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Q8L7U5

- BSL1_ARATH

UniProt

Q8L7U5 - BSL1_ARATH

Protein

Serine/threonine-protein phosphatase BSL1

Gene

BSL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (03 May 2011)
      Previous versions | rss
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    Functioni

    Phosphatase involved in elongation process, probably by acting as a regulator of brassinolide signaling.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi584 – 5841Manganese 1By similarity
    Metal bindingi586 – 5861Manganese 1By similarity
    Metal bindingi618 – 6181Manganese 1By similarity
    Metal bindingi618 – 6181Manganese 2By similarity
    Metal bindingi650 – 6501Manganese 2By similarity
    Active sitei651 – 6511Proton donorBy similarity
    Metal bindingi703 – 7031Manganese 2By similarity
    Metal bindingi782 – 7821Manganese 2By similarity

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. manganese ion binding Source: InterPro
    3. phosphoprotein phosphatase activity Source: UniProtKB-KW
    4. protein binding Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT4G03080-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase BSL1 (EC:3.1.3.16)
    Alternative name(s):
    BSU1-like protein 1
    Gene namesi
    Name:BSL1
    Ordered Locus Names:At4g03080
    ORF Names:T4I9.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G03080.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 881881Serine/threonine-protein phosphatase BSL1PRO_0000058905Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei491 – 4911Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8L7U5.
    PRIDEiQ8L7U5.

    Expressioni

    Tissue specificityi

    Expressed in mature cauline leaves and at the tip of influorescence, including flowers. Expressed at lower level in young tissues relative to older ones.1 Publication

    Gene expression databases

    GenevestigatoriQ8L7U5.

    Interactioni

    Protein-protein interaction databases

    BioGridi13388. 1 interaction.
    IntActiQ8L7U5. 1 interaction.
    STRINGi3702.AT4G03080.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L7U5.
    SMRiQ8L7U5. Positions 269-357, 550-832.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati60 – 10950Kelch 1Add
    BLAST
    Repeati269 – 32052Kelch 2Add
    BLAST
    Repeati338 – 38548Kelch 3Add
    BLAST

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. BSU subfamily.Curated
    Contains 3 Kelch repeats.Curated

    Keywords - Domaini

    Kelch repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000246464.
    InParanoidiQ8L7U5.
    KOiK01090.
    OMAiIVPKLIH.

    Family and domain databases

    Gene3Di2.120.10.80. 2 hits.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR015915. Kelch-typ_b-propeller.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    IPR012391. Ser/Thr_prot_Pase_BSU1.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036363. PPP_BSU1. 1 hit.
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8L7U5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSKPWLHPA PQYKTLETFW DDEDDAPGPR CAHTLTAVAA TKTHGPRLIL    50
    FGGATAIEGG SSSVPGIRLA GVTNTVHSYD ILTRKWTRLK PAGEPPSPRA 100
    AHAAAAVGTM VVFQGGIGPA GHSTDDLYVL DMTNDKFKWH RVVVQGDGPG 150
    PRYGHVMDLV SQRYLVTVTG NDGKRALSDA WALDTAQKPY VWQRLNPDGD 200
    RPSARMYASG SARSDGMFLL CGGRDTLGAP LGDAYGLLMH RNGQWEWTLA 250
    PGVAPSPRYQ HAAVFVGARL HVSGGVLRGG RVIDAEASVA VLDTAAGVWL 300
    DRNGQVTSAR GSKGQIDQDP SFELMRRCRH GAASVGIRIY VHGGLRGDVL 350
    LDDFLVAENS TFQSDISSPL LASDRTQQSS TPRFSYAARP PSGSEPSFSM 400
    SEGLSLDENS LEKLTEASAA EAEVASSVWR AAQLGAGTLD EEPSTSDASS 450
    PIVESTTDGT ANEGDVRLHP RAVVVAKETV GSLGGMVRQL SLDQFQNESR 500
    RMVPMNNSDV PQPTKKFTRQ KSPQGLHKKV IAALLRPRNW KPPGNRKFFL 550
    DSYEVGELCY AAEQIFMHEQ TVLQLKAPIK VFGDLHGQFG DLMRLFDEYG 600
    FPSTAGDITY IDYLFLGDYV DRGQHSLETI TLLLALKIEY PENVHLIRGN 650
    HEAADINALF GFRLECIERM GENDGIWAWT RFNQLFNYLP LAALIENKII 700
    CMHGGIGRSI STVEQIEKIE RPITMDAGSL VLMDLLWSDP TENDSIEGLR 750
    PNARGPGLVT FGPDRVTEFC KRNKLQLIIR AHECVMDGFE RFAQGQLITL 800
    FSATNYCGTA NNAGAILVVG RGLVIVPKLI HPLPPPILSP ENSPEHSGDD 850
    AWMQELNIQR PPTPTRGRPQ PDFDRSSLAY I 881
    Length:881
    Mass (Da):96,146
    Last modified:May 3, 2011 - v2
    Checksum:i6EC45417BAEBD6E0
    GO

    Sequence cautioni

    The sequence AAC79097.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB77793.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti794 – 7941Q → P in AAM83219. (PubMed:10617198)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF069442 Genomic DNA. Translation: AAC79097.1. Sequence problems.
    AL161496 Genomic DNA. Translation: CAB77793.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE82268.1.
    AY126992 mRNA. Translation: AAM83219.1.
    PIRiT01385.
    RefSeqiNP_192217.2. NM_116542.4.
    UniGeneiAt.26278.
    At.48826.

    Genome annotation databases

    EnsemblPlantsiAT4G03080.1; AT4G03080.1; AT4G03080.
    GeneIDi828097.
    KEGGiath:AT4G03080.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF069442 Genomic DNA. Translation: AAC79097.1 . Sequence problems.
    AL161496 Genomic DNA. Translation: CAB77793.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE82268.1 .
    AY126992 mRNA. Translation: AAM83219.1 .
    PIRi T01385.
    RefSeqi NP_192217.2. NM_116542.4.
    UniGenei At.26278.
    At.48826.

    3D structure databases

    ProteinModelPortali Q8L7U5.
    SMRi Q8L7U5. Positions 269-357, 550-832.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 13388. 1 interaction.
    IntActi Q8L7U5. 1 interaction.
    STRINGi 3702.AT4G03080.1-P.

    Proteomic databases

    PaxDbi Q8L7U5.
    PRIDEi Q8L7U5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G03080.1 ; AT4G03080.1 ; AT4G03080 .
    GeneIDi 828097.
    KEGGi ath:AT4G03080.

    Organism-specific databases

    TAIRi AT4G03080.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000246464.
    InParanoidi Q8L7U5.
    KOi K01090.
    OMAi IVPKLIH.

    Enzyme and pathway databases

    BioCyci ARA:AT4G03080-MONOMER.

    Gene expression databases

    Genevestigatori Q8L7U5.

    Family and domain databases

    Gene3Di 2.120.10.80. 2 hits.
    3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR015915. Kelch-typ_b-propeller.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    IPR012391. Ser/Thr_prot_Pase_BSU1.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036363. PPP_BSU1. 1 hit.
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis."
      Mora-Garcia S., Vert G., Yin Y., Cano-Delgado A., Cheong H., Chory J.
      Genes Dev. 18:448-460(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    5. "Arabidopsis PPP family of serine/threonine phosphatases."
      Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
      Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-491, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
    7. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-491, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBSL1_ARATH
    AccessioniPrimary (citable) accession number: Q8L7U5
    Secondary accession number(s): Q9ZTA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2004
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3