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Protein

Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG3

Gene

LOG3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms.1 Publication

Catalytic activityi

N(6)-(Delta(2)-isopentenyl)-adenosine 5'-phosphate + H20 = N(6)-(dimethylallyl)adenine + D-ribose 5'-phosphate.1 Publication
Trans-zeatin riboside phosphate + H2O = trans-zeatin + D-ribose 5'-phosphate.1 Publication

Kineticsi

  1. KM=14 µM for N(6)-(Delta(2)-isopentenyl)-adenosine 5'-phosphate1 Publication
  1. Vmax=1.5 µmol/min/mg enzyme with N(6)-(Delta(2)-isopentenyl)-adenosine 5'-phosphate as substrate1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84SubstrateBy similarity1
Binding sitei131SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cytokinin biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG3 (EC:3.2.2.n1)
Alternative name(s):
Protein LONELY GUY 3
Gene namesi
Name:LOG3
Ordered Locus Names:At2g37210
ORF Names:T2N18.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G37210.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions; due to the redundancy with other LOG proteins.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003950461 – 215Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG3Add BLAST215

Expressioni

Tissue specificityi

Expressed in roots and shoots. Detected in root procambium, lateral root primordia, vascular tissues of immature leaves, axillary buds, style and ovular funiculus.1 Publication

Gene expression databases

ExpressionAtlasiQ8L8B8. baseline and differential.
GenevisibleiQ8L8B8. AT.

Interactioni

Protein-protein interaction databases

BioGridi3642. 2 interactors.

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 19Combined sources6
Helixi28 – 43Combined sources16
Beta strandi47 – 50Combined sources4
Helixi56 – 67Combined sources12
Beta strandi72 – 79Combined sources8
Beta strandi92 – 98Combined sources7
Helixi99 – 108Combined sources10
Beta strandi111 – 115Combined sources5
Helixi120 – 134Combined sources15
Beta strandi142 – 145Combined sources4
Helixi147 – 149Combined sources3
Helixi152 – 163Combined sources12
Helixi169 – 172Combined sources4
Beta strandi175 – 180Combined sources6
Helixi181 – 189Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A33X-ray1.95A/B1-215[»]
2Q4OX-ray1.95A/B1-215[»]
ProteinModelPortaliQ8L8B8.
SMRiQ8L8B8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8L8B8.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni102 – 103Substrate bindingBy similarity2
Regioni119 – 125Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the LOG family.Curated

Phylogenomic databases

HOGENOMiHOG000156894.
PhylomeDBiQ8L8B8.

Family and domain databases

InterProiIPR005269. LOG.
IPR031100. LOG_fam.
[Graphical view]
PfamiPF03641. Lysine_decarbox. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00730. TIGR00730. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8L8B8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEIKGESMQK SKFRRICVFC GSSQGKKSSY QDAAVDLGNE LVSRNIDLVY
60 70 80 90 100
GGGSIGLMGL VSQAVHDGGR HVIGIIPKTL MPRELTGETV GEVRAVADMH
110 120 130 140 150
QRKAEMAKHS DAFIALPGGY GTLEELLEVI TWAQLGIHDK PVGLLNVDGY
160 170 180 190 200
YNSLLSFIDK AVEEGFISPT AREIIVSAPT AKELVKKLEE YAPCHERVAT
210
KLCWEMERIG YSSEE
Length:215
Mass (Da):23,558
Last modified:October 1, 2002 - v1
Checksum:i4782E3570DE4B1FF
GO

Sequence cautioni

The sequence AAD18138 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006260 Genomic DNA. Translation: AAD18138.2. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09367.1.
AY102554 mRNA. Translation: AAM76759.1.
AY954818 mRNA. Translation: AAX55144.1.
PIRiH84789.
RefSeqiNP_181258.2. NM_129277.3. [Q8L8B8-1]
UniGeneiAt.37455.

Genome annotation databases

EnsemblPlantsiAT2G37210.1; AT2G37210.1; AT2G37210. [Q8L8B8-1]
GeneIDi818298.
GrameneiAT2G37210.1; AT2G37210.1; AT2G37210.
KEGGiath:AT2G37210.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006260 Genomic DNA. Translation: AAD18138.2. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09367.1.
AY102554 mRNA. Translation: AAM76759.1.
AY954818 mRNA. Translation: AAX55144.1.
PIRiH84789.
RefSeqiNP_181258.2. NM_129277.3. [Q8L8B8-1]
UniGeneiAt.37455.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A33X-ray1.95A/B1-215[»]
2Q4OX-ray1.95A/B1-215[»]
ProteinModelPortaliQ8L8B8.
SMRiQ8L8B8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3642. 2 interactors.

Protocols and materials databases

DNASUi818298.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G37210.1; AT2G37210.1; AT2G37210. [Q8L8B8-1]
GeneIDi818298.
GrameneiAT2G37210.1; AT2G37210.1; AT2G37210.
KEGGiath:AT2G37210.

Organism-specific databases

TAIRiAT2G37210.

Phylogenomic databases

HOGENOMiHOG000156894.
PhylomeDBiQ8L8B8.

Miscellaneous databases

EvolutionaryTraceiQ8L8B8.
PROiQ8L8B8.

Gene expression databases

ExpressionAtlasiQ8L8B8. baseline and differential.
GenevisibleiQ8L8B8. AT.

Family and domain databases

InterProiIPR005269. LOG.
IPR031100. LOG_fam.
[Graphical view]
PfamiPF03641. Lysine_decarbox. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00730. TIGR00730. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLOG3_ARATH
AccessioniPrimary (citable) accession number: Q8L8B8
Secondary accession number(s): Q9ZQD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.