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Q8L9A9 (XTH8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 8

Short name=At-XTH8
Short name=XTH-8
EC=2.4.1.207
Gene names
Name:XTH8
Ordered Locus Names:At1g11545
ORF Names:T23J18.21
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Sequence caution

The sequence AAF16642.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAM66078.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 305274Probable xyloglucan endotransglucosylase/hydrolase protein 8
PRO_0000011808

Sites

Active site1151Nucleophile By similarity
Active site1191Proton donor By similarity

Amino acid modifications

Glycosylation611N-linked (GlcNAc...) Potential
Glycosylation661N-linked (GlcNAc...) Potential
Glycosylation1231N-linked (GlcNAc...) Potential
Glycosylation1381N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8L9A9 [UniParc].

Last modified May 31, 2011. Version 2.
Checksum: 7A5FDFDDC655ECF7

FASTA30535,565
        10         20         30         40         50         60 
METERRIITS CSAMTALFLF MTALMASSSI AATPTQSFED NFNIMWSENH FTTSDDGEIW 

        70         80         90        100        110        120 
NLSLDNDTGC GFQTKHMYRF GWFSMKLKLV GGDSAGVVTA YYMCSENGAG PERDEIDFEF 

       130        140        150        160        170        180 
LGNRTGQPYI IQTNVYKNGT GNREMRHSLW FDPTKDYHTY SILWNNHQLV FFVDRVPIRV 

       190        200        210        220        230        240 
YKNSDKVPNN DFFPNQKPMY LFSSIWNADD WATRGGLEKT DWKKAPFVSS YKDFAVEGCR 

       250        260        270        280        290        300 
WKDPFPACVS TTTENWWDQY DAWHLSKTQK MDYAWVQRNL VVYDYCKDSE RFPTLPWECS 


ISPWA 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
Rose J.K.C., Braam J., Fry S.C., Nishitani K.
Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC011661 Genomic DNA. Translation: AAF16642.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28750.1.
AK228427 mRNA. Translation: BAF00360.1.
AY088546 mRNA. Translation: AAM66078.1. Different initiation.
PIRG86248.
RefSeqNP_563892.1. NM_101028.3.
UniGeneAt.47525.

3D structure databases

ProteinModelPortalQ8L9A9.
SMRQ8L9A9. Positions 38-299.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G11545.1-P.

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbQ8L9A9.
PRIDEQ8L9A9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G11545.1; AT1G11545.1; AT1G11545.
GeneID837698.
KEGGath:AT1G11545.

Organism-specific databases

TAIRAT1G11545.

Phylogenomic databases

eggNOGCOG2273.
HOGENOMHOG000236368.
InParanoidQ8L9A9.
KOK08235.
OMAREMRHSL.

Enzyme and pathway databases

BioCycARA:AT1G11545-MONOMER.

Gene expression databases

GenevestigatorQ8L9A9.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008264. Beta_glucanase.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
PRINTSPR00737. GLHYDRLASE16.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTH8_ARATH
AccessionPrimary (citable) accession number: Q8L9A9
Secondary accession number(s): Q0WR89, Q9LPY1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: May 31, 2011
Last modified: June 11, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names