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Protein

Beta-hexosaminidase 3

Gene

HEXO3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a broad substrate specificity. Can use synthetic substrates such as pyridylaminated chitotriose, p-nitrophenyl-beta-N-acetylglucosaminide, p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (pNP-GlcNAc), p-nitrophenyl-2-acetamido-2-deoxy-beta-D-galactopyranoside (pNP-GalNAc), 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-GlcNAc), and 4-methylumbelliferyl-6-sulfo-2-acetamido-2-deoxy-beta-D-glucopyranoside (MU-GlcNAc-6SO4) as substrates. Removes terminal GlcNAc residues from alpha1,3- and alpha1,6-mannosyl branches of biantennary N-glycans without any strict branch preference. Required for the presence of paucimannosidic N-glycans in glycoproteins of roots and leaves.3 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

Enzyme regulationi

Slightly inhibited by N-acetylcastanospermine.1 Publication

Kineticsi

  1. KM=2.2 mM for pNP-GlcNAc (at pH 4.6 and 37 degrees Celsius)2 Publications
  1. Vmax=50.3 µmol/min/mg enzyme with pNP-GlcNAc as substrate (at pH 4.6 and 37 degrees Celsius)2 Publications

pH dependencei

Optimum pH is 4-5.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei329Proton donorBy similarity1

GO - Molecular functioni

  • beta-N-acetylhexosaminidase activity Source: TAIR
  • hexosaminidase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G65590-MONOMER.
BRENDAi3.2.1.52. 399.
ReactomeiR-ATH-1660662. Glycosphingolipid metabolism.
R-ATH-2022857. Keratan sulfate degradation.
R-ATH-2024101. CS/DS degradation.
R-ATH-2160916. Hyaluronan uptake and degradation.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidase 3 (EC:3.2.1.52)
Alternative name(s):
Beta-GlcNAcase 3
Beta-N-acetylhexosaminidase 3
Beta-hexosaminidase 1
Short name:
AtHEX1
N-acetyl-beta-glucosaminidase 3
Gene namesi
Name:HEXO3
Synonyms:HEX1
Ordered Locus Names:At1g65590
ORF Names:F5I14.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G65590.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • plant-type cell wall Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced amounts of paucimannosidic N-glycans-containing glycoproteins in roots and leaves.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000042028825 – 535Beta-hexosaminidase 3Add BLAST511

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi92N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi292 ↔ 334By similarity
Glycosylationi331N-linked (GlcNAc...)Sequence analysis1
Glycosylationi405N-linked (GlcNAc...)Sequence analysis1
Glycosylationi441N-linked (GlcNAc...)Sequence analysis1
Glycosylationi496N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi506 ↔ 532By similarity

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8L7S6.
PRIDEiQ8L7S6.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, flowers and siliques.1 Publication

Gene expression databases

GenevisibleiQ8L7S6. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G65590.1.

Structurei

3D structure databases

ProteinModelPortaliQ8L7S6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 20 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2499. Eukaryota.
COG3525. LUCA.
HOGENOMiHOG000157972.
InParanoidiQ8L7S6.
KOiK12373.
OMAiNTSCWTS.
OrthoDBiEOG093609RX.
PhylomeDBiQ8L7S6.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamiPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSiPR00738. GLHYDRLASE20.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L7S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGSGAKIAG VLPLFMLFIA GTISAFEDIE RLRIWPLPAQ VSHGGRRMYL
60 70 80 90 100
SGDFKLVTEG SKYGDASGIL KEGFDRMLGV VRLSHVISGD RNSSGTGGSA
110 120 130 140 150
LLQGLHVIIS SSTDELEYGA DESYKLVVPS PEKPSYAQLE AKSVYGALHG
160 170 180 190 200
LQTFSQLCHF NLKKKVIEIL MTPWNIIDQP RFSYRGLLID TSRHYLPLPV
210 220 230 240 250
IKNVIDSMTY AKLNVLHWHI VDTQSFPLEI PSYPKLWNGA YSSSQRYTFE
260 270 280 290 300
DAAEIVNYAR RRGIHVLAEI DVPGHALSWG KGYPALWPSK NCQEPLDVSS
310 320 330 340 350
DFTFKVIDGI LSDFSKIFKF KFVHLGGDEV NTTCWSATPR IAQWLKKHRM
360 370 380 390 400
SEKEAYQYFV LRAQKIALSH GYEIINWEET FINFGSKLNR KTVVHNWLNT
410 420 430 440 450
GLVENVTASG LRCIVSNQEF WYLDHIDAPW QGFYANEPFQ NITDKKQQSL
460 470 480 490 500
VLGGEVCMWG EHIDASDIEQ TIWPRAAAAA ERLWTPYAKL AKNPNNVTTR
510 520 530
LAHFRCLLNQ RGVAAAPLVG GGRVVPFEPG SCLAQ
Length:535
Mass (Da):60,014
Last modified:October 1, 2002 - v1
Checksum:i9295E14DB073F966
GO

Sequence cautioni

The sequence AAB60911 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC001229 Genomic DNA. Translation: AAB60911.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34399.1.
AY128283 mRNA. Translation: AAM91092.1.
BT000831 mRNA. Translation: AAN33206.1.
PIRiA96681.
RefSeqiNP_176737.2. NM_105233.5.
UniGeneiAt.24164.

Genome annotation databases

EnsemblPlantsiAT1G65590.1; AT1G65590.1; AT1G65590.
GeneIDi842871.
GrameneiAT1G65590.1; AT1G65590.1; AT1G65590.
KEGGiath:AT1G65590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC001229 Genomic DNA. Translation: AAB60911.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34399.1.
AY128283 mRNA. Translation: AAM91092.1.
BT000831 mRNA. Translation: AAN33206.1.
PIRiA96681.
RefSeqiNP_176737.2. NM_105233.5.
UniGeneiAt.24164.

3D structure databases

ProteinModelPortaliQ8L7S6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G65590.1.

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Proteomic databases

PaxDbiQ8L7S6.
PRIDEiQ8L7S6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G65590.1; AT1G65590.1; AT1G65590.
GeneIDi842871.
GrameneiAT1G65590.1; AT1G65590.1; AT1G65590.
KEGGiath:AT1G65590.

Organism-specific databases

TAIRiAT1G65590.

Phylogenomic databases

eggNOGiKOG2499. Eukaryota.
COG3525. LUCA.
HOGENOMiHOG000157972.
InParanoidiQ8L7S6.
KOiK12373.
OMAiNTSCWTS.
OrthoDBiEOG093609RX.
PhylomeDBiQ8L7S6.

Enzyme and pathway databases

BioCyciARA:AT1G65590-MONOMER.
BRENDAi3.2.1.52. 399.
ReactomeiR-ATH-1660662. Glycosphingolipid metabolism.
R-ATH-2022857. Keratan sulfate degradation.
R-ATH-2024101. CS/DS degradation.
R-ATH-2160916. Hyaluronan uptake and degradation.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ8L7S6.

Gene expression databases

GenevisibleiQ8L7S6. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamiPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSiPR00738. GLHYDRLASE20.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHEXO3_ARATH
AccessioniPrimary (citable) accession number: Q8L7S6
Secondary accession number(s): O04477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.