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Protein

Ubiquitin-conjugating enzyme E2 20

Gene

UBC20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei119Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCyclin, Transferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 20 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 20
Ubiquitin carrier protein 20
Gene namesi
Name:UBC20
Ordered Locus Names:At1g50490
ORF Names:F11F12.16, F17J6.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G50490
TAIRilocus:2015819 AT1G50490

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003451861 – 180Ubiquitin-conjugating enzyme E2 20Add BLAST180

Proteomic databases

PaxDbiQ8L7T3
PRIDEiQ8L7T3

Expressioni

Tissue specificityi

Expressed in all tissues with cell division activities and in mature leaves.2 Publications

Developmental stagei

Expressed during the G2-M phases of the cell cycle.1 Publication

Inductioni

By the herbicide isoxaben and by biotic stresses.1 Publication

Gene expression databases

ExpressionAtlasiQ8L7T3 baseline and differential
GenevisibleiQ8L7T3 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT1G50490.1

Structurei

3D structure databases

ProteinModelPortaliQ8L7T3
SMRiQ8L7T3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0421 Eukaryota
ENOG4111IGV LUCA
HOGENOMiHOG000233454
InParanoidiQ8L7T3
KOiK06688
OMAiXVYEDLR
OrthoDBiEOG09360Q1A
PhylomeDBiQ8L7T3

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.101 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8L7T3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVNGYQGN TPADPPASNG SKQPAAPTKT VDSQSVLKRL QSELMGLMMG
60 70 80 90 100
GGPGISAFPE EDNIFCWKGT ITGSKDTVFE GTEYRLSLSF SNDYPFKPPK
110 120 130 140 150
VKFETCCFHP NVDVYGNICL DILQDKWSSA YDVRTILLSI QSLLGEPNIS
160 170 180
SPLNTQAAQL WSNQEEYRKM VEKLYKPPSA
Length:180
Mass (Da):19,792
Last modified:October 1, 2002 - v1
Checksum:i0FFFF2E7347D815B
GO

Sequence cautioni

The sequence AAF87880 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG51188 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72T → I in AAM67229 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY127574 mRNA Translation: AAM96887.1
DQ027034 mRNA Translation: AAY44860.1
AC012561 Genomic DNA Translation: AAF87880.1 Sequence problems.
AC079279 Genomic DNA Translation: AAG51188.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE32556.1
AK227382 mRNA Translation: BAE99389.1
AY088923 mRNA Translation: AAM67229.1
BT005855 mRNA Translation: AAO64790.1
PIRiD96541
RefSeqiNP_564572.1, NM_103932.4
UniGeneiAt.21610
At.71081

Genome annotation databases

EnsemblPlantsiAT1G50490.1; AT1G50490.1; AT1G50490
GeneIDi841471
GrameneiAT1G50490.1; AT1G50490.1; AT1G50490
KEGGiath:AT1G50490

Similar proteinsi

Entry informationi

Entry nameiUBC20_ARATH
AccessioniPrimary (citable) accession number: Q8L7T3
Secondary accession number(s): Q8L8L3, Q9C6Q4, Q9LPS2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2002
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome