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Q8L850 (PI5K9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol 4-phosphate 5-kinase 9

Short name=AtPIP5K9
EC=2.7.1.68
Alternative name(s):
1-phosphatidylinositol 4-phosphate kinase 9
Diphosphoinositide kinase 9
PtdIns(4)P-5-kinase 9
Gene names
Name:PIP5K9
Ordered Locus Names:At3g09920
ORF Names:F8A24.2
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length815 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in sugar-mediated root development. Interaction with CINV1 induces repression of CINV1 activity and negative regulation of sugar-mediated root cell elongation. Ref.7

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Subunit structure

Interacts with CINV1. Ref.7

Subcellular location

Membrane; Peripheral membrane protein Probable. Nucleus.

Tissue specificity

Widely expressed. Ref.7

Induction

Transiently down-regulated by cold. Ref.7

Miscellaneous

The gain-of-function mutant pip5k9-d (T-DNA insertion line) has reduced primary root length (Ref.7).

Sequence similarities

Contains 8 MORN repeats.

Contains 1 PIPK domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CINV1Q9LQF26EBI-2008013,EBI-2008033

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 815815Phosphatidylinositol 4-phosphate 5-kinase 9
PRO_0000185481

Regions

Repeat58 – 8023MORN 1
Repeat81 – 10323MORN 2
Repeat104 – 12623MORN 3
Repeat127 – 14923MORN 4
Repeat150 – 17223MORN 5
Repeat173 – 19523MORN 6
Repeat196 – 21823MORN 7
Repeat219 – 24022MORN 8
Domain391 – 809419PIPK
Region769 – 79022Activation loop By similarity

Experimental info

Sequence conflict4821N → K in AAM53300. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q8L850 [UniParc].

Last modified October 25, 2005. Version 2.
Checksum: 3194F009D2C50130

FASTA81592,092
        10         20         30         40         50         60 
MSGLDVRGAV SFAERTKSVD ALTKKEILSA LNSGEVSETS EDARFRVREL VLPDGESYSG 

        70         80         90        100        110        120 
SLLGNVPEGP GKYIWSDGCV YDGEWRRGMR HGIGNMRWAS GASYDGEFSG GYMHGSGTYV 

       130        140        150        160        170        180 
DANKLTYKGR WRLNLKHGLG YQVYPNGDVF EGSWIQGLGE GPGKYTWANK NVYLGDMKGG 

       190        200        210        220        230        240 
KMSGKGTLTW VTGDSYEGSW LNGMMHGVGV YTWSDGGCYV GTWTRGLKDG KGSFYSAGTR 

       250        260        270        280        290        300 
VPVVQEFYLN ALRKRGVLPD MRRQNQVASS VNMENLRVGV NRNKLSKGSL INLEQSRNGR 

       310        320        330        340        350        360 
VSLERRWSLE VSIEKVIGHG YSDLSTAVLD SGSSVQYKAN IPILEREYMQ GVLISELVVN 

       370        380        390        400        410        420 
NGFSRTSRRA KRKHKRLVKE AKKPGEVVIK GHRSYDLMLS LQLGIRYTVG KITPIQRRQV 

       430        440        450        460        470        480 
RTADFGPRAS FWMTFPRAGS TMTPPHHSED FKWKDYCPMV FRNLREMFKI DAADYMMSIC 

       490        500        510        520        530        540 
GNDTLRELSS PGKSGSVFFL SQDDRFMIKT LRKSEVKVLL RMLPDYHHHV KTYENTLITK 

       550        560        570        580        590        600 
FFGLHRIKPS SGQKFRFVVM GNMFFTDLRI HRRFDLKGSS LGRSADKVEI DENTILKDLD 

       610        620        630        640        650        660 
LNYSFFLETS WREGLLRQLE IDSKFLEAQN IMDYSLLLGV HHRAPQHLRS QLVRSQSITT 

       670        680        690        700        710        720 
DALESVAEDD TIEDDMLSYH EGLVLVPRGS ENTVTGPHIR GSRLRASAVG DEEVDLLLPG 

       730        740        750        760        770        780 
TARLQIQQGV NMPARAELIP GREDKEKQIL HDCCDVVLYL GIIDILQEYN MTKKIEHAYK 

       790        800        810 
SLHFDSLSIS AVDPTFYSQR FLEFIKKVFP QNNKS 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis thaliana putative phosphatidylinositol-4-phosphate 5-kinase."
Lou Y.
Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
Mueller-Roeber B., Pical C.
Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[7]"PIP5K9, an Arabidopsis phosphatidylinositol monophosphate kinase, interacts with a cytosolic invertase to negatively regulate sugar-mediated root growth."
Lou Y., Gou J.Y., Xue H.W.
Plant Cell 19:163-181(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CINV1, TISSUE SPECIFICITY, INDUCTION BY COLD.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ810853 mRNA. Translation: CAH18644.1.
AC015985 Genomic DNA. Translation: AAF23244.1.
CP002686 Genomic DNA. Translation: AEE74832.1.
CP002686 Genomic DNA. Translation: AEE74833.1.
CP002686 Genomic DNA. Translation: AEE74834.1.
AY120742 mRNA. Translation: AAM53300.1.
AK229815 mRNA. Translation: BAF01646.1.
RefSeqNP_001030666.1. NM_001035589.2.
NP_001189852.1. NM_001202923.1.
NP_187603.1. NM_111827.2.
UniGeneAt.40019.

3D structure databases

ProteinModelPortalQ8L850.
SMRQ8L850. Positions 451-641.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid5485. 1 interaction.
IntActQ8L850. 1 interaction.

Proteomic databases

PaxDbQ8L850.
PRIDEQ8L850.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G09920.1; AT3G09920.1; AT3G09920.
AT3G09920.2; AT3G09920.2; AT3G09920.
AT3G09920.3; AT3G09920.3; AT3G09920.
GeneID820151.
KEGGath:AT3G09920.

Organism-specific databases

TAIRAT3G09920.

Phylogenomic databases

eggNOGCOG4642.
HOGENOMHOG000193875.
InParanoidQ8L850.
KOK00889.
OMAIQKREVR.
PhylomeDBQ8L850.

Enzyme and pathway databases

BioCycARA:AT3G09920-MONOMER.
ARA:GQT-1409-MONOMER.
ARA:GQT-2574-MONOMER.
BRENDA2.7.1.68. 399.

Gene expression databases

ArrayExpressQ8L850.
GenevestigatorQ8L850.

Family and domain databases

Gene3D3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
InterProIPR003409. MORN.
IPR017163. PIno-4-P-5_kinase_pln.
IPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERPTHR23086. PTHR23086. 1 hit.
PfamPF02493. MORN. 8 hits.
PF01504. PIP5K. 1 hit.
[Graphical view]
PIRSFPIRSF037274. PIP5K_plant_prd. 1 hit.
SMARTSM00698. MORN. 8 hits.
SM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEPS51455. PIPK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePI5K9_ARATH
AccessionPrimary (citable) accession number: Q8L850
Secondary accession number(s): Q0WMK5, Q9SF93
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: June 11, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names