Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-L-fucosidase 2

Gene

FUC95A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes alpha-1,2-linked fucose. Also active on fucosylated xyloglucan oligosaccharides. No activity with 3-fucosyllactose, p-nitrophenyl-alpha-I-fucopyranoside, lacto-N-fucopentaose II, lacto-N-fucopentaose III or alpha 1,6-fucosylated chitopentaose. Involved in apoplastic xyloglucan metabolism.2 Publications

Catalytic activityi

An alpha-L-fucoside + H2O = L-fucose + an alcohol.1 Publication

Kineticsi

  1. KM=0.65 mM for 2-fucosyllactose1 Publication
  2. KM=1.5 mM for polymeric xyloglucan1 Publication

    pH dependencei

    Optimum pH is 5.0.1 Publication

    GO - Molecular functioni

    • 1,2-alpha-L-fucosidase activity Source: TAIR

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT4G34260-MONOMER

    Protein family/group databases

    CAZyiGH95 Glycoside Hydrolase Family 95

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-L-fucosidase 2 (EC:3.2.1.51)
    Alternative name(s):
    Alpha-1,2-fucosidase 2
    Alpha-L-fucosidase 95A
    Short name:
    AtFuc95A
    Alpha-L-fucoside fucohydrolase 2
    Protein ALTERED XYLOGLUCAN 8
    Gene namesi
    Name:FUC95A
    Synonyms:AXY8
    Ordered Locus Names:At4g34260
    ORF Names:F10M10.30
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    AraportiAT4G34260
    TAIRilocus:2116154 AT4G34260

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Apoplast, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype. Higher abundance of fucosylated oligosaccharides and presence of unusual oligosaccharides.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi511C → Y in axy8-4; Loss of activity. 1 Publication1
    Mutagenesisi562P → L in axy8-3; Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 27Sequence analysisAdd BLAST27
    ChainiPRO_000028987728 – 843Alpha-L-fucosidase 2Add BLAST816

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi62N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi253N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi365N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi605N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8L7W8
    PRIDEiQ8L7W8

    Expressioni

    Tissue specificityi

    Ubiquitous. Highest expression in vascular tissues, leaf trichomes, root elongation zone and emerging lateral roots.1 Publication

    Gene expression databases

    ExpressionAtlasiQ8L7W8 baseline and differential
    GenevisibleiQ8L7W8 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G34260.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L7W8
    SMRiQ8L7W8
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 95 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IHPJ Eukaryota
    ENOG410XPGV LUCA
    HOGENOMiHOG000161832
    InParanoidiQ8L7W8
    KOiK15923
    OMAiMGAAWLC
    OrthoDBiEOG0936034P
    PhylomeDBiQ8L7W8

    Family and domain databases

    Gene3Di1.50.10.10, 3 hits
    2.60.40.1180, 2 hits
    InterProiView protein in InterPro
    IPR008928 6-hairpin_glycosidase_sf
    IPR012341 6hp_glycosidase-like_sf
    IPR016518 Alpha-L-fucosidase
    IPR027414 GH95_N_dom
    IPR013780 Glyco_hydro_b
    PfamiView protein in Pfam
    PF14498 Glyco_hyd_65N_2, 1 hit
    PIRSFiPIRSF007663 UCP007663, 1 hit
    SUPFAMiSSF48208 SSF48208, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8L7W8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAEKSSFFVH FSCLLLLLTI IITCGEGVRN PVRPRSSERR ALMDGQDLSR
    60 70 80 90 100
    PLKLTFGGPS RNWTDAIPIG NGRLGATIWG GVSSEILNIN EDTIWTGVPA
    110 120 130 140 150
    DYTNQKAPEA LAEVRRLVDE RNYAEATSEA VKLSGQPSDV YQIVGDLNLE
    160 170 180 190 200
    FDSSHRKYTQ ASYRRELDLE TAVAKVSYSV GAVDFSREFF ASNPDQVIIA
    210 220 230 240 250
    KIYASKPGSL SFKVSFDSEL HHHSETNPKA NQILMRGSCR PKRLPVNLKK
    260 270 280 290 300
    SINATNIPYD DHKGLQFASI LEVRVSNGGS VSSLGGKKLS VEKADWAVLL
    310 320 330 340 350
    LAASSNFDGP FTMPVDSKID PAKECVNRIS SVQKYSYSDL YARHLGDYQK
    360 370 380 390 400
    LFNRVSLHLS GSSTNETVQQ ATSTAERVRS FKTDQDPSLV ELLFQYGRYL
    410 420 430 440 450
    LISSSRPGTQ VANLQGIWNR DIQPPWDGAP HLNINLQMNY WHSLPGNIRE
    460 470 480 490 500
    CQEPLFDYMS ALAINGRKTA QVNYGASGWV AHQVSDIWAK TSPDRGEAVW
    510 520 530 540 550
    ALWPMGGAWL CTHAWEHYTY TMDKEFLKKK GYPLLEGCTS FLLDWLIKGK
    560 570 580 590 600
    DGFLQTNPST SPEHMFTAPI GKPASVSYSS TMDIAIIKEV FADIVSASEI
    610 620 630 640 650
    LGKTNDTLIG KVIAAQAKLP PTRISKDGSI REWAEDFEDP EVHHRHVSHL
    660 670 680 690 700
    FGLFPGHTIT VEKSPELAKA VEATLKKRGE EGPGWSTTWK AALWARLHNS
    710 720 730 740 750
    EHAYRMVTHI FDLVDPLNER NYEGGLYSNM FTAHPPFQID ANFGFAAAVA
    760 770 780 790 800
    EMLVQSTTKD LYLLPALPAD KWPNGIVNGL RARGGVTVSI KWMEGNLVEF
    810 820 830 840
    GLWSEQIVST RIVYRGISAA AELLPGKVFT FDKDLRCIRT DKL
    Length:843
    Mass (Da):93,725
    Last modified:October 1, 2002 - v1
    Checksum:i2EF1315DF98A1365
    GO

    Sequence cautioni

    The sequence CAB36703 differs from that shown. Reason: Erroneous gene model prediction.Curated
    The sequence CAB80143 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL035521 Genomic DNA Translation: CAB36703.1 Sequence problems.
    AL161585 Genomic DNA Translation: CAB80143.1 Sequence problems.
    CP002687 Genomic DNA Translation: AEE86349.1
    AY125494 mRNA Translation: AAM78086.1
    BT002722 mRNA Translation: AAO11638.1
    PIRiT04772
    RefSeqiNP_195152.2, NM_119590.4
    UniGeneiAt.45833

    Genome annotation databases

    EnsemblPlantsiAT4G34260.1; AT4G34260.1; AT4G34260
    GeneIDi829575
    GrameneiAT4G34260.1; AT4G34260.1; AT4G34260
    KEGGiath:AT4G34260

    Similar proteinsi

    Entry informationi

    Entry nameiFUCO2_ARATH
    AccessioniPrimary (citable) accession number: Q8L7W8
    Secondary accession number(s): Q9SYZ1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: October 1, 2002
    Last modified: May 23, 2018
    This is version 103 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Cookie policy

    We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health