Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-L-fucosidase 2

Gene

FUC95A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes alpha-1,2-linked fucose. Also active on fucosylated xyloglucan oligosaccharides. No activity with 3-fucosyllactose, p-nitrophenyl-alpha-I-fucopyranoside, lacto-N-fucopentaose II, lacto-N-fucopentaose III or alpha 1,6-fucosylated chitopentaose. Involved in apoplastic xyloglucan metabolism.2 Publications

Catalytic activityi

An alpha-L-fucoside + H2O = L-fucose + an alcohol.1 Publication

Kineticsi

  1. KM=0.65 mM for 2-fucosyllactose1 Publication
  2. KM=1.5 mM for polymeric xyloglucan1 Publication

    pH dependencei

    Optimum pH is 5.0.1 Publication

    GO - Molecular functioni

    • 1,2-alpha-L-fucosidase activity Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT4G34260-MONOMER.

    Protein family/group databases

    CAZyiGH95. Glycoside Hydrolase Family 95.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-L-fucosidase 2 (EC:3.2.1.51)
    Alternative name(s):
    Alpha-1,2-fucosidase 2
    Alpha-L-fucosidase 95A
    Short name:
    AtFuc95A
    Alpha-L-fucoside fucohydrolase 2
    Protein ALTERED XYLOGLUCAN 8
    Gene namesi
    Name:FUC95A
    Synonyms:AXY8
    Ordered Locus Names:At4g34260
    ORF Names:F10M10.30
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRiAT4G34260.

    Subcellular locationi

    GO - Cellular componenti

    • apoplast Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Apoplast, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype. Higher abundance of fucosylated oligosaccharides and presence of unusual oligosaccharides.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi511 – 5111C → Y in axy8-4; Loss of activity. 1 Publication
    Mutagenesisi562 – 5621P → L in axy8-3; Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 843816Alpha-L-fucosidase 2PRO_0000289877Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi62 – 621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi605 – 6051N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8L7W8.
    PRIDEiQ8L7W8.

    Expressioni

    Tissue specificityi

    Ubiquitous. Highest expression in vascular tissues, leaf trichomes, root elongation zone and emerging lateral roots.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G34260.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L7W8.
    SMRiQ8L7W8. Positions 56-806.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 95 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG04067.
    HOGENOMiHOG000161832.
    InParanoidiQ8L7W8.
    KOiK15923.
    OMAiDAGDPMW.
    PhylomeDBiQ8L7W8.

    Family and domain databases

    Gene3Di2.70.98.50. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR016518. Alpha-L-fucosidase.
    IPR027414. GH_fam_N_dom.
    [Graphical view]
    PfamiPF14498. Glyco_hyd_65N_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF007663. UCP007663. 1 hit.
    SUPFAMiSSF48208. SSF48208. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8L7W8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAEKSSFFVH FSCLLLLLTI IITCGEGVRN PVRPRSSERR ALMDGQDLSR
    60 70 80 90 100
    PLKLTFGGPS RNWTDAIPIG NGRLGATIWG GVSSEILNIN EDTIWTGVPA
    110 120 130 140 150
    DYTNQKAPEA LAEVRRLVDE RNYAEATSEA VKLSGQPSDV YQIVGDLNLE
    160 170 180 190 200
    FDSSHRKYTQ ASYRRELDLE TAVAKVSYSV GAVDFSREFF ASNPDQVIIA
    210 220 230 240 250
    KIYASKPGSL SFKVSFDSEL HHHSETNPKA NQILMRGSCR PKRLPVNLKK
    260 270 280 290 300
    SINATNIPYD DHKGLQFASI LEVRVSNGGS VSSLGGKKLS VEKADWAVLL
    310 320 330 340 350
    LAASSNFDGP FTMPVDSKID PAKECVNRIS SVQKYSYSDL YARHLGDYQK
    360 370 380 390 400
    LFNRVSLHLS GSSTNETVQQ ATSTAERVRS FKTDQDPSLV ELLFQYGRYL
    410 420 430 440 450
    LISSSRPGTQ VANLQGIWNR DIQPPWDGAP HLNINLQMNY WHSLPGNIRE
    460 470 480 490 500
    CQEPLFDYMS ALAINGRKTA QVNYGASGWV AHQVSDIWAK TSPDRGEAVW
    510 520 530 540 550
    ALWPMGGAWL CTHAWEHYTY TMDKEFLKKK GYPLLEGCTS FLLDWLIKGK
    560 570 580 590 600
    DGFLQTNPST SPEHMFTAPI GKPASVSYSS TMDIAIIKEV FADIVSASEI
    610 620 630 640 650
    LGKTNDTLIG KVIAAQAKLP PTRISKDGSI REWAEDFEDP EVHHRHVSHL
    660 670 680 690 700
    FGLFPGHTIT VEKSPELAKA VEATLKKRGE EGPGWSTTWK AALWARLHNS
    710 720 730 740 750
    EHAYRMVTHI FDLVDPLNER NYEGGLYSNM FTAHPPFQID ANFGFAAAVA
    760 770 780 790 800
    EMLVQSTTKD LYLLPALPAD KWPNGIVNGL RARGGVTVSI KWMEGNLVEF
    810 820 830 840
    GLWSEQIVST RIVYRGISAA AELLPGKVFT FDKDLRCIRT DKL
    Length:843
    Mass (Da):93,725
    Last modified:October 1, 2002 - v1
    Checksum:i2EF1315DF98A1365
    GO

    Sequence cautioni

    The sequence CAB36703.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
    The sequence CAB80143.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL035521 Genomic DNA. Translation: CAB36703.1. Sequence problems.
    AL161585 Genomic DNA. Translation: CAB80143.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE86349.1.
    AY125494 mRNA. Translation: AAM78086.1.
    BT002722 mRNA. Translation: AAO11638.1.
    PIRiT04772.
    RefSeqiNP_195152.2. NM_119590.3.
    UniGeneiAt.45833.

    Genome annotation databases

    EnsemblPlantsiAT4G34260.1; AT4G34260.1; AT4G34260.
    GeneIDi829575.
    KEGGiath:AT4G34260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL035521 Genomic DNA. Translation: CAB36703.1. Sequence problems.
    AL161585 Genomic DNA. Translation: CAB80143.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE86349.1.
    AY125494 mRNA. Translation: AAM78086.1.
    BT002722 mRNA. Translation: AAO11638.1.
    PIRiT04772.
    RefSeqiNP_195152.2. NM_119590.3.
    UniGeneiAt.45833.

    3D structure databases

    ProteinModelPortaliQ8L7W8.
    SMRiQ8L7W8. Positions 56-806.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT4G34260.1.

    Protein family/group databases

    CAZyiGH95. Glycoside Hydrolase Family 95.

    Proteomic databases

    PaxDbiQ8L7W8.
    PRIDEiQ8L7W8.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G34260.1; AT4G34260.1; AT4G34260.
    GeneIDi829575.
    KEGGiath:AT4G34260.

    Organism-specific databases

    TAIRiAT4G34260.

    Phylogenomic databases

    eggNOGiNOG04067.
    HOGENOMiHOG000161832.
    InParanoidiQ8L7W8.
    KOiK15923.
    OMAiDAGDPMW.
    PhylomeDBiQ8L7W8.

    Enzyme and pathway databases

    BioCyciARA:AT4G34260-MONOMER.

    Miscellaneous databases

    PROiQ8L7W8.

    Family and domain databases

    Gene3Di2.70.98.50. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR016518. Alpha-L-fucosidase.
    IPR027414. GH_fam_N_dom.
    [Graphical view]
    PfamiPF14498. Glyco_hyd_65N_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF007663. UCP007663. 1 hit.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Molecular cloning and characterization of Bifidobacterium bifidum 1,2-alpha-L-fucosidase (AfcA), a novel inverting glycosidase (glycoside hydrolase family 95)."
      Katayama T., Sakuma A., Kimura T., Makimura Y., Hiratake J., Sakata K., Yamanoi T., Kumagai H., Yamamoto K.
      J. Bacteriol. 186:4885-4893(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    5. "Identification of an Arabidopsis gene encoding a GH95 alpha1,2-fucosidase active on xyloglucan oligo- and polysaccharides."
      Leonard R., Pabst M., Bondili J.S., Chambat G., Veit C., Strasser R., Altmann F.
      Phytochemistry 69:1983-1988(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    6. "AXY8 encodes an alpha-fucosidase, underscoring the importance of apoplastic metabolism on the fine structure of Arabidopsis cell wall polysaccharides."
      Gunl M., Neumetzler L., Kraemer F., de Souza A., Schultink A., Pena M., York W.S., Pauly M.
      Plant Cell 23:4025-4040(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF CYS-511 AND PRO-562, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiFUCO2_ARATH
    AccessioniPrimary (citable) accession number: Q8L7W8
    Secondary accession number(s): Q9SYZ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: October 1, 2002
    Last modified: July 22, 2015
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.