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Q8L850

- PI5K9_ARATH

UniProt

Q8L850 - PI5K9_ARATH

Protein

Phosphatidylinositol 4-phosphate 5-kinase 9

Gene

PIP5K9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (25 Oct 2005)
      Previous versions | rss
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    Functioni

    Plays a role in sugar-mediated root development. Interaction with CINV1 induces repression of CINV1 activity and negative regulation of sugar-mediated root cell elongation.1 Publication

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

    GO - Molecular functioni

    1. 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. carbohydrate metabolic process Source: TAIR
    2. cellular amino acid metabolic process Source: TAIR

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G09920-MONOMER.
    ARA:GQT-1409-MONOMER.
    ARA:GQT-2574-MONOMER.
    BRENDAi2.7.1.68. 399.
    ReactomeiREACT_187642. Synthesis of PIPs at the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 4-phosphate 5-kinase 9 (EC:2.7.1.68)
    Short name:
    AtPIP5K9
    Alternative name(s):
    1-phosphatidylinositol 4-phosphate kinase 9
    Diphosphoinositide kinase 9
    PtdIns(4)P-5-kinase 9
    Gene namesi
    Name:PIP5K9
    Ordered Locus Names:At3g09920
    ORF Names:F8A24.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G09920.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. membrane Source: TAIR
    3. nucleus Source: TAIR

    Keywords - Cellular componenti

    Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 815815Phosphatidylinositol 4-phosphate 5-kinase 9PRO_0000185481Add
    BLAST

    Proteomic databases

    PaxDbiQ8L850.
    PRIDEiQ8L850.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Inductioni

    Transiently down-regulated by cold.1 Publication

    Gene expression databases

    ArrayExpressiQ8L850.
    GenevestigatoriQ8L850.

    Interactioni

    Subunit structurei

    Interacts with CINV1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CINV1Q9LQF26EBI-2008013,EBI-2008033

    Protein-protein interaction databases

    BioGridi5485. 1 interaction.
    IntActiQ8L850. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L850.
    SMRiQ8L850. Positions 451-641.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati58 – 8023MORN 1Add
    BLAST
    Repeati81 – 10323MORN 2Add
    BLAST
    Repeati104 – 12623MORN 3Add
    BLAST
    Repeati127 – 14923MORN 4Add
    BLAST
    Repeati150 – 17223MORN 5Add
    BLAST
    Repeati173 – 19523MORN 6Add
    BLAST
    Repeati196 – 21823MORN 7Add
    BLAST
    Repeati219 – 24022MORN 8Add
    BLAST
    Domaini391 – 809419PIPKPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni769 – 79022Activation loopBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 8 MORN repeats.Curated
    Contains 1 PIPK domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG4642.
    HOGENOMiHOG000193875.
    InParanoidiQ8L850.
    KOiK00889.
    OMAiIQKREVR.
    PhylomeDBiQ8L850.

    Family and domain databases

    Gene3Di3.30.800.10. 1 hit.
    3.30.810.10. 2 hits.
    InterProiIPR003409. MORN.
    IPR017163. PIno-4-P-5_kinase_pln.
    IPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view]
    PANTHERiPTHR23086. PTHR23086. 1 hit.
    PfamiPF02493. MORN. 8 hits.
    PF01504. PIP5K. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037274. PIP5K_plant_prd. 1 hit.
    SMARTiSM00698. MORN. 8 hits.
    SM00330. PIPKc. 1 hit.
    [Graphical view]
    PROSITEiPS51455. PIPK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8L850-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGLDVRGAV SFAERTKSVD ALTKKEILSA LNSGEVSETS EDARFRVREL    50
    VLPDGESYSG SLLGNVPEGP GKYIWSDGCV YDGEWRRGMR HGIGNMRWAS 100
    GASYDGEFSG GYMHGSGTYV DANKLTYKGR WRLNLKHGLG YQVYPNGDVF 150
    EGSWIQGLGE GPGKYTWANK NVYLGDMKGG KMSGKGTLTW VTGDSYEGSW 200
    LNGMMHGVGV YTWSDGGCYV GTWTRGLKDG KGSFYSAGTR VPVVQEFYLN 250
    ALRKRGVLPD MRRQNQVASS VNMENLRVGV NRNKLSKGSL INLEQSRNGR 300
    VSLERRWSLE VSIEKVIGHG YSDLSTAVLD SGSSVQYKAN IPILEREYMQ 350
    GVLISELVVN NGFSRTSRRA KRKHKRLVKE AKKPGEVVIK GHRSYDLMLS 400
    LQLGIRYTVG KITPIQRRQV RTADFGPRAS FWMTFPRAGS TMTPPHHSED 450
    FKWKDYCPMV FRNLREMFKI DAADYMMSIC GNDTLRELSS PGKSGSVFFL 500
    SQDDRFMIKT LRKSEVKVLL RMLPDYHHHV KTYENTLITK FFGLHRIKPS 550
    SGQKFRFVVM GNMFFTDLRI HRRFDLKGSS LGRSADKVEI DENTILKDLD 600
    LNYSFFLETS WREGLLRQLE IDSKFLEAQN IMDYSLLLGV HHRAPQHLRS 650
    QLVRSQSITT DALESVAEDD TIEDDMLSYH EGLVLVPRGS ENTVTGPHIR 700
    GSRLRASAVG DEEVDLLLPG TARLQIQQGV NMPARAELIP GREDKEKQIL 750
    HDCCDVVLYL GIIDILQEYN MTKKIEHAYK SLHFDSLSIS AVDPTFYSQR 800
    FLEFIKKVFP QNNKS 815
    Length:815
    Mass (Da):92,092
    Last modified:October 25, 2005 - v2
    Checksum:i3194F009D2C50130
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti482 – 4821N → K in AAM53300. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ810853 mRNA. Translation: CAH18644.1.
    AC015985 Genomic DNA. Translation: AAF23244.1.
    CP002686 Genomic DNA. Translation: AEE74832.1.
    CP002686 Genomic DNA. Translation: AEE74833.1.
    CP002686 Genomic DNA. Translation: AEE74834.1.
    AY120742 mRNA. Translation: AAM53300.1.
    AK229815 mRNA. Translation: BAF01646.1.
    RefSeqiNP_001030666.1. NM_001035589.2.
    NP_001189852.1. NM_001202923.1.
    NP_187603.1. NM_111827.2.
    UniGeneiAt.40019.

    Genome annotation databases

    EnsemblPlantsiAT3G09920.1; AT3G09920.1; AT3G09920.
    AT3G09920.2; AT3G09920.2; AT3G09920.
    AT3G09920.3; AT3G09920.3; AT3G09920.
    GeneIDi820151.
    KEGGiath:AT3G09920.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ810853 mRNA. Translation: CAH18644.1 .
    AC015985 Genomic DNA. Translation: AAF23244.1 .
    CP002686 Genomic DNA. Translation: AEE74832.1 .
    CP002686 Genomic DNA. Translation: AEE74833.1 .
    CP002686 Genomic DNA. Translation: AEE74834.1 .
    AY120742 mRNA. Translation: AAM53300.1 .
    AK229815 mRNA. Translation: BAF01646.1 .
    RefSeqi NP_001030666.1. NM_001035589.2.
    NP_001189852.1. NM_001202923.1.
    NP_187603.1. NM_111827.2.
    UniGenei At.40019.

    3D structure databases

    ProteinModelPortali Q8L850.
    SMRi Q8L850. Positions 451-641.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 5485. 1 interaction.
    IntActi Q8L850. 1 interaction.

    Proteomic databases

    PaxDbi Q8L850.
    PRIDEi Q8L850.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G09920.1 ; AT3G09920.1 ; AT3G09920 .
    AT3G09920.2 ; AT3G09920.2 ; AT3G09920 .
    AT3G09920.3 ; AT3G09920.3 ; AT3G09920 .
    GeneIDi 820151.
    KEGGi ath:AT3G09920.

    Organism-specific databases

    TAIRi AT3G09920.

    Phylogenomic databases

    eggNOGi COG4642.
    HOGENOMi HOG000193875.
    InParanoidi Q8L850.
    KOi K00889.
    OMAi IQKREVR.
    PhylomeDBi Q8L850.

    Enzyme and pathway databases

    BioCyci ARA:AT3G09920-MONOMER.
    ARA:GQT-1409-MONOMER.
    ARA:GQT-2574-MONOMER.
    BRENDAi 2.7.1.68. 399.
    Reactomei REACT_187642. Synthesis of PIPs at the plasma membrane.

    Gene expression databases

    ArrayExpressi Q8L850.
    Genevestigatori Q8L850.

    Family and domain databases

    Gene3Di 3.30.800.10. 1 hit.
    3.30.810.10. 2 hits.
    InterProi IPR003409. MORN.
    IPR017163. PIno-4-P-5_kinase_pln.
    IPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view ]
    PANTHERi PTHR23086. PTHR23086. 1 hit.
    Pfami PF02493. MORN. 8 hits.
    PF01504. PIP5K. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037274. PIP5K_plant_prd. 1 hit.
    SMARTi SM00698. MORN. 8 hits.
    SM00330. PIPKc. 1 hit.
    [Graphical view ]
    PROSITEi PS51455. PIPK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis thaliana putative phosphatidylinositol-4-phosphate 5-kinase."
      Lou Y.
      Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
      Mueller-Roeber B., Pical C.
      Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    7. "PIP5K9, an Arabidopsis phosphatidylinositol monophosphate kinase, interacts with a cytosolic invertase to negatively regulate sugar-mediated root growth."
      Lou Y., Gou J.Y., Xue H.W.
      Plant Cell 19:163-181(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CINV1, TISSUE SPECIFICITY, INDUCTION BY COLD.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiPI5K9_ARATH
    AccessioniPrimary (citable) accession number: Q8L850
    Secondary accession number(s): Q0WMK5, Q9SF93
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: October 25, 2005
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The gain-of-function mutant pip5k9-d (T-DNA insertion line) has reduced primary root length.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3