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Q8L7L8

- TRP1_ARATH

UniProt

Q8L7L8 - TRP1_ARATH

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Protein
Telomere repeat-binding protein 1
Gene
TRP1, At5g59430, F2O15.20
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binds specifically to the plant telomeric double-stranded DNA sequences 5'-GGTTTAG-3'. At least 4 repeats of telomeric sequences are required for binding. Induces DNA bending.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi491 – 51828H-T-H motif By similarity
Add
BLAST

GO - Molecular functioni

  1. chromatin binding Source: InterPro
  2. double-stranded telomeric DNA binding Source: TAIR
  3. protein binding Source: IntAct

GO - Biological processi

  1. ethylene-activated signaling pathway Source: TAIR
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. response to auxin Source: TAIR
  4. response to cadmium ion Source: TAIR
  5. response to ethylene Source: TAIR
  6. response to gibberellin Source: TAIR
  7. response to jasmonic acid Source: TAIR
  8. response to salicylic acid Source: TAIR
  9. response to salt stress Source: TAIR
  10. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Telomere repeat-binding protein 1
Short name:
AtTRP1
Gene namesi
Name:TRP1
Ordered Locus Names:At5g59430
ORF Names:F2O15.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G59430.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: TAIR
  2. membrane Source: TAIR
  3. nuclear membrane Source: TAIR
  4. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, probably due to redundancy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 578578Telomere repeat-binding protein 1
PRO_0000394124Add
BLAST

Proteomic databases

PRIDEiQ8L7L8.

Expressioni

Tissue specificityi

Expressed ubiquitously. Highest expression in flowers and leaves.2 Publications

Gene expression databases

GenevestigatoriQ8L7L8.

Interactioni

Subunit structurei

Homodimer and heterodimer with TRP2 and TRP3. Interacts with KU70.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ERS1Q388462EBI-476071,EBI-1606754
KU70Q9FQ082EBI-476071,EBI-476083

Protein-protein interaction databases

IntActiQ8L7L8. 4 interactions.
MINTiMINT-1212501.
STRINGi3702.AT5G59430.2-P.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi473 – 48614
Beta strandi488 – 4903
Helixi491 – 4955
Beta strandi496 – 5016
Helixi507 – 51913
Turni525 – 5295
Helixi536 – 55116
Turni552 – 5554
Beta strandi556 – 5583

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AJENMR-A464-560[»]
ProteinModelPortaliQ8L7L8.
SMRiQ8L7L8. Positions 439-560.

Miscellaneous databases

EvolutionaryTraceiQ8L7L8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini293 – 37280Ubiquitin-like
Add
BLAST
Domaini463 – 52260HTH myb-type
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni465 – 4695Interaction with DNA
Regioni511 – 5155Interaction with DNA
Regioni522 – 5298Interaction with DNA

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi457 – 4615Poly-Gln
Compositional biasi575 – 5784Poly-Leu

Domaini

A N-terminal domain (80-269) is responsible for the interaction with KU70.
The C-terminal domain (464-578) is sufficient for telomere binding.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG283818.
InParanoidiQ9LTI6.
OMAiCAFEDAD.
PhylomeDBiQ8L7L8.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51294. HTH_MYB. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8L7L8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVSHKCVEEF GYASYLVPSN ARAPRSARKR RSIEKRISKE DDNMCAIDLL    50
ATVAGHLSFE SGSSLMSIDK LIEDHRVKEE FPEEEKPLMP VALSPYRGSL 100
SPCGFSSVIN GKVENEVDGF SYSGGSDACQ VGNFSQDVKP DIDGDAVVLD 150
ARPNVVVSLG SSSRTEVPSI GNCVSHGVRD DVNLFSRDDD ENFSKYIHPR 200
VTKHSPRTVP RIGDRRIRKI LASRHWKGGS RHSDTKPWRN YYLHQQRSYP 250
IKKRKNFDHI SDSVTDDYRM RTKMHRGSRK GQGASFVASD SHVKLRIKSF 300
RVPELFIEIP ETATVGSLKR MVMEAVSTLL SDGHRVGLMV QGKKVRDDNK 350
TLHQTGISQD NSHLDSLDFS LEPSSEMPQL LTSHPLGHAC EELLPVCQAT 400
KIDNVLESDH HDSALFPSDS LGNNNVTEDS KAMISVALNE LSSQSQPPSR 450
KSRRSEQQQQ QAAQRRIRRP FSVAEVEALV QAVEKLGTGR WRDVKLCAFE 500
DADHRTYVDL KDKWKTLVHT AKISPQQRRG EPVPQELLNR VLNAHGYWTQ 550
QQMQQLQQNV NKLEQETQSQ TTEGLLLL 578
Length:578
Mass (Da):65,036
Last modified:May 18, 2010 - v2
Checksum:i853B54C5FC2E5E01
GO
Isoform 2 (identifier: Q8L7L8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     555-578: QLQQNVNKLEQETQSQTTEGLLLL → PWRSSLLCVLLLLLMIM

Note: No experimental confirmation available.

Show »
Length:571
Mass (Da):64,282
Checksum:i17BCF837A70A75FE
GO
Isoform 3 (identifier: Q8L7L8-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     555-578: QLQQNVNKLEQETQSQTTEGLLLL → HLGGRLSFVYYYYY

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:568
Mass (Da):64,081
Checksum:i88CA324D8E8DDC3D
GO
Isoform 4 (identifier: Q8L7L8-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     29-29: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:577
Mass (Da):64,908
Checksum:i6CF814FEE16B7055
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei29 – 291Missing in isoform 4.
VSP_039133
Alternative sequencei555 – 57824QLQQN…GLLLL → PWRSSLLCVLLLLLMIM in isoform 2.
VSP_039134Add
BLAST
Alternative sequencei555 – 57824QLQQN…GLLLL → HLGGRLSFVYYYYY in isoform 3.
VSP_039135Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41H → R in CAB50690. 1 Publication
Sequence conflicti58 – 581S → P in CAB50690. 1 Publication
Sequence conflicti137 – 1371D → E in BAH19498. 1 Publication
Sequence conflicti473 – 4731V → A in AAM91585. 1 Publication
Sequence conflicti473 – 4731V → A in AAN15436. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y17722 Genomic DNA. Translation: CAB50690.1.
AY519544 mRNA. Translation: AAS10014.1.
AB025604 Genomic DNA. Translation: BAA97479.1.
CP002688 Genomic DNA. Translation: AED97184.1.
CP002688 Genomic DNA. Translation: AED97185.1.
CP002688 Genomic DNA. Translation: AED97186.1.
CP002688 Genomic DNA. Translation: AED97187.1.
AY128382 mRNA. Translation: AAM91585.1.
BT000117 mRNA. Translation: AAN15436.1.
AK316779 mRNA. Translation: BAH19498.1.
AK318830 mRNA. Translation: BAH56945.1.
PIRiT51230.
RefSeqiNP_001078770.1. NM_001085301.1. [Q8L7L8-3]
NP_001078771.1. NM_001085302.1. [Q8L7L8-4]
NP_200751.1. NM_125334.3. [Q8L7L8-1]
NP_851221.1. NM_180890.1. [Q8L7L8-1]
UniGeneiAt.42990.

Genome annotation databases

EnsemblPlantsiAT5G59430.1; AT5G59430.1; AT5G59430. [Q8L7L8-1]
AT5G59430.2; AT5G59430.2; AT5G59430. [Q8L7L8-1]
GeneIDi836062.
KEGGiath:AT5G59430.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y17722 Genomic DNA. Translation: CAB50690.1 .
AY519544 mRNA. Translation: AAS10014.1 .
AB025604 Genomic DNA. Translation: BAA97479.1 .
CP002688 Genomic DNA. Translation: AED97184.1 .
CP002688 Genomic DNA. Translation: AED97185.1 .
CP002688 Genomic DNA. Translation: AED97186.1 .
CP002688 Genomic DNA. Translation: AED97187.1 .
AY128382 mRNA. Translation: AAM91585.1 .
BT000117 mRNA. Translation: AAN15436.1 .
AK316779 mRNA. Translation: BAH19498.1 .
AK318830 mRNA. Translation: BAH56945.1 .
PIRi T51230.
RefSeqi NP_001078770.1. NM_001085301.1. [Q8L7L8-3 ]
NP_001078771.1. NM_001085302.1. [Q8L7L8-4 ]
NP_200751.1. NM_125334.3. [Q8L7L8-1 ]
NP_851221.1. NM_180890.1. [Q8L7L8-1 ]
UniGenei At.42990.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2AJE NMR - A 464-560 [» ]
ProteinModelPortali Q8L7L8.
SMRi Q8L7L8. Positions 439-560.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8L7L8. 4 interactions.
MINTi MINT-1212501.
STRINGi 3702.AT5G59430.2-P.

Proteomic databases

PRIDEi Q8L7L8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G59430.1 ; AT5G59430.1 ; AT5G59430 . [Q8L7L8-1 ]
AT5G59430.2 ; AT5G59430.2 ; AT5G59430 . [Q8L7L8-1 ]
GeneIDi 836062.
KEGGi ath:AT5G59430.

Organism-specific databases

TAIRi AT5G59430.

Phylogenomic databases

eggNOGi NOG283818.
InParanoidi Q9LTI6.
OMAi CAFEDAD.
PhylomeDBi Q8L7L8.

Miscellaneous databases

EvolutionaryTracei Q8L7L8.

Gene expression databases

Genevestigatori Q8L7L8.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
SMARTi SM00717. SANT. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEi PS51294. HTH_MYB. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A plant gene encoding a Myb-like protein that binds telomeric GGTTTAG repeats in vitro."
    Chen C.M., Wang C.T., Ho C.H.
    J. Biol. Chem. 276:16511-16519(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DNA-BINDING.
  2. "The MYB transcription factor family in Arabidopsis: a genome-wide cloning and expression pattern analysis."
    Qu L., Gu H.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
    Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: cv. Columbia.
  7. "Interactions of putative telomere-binding proteins in Arabidopsis thaliana: identification of functional TRF2 homolog in plants."
    Kuchar M., Fajkus J.
    FEBS Lett. 578:311-315(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KU70.
  8. "A C-terminal Myb extension domain defines a novel family of double-strand telomeric DNA-binding proteins in Arabidopsis."
    Karamysheva Z.N., Surovtseva Y.V., Vespa L., Shakirov E.V., Shippen D.E.
    J. Biol. Chem. 279:47799-47807(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, TISSUE SPECIFICITY, SUBUNIT, DISRUPTION PHENOTYPE.
  9. "AtTBP2 and AtTRP2 in Arabidopsis encode proteins that bind plant telomeric DNA and induce DNA bending in vitro."
    Hwang M.G., Kim K., Lee W.K., Cho M.H.
    Mol. Genet. Genomics 273:66-75(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING, TISSUE SPECIFICITY.
  10. "The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family."
    Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X., Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H., Qu L.-J.
    Plant Mol. Biol. 60:107-124(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  11. "Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain: a new fold with an additional C-terminal helix."
    Sue S.C., Hsiao H.H., Chung B.C., Cheng Y.H., Hsueh K.L., Chen C.M., Ho C.H., Huang T.H.
    J. Mol. Biol. 356:72-85(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 464-560, DNA-BINDING.

Entry informationi

Entry nameiTRP1_ARATH
AccessioniPrimary (citable) accession number: Q8L7L8
Secondary accession number(s): A8MQF8
, A8MQM5, B9DFI1, C0Z2L6, Q9LTI6, Q9XGN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: May 18, 2010
Last modified: June 11, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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