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Q8L7R2

- KHSE_ARATH

UniProt

Q8L7R2 - KHSE_ARATH

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Protein
Homoserine kinase
Gene
HSK, DMR1, At2g17265, F5J6.24
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate. Is specific for L-homoserine and cannot use other substrates such D-serine, L-serine, D-threonine and L-threonine, galactose or D-homoserine in vitro. Required for susceptibility to the downy mildew pathogen Hyaloperonospora parasitica.4 Publications

Catalytic activityi

ATP + L-homoserine = ADP + O-phospho-L-homoserine.UniRule annotation

Kineticsi

  1. KM=0.40 mM for L-homoserine1 Publication
  2. KM=0.32 mM for ATP

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi143 – 15412ATP Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. homoserine kinase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. defense response Source: UniProtKB-KW
  2. methionine biosynthetic process Source: TAIR
  3. response to bacterium Source: TAIR
  4. response to fungus Source: TAIR
  5. threonine biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Plant defense, Threonine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-1961.
UniPathwayiUPA00050; UER00064.

Names & Taxonomyi

Protein namesi
Recommended name:
Homoserine kinase (EC:2.7.1.39)
Alternative name(s):
Protein DOWNY MILDEW RESISTANT 1
Gene namesi
Name:HSK
Synonyms:DMR1
Ordered Locus Names:At2g17265
ORF Names:F5J6.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G17265.

Subcellular locationi

Plastidchloroplast stroma Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plant accumulate homoserine and show reduced susceptibility to the downy mildew pathogen Hyaloperonospora parasitica.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi46 – 461E → K in dmr1-2; loss of function. 1 Publication
Mutagenesisi118 – 1181G → R in dmr1-6; loss of function. 1 Publication
Mutagenesisi180 – 1801G → D in dmr1-5; loss of function. 1 Publication
Mutagenesisi202 – 2021G → R in dmr1-4; loss of function. 1 Publication
Mutagenesisi241 – 2411M → K in dmr1-3; loss of function. 1 Publication
Mutagenesisi267 – 2671A → V in dmr1-1; loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434Chloroplast Reviewed prediction
Add
BLAST
Chaini35 – 370336Homoserine kinaseUniRule annotation
PRO_0000428662Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriQ8L7R2.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G17265.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8L7R2.
SMRiQ8L7R2. Positions 49-365.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi153 – 16210Poly-AlaUniRule annotation

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiQ8L7R2.
KOiK00872.
OMAiKENIVYQ.
PhylomeDBiQ8L7R2.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00384. Homoser_kinase.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR000870. Homoserine_kinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000676. Homoser_kin. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00191. thrB. 1 hit.
PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L7R2-1 [UniParc]FASTAAdd to Basket

« Hide

MASLCFQSPS KPISYFQPKS NPSPPLFAKV SVFRCRASVQ TLVAVEPEPV    50
FVSVKTFAPA TVANLGPGFD FLGCAVDGLG DHVTLRVDPS VRAGEVSISE 100
ITGTTTKLST NPLRNCAGIA AIATMKMLGI RSVGLSLDLH KGLPLGSGLG 150
SSAASAAAAA VAVNEIFGRK LGSDQLVLAG LESEAKVSGY HADNIAPAIM 200
GGFVLIRNYE PLDLKPLRFP SDKDLFFVLV SPDFEAPTKK MRAALPTEIP 250
MVHHVWNSSQ AAALVAAVLE GDAVMLGKAL SSDKIVEPTR APLIPGMEAV 300
KKAALEAGAF GCTISGAGPT AVAVIDSEEK GQVIGEKMVE AFWKVGHLKS 350
VASVKKLDNV GARLVNSVSR 370
Length:370
Mass (Da):38,529
Last modified:October 1, 2002 - v1
Checksum:i50FB7315AF061889
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321V → F in BAC42512. 1 Publication
Sequence conflicti218 – 2181R → K in AAD33097. 1 Publication
Sequence conflicti233 – 2331D → E in AAD33097. 1 Publication
Sequence conflicti359 – 3591N → K in AAD33097. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF082525 mRNA. Translation: AAD33097.1.
CP002685 Genomic DNA. Translation: AEC06605.1.
AK117871 mRNA. Translation: BAC42512.2.
AY128313 mRNA. Translation: AAM91516.1.
BT001174 mRNA. Translation: AAN65061.1.
RefSeqiNP_179318.1. NM_127281.1.
UniGeneiAt.10492.
At.26823.

Genome annotation databases

EnsemblPlantsiAT2G17265.1; AT2G17265.1; AT2G17265.
GeneIDi816232.
KEGGiath:AT2G17265.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF082525 mRNA. Translation: AAD33097.1 .
CP002685 Genomic DNA. Translation: AEC06605.1 .
AK117871 mRNA. Translation: BAC42512.2 .
AY128313 mRNA. Translation: AAM91516.1 .
BT001174 mRNA. Translation: AAN65061.1 .
RefSeqi NP_179318.1. NM_127281.1.
UniGenei At.10492.
At.26823.

3D structure databases

ProteinModelPortali Q8L7R2.
SMRi Q8L7R2. Positions 49-365.
ModBasei Search...

Protein-protein interaction databases

STRINGi 3702.AT2G17265.1-P.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G17265.1 ; AT2G17265.1 ; AT2G17265 .
GeneIDi 816232.
KEGGi ath:AT2G17265.

Organism-specific databases

TAIRi AT2G17265.

Phylogenomic databases

InParanoidi Q8L7R2.
KOi K00872.
OMAi KENIVYQ.
PhylomeDBi Q8L7R2.

Enzyme and pathway databases

UniPathwayi UPA00050 ; UER00064 .
BioCyci MetaCyc:MONOMER-1961.

Miscellaneous databases

PROi Q8L7R2.

Gene expression databases

Genevestigatori Q8L7R2.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPi MF_00384. Homoser_kinase.
InterProi IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR000870. Homoserine_kinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view ]
Pfami PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000676. Homoser_kin. 1 hit.
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsi TIGR00191. thrB. 1 hit.
PROSITEi PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the gene encoding homoserine kinase from Arabidopsis thaliana and characterization of the recombinant enzyme derived from the gene."
    Lee M., Leustek T.
    Arch. Biochem. Biophys. 372:135-142(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Identification of arabidopsis loci required for susceptibility to the downy mildew pathogen Hyaloperonospora parasitica."
    Van Damme M., Andel A., Huibers R.P., Panstruga R., Weisbeek P.J., Van den Ackerveken G.
    Mol. Plant Microbe Interact. 18:583-592(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "Methionine and threonine synthesis are limited by homoserine availability and not the activity of homoserine kinase in Arabidopsis thaliana."
    Lee M., Martin M.N., Hudson A.O., Lee J., Muhitch M.J., Leustek T.
    Plant J. 41:685-696(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-46; GLY-118; GLY-180; GLY-202; MET-241 AND ALA-267.

Entry informationi

Entry nameiKHSE_ARATH
AccessioniPrimary (citable) accession number: Q8L7R2
Secondary accession number(s): Q7FLV1, Q9XEE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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