Reviewed, UniProtKB/Swiss-Prot Q8L7U5 (BSL1_ARATH)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein phosphatase BSL1
    EC=3.1.3.16
Alternative name(s):
    BSU1-like protein 1

Gene names

Name:	BSL1
Ordered Locus Names:	At4g03080
ORF Names:	T4I9.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	881 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphatase involved in elongation process, probably by acting as a regulator of brassinolide signaling. Ref.3
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Expressed in mature cauline leaves and at the tip of influorescence, including flowers. Expressed at lower level in young tissues relative to older ones. Ref.3
Sequence similarities	Belongs to the PPP phosphatase family. BSU subfamily. Contains 3 Kelch repeats.
Sequence caution	The sequence AAC79097.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB77793.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Kelch repeat Repeat
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 881	881	Serine/threonine-protein phosphatase BSL1		PRO_0000058905
Regions
	Repeat	60 – 109	50	Kelch 1
	Repeat	269 – 320	52	Kelch 2
	Repeat	338 – 385	48	Kelch 3
Sites
	Active site	651	1	Proton donor By similarity
	Metal binding	584	1	Iron By similarity
	Metal binding	586	1	Iron By similarity
	Metal binding	618	1	Iron By similarity
	Metal binding	618	1	Manganese By similarity
	Metal binding	650	1	Manganese By similarity
	Metal binding	703	1	Manganese By similarity
	Metal binding	782	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8L7U5-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: CFC54406BBFAD6E1	FASTA	881	96,115
	10 20 30 40 50 60 MGSKPWLHPA PQYKTLETFW DDEDDAPGPR CAHTLTAVAA TKTHGPRLIL FGGATAIEGG 70 80 90 100 110 120 SSSVPGIRLA GVTNTVHSYD ILTRKWTRLK PAGEPPSPRA AHAAAAVGTM VVFQGGIGPA 130 140 150 160 170 180 GHSTDDLYVL DMTNDKFKWH RVVVQGDGPG PRYGHVMDLV SQRYLVTVTG NDGKRALSDA 190 200 210 220 230 240 WALDTAQKPY VWQRLNPDGD RPSARMYASG SARSDGMFLL CGGRDTLGAP LGDAYGLLMH 250 260 270 280 290 300 RNGQWEWTLA PGVAPSPRYQ HAAVFVGARL HVSGGVLRGG RVIDAEASVA VLDTAAGVWL 310 320 330 340 350 360 DRNGQVTSAR GSKGQIDQDP SFELMRRCRH GAASVGIRIY VHGGLRGDVL LDDFLVAENS 370 380 390 400 410 420 TFQSDISSPL LASDRTQQSS TPRFSYAARP PSGSEPSFSM SEGLSLDENS LEKLTEASAA 430 440 450 460 470 480 EAEVASSVWR AAQLGAGTLD EEPSTSDASS PIVESTTDGT ANEGDVRLHP RAVVVAKETV 490 500 510 520 530 540 GSLGGMVRQL SLDQFQNESR RMVPMNNSDV PQPTKKFTRQ KSPQGLHKKV IAALLRPRNW 550 560 570 580 590 600 KPPGNRKFFL DSYEVGELCY AAEQIFMHEQ TVLQLKAPIK VFGDLHGQFG DLMRLFDEYG 610 620 630 640 650 660 FPSTAGDITY IDYLFLGDYV DRGQHSLETI TLLLALKIEY PENVHLIRGN HEAADINALF 670 680 690 700 710 720 GFRLECIERM GENDGIWAWT RFNQLFNYLP LAALIENKII CMHGGIGRSI STVEQIEKIE 730 740 750 760 770 780 RPITMDAGSL VLMDLLWSDP TENDSIEGLR PNARGPGLVT FGPDRVTEFC KRNKLQLIIR 790 800 810 820 830 840 AHECVMDGFE RFAPGQLITL FSATNYCGTA NNAGAILVVG RGLVIVPKLI HPLPPPILSP 850 860 870 880 ENSPEHSGDD AWMQELNIQR PPTPTRGRPQ PDFDRSSLAY I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis." Mora-Garcia S., Vert G., Yin Y., Cano-Delgado A., Cheong H., Chory J. Genes Dev. 18:448-460(2004) [PubMed: 14977918] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[4]	"Arabidopsis PPP family of serine/threonine phosphatases." Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C. Trends Plant Sci. 12:169-176(2007) [PubMed: 17368080] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF069442 Genomic DNA. Translation: AAC79097.1. Sequence problems. AL161496 Genomic DNA. Translation: CAB77793.1. Sequence problems. AY126992 mRNA. Translation: AAM83219.1.
IPI	IPI00526850.
PIR	T01385.
3D structure databases
HSSP	HSSP built from PDB template 1IT6 based on UniProtKB P36873.
ModBase	Search...
Proteomic databases
PRIDE	Q8L7U5.
ProMEX	Q8L7U5.
Genome annotation databases
GenomeReviews	Gene locus AT4G03080 in contig CT486007_GR.
Organism-specific databases
TAIR	At4g03080.
Enzyme and pathway databases
BRENDA	3.1.3.16. 302.
Gene expression databases
ArrayExpress	Q8L7U5.
GermOnline	AT4G03080. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR015915. Kelch-typ_b-propeller. IPR006652. Kelch_1. IPR011498. Kelch_2. IPR004843. M-pesterase. IPR012391. PPP_BSU1. IPR006186. T_phtase_apaH. [Graphical view]
Gene3D	G3DSA:2.120.10.80. Kelch-typ_b-propeller. 2 hits.
PANTHER	PTHR11668. T_phtase_apaH. 1 hit.
Pfam	PF01344. Kelch_1. 1 hit. PF07646. Kelch_2. 1 hit. PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF036363. PPP_BSU1. 1 hit.
PRINTS	PR00114. STPHPHTASE.
ProDom	PD000252. T_phtase_apaH. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BSL1_ARATH

Accession

Primary (citable) accession number: Q8L7U5
Secondary accession number(s): Q9ZTA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)