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Q8L7L8 (TRP1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Telomere repeat-binding protein 1

Short name=AtTRP1
Gene names
Name:TRP1
Ordered Locus Names:At5g59430
ORF Names:F2O15.20
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length578 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds specifically to the plant telomeric double-stranded DNA sequences 5'-GGTTTAG-3'. At least 4 repeats of telomeric sequences are required for binding. Induces DNA bending.

Subunit structure

Homodimer and heterodimer with TRP2 and TRP3. Interacts with KU70. Ref.7 Ref.8

Subcellular location

Nucleus By similarity.

Tissue specificity

Expressed ubiquitously. Highest expression in flowers and leaves. Ref.8 Ref.9

Domain

A N-terminal domain (80-269) is responsible for the interaction with KU70.

The C-terminal domain (464-578) is sufficient for telomere binding.

Disruption phenotype

No visible phenotype, probably due to redundancy. Ref.8

Sequence similarities

Contains 1 HTH myb-type DNA-binding domain.

Contains 1 ubiquitin-like domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processethylene-activated signaling pathway

Inferred from mutant phenotype PubMed 19567478. Source: TAIR

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

response to auxin

Inferred from expression pattern Ref.10. Source: TAIR

response to cadmium ion

Inferred from expression pattern Ref.10. Source: TAIR

response to ethylene

Inferred from expression pattern Ref.10. Source: TAIR

response to gibberellin

Inferred from expression pattern Ref.10. Source: TAIR

response to jasmonic acid

Inferred from expression pattern Ref.10. Source: TAIR

response to salicylic acid

Inferred from expression pattern Ref.10. Source: TAIR

response to salt stress

Inferred from expression pattern Ref.10. Source: TAIR

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 19567478. Source: TAIR

membrane

Inferred from direct assay PubMed 19567478. Source: TAIR

nuclear membrane

Inferred from direct assay PubMed 19567478. Source: TAIR

plasma membrane

Inferred from direct assay PubMed 19567478. Source: TAIR

   Molecular_functionchromatin binding

Inferred from electronic annotation. Source: InterPro

double-stranded telomeric DNA binding

Inferred from direct assay Ref.1. Source: TAIR

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8L7L8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8L7L8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     555-578: QLQQNVNKLEQETQSQTTEGLLLL → PWRSSLLCVLLLLLMIM
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8L7L8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     555-578: QLQQNVNKLEQETQSQTTEGLLLL → HLGGRLSFVYYYYY
Note: Derived from EST data. No experimental confirmation available.
Isoform 4 (identifier: Q8L7L8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     29-29: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 578578Telomere repeat-binding protein 1
PRO_0000394124

Regions

Domain293 – 37280Ubiquitin-like
Domain463 – 52260HTH myb-type
DNA binding491 – 51828H-T-H motif By similarity
Region465 – 4695Interaction with DNA
Region511 – 5155Interaction with DNA
Region522 – 5298Interaction with DNA
Compositional bias457 – 4615Poly-Gln
Compositional bias575 – 5784Poly-Leu

Natural variations

Alternative sequence291Missing in isoform 4.
VSP_039133
Alternative sequence555 – 57824QLQQN…GLLLL → PWRSSLLCVLLLLLMIM in isoform 2.
VSP_039134
Alternative sequence555 – 57824QLQQN…GLLLL → HLGGRLSFVYYYYY in isoform 3.
VSP_039135

Experimental info

Sequence conflict41H → R in CAB50690. Ref.1
Sequence conflict581S → P in CAB50690. Ref.1
Sequence conflict1371D → E in BAH19498. Ref.6
Sequence conflict4731V → A in AAM91585. Ref.5
Sequence conflict4731V → A in AAN15436. Ref.5

Secondary structure

............... 578
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: 853B54C5FC2E5E01

FASTA57865,036
        10         20         30         40         50         60 
MVSHKCVEEF GYASYLVPSN ARAPRSARKR RSIEKRISKE DDNMCAIDLL ATVAGHLSFE 

        70         80         90        100        110        120 
SGSSLMSIDK LIEDHRVKEE FPEEEKPLMP VALSPYRGSL SPCGFSSVIN GKVENEVDGF 

       130        140        150        160        170        180 
SYSGGSDACQ VGNFSQDVKP DIDGDAVVLD ARPNVVVSLG SSSRTEVPSI GNCVSHGVRD 

       190        200        210        220        230        240 
DVNLFSRDDD ENFSKYIHPR VTKHSPRTVP RIGDRRIRKI LASRHWKGGS RHSDTKPWRN 

       250        260        270        280        290        300 
YYLHQQRSYP IKKRKNFDHI SDSVTDDYRM RTKMHRGSRK GQGASFVASD SHVKLRIKSF 

       310        320        330        340        350        360 
RVPELFIEIP ETATVGSLKR MVMEAVSTLL SDGHRVGLMV QGKKVRDDNK TLHQTGISQD 

       370        380        390        400        410        420 
NSHLDSLDFS LEPSSEMPQL LTSHPLGHAC EELLPVCQAT KIDNVLESDH HDSALFPSDS 

       430        440        450        460        470        480 
LGNNNVTEDS KAMISVALNE LSSQSQPPSR KSRRSEQQQQ QAAQRRIRRP FSVAEVEALV 

       490        500        510        520        530        540 
QAVEKLGTGR WRDVKLCAFE DADHRTYVDL KDKWKTLVHT AKISPQQRRG EPVPQELLNR 

       550        560        570 
VLNAHGYWTQ QQMQQLQQNV NKLEQETQSQ TTEGLLLL 

« Hide

Isoform 2 [UniParc].

Checksum: 17BCF837A70A75FE
Show »

FASTA57164,282
Isoform 3 [UniParc].

Checksum: 88CA324D8E8DDC3D
Show »

FASTA56864,081
Isoform 4 [UniParc].

Checksum: 6CF814FEE16B7055
Show »

FASTA57764,908

References

« Hide 'large scale' references
[1]"A plant gene encoding a Myb-like protein that binds telomeric GGTTTAG repeats in vitro."
Chen C.M., Wang C.T., Ho C.H.
J. Biol. Chem. 276:16511-16519(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DNA-BINDING.
[2]"The MYB transcription factor family in Arabidopsis: a genome-wide cloning and expression pattern analysis."
Qu L., Gu H.
Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. XI."
Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: cv. Columbia.
[7]"Interactions of putative telomere-binding proteins in Arabidopsis thaliana: identification of functional TRF2 homolog in plants."
Kuchar M., Fajkus J.
FEBS Lett. 578:311-315(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KU70.
[8]"A C-terminal Myb extension domain defines a novel family of double-strand telomeric DNA-binding proteins in Arabidopsis."
Karamysheva Z.N., Surovtseva Y.V., Vespa L., Shakirov E.V., Shippen D.E.
J. Biol. Chem. 279:47799-47807(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, TISSUE SPECIFICITY, SUBUNIT, DISRUPTION PHENOTYPE.
[9]"AtTBP2 and AtTRP2 in Arabidopsis encode proteins that bind plant telomeric DNA and induce DNA bending in vitro."
Hwang M.G., Kim K., Lee W.K., Cho M.H.
Mol. Genet. Genomics 273:66-75(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING, TISSUE SPECIFICITY.
[10]"The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family."
Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X., Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H., Qu L.-J.
Plant Mol. Biol. 60:107-124(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[11]"Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain: a new fold with an additional C-terminal helix."
Sue S.C., Hsiao H.H., Chung B.C., Cheng Y.H., Hsueh K.L., Chen C.M., Ho C.H., Huang T.H.
J. Mol. Biol. 356:72-85(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 464-560, DNA-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y17722 Genomic DNA. Translation: CAB50690.1.
AY519544 mRNA. Translation: AAS10014.1.
AB025604 Genomic DNA. Translation: BAA97479.1.
CP002688 Genomic DNA. Translation: AED97184.1.
CP002688 Genomic DNA. Translation: AED97185.1.
CP002688 Genomic DNA. Translation: AED97186.1.
CP002688 Genomic DNA. Translation: AED97187.1.
AY128382 mRNA. Translation: AAM91585.1.
BT000117 mRNA. Translation: AAN15436.1.
AK316779 mRNA. Translation: BAH19498.1.
AK318830 mRNA. Translation: BAH56945.1.
PIRT51230.
RefSeqNP_001078770.1. NM_001085301.1.
NP_001078771.1. NM_001085302.1.
NP_200751.1. NM_125334.3.
NP_851221.1. NM_180890.1.
UniGeneAt.42990.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2AJENMR-A464-560[»]
ProteinModelPortalQ8L7L8.
SMRQ8L7L8. Positions 439-560.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8L7L8. 4 interactions.
MINTMINT-1212501.
STRING3702.AT5G59430.2-P.

Proteomic databases

PRIDEQ8L7L8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G59430.1; AT5G59430.1; AT5G59430. [Q8L7L8-1]
AT5G59430.2; AT5G59430.2; AT5G59430. [Q8L7L8-1]
GeneID836062.
KEGGath:AT5G59430.

Organism-specific databases

TAIRAT5G59430.

Phylogenomic databases

eggNOGNOG283818.
InParanoidQ9LTI6.
OMACAFEDAD.
PhylomeDBQ8L7L8.
ProtClustDBCLSN2686463.

Gene expression databases

GenevestigatorQ8L7L8.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR000626. Ubiquitin-like.
[Graphical view]
SMARTSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS51294. HTH_MYB. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8L7L8.

Entry information

Entry nameTRP1_ARATH
AccessionPrimary (citable) accession number: Q8L7L8
Secondary accession number(s): A8MQF8 expand/collapse secondary AC list , A8MQM5, B9DFI1, C0Z2L6, Q9LTI6, Q9XGN0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names