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Protein

Beta-hexosaminidase 3

Gene

HEXO3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a broad substrate specificity. Can use synthetic substrates such as pyridylaminated chitotriose, p-nitrophenyl-beta-N-acetylglucosaminide, p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (pNP-GlcNAc), p-nitrophenyl-2-acetamido-2-deoxy-beta-D-galactopyranoside (pNP-GalNAc), 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-GlcNAc), and 4-methylumbelliferyl-6-sulfo-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-GlcNAc-6SO4) as substrates. Removes terminal GlcNAc residues from alpha1,3- and alpha1,6-mannosyl branches of biantennary N-glycans without any strict branch preference. Required for the presence of paucimannosidic N-glycans in glycoproteins of roots and leaves.3 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

Enzyme regulationi

Slightly inhibited by N-acetylcastanospermine.1 Publication

Kineticsi

  1. KM=2.2 mM for pNP-GlcNAc (at pH 4.6 and 37 degrees Celsius)2 Publications
  1. Vmax=50.3 µmol/min/mg enzyme with pNP-GlcNAc as substrate (at pH 4.6 and 37 degrees Celsius)2 Publications

pH dependencei

Optimum pH is 4-5.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei329Proton donorBy similarity1

GO - Molecular functioni

  • beta-N-acetylhexosaminidase activity Source: TAIR
  • hexosaminidase activity Source: TAIR
  • N-acetyl-beta-D-galactosaminidase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G65590-MONOMER
BRENDAi3.2.1.52 399
ReactomeiR-ATH-1660662 Glycosphingolipid metabolism
R-ATH-2022857 Keratan sulfate degradation
R-ATH-2024101 CS/DS degradation
R-ATH-2160916 Hyaluronan uptake and degradation
R-ATH-6798695 Neutrophil degranulation

Protein family/group databases

CAZyiGH20 Glycoside Hydrolase Family 20

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidase 3 (EC:3.2.1.52)
Alternative name(s):
Beta-GlcNAcase 3
Beta-N-acetylhexosaminidase 3
Beta-hexosaminidase 1
Short name:
AtHEX1
N-acetyl-beta-glucosaminidase 3
Gene namesi
Name:HEXO3
Synonyms:HEX1
Ordered Locus Names:At1g65590
ORF Names:F5I14.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G65590
TAIRilocus:2034147 AT1G65590

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced amounts of paucimannosidic N-glycans-containing glycoproteins in roots and leaves.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000042028825 – 535Beta-hexosaminidase 3Add BLAST511

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi92N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi292 ↔ 334By similarity
Glycosylationi331N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi405N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi441N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi496N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi506 ↔ 532By similarity

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8L7S6
PRIDEiQ8L7S6

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, flowers and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ8L7S6 baseline and differential
GenevisibleiQ8L7S6 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G65590.1

Structurei

3D structure databases

ProteinModelPortaliQ8L7S6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 20 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2499 Eukaryota
COG3525 LUCA
HOGENOMiHOG000157972
InParanoidiQ8L7S6
KOiK12373
OMAiDSSFPYE
OrthoDBiEOG093609RX
PhylomeDBiQ8L7S6

Family and domain databases

Gene3Di3.30.379.10, 1 hit
InterProiView protein in InterPro
IPR025705 Beta_hexosaminidase_sua/sub
IPR015883 Glyco_hydro_20_cat
IPR017853 Glycoside_hydrolase_SF
IPR029018 Hex-like_dom2
IPR029019 HEX_eukaryotic_N
PfamiView protein in Pfam
PF00728 Glyco_hydro_20, 1 hit
PF14845 Glycohydro_20b2, 1 hit
PIRSFiPIRSF001093 B-hxosamndse_ab_euk_, 1 hit
PRINTSiPR00738 GLHYDRLASE20
SUPFAMiSSF51445 SSF51445, 1 hit
SSF55545 SSF55545, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L7S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGSGAKIAG VLPLFMLFIA GTISAFEDIE RLRIWPLPAQ VSHGGRRMYL
60 70 80 90 100
SGDFKLVTEG SKYGDASGIL KEGFDRMLGV VRLSHVISGD RNSSGTGGSA
110 120 130 140 150
LLQGLHVIIS SSTDELEYGA DESYKLVVPS PEKPSYAQLE AKSVYGALHG
160 170 180 190 200
LQTFSQLCHF NLKKKVIEIL MTPWNIIDQP RFSYRGLLID TSRHYLPLPV
210 220 230 240 250
IKNVIDSMTY AKLNVLHWHI VDTQSFPLEI PSYPKLWNGA YSSSQRYTFE
260 270 280 290 300
DAAEIVNYAR RRGIHVLAEI DVPGHALSWG KGYPALWPSK NCQEPLDVSS
310 320 330 340 350
DFTFKVIDGI LSDFSKIFKF KFVHLGGDEV NTTCWSATPR IAQWLKKHRM
360 370 380 390 400
SEKEAYQYFV LRAQKIALSH GYEIINWEET FINFGSKLNR KTVVHNWLNT
410 420 430 440 450
GLVENVTASG LRCIVSNQEF WYLDHIDAPW QGFYANEPFQ NITDKKQQSL
460 470 480 490 500
VLGGEVCMWG EHIDASDIEQ TIWPRAAAAA ERLWTPYAKL AKNPNNVTTR
510 520 530
LAHFRCLLNQ RGVAAAPLVG GGRVVPFEPG SCLAQ
Length:535
Mass (Da):60,014
Last modified:October 1, 2002 - v1
Checksum:i9295E14DB073F966
GO

Sequence cautioni

The sequence AAB60911 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC001229 Genomic DNA Translation: AAB60911.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE34399.1
AY128283 mRNA Translation: AAM91092.1
BT000831 mRNA Translation: AAN33206.1
PIRiA96681
RefSeqiNP_176737.2, NM_105233.5
UniGeneiAt.24164

Genome annotation databases

EnsemblPlantsiAT1G65590.1; AT1G65590.1; AT1G65590
GeneIDi842871
GrameneiAT1G65590.1; AT1G65590.1; AT1G65590
KEGGiath:AT1G65590

Similar proteinsi

Entry informationi

Entry nameiHEXO3_ARATH
AccessioniPrimary (citable) accession number: Q8L7S6
Secondary accession number(s): O04477
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: October 1, 2002
Last modified: April 25, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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