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Q8L7W8

- FUCO2_ARATH

UniProt

Q8L7W8 - FUCO2_ARATH

Protein

Alpha-L-fucosidase 2

Gene

FUC95A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Hydrolyzes alpha-1,2-linked fucose. Also active on fucosylated xyloglucan oligosaccharides. No activity with 3-fucosyllactose, p-nitrophenyl-alpha-I-fucopyranoside, lacto-N-fucopentaose II, lacto-N-fucopentaose III or alpha 1,6-fucosylated chitopentaose. Involved in apoplastic xyloglucan metabolism.2 Publications

    Catalytic activityi

    An alpha-L-fucoside + H2O = L-fucose + an alcohol.1 Publication

    Kineticsi

    1. KM=0.65 mM for 2-fucosyllactose1 Publication
    2. KM=1.5 mM for polymeric xyloglucan1 Publication

    pH dependencei

    Optimum pH is 5.0.1 Publication

    GO - Molecular functioni

    1. 1,2-alpha-L-fucosidase activity Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT4G34260-MONOMER.

    Protein family/group databases

    CAZyiGH95. Glycoside Hydrolase Family 95.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-L-fucosidase 2 (EC:3.2.1.51)
    Alternative name(s):
    Alpha-1,2-fucosidase 2
    Alpha-L-fucosidase 95A
    Short name:
    AtFuc95A
    Alpha-L-fucoside fucohydrolase 2
    Protein ALTERED XYLOGLUCAN 8
    Gene namesi
    Name:FUC95A
    Synonyms:AXY8
    Ordered Locus Names:At4g34260
    ORF Names:F10M10.30
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G34260.

    Subcellular locationi

    Secretedextracellular spaceapoplast 1 Publication

    GO - Cellular componenti

    1. apoplast Source: TAIR

    Keywords - Cellular componenti

    Apoplast, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype. Higher abundance of fucosylated oligosaccharides and presence of unusual oligosaccharides.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi511 – 5111C → Y in axy8-4; Loss of activity. 1 Publication
    Mutagenesisi562 – 5621P → L in axy8-3; Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 843816Alpha-L-fucosidase 2PRO_0000289877Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi62 – 621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi605 – 6051N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8L7W8.
    PRIDEiQ8L7W8.

    Expressioni

    Tissue specificityi

    Ubiquitous. Highest expression in vascular tissues, leaf trichomes, root elongation zone and emerging lateral roots.1 Publication

    Gene expression databases

    GenevestigatoriQ8L7W8.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G34260.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L7W8.
    SMRiQ8L7W8. Positions 56-806.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 95 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG04067.
    HOGENOMiHOG000161832.
    InParanoidiQ8L7W8.
    KOiK15923.
    OMAiCKYTINI.
    PhylomeDBiQ8L7W8.

    Family and domain databases

    Gene3Di2.70.98.50. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR016518. Alpha-L-fucosidase.
    IPR027414. GH_fam_N_dom.
    [Graphical view]
    PfamiPF14498. Glyco_hyd_65N_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF007663. UCP007663. 1 hit.
    SUPFAMiSSF48208. SSF48208. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8L7W8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEKSSFFVH FSCLLLLLTI IITCGEGVRN PVRPRSSERR ALMDGQDLSR    50
    PLKLTFGGPS RNWTDAIPIG NGRLGATIWG GVSSEILNIN EDTIWTGVPA 100
    DYTNQKAPEA LAEVRRLVDE RNYAEATSEA VKLSGQPSDV YQIVGDLNLE 150
    FDSSHRKYTQ ASYRRELDLE TAVAKVSYSV GAVDFSREFF ASNPDQVIIA 200
    KIYASKPGSL SFKVSFDSEL HHHSETNPKA NQILMRGSCR PKRLPVNLKK 250
    SINATNIPYD DHKGLQFASI LEVRVSNGGS VSSLGGKKLS VEKADWAVLL 300
    LAASSNFDGP FTMPVDSKID PAKECVNRIS SVQKYSYSDL YARHLGDYQK 350
    LFNRVSLHLS GSSTNETVQQ ATSTAERVRS FKTDQDPSLV ELLFQYGRYL 400
    LISSSRPGTQ VANLQGIWNR DIQPPWDGAP HLNINLQMNY WHSLPGNIRE 450
    CQEPLFDYMS ALAINGRKTA QVNYGASGWV AHQVSDIWAK TSPDRGEAVW 500
    ALWPMGGAWL CTHAWEHYTY TMDKEFLKKK GYPLLEGCTS FLLDWLIKGK 550
    DGFLQTNPST SPEHMFTAPI GKPASVSYSS TMDIAIIKEV FADIVSASEI 600
    LGKTNDTLIG KVIAAQAKLP PTRISKDGSI REWAEDFEDP EVHHRHVSHL 650
    FGLFPGHTIT VEKSPELAKA VEATLKKRGE EGPGWSTTWK AALWARLHNS 700
    EHAYRMVTHI FDLVDPLNER NYEGGLYSNM FTAHPPFQID ANFGFAAAVA 750
    EMLVQSTTKD LYLLPALPAD KWPNGIVNGL RARGGVTVSI KWMEGNLVEF 800
    GLWSEQIVST RIVYRGISAA AELLPGKVFT FDKDLRCIRT DKL 843
    Length:843
    Mass (Da):93,725
    Last modified:October 1, 2002 - v1
    Checksum:i2EF1315DF98A1365
    GO

    Sequence cautioni

    The sequence CAB36703.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB80143.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL035521 Genomic DNA. Translation: CAB36703.1. Sequence problems.
    AL161585 Genomic DNA. Translation: CAB80143.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE86349.1.
    AY125494 mRNA. Translation: AAM78086.1.
    BT002722 mRNA. Translation: AAO11638.1.
    PIRiT04772.
    RefSeqiNP_195152.2. NM_119590.3.
    UniGeneiAt.45833.

    Genome annotation databases

    EnsemblPlantsiAT4G34260.1; AT4G34260.1; AT4G34260.
    GeneIDi829575.
    KEGGiath:AT4G34260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL035521 Genomic DNA. Translation: CAB36703.1 . Sequence problems.
    AL161585 Genomic DNA. Translation: CAB80143.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE86349.1 .
    AY125494 mRNA. Translation: AAM78086.1 .
    BT002722 mRNA. Translation: AAO11638.1 .
    PIRi T04772.
    RefSeqi NP_195152.2. NM_119590.3.
    UniGenei At.45833.

    3D structure databases

    ProteinModelPortali Q8L7W8.
    SMRi Q8L7W8. Positions 56-806.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT4G34260.1-P.

    Protein family/group databases

    CAZyi GH95. Glycoside Hydrolase Family 95.

    Proteomic databases

    PaxDbi Q8L7W8.
    PRIDEi Q8L7W8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G34260.1 ; AT4G34260.1 ; AT4G34260 .
    GeneIDi 829575.
    KEGGi ath:AT4G34260.

    Organism-specific databases

    TAIRi AT4G34260.

    Phylogenomic databases

    eggNOGi NOG04067.
    HOGENOMi HOG000161832.
    InParanoidi Q8L7W8.
    KOi K15923.
    OMAi CKYTINI.
    PhylomeDBi Q8L7W8.

    Enzyme and pathway databases

    BioCyci ARA:AT4G34260-MONOMER.

    Gene expression databases

    Genevestigatori Q8L7W8.

    Family and domain databases

    Gene3Di 2.70.98.50. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR016518. Alpha-L-fucosidase.
    IPR027414. GH_fam_N_dom.
    [Graphical view ]
    Pfami PF14498. Glyco_hyd_65N_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF007663. UCP007663. 1 hit.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Molecular cloning and characterization of Bifidobacterium bifidum 1,2-alpha-L-fucosidase (AfcA), a novel inverting glycosidase (glycoside hydrolase family 95)."
      Katayama T., Sakuma A., Kimura T., Makimura Y., Hiratake J., Sakata K., Yamanoi T., Kumagai H., Yamamoto K.
      J. Bacteriol. 186:4885-4893(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    5. "Identification of an Arabidopsis gene encoding a GH95 alpha1,2-fucosidase active on xyloglucan oligo- and polysaccharides."
      Leonard R., Pabst M., Bondili J.S., Chambat G., Veit C., Strasser R., Altmann F.
      Phytochemistry 69:1983-1988(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    6. "AXY8 encodes an alpha-fucosidase, underscoring the importance of apoplastic metabolism on the fine structure of Arabidopsis cell wall polysaccharides."
      Gunl M., Neumetzler L., Kraemer F., de Souza A., Schultink A., Pena M., York W.S., Pauly M.
      Plant Cell 23:4025-4040(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF CYS-511 AND PRO-562, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiFUCO2_ARATH
    AccessioniPrimary (citable) accession number: Q8L7W8
    Secondary accession number(s): Q9SYZ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3