 NSSILRLSAD LRWEEAHEPL HVDIDTGKVC GVGPGMAFAN AVKNRLETDS AVIGLVPCAS 130 140 150 160 170 180 GGTAIKEWER GSHLYERMVK RTEESRKCGG EIKAVLWYQG ESDVLDIHDA ESYGNNMDRL 190 200 210 220 230 240 IKNLRHDLNL PSLPIIQVAI ASGGGYIDKV REAQLGLKLS NVVCVDAKGL PLKSDNLHLT 250 260 TEAQVQLGLS LAQAYLSNFC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Full-length cDNA from Arabidopsis thaliana." Brover V., Troukhan M., Alexandrov N., Lu Y.-P., Flavell R., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The structure at 1.6 Angstroms resolution of the protein product of the At4g34215 gene from Arabidopsis thaliana." Bitto E., Bingman C.A., McCoy J.G., Allard S.T., Wesenberg G.E., Phillips G.N. Jr. Acta Crystallogr. D 61:1655-1661(2005) [PubMed: 16301800] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 1-260.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AL021961 Genomic DNA. Translation: CAA17550.1. Sequence problems.
AL161585 Genomic DNA. Translation: CAB80139.1. Sequence problems.
AY088389 mRNA. Translation: AAM65927.1.

IPI

IPI00522520.

PIR

T05414.

RefSeq

NP_567960.1.
NP_849493.1.

UniGene

At.27025

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2APJ	X-ray	1.60	A/B/C/D	1-260	[»]

ModBase

Search...

Proteomic databases

PRIDE

Q8L9J9.

Genome annotation databases

GeneID

829570.

GenomeReviews

Gene locus AT4G34215 in contig CT486007_GR.

KEGG

ath:AT4G34215.

NMPDR

fig|3702.1.peg.21482.

Organism-specific databases

TAIR

At4g34215.

Phylogenomic databases

OMA

Q8L9J9. LIPCAEG.

Gene expression databases

GermOnline

AT4G34215. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR005181. DUF303_acetylest.
[Graphical view]

Pfam

PF03629. DUF303. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CAES_ARATH

Accession

Primary (citable) accession number: Q8L9J9
Secondary accession number(s): O49483

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	May 30, 2006
Last modified:	May 5, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)