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Protein

Telomere repeat-binding protein 1

Gene

TRP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to the plant telomeric double-stranded DNA sequences 5'-GGTTTAG-3'. At least 4 repeats of telomeric sequences are required for binding. Induces DNA bending.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi491 – 518H-T-H motifPROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

  • double-stranded telomeric DNA binding Source: TAIR

GO - Biological processi

  • ethylene-activated signaling pathway Source: TAIR
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to auxin Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to ethylene Source: TAIR
  • response to gibberellin Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to salicylic acid Source: TAIR
  • response to salt stress Source: TAIR
  • telomeric loop formation Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Telomere repeat-binding protein 1
Short name:
AtTRP1
Gene namesi
Name:TRP1
Ordered Locus Names:At5g59430
ORF Names:F2O15.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G59430.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • membrane Source: TAIR
  • nuclear membrane Source: TAIR
  • nuclear telomere cap complex Source: GO_Central
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, probably due to redundancy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003941241 – 578Telomere repeat-binding protein 1Add BLAST578

Proteomic databases

PaxDbiQ8L7L8.

Expressioni

Tissue specificityi

Expressed ubiquitously. Highest expression in flowers and leaves.2 Publications

Gene expression databases

GenevisibleiQ8L7L8. AT.

Interactioni

Subunit structurei

Homodimer and heterodimer with TRP2 and TRP3. Interacts with KU70.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ERS1Q388462EBI-476071,EBI-1606754
KU70Q9FQ082EBI-476071,EBI-476083

Protein-protein interaction databases

BioGridi21306. 1 interactor.
IntActiQ8L7L8. 4 interactors.
MINTiMINT-1212501.
STRINGi3702.AT5G59430.1.

Structurei

Secondary structure

1578
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi473 – 486Combined sources14
Beta strandi488 – 490Combined sources3
Helixi491 – 495Combined sources5
Beta strandi496 – 501Combined sources6
Helixi507 – 519Combined sources13
Turni525 – 529Combined sources5
Helixi536 – 551Combined sources16
Turni552 – 555Combined sources4
Beta strandi556 – 558Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AJENMR-A464-560[»]
ProteinModelPortaliQ8L7L8.
SMRiQ8L7L8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8L7L8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini293 – 372Ubiquitin-likePROSITE-ProRule annotationAdd BLAST80
Domaini463 – 522HTH myb-typePROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni465 – 469Interaction with DNA5
Regioni511 – 515Interaction with DNA5
Regioni522 – 529Interaction with DNA8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi457 – 461Poly-Gln5
Compositional biasi575 – 578Poly-Leu4

Domaini

A N-terminal domain (80-269) is responsible for the interaction with KU70.
The C-terminal domain (464-578) is sufficient for telomere binding.

Sequence similaritiesi

Contains 1 HTH myb-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410JUQM. Eukaryota.
ENOG41106NA. LUCA.
InParanoidiQ8L7L8.
OMAiSHKCVEE.
OrthoDBiEOG093609IS.
PhylomeDBiQ8L7L8.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51294. HTH_MYB. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8L7L8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSHKCVEEF GYASYLVPSN ARAPRSARKR RSIEKRISKE DDNMCAIDLL
60 70 80 90 100
ATVAGHLSFE SGSSLMSIDK LIEDHRVKEE FPEEEKPLMP VALSPYRGSL
110 120 130 140 150
SPCGFSSVIN GKVENEVDGF SYSGGSDACQ VGNFSQDVKP DIDGDAVVLD
160 170 180 190 200
ARPNVVVSLG SSSRTEVPSI GNCVSHGVRD DVNLFSRDDD ENFSKYIHPR
210 220 230 240 250
VTKHSPRTVP RIGDRRIRKI LASRHWKGGS RHSDTKPWRN YYLHQQRSYP
260 270 280 290 300
IKKRKNFDHI SDSVTDDYRM RTKMHRGSRK GQGASFVASD SHVKLRIKSF
310 320 330 340 350
RVPELFIEIP ETATVGSLKR MVMEAVSTLL SDGHRVGLMV QGKKVRDDNK
360 370 380 390 400
TLHQTGISQD NSHLDSLDFS LEPSSEMPQL LTSHPLGHAC EELLPVCQAT
410 420 430 440 450
KIDNVLESDH HDSALFPSDS LGNNNVTEDS KAMISVALNE LSSQSQPPSR
460 470 480 490 500
KSRRSEQQQQ QAAQRRIRRP FSVAEVEALV QAVEKLGTGR WRDVKLCAFE
510 520 530 540 550
DADHRTYVDL KDKWKTLVHT AKISPQQRRG EPVPQELLNR VLNAHGYWTQ
560 570
QQMQQLQQNV NKLEQETQSQ TTEGLLLL
Length:578
Mass (Da):65,036
Last modified:May 18, 2010 - v2
Checksum:i853B54C5FC2E5E01
GO
Isoform 2 (identifier: Q8L7L8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     555-578: QLQQNVNKLEQETQSQTTEGLLLL → PWRSSLLCVLLLLLMIM

Note: No experimental confirmation available.
Show »
Length:571
Mass (Da):64,282
Checksum:i17BCF837A70A75FE
GO
Isoform 3 (identifier: Q8L7L8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     555-578: QLQQNVNKLEQETQSQTTEGLLLL → HLGGRLSFVYYYYY

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:568
Mass (Da):64,081
Checksum:i88CA324D8E8DDC3D
GO
Isoform 4 (identifier: Q8L7L8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     29-29: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:577
Mass (Da):64,908
Checksum:i6CF814FEE16B7055
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4H → R in CAB50690 (PubMed:11278537).Curated1
Sequence conflicti58S → P in CAB50690 (PubMed:11278537).Curated1
Sequence conflicti137D → E in BAH19498 (PubMed:19423640).Curated1
Sequence conflicti473V → A in AAM91585 (PubMed:14593172).Curated1
Sequence conflicti473V → A in AAN15436 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03913329Missing in isoform 4. Curated1
Alternative sequenceiVSP_039134555 – 578QLQQN…GLLLL → PWRSSLLCVLLLLLMIM in isoform 2. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_039135555 – 578QLQQN…GLLLL → HLGGRLSFVYYYYY in isoform 3. CuratedAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17722 Genomic DNA. Translation: CAB50690.1.
AY519544 mRNA. Translation: AAS10014.1.
AB025604 Genomic DNA. Translation: BAA97479.1.
CP002688 Genomic DNA. Translation: AED97184.1.
CP002688 Genomic DNA. Translation: AED97185.1.
CP002688 Genomic DNA. Translation: AED97186.1.
CP002688 Genomic DNA. Translation: AED97187.1.
AY128382 mRNA. Translation: AAM91585.1.
BT000117 mRNA. Translation: AAN15436.1.
AK316779 mRNA. Translation: BAH19498.1.
AK318830 mRNA. Translation: BAH56945.1.
PIRiT51230.
RefSeqiNP_001078770.1. NM_001085301.1. [Q8L7L8-3]
NP_001078771.1. NM_001085302.1. [Q8L7L8-4]
NP_200751.1. NM_125334.4. [Q8L7L8-1]
NP_851221.1. NM_180890.1. [Q8L7L8-1]
UniGeneiAt.42990.

Genome annotation databases

EnsemblPlantsiAT5G59430.1; AT5G59430.1; AT5G59430. [Q8L7L8-1]
AT5G59430.2; AT5G59430.2; AT5G59430. [Q8L7L8-1]
GeneIDi836062.
GrameneiAT5G59430.1; AT5G59430.1; AT5G59430.
AT5G59430.2; AT5G59430.2; AT5G59430.
KEGGiath:AT5G59430.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17722 Genomic DNA. Translation: CAB50690.1.
AY519544 mRNA. Translation: AAS10014.1.
AB025604 Genomic DNA. Translation: BAA97479.1.
CP002688 Genomic DNA. Translation: AED97184.1.
CP002688 Genomic DNA. Translation: AED97185.1.
CP002688 Genomic DNA. Translation: AED97186.1.
CP002688 Genomic DNA. Translation: AED97187.1.
AY128382 mRNA. Translation: AAM91585.1.
BT000117 mRNA. Translation: AAN15436.1.
AK316779 mRNA. Translation: BAH19498.1.
AK318830 mRNA. Translation: BAH56945.1.
PIRiT51230.
RefSeqiNP_001078770.1. NM_001085301.1. [Q8L7L8-3]
NP_001078771.1. NM_001085302.1. [Q8L7L8-4]
NP_200751.1. NM_125334.4. [Q8L7L8-1]
NP_851221.1. NM_180890.1. [Q8L7L8-1]
UniGeneiAt.42990.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AJENMR-A464-560[»]
ProteinModelPortaliQ8L7L8.
SMRiQ8L7L8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21306. 1 interactor.
IntActiQ8L7L8. 4 interactors.
MINTiMINT-1212501.
STRINGi3702.AT5G59430.1.

Proteomic databases

PaxDbiQ8L7L8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G59430.1; AT5G59430.1; AT5G59430. [Q8L7L8-1]
AT5G59430.2; AT5G59430.2; AT5G59430. [Q8L7L8-1]
GeneIDi836062.
GrameneiAT5G59430.1; AT5G59430.1; AT5G59430.
AT5G59430.2; AT5G59430.2; AT5G59430.
KEGGiath:AT5G59430.

Organism-specific databases

TAIRiAT5G59430.

Phylogenomic databases

eggNOGiENOG410JUQM. Eukaryota.
ENOG41106NA. LUCA.
InParanoidiQ8L7L8.
OMAiSHKCVEE.
OrthoDBiEOG093609IS.
PhylomeDBiQ8L7L8.

Miscellaneous databases

EvolutionaryTraceiQ8L7L8.
PROiQ8L7L8.

Gene expression databases

GenevisibleiQ8L7L8. AT.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51294. HTH_MYB. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRP1_ARATH
AccessioniPrimary (citable) accession number: Q8L7L8
Secondary accession number(s): A8MQF8
, A8MQM5, B9DFI1, C0Z2L6, Q9LTI6, Q9XGN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.