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Q8L7T3

- UBC20_ARATH

UniProt

Q8L7T3 - UBC20_ARATH

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Protein

Ubiquitin-conjugating enzyme E2 20

Gene

UBC20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.2 Publications

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei119 – 1191Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. cell proliferation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Cyclin, Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G50490-MONOMER.
ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_185297. Separation of Sister Chromatids.
REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 20 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 20
Gene namesi
Name:UBC20
Ordered Locus Names:At1g50490
ORF Names:F11F12.16, F17J6.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G50490.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 180180Ubiquitin-conjugating enzyme E2 20PRO_0000345186Add
BLAST

Proteomic databases

PaxDbiQ8L7T3.
PRIDEiQ8L7T3.

Expressioni

Tissue specificityi

Expressed in all tissues with cell division activities and in mature leaves.2 Publications

Developmental stagei

Expressed during the G2-M phases of the cell cycle.1 Publication

Inductioni

By the herbicide isoxaben and by biotic stresses.1 Publication

Gene expression databases

GenevestigatoriQ8L7T3.

Structurei

3D structure databases

ProteinModelPortaliQ8L7T3.
SMRiQ8L7T3. Positions 36-175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233454.
InParanoidiQ8L7T3.
KOiK06688.
OMAiMASANVQ.
PhylomeDBiQ8L7T3.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8L7T3 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAVNGYQGN TPADPPASNG SKQPAAPTKT VDSQSVLKRL QSELMGLMMG
60 70 80 90 100
GGPGISAFPE EDNIFCWKGT ITGSKDTVFE GTEYRLSLSF SNDYPFKPPK
110 120 130 140 150
VKFETCCFHP NVDVYGNICL DILQDKWSSA YDVRTILLSI QSLLGEPNIS
160 170 180
SPLNTQAAQL WSNQEEYRKM VEKLYKPPSA
Length:180
Mass (Da):19,792
Last modified:October 1, 2002 - v1
Checksum:i0FFFF2E7347D815B
GO

Sequence cautioni

The sequence AAF87880.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence AAG51188.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721T → I in AAM67229. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY127574 mRNA. Translation: AAM96887.1.
DQ027034 mRNA. Translation: AAY44860.1.
AC012561 Genomic DNA. Translation: AAF87880.1. Sequence problems.
AC079279 Genomic DNA. Translation: AAG51188.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32556.1.
AK227382 mRNA. Translation: BAE99389.1.
AY088923 mRNA. Translation: AAM67229.1.
BT005855 mRNA. Translation: AAO64790.1.
PIRiD96541.
RefSeqiNP_564572.1. NM_103932.3.
UniGeneiAt.21610.
At.71081.

Genome annotation databases

EnsemblPlantsiAT1G50490.1; AT1G50490.1; AT1G50490.
GeneIDi841471.
KEGGiath:AT1G50490.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY127574 mRNA. Translation: AAM96887.1 .
DQ027034 mRNA. Translation: AAY44860.1 .
AC012561 Genomic DNA. Translation: AAF87880.1 . Sequence problems.
AC079279 Genomic DNA. Translation: AAG51188.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE32556.1 .
AK227382 mRNA. Translation: BAE99389.1 .
AY088923 mRNA. Translation: AAM67229.1 .
BT005855 mRNA. Translation: AAO64790.1 .
PIRi D96541.
RefSeqi NP_564572.1. NM_103932.3.
UniGenei At.21610.
At.71081.

3D structure databases

ProteinModelPortali Q8L7T3.
SMRi Q8L7T3. Positions 36-175.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q8L7T3.
PRIDEi Q8L7T3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G50490.1 ; AT1G50490.1 ; AT1G50490 .
GeneIDi 841471.
KEGGi ath:AT1G50490.

Organism-specific databases

GeneFarmi 2577. 224.
TAIRi AT1G50490.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233454.
InParanoidi Q8L7T3.
KOi K06688.
OMAi MASANVQ.
PhylomeDBi Q8L7T3.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT1G50490-MONOMER.
Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_185297. Separation of Sister Chromatids.
REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Miscellaneous databases

PROi Q8L7T3.

Gene expression databases

Genevestigatori Q8L7T3.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family."
    Criqui M.C., de Almeida Engler J., Camasses A., Capron A., Parmentier Y., Inze D., Genschik P.
    Plant Physiol. 130:1230-1240(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, NOMENCLATURE.
  3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  8. "Arabidopsis anaphase-promoting complexes: multiple activators and wide range of substrates might keep APC perpetually busy."
    Fueloep K., Tarayre S., Kelemen Z., Horvath G., Kevei Z., Nikovics K., Bako L., Brown S., Kondorosi A., Kondorosi E.
    Cell Cycle 4:1084-1092(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiUBC20_ARATH
AccessioniPrimary (citable) accession number: Q8L7T3
Secondary accession number(s): Q8L8L3, Q9C6Q4, Q9LPS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2002
Last modified: October 1, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3