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Q8L7W8 (FUCO2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-L-fucosidase 2

EC=3.2.1.51
Alternative name(s):
Alpha-1,2-fucosidase 2
Alpha-L-fucoside fucohydrolase 2
Gene names
Ordered Locus Names:At4g34260
ORF Names:F10M10.30
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length843 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes alpha-1,2-linked fucose. Also active on fucosylated xyloglucan oligosaccharides. Ref.5

Catalytic activity

An alpha-L-fucoside + H2O = L-fucose + an alcohol.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 95 family.

Sequence caution

The sequence CAB36703.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB80143.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentapoplast

Inferred from direct assay PubMed 22080600. Source: TAIR

   Molecular_function1,2-alpha-L-fucosidase activity

Inferred from direct assay PubMed 18495185. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 843816Alpha-L-fucosidase 2
PRO_0000289877

Amino acid modifications

Glycosylation621N-linked (GlcNAc...) Potential
Glycosylation2531N-linked (GlcNAc...) Potential
Glycosylation3651N-linked (GlcNAc...) Potential
Glycosylation6051N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8L7W8 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 2EF1315DF98A1365

FASTA84393,725
        10         20         30         40         50         60 
MAEKSSFFVH FSCLLLLLTI IITCGEGVRN PVRPRSSERR ALMDGQDLSR PLKLTFGGPS 

        70         80         90        100        110        120 
RNWTDAIPIG NGRLGATIWG GVSSEILNIN EDTIWTGVPA DYTNQKAPEA LAEVRRLVDE 

       130        140        150        160        170        180 
RNYAEATSEA VKLSGQPSDV YQIVGDLNLE FDSSHRKYTQ ASYRRELDLE TAVAKVSYSV 

       190        200        210        220        230        240 
GAVDFSREFF ASNPDQVIIA KIYASKPGSL SFKVSFDSEL HHHSETNPKA NQILMRGSCR 

       250        260        270        280        290        300 
PKRLPVNLKK SINATNIPYD DHKGLQFASI LEVRVSNGGS VSSLGGKKLS VEKADWAVLL 

       310        320        330        340        350        360 
LAASSNFDGP FTMPVDSKID PAKECVNRIS SVQKYSYSDL YARHLGDYQK LFNRVSLHLS 

       370        380        390        400        410        420 
GSSTNETVQQ ATSTAERVRS FKTDQDPSLV ELLFQYGRYL LISSSRPGTQ VANLQGIWNR 

       430        440        450        460        470        480 
DIQPPWDGAP HLNINLQMNY WHSLPGNIRE CQEPLFDYMS ALAINGRKTA QVNYGASGWV 

       490        500        510        520        530        540 
AHQVSDIWAK TSPDRGEAVW ALWPMGGAWL CTHAWEHYTY TMDKEFLKKK GYPLLEGCTS 

       550        560        570        580        590        600 
FLLDWLIKGK DGFLQTNPST SPEHMFTAPI GKPASVSYSS TMDIAIIKEV FADIVSASEI 

       610        620        630        640        650        660 
LGKTNDTLIG KVIAAQAKLP PTRISKDGSI REWAEDFEDP EVHHRHVSHL FGLFPGHTIT 

       670        680        690        700        710        720 
VEKSPELAKA VEATLKKRGE EGPGWSTTWK AALWARLHNS EHAYRMVTHI FDLVDPLNER 

       730        740        750        760        770        780 
NYEGGLYSNM FTAHPPFQID ANFGFAAAVA EMLVQSTTKD LYLLPALPAD KWPNGIVNGL 

       790        800        810        820        830        840 
RARGGVTVSI KWMEGNLVEF GLWSEQIVST RIVYRGISAA AELLPGKVFT FDKDLRCIRT 


DKL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Molecular cloning and characterization of Bifidobacterium bifidum 1,2-alpha-L-fucosidase (AfcA), a novel inverting glycosidase (glycoside hydrolase family 95)."
Katayama T., Sakuma A., Kimura T., Makimura Y., Hiratake J., Sakata K., Yamanoi T., Kumagai H., Yamamoto K.
J. Bacteriol. 186:4885-4893(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[5]"Cloning and expression of an A. thaliana alpha1,2-fucosidase active against fucosylated xyloglucan oligosaccharides."
Leonard R., Bondili J.S., Altmann F.
Unpublished observations (MAY-2007)
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL035521 Genomic DNA. Translation: CAB36703.1. Sequence problems.
AL161585 Genomic DNA. Translation: CAB80143.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86349.1.
AY125494 mRNA. Translation: AAM78086.1.
BT002722 mRNA. Translation: AAO11638.1.
PIRT04772.
RefSeqNP_195152.2. NM_119590.3.
UniGeneAt.45833.

3D structure databases

ProteinModelPortalQ8L7W8.
SMRQ8L7W8. Positions 52-817.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT4G34260.1-P.

Protein family/group databases

CAZyGH95. Glycoside Hydrolase Family 95.

Proteomic databases

PaxDbQ8L7W8.
PRIDEQ8L7W8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G34260.1; AT4G34260.1; AT4G34260.
GeneID829575.
KEGGath:AT4G34260.

Organism-specific databases

TAIRAT4G34260.

Phylogenomic databases

eggNOGNOG04067.
HOGENOMHOG000161832.
InParanoidQ8L7W8.
KOK15923.
OMAWAGGPYD.
PhylomeDBQ8L7W8.
ProtClustDBCLSN2680255.

Enzyme and pathway databases

BioCycARA:AT4G34260-MONOMER.

Gene expression databases

GenevestigatorQ8L7W8.

Family and domain databases

Gene3D2.70.98.50. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR016518. Alpha-L-fucosidase.
IPR027414. GH_fam_N_dom.
[Graphical view]
PfamPF14498. Glyco_hyd_65N_2. 1 hit.
[Graphical view]
PIRSFPIRSF007663. UCP007663. 1 hit.
SUPFAMSSF48208. SSF48208. 1 hit.
ProtoNetSearch...

Entry information

Entry nameFUCO2_ARATH
AccessionPrimary (citable) accession number: Q8L7W8
Secondary accession number(s): Q9SYZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names