Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8L7R2

- KHSE_ARATH

UniProt

Q8L7R2 - KHSE_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Homoserine kinase

Gene

HSK

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate. Is specific for L-homoserine and cannot use other substrates such D-serine, L-serine, D-threonine and L-threonine, galactose or D-homoserine in vitro. Required for susceptibility to the downy mildew pathogen Hyaloperonospora parasitica.4 Publications

Catalytic activityi

ATP + L-homoserine = ADP + O-phospho-L-homoserine.

Kineticsi

  1. KM=0.40 mM for L-homoserine1 Publication
  2. KM=0.32 mM for ATP1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi143 – 15412ATPSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. homoserine kinase activity Source: TAIR

GO - Biological processi

  1. defense response Source: UniProtKB-KW
  2. methionine biosynthetic process Source: TAIR
  3. response to bacterium Source: TAIR
  4. response to fungus Source: TAIR
  5. threonine biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Plant defense, Threonine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-1961.
UniPathwayiUPA00050; UER00064.

Names & Taxonomyi

Protein namesi
Recommended name:
Homoserine kinase (EC:2.7.1.39)
Alternative name(s):
Protein DOWNY MILDEW RESISTANT 1
Gene namesi
Name:HSK
Synonyms:DMR1
Ordered Locus Names:At2g17265
ORF Names:F5J6.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G17265.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plant accumulate homoserine and show reduced susceptibility to the downy mildew pathogen Hyaloperonospora parasitica.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi46 – 461E → K in dmr1-2; loss of function. 1 Publication
Mutagenesisi118 – 1181G → R in dmr1-6; loss of function. 1 Publication
Mutagenesisi180 – 1801G → D in dmr1-5; loss of function. 1 Publication
Mutagenesisi202 – 2021G → R in dmr1-4; loss of function. 1 Publication
Mutagenesisi241 – 2411M → K in dmr1-3; loss of function. 1 Publication
Mutagenesisi267 – 2671A → V in dmr1-1; loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434ChloroplastSequence AnalysisAdd
BLAST
Chaini35 – 370336Homoserine kinasePRO_0000428662Add
BLAST

Expressioni

Gene expression databases

ExpressionAtlasiQ8L7R2. baseline and differential.
GenevestigatoriQ8L7R2.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G17265.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8L7R2.
SMRiQ8L7R2. Positions 53-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi153 – 16210Poly-Ala

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiQ8L7R2.
KOiK00872.
OMAiKENIVYQ.
PhylomeDBiQ8L7R2.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00384. Homoser_kinase.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR000870. Homoserine_kinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000676. Homoser_kin. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00191. thrB. 1 hit.
PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L7R2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASLCFQSPS KPISYFQPKS NPSPPLFAKV SVFRCRASVQ TLVAVEPEPV
60 70 80 90 100
FVSVKTFAPA TVANLGPGFD FLGCAVDGLG DHVTLRVDPS VRAGEVSISE
110 120 130 140 150
ITGTTTKLST NPLRNCAGIA AIATMKMLGI RSVGLSLDLH KGLPLGSGLG
160 170 180 190 200
SSAASAAAAA VAVNEIFGRK LGSDQLVLAG LESEAKVSGY HADNIAPAIM
210 220 230 240 250
GGFVLIRNYE PLDLKPLRFP SDKDLFFVLV SPDFEAPTKK MRAALPTEIP
260 270 280 290 300
MVHHVWNSSQ AAALVAAVLE GDAVMLGKAL SSDKIVEPTR APLIPGMEAV
310 320 330 340 350
KKAALEAGAF GCTISGAGPT AVAVIDSEEK GQVIGEKMVE AFWKVGHLKS
360 370
VASVKKLDNV GARLVNSVSR
Length:370
Mass (Da):38,529
Last modified:October 1, 2002 - v1
Checksum:i50FB7315AF061889
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321V → F in BAC42512. (PubMed:11910074)Curated
Sequence conflicti218 – 2181R → K in AAD33097. (PubMed:10562426)Curated
Sequence conflicti233 – 2331D → E in AAD33097. (PubMed:10562426)Curated
Sequence conflicti359 – 3591N → K in AAD33097. (PubMed:10562426)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF082525 mRNA. Translation: AAD33097.1.
CP002685 Genomic DNA. Translation: AEC06605.1.
AK117871 mRNA. Translation: BAC42512.2.
AY128313 mRNA. Translation: AAM91516.1.
BT001174 mRNA. Translation: AAN65061.1.
RefSeqiNP_179318.1. NM_127281.1.
UniGeneiAt.10492.
At.26823.

Genome annotation databases

EnsemblPlantsiAT2G17265.1; AT2G17265.1; AT2G17265.
GeneIDi816232.
KEGGiath:AT2G17265.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF082525 mRNA. Translation: AAD33097.1 .
CP002685 Genomic DNA. Translation: AEC06605.1 .
AK117871 mRNA. Translation: BAC42512.2 .
AY128313 mRNA. Translation: AAM91516.1 .
BT001174 mRNA. Translation: AAN65061.1 .
RefSeqi NP_179318.1. NM_127281.1.
UniGenei At.10492.
At.26823.

3D structure databases

ProteinModelPortali Q8L7R2.
SMRi Q8L7R2. Positions 53-358.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G17265.1-P.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G17265.1 ; AT2G17265.1 ; AT2G17265 .
GeneIDi 816232.
KEGGi ath:AT2G17265.

Organism-specific databases

TAIRi AT2G17265.

Phylogenomic databases

InParanoidi Q8L7R2.
KOi K00872.
OMAi KENIVYQ.
PhylomeDBi Q8L7R2.

Enzyme and pathway databases

UniPathwayi UPA00050 ; UER00064 .
BioCyci MetaCyc:MONOMER-1961.

Miscellaneous databases

PROi Q8L7R2.

Gene expression databases

ExpressionAtlasi Q8L7R2. baseline and differential.
Genevestigatori Q8L7R2.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPi MF_00384. Homoser_kinase.
InterProi IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR000870. Homoserine_kinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view ]
Pfami PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000676. Homoser_kin. 1 hit.
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsi TIGR00191. thrB. 1 hit.
PROSITEi PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the gene encoding homoserine kinase from Arabidopsis thaliana and characterization of the recombinant enzyme derived from the gene."
    Lee M., Leustek T.
    Arch. Biochem. Biophys. 372:135-142(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Identification of arabidopsis loci required for susceptibility to the downy mildew pathogen Hyaloperonospora parasitica."
    Van Damme M., Andel A., Huibers R.P., Panstruga R., Weisbeek P.J., Van den Ackerveken G.
    Mol. Plant Microbe Interact. 18:583-592(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "Methionine and threonine synthesis are limited by homoserine availability and not the activity of homoserine kinase in Arabidopsis thaliana."
    Lee M., Martin M.N., Hudson A.O., Lee J., Muhitch M.J., Leustek T.
    Plant J. 41:685-696(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-46; GLY-118; GLY-180; GLY-202; MET-241 AND ALA-267.

Entry informationi

Entry nameiKHSE_ARATH
AccessioniPrimary (citable) accession number: Q8L7R2
Secondary accession number(s): Q7FLV1, Q9XEE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3