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Protein

Alpha-L-fucosidase 2

Gene

FUC95A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes alpha-1,2-linked fucose. Also active on fucosylated xyloglucan oligosaccharides. No activity with 3-fucosyllactose, p-nitrophenyl-alpha-I-fucopyranoside, lacto-N-fucopentaose II, lacto-N-fucopentaose III or alpha 1,6-fucosylated chitopentaose. Involved in apoplastic xyloglucan metabolism.2 Publications

Catalytic activityi

An alpha-L-fucoside + H2O = L-fucose + an alcohol.1 Publication

Kineticsi

  1. KM=0.65 mM for 2-fucosyllactose1 Publication
  2. KM=1.5 mM for polymeric xyloglucan1 Publication

    pH dependencei

    Optimum pH is 5.0.1 Publication

    GO - Molecular functioni

    • 1,2-alpha-L-fucosidase activity Source: TAIR

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT4G34260-MONOMER.

    Protein family/group databases

    CAZyiGH95. Glycoside Hydrolase Family 95.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-L-fucosidase 2 (EC:3.2.1.51)
    Alternative name(s):
    Alpha-1,2-fucosidase 2
    Alpha-L-fucosidase 95A
    Short name:
    AtFuc95A
    Alpha-L-fucoside fucohydrolase 2
    Protein ALTERED XYLOGLUCAN 8
    Gene namesi
    Name:FUC95A
    Synonyms:AXY8
    Ordered Locus Names:At4g34260
    ORF Names:F10M10.30
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRiAT4G34260.

    Subcellular locationi

    GO - Cellular componenti

    • apoplast Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Apoplast, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype. Higher abundance of fucosylated oligosaccharides and presence of unusual oligosaccharides.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi511C → Y in axy8-4; Loss of activity. 1 Publication1
    Mutagenesisi562P → L in axy8-3; Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 27Sequence analysisAdd BLAST27
    ChainiPRO_000028987728 – 843Alpha-L-fucosidase 2Add BLAST816

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi62N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi253N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi365N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi605N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8L7W8.

    Expressioni

    Tissue specificityi

    Ubiquitous. Highest expression in vascular tissues, leaf trichomes, root elongation zone and emerging lateral roots.1 Publication

    Gene expression databases

    GenevisibleiQ8L7W8. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G34260.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L7W8.
    SMRiQ8L7W8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 95 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IHPJ. Eukaryota.
    ENOG410XPGV. LUCA.
    HOGENOMiHOG000161832.
    InParanoidiQ8L7W8.
    KOiK15923.
    OMAiDAGDPMW.
    OrthoDBiEOG0936034P.
    PhylomeDBiQ8L7W8.

    Family and domain databases

    Gene3Di2.70.98.50. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR016518. Alpha-L-fucosidase.
    IPR027414. GH_fam_N_dom.
    [Graphical view]
    PfamiPF14498. Glyco_hyd_65N_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF007663. UCP007663. 1 hit.
    SUPFAMiSSF48208. SSF48208. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8L7W8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAEKSSFFVH FSCLLLLLTI IITCGEGVRN PVRPRSSERR ALMDGQDLSR
    60 70 80 90 100
    PLKLTFGGPS RNWTDAIPIG NGRLGATIWG GVSSEILNIN EDTIWTGVPA
    110 120 130 140 150
    DYTNQKAPEA LAEVRRLVDE RNYAEATSEA VKLSGQPSDV YQIVGDLNLE
    160 170 180 190 200
    FDSSHRKYTQ ASYRRELDLE TAVAKVSYSV GAVDFSREFF ASNPDQVIIA
    210 220 230 240 250
    KIYASKPGSL SFKVSFDSEL HHHSETNPKA NQILMRGSCR PKRLPVNLKK
    260 270 280 290 300
    SINATNIPYD DHKGLQFASI LEVRVSNGGS VSSLGGKKLS VEKADWAVLL
    310 320 330 340 350
    LAASSNFDGP FTMPVDSKID PAKECVNRIS SVQKYSYSDL YARHLGDYQK
    360 370 380 390 400
    LFNRVSLHLS GSSTNETVQQ ATSTAERVRS FKTDQDPSLV ELLFQYGRYL
    410 420 430 440 450
    LISSSRPGTQ VANLQGIWNR DIQPPWDGAP HLNINLQMNY WHSLPGNIRE
    460 470 480 490 500
    CQEPLFDYMS ALAINGRKTA QVNYGASGWV AHQVSDIWAK TSPDRGEAVW
    510 520 530 540 550
    ALWPMGGAWL CTHAWEHYTY TMDKEFLKKK GYPLLEGCTS FLLDWLIKGK
    560 570 580 590 600
    DGFLQTNPST SPEHMFTAPI GKPASVSYSS TMDIAIIKEV FADIVSASEI
    610 620 630 640 650
    LGKTNDTLIG KVIAAQAKLP PTRISKDGSI REWAEDFEDP EVHHRHVSHL
    660 670 680 690 700
    FGLFPGHTIT VEKSPELAKA VEATLKKRGE EGPGWSTTWK AALWARLHNS
    710 720 730 740 750
    EHAYRMVTHI FDLVDPLNER NYEGGLYSNM FTAHPPFQID ANFGFAAAVA
    760 770 780 790 800
    EMLVQSTTKD LYLLPALPAD KWPNGIVNGL RARGGVTVSI KWMEGNLVEF
    810 820 830 840
    GLWSEQIVST RIVYRGISAA AELLPGKVFT FDKDLRCIRT DKL
    Length:843
    Mass (Da):93,725
    Last modified:October 1, 2002 - v1
    Checksum:i2EF1315DF98A1365
    GO

    Sequence cautioni

    The sequence CAB36703 differs from that shown. Reason: Erroneous gene model prediction.Curated
    The sequence CAB80143 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL035521 Genomic DNA. Translation: CAB36703.1. Sequence problems.
    AL161585 Genomic DNA. Translation: CAB80143.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE86349.1.
    AY125494 mRNA. Translation: AAM78086.1.
    BT002722 mRNA. Translation: AAO11638.1.
    PIRiT04772.
    RefSeqiNP_195152.2. NM_119590.4.
    UniGeneiAt.45833.

    Genome annotation databases

    EnsemblPlantsiAT4G34260.1; AT4G34260.1; AT4G34260.
    GeneIDi829575.
    GrameneiAT4G34260.1; AT4G34260.1; AT4G34260.
    KEGGiath:AT4G34260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL035521 Genomic DNA. Translation: CAB36703.1. Sequence problems.
    AL161585 Genomic DNA. Translation: CAB80143.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE86349.1.
    AY125494 mRNA. Translation: AAM78086.1.
    BT002722 mRNA. Translation: AAO11638.1.
    PIRiT04772.
    RefSeqiNP_195152.2. NM_119590.4.
    UniGeneiAt.45833.

    3D structure databases

    ProteinModelPortaliQ8L7W8.
    SMRiQ8L7W8.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT4G34260.1.

    Protein family/group databases

    CAZyiGH95. Glycoside Hydrolase Family 95.

    Proteomic databases

    PaxDbiQ8L7W8.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G34260.1; AT4G34260.1; AT4G34260.
    GeneIDi829575.
    GrameneiAT4G34260.1; AT4G34260.1; AT4G34260.
    KEGGiath:AT4G34260.

    Organism-specific databases

    TAIRiAT4G34260.

    Phylogenomic databases

    eggNOGiENOG410IHPJ. Eukaryota.
    ENOG410XPGV. LUCA.
    HOGENOMiHOG000161832.
    InParanoidiQ8L7W8.
    KOiK15923.
    OMAiDAGDPMW.
    OrthoDBiEOG0936034P.
    PhylomeDBiQ8L7W8.

    Enzyme and pathway databases

    BioCyciARA:AT4G34260-MONOMER.

    Miscellaneous databases

    PROiQ8L7W8.

    Gene expression databases

    GenevisibleiQ8L7W8. AT.

    Family and domain databases

    Gene3Di2.70.98.50. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR016518. Alpha-L-fucosidase.
    IPR027414. GH_fam_N_dom.
    [Graphical view]
    PfamiPF14498. Glyco_hyd_65N_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF007663. UCP007663. 1 hit.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiFUCO2_ARATH
    AccessioniPrimary (citable) accession number: Q8L7W8
    Secondary accession number(s): Q9SYZ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: October 1, 2002
    Last modified: November 30, 2016
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.