Reviewed, UniProtKB/Swiss-Prot Q8L850 (PI5K9_ARATH)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol-4-phosphate 5-kinase 9
      Short name=AtPIP5K9
    EC=2.7.1.68
Alternative name(s):
    1-phosphatidylinositol-4-phosphate kinase 9
    PtdIns(4)P-5-kinase 9
    Diphosphoinositide kinase 9

Gene names

Name:	PIP5K9
Ordered Locus Names:	At3g09920
ORF Names:	F8A24.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	815 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
Sequence similarities	Contains 8 MORN repeats. Contains 1 PI5K domain.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from mutant phenotype. Source: TAIR carbohydrate metabolic process Inferred from mutant phenotype. Source: TAIR phosphatidylinositol metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: TAIR membrane Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR
Molecular function	1-phosphatidylinositol-4-phosphate 5-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
At1g35580	Q9LQF2	4	EBI-2008013,EBI-2008033

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 815	815	Phosphatidylinositol-4-phosphate 5-kinase 9		PRO_0000185481
Regions
	Repeat	58 – 80	23	MORN 1
	Repeat	81 – 103	23	MORN 2
	Repeat	104 – 126	23	MORN 3
	Repeat	127 – 149	23	MORN 4
	Repeat	150 – 172	23	MORN 5
	Repeat	173 – 195	23	MORN 6
	Repeat	196 – 218	23	MORN 7
	Repeat	219 – 240	22	MORN 8
	Domain	477 – 809	333	PI5K
	Region	769 – 790	22	Activation loop By similarity
Experimental info
	Sequence conflict	482	1	N → K in AAM53300. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8L850-1 [UniParc]. Last modified October 25, 2005. Version 2. Checksum: 3194F009D2C50130	FASTA	815	92,092
	10 20 30 40 50 60 MSGLDVRGAV SFAERTKSVD ALTKKEILSA LNSGEVSETS EDARFRVREL VLPDGESYSG 70 80 90 100 110 120 SLLGNVPEGP GKYIWSDGCV YDGEWRRGMR HGIGNMRWAS GASYDGEFSG GYMHGSGTYV 130 140 150 160 170 180 DANKLTYKGR WRLNLKHGLG YQVYPNGDVF EGSWIQGLGE GPGKYTWANK NVYLGDMKGG 190 200 210 220 230 240 KMSGKGTLTW VTGDSYEGSW LNGMMHGVGV YTWSDGGCYV GTWTRGLKDG KGSFYSAGTR 250 260 270 280 290 300 VPVVQEFYLN ALRKRGVLPD MRRQNQVASS VNMENLRVGV NRNKLSKGSL INLEQSRNGR 310 320 330 340 350 360 VSLERRWSLE VSIEKVIGHG YSDLSTAVLD SGSSVQYKAN IPILEREYMQ GVLISELVVN 370 380 390 400 410 420 NGFSRTSRRA KRKHKRLVKE AKKPGEVVIK GHRSYDLMLS LQLGIRYTVG KITPIQRRQV 430 440 450 460 470 480 RTADFGPRAS FWMTFPRAGS TMTPPHHSED FKWKDYCPMV FRNLREMFKI DAADYMMSIC 490 500 510 520 530 540 GNDTLRELSS PGKSGSVFFL SQDDRFMIKT LRKSEVKVLL RMLPDYHHHV KTYENTLITK 550 560 570 580 590 600 FFGLHRIKPS SGQKFRFVVM GNMFFTDLRI HRRFDLKGSS LGRSADKVEI DENTILKDLD 610 620 630 640 650 660 LNYSFFLETS WREGLLRQLE IDSKFLEAQN IMDYSLLLGV HHRAPQHLRS QLVRSQSITT 670 680 690 700 710 720 DALESVAEDD TIEDDMLSYH EGLVLVPRGS ENTVTGPHIR GSRLRASAVG DEEVDLLLPG 730 740 750 760 770 780 TARLQIQQGV NMPARAELIP GREDKEKQIL HDCCDVVLYL GIIDILQEYN MTKKIEHAYK 790 800 810 SLHFDSLSIS AVDPTFYSQR FLEFIKKVFP QNNKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis thaliana putative phosphatidylinositol-4-phosphate 5-kinase." Lou Y. Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C." Mueller-Roeber B., Pical C. Plant Physiol. 130:22-46(2002) [PubMed: 12226484] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ810853 mRNA. Translation: CAH18644.1. AC015985 Genomic DNA. Translation: AAF23244.1. AY120742 mRNA. Translation: AAM53300.1.
IPI	IPI00523155.
RefSeq	NP_001030666.1. NP_187603.1.
UniGene	At.40019
3D structure databases
HSSP	HSSP built from PDB template 1BO1 based on UniProtKB P78356.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8L850. 1 interaction.
Genome annotation databases
GeneID	820151.
GenomeReviews	Gene locus AT3G09920 in contig BA000014_GR.
KEGG	ath:AT3G09920.
NMPDR	fig|3702.1.peg.13012.
Organism-specific databases
TAIR	At3g09920.
Phylogenomic databases
OMA	Q8L850. RRWSLEV.
Enzyme and pathway databases
BRENDA	2.7.1.68. 302.
Gene expression databases
GermOnline	AT3G09920. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003409. MORN. IPR017163. PIno-4-P-5_kinase_pln. IPR002498. PInositol-4-P-5-kinase_core. IPR016034. PInositol-4P-5-kinase_core_sub. [Graphical view]
PANTHER	PTHR23086. PIP5K. 1 hit.
Pfam	PF02493. MORN. 8 hits. PF01504. PIP5K. 1 hit. [Graphical view]
PIRSF	PIRSF037274. PIP5K_plant_prd. 1 hit.
SMART	SM00698. MORN. 8 hits. SM00330. PIPKc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI5K9_ARATH

Accession

Primary (citable) accession number: Q8L850
Secondary accession number(s): Q9SF93

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)