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Q8L7L8

- TRP1_ARATH

UniProt

Q8L7L8 - TRP1_ARATH

Protein

Telomere repeat-binding protein 1

Gene

TRP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (18 May 2010)
      Previous versions | rss
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    Functioni

    Binds specifically to the plant telomeric double-stranded DNA sequences 5'-GGTTTAG-3'. At least 4 repeats of telomeric sequences are required for binding. Induces DNA bending.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi491 – 51828H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: InterPro
    2. double-stranded telomeric DNA binding Source: TAIR
    3. protein binding Source: IntAct

    GO - Biological processi

    1. ethylene-activated signaling pathway Source: TAIR
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. response to auxin Source: TAIR
    4. response to cadmium ion Source: TAIR
    5. response to ethylene Source: TAIR
    6. response to gibberellin Source: TAIR
    7. response to jasmonic acid Source: TAIR
    8. response to salicylic acid Source: TAIR
    9. response to salt stress Source: TAIR
    10. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Telomere repeat-binding protein 1
    Short name:
    AtTRP1
    Gene namesi
    Name:TRP1
    Ordered Locus Names:At5g59430
    ORF Names:F2O15.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G59430.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: TAIR
    2. membrane Source: TAIR
    3. nuclear membrane Source: TAIR
    4. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype, probably due to redundancy.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 578578Telomere repeat-binding protein 1PRO_0000394124Add
    BLAST

    Proteomic databases

    PRIDEiQ8L7L8.

    Expressioni

    Tissue specificityi

    Expressed ubiquitously. Highest expression in flowers and leaves.2 Publications

    Gene expression databases

    GenevestigatoriQ8L7L8.

    Interactioni

    Subunit structurei

    Homodimer and heterodimer with TRP2 and TRP3. Interacts with KU70.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ERS1Q388462EBI-476071,EBI-1606754
    KU70Q9FQ082EBI-476071,EBI-476083

    Protein-protein interaction databases

    IntActiQ8L7L8. 4 interactions.
    MINTiMINT-1212501.
    STRINGi3702.AT5G59430.2-P.

    Structurei

    Secondary structure

    1
    578
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi473 – 48614
    Beta strandi488 – 4903
    Helixi491 – 4955
    Beta strandi496 – 5016
    Helixi507 – 51913
    Turni525 – 5295
    Helixi536 – 55116
    Turni552 – 5554
    Beta strandi556 – 5583

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AJENMR-A464-560[»]
    ProteinModelPortaliQ8L7L8.
    SMRiQ8L7L8. Positions 439-560.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8L7L8.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini293 – 37280Ubiquitin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini463 – 52260HTH myb-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni465 – 4695Interaction with DNA
    Regioni511 – 5155Interaction with DNA
    Regioni522 – 5298Interaction with DNA

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi457 – 4615Poly-Gln
    Compositional biasi575 – 5784Poly-Leu

    Domaini

    A N-terminal domain (80-269) is responsible for the interaction with KU70.
    The C-terminal domain (464-578) is sufficient for telomere binding.

    Sequence similaritiesi

    Contains 1 HTH myb-type DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG283818.
    InParanoidiQ9LTI6.
    OMAiCAFEDAD.
    PhylomeDBiQ8L7L8.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR009057. Homeodomain-like.
    IPR017930. Myb_dom.
    IPR001005. SANT/Myb.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    SMARTiSM00717. SANT. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS51294. HTH_MYB. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8L7L8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVSHKCVEEF GYASYLVPSN ARAPRSARKR RSIEKRISKE DDNMCAIDLL    50
    ATVAGHLSFE SGSSLMSIDK LIEDHRVKEE FPEEEKPLMP VALSPYRGSL 100
    SPCGFSSVIN GKVENEVDGF SYSGGSDACQ VGNFSQDVKP DIDGDAVVLD 150
    ARPNVVVSLG SSSRTEVPSI GNCVSHGVRD DVNLFSRDDD ENFSKYIHPR 200
    VTKHSPRTVP RIGDRRIRKI LASRHWKGGS RHSDTKPWRN YYLHQQRSYP 250
    IKKRKNFDHI SDSVTDDYRM RTKMHRGSRK GQGASFVASD SHVKLRIKSF 300
    RVPELFIEIP ETATVGSLKR MVMEAVSTLL SDGHRVGLMV QGKKVRDDNK 350
    TLHQTGISQD NSHLDSLDFS LEPSSEMPQL LTSHPLGHAC EELLPVCQAT 400
    KIDNVLESDH HDSALFPSDS LGNNNVTEDS KAMISVALNE LSSQSQPPSR 450
    KSRRSEQQQQ QAAQRRIRRP FSVAEVEALV QAVEKLGTGR WRDVKLCAFE 500
    DADHRTYVDL KDKWKTLVHT AKISPQQRRG EPVPQELLNR VLNAHGYWTQ 550
    QQMQQLQQNV NKLEQETQSQ TTEGLLLL 578
    Length:578
    Mass (Da):65,036
    Last modified:May 18, 2010 - v2
    Checksum:i853B54C5FC2E5E01
    GO
    Isoform 2 (identifier: Q8L7L8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         555-578: QLQQNVNKLEQETQSQTTEGLLLL → PWRSSLLCVLLLLLMIM

    Note: No experimental confirmation available.

    Show »
    Length:571
    Mass (Da):64,282
    Checksum:i17BCF837A70A75FE
    GO
    Isoform 3 (identifier: Q8L7L8-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         555-578: QLQQNVNKLEQETQSQTTEGLLLL → HLGGRLSFVYYYYY

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:568
    Mass (Da):64,081
    Checksum:i88CA324D8E8DDC3D
    GO
    Isoform 4 (identifier: Q8L7L8-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         29-29: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:577
    Mass (Da):64,908
    Checksum:i6CF814FEE16B7055
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41H → R in CAB50690. (PubMed:11278537)Curated
    Sequence conflicti58 – 581S → P in CAB50690. (PubMed:11278537)Curated
    Sequence conflicti137 – 1371D → E in BAH19498. (PubMed:19423640)Curated
    Sequence conflicti473 – 4731V → A in AAM91585. (PubMed:14593172)Curated
    Sequence conflicti473 – 4731V → A in AAN15436. (PubMed:14593172)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei29 – 291Missing in isoform 4. CuratedVSP_039133
    Alternative sequencei555 – 57824QLQQN…GLLLL → PWRSSLLCVLLLLLMIM in isoform 2. 1 PublicationVSP_039134Add
    BLAST
    Alternative sequencei555 – 57824QLQQN…GLLLL → HLGGRLSFVYYYYY in isoform 3. CuratedVSP_039135Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y17722 Genomic DNA. Translation: CAB50690.1.
    AY519544 mRNA. Translation: AAS10014.1.
    AB025604 Genomic DNA. Translation: BAA97479.1.
    CP002688 Genomic DNA. Translation: AED97184.1.
    CP002688 Genomic DNA. Translation: AED97185.1.
    CP002688 Genomic DNA. Translation: AED97186.1.
    CP002688 Genomic DNA. Translation: AED97187.1.
    AY128382 mRNA. Translation: AAM91585.1.
    BT000117 mRNA. Translation: AAN15436.1.
    AK316779 mRNA. Translation: BAH19498.1.
    AK318830 mRNA. Translation: BAH56945.1.
    PIRiT51230.
    RefSeqiNP_001078770.1. NM_001085301.1. [Q8L7L8-3]
    NP_001078771.1. NM_001085302.1. [Q8L7L8-4]
    NP_200751.1. NM_125334.3. [Q8L7L8-1]
    NP_851221.1. NM_180890.1. [Q8L7L8-1]
    UniGeneiAt.42990.

    Genome annotation databases

    EnsemblPlantsiAT5G59430.1; AT5G59430.1; AT5G59430. [Q8L7L8-1]
    AT5G59430.2; AT5G59430.2; AT5G59430. [Q8L7L8-1]
    GeneIDi836062.
    KEGGiath:AT5G59430.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y17722 Genomic DNA. Translation: CAB50690.1 .
    AY519544 mRNA. Translation: AAS10014.1 .
    AB025604 Genomic DNA. Translation: BAA97479.1 .
    CP002688 Genomic DNA. Translation: AED97184.1 .
    CP002688 Genomic DNA. Translation: AED97185.1 .
    CP002688 Genomic DNA. Translation: AED97186.1 .
    CP002688 Genomic DNA. Translation: AED97187.1 .
    AY128382 mRNA. Translation: AAM91585.1 .
    BT000117 mRNA. Translation: AAN15436.1 .
    AK316779 mRNA. Translation: BAH19498.1 .
    AK318830 mRNA. Translation: BAH56945.1 .
    PIRi T51230.
    RefSeqi NP_001078770.1. NM_001085301.1. [Q8L7L8-3 ]
    NP_001078771.1. NM_001085302.1. [Q8L7L8-4 ]
    NP_200751.1. NM_125334.3. [Q8L7L8-1 ]
    NP_851221.1. NM_180890.1. [Q8L7L8-1 ]
    UniGenei At.42990.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2AJE NMR - A 464-560 [» ]
    ProteinModelPortali Q8L7L8.
    SMRi Q8L7L8. Positions 439-560.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8L7L8. 4 interactions.
    MINTi MINT-1212501.
    STRINGi 3702.AT5G59430.2-P.

    Proteomic databases

    PRIDEi Q8L7L8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G59430.1 ; AT5G59430.1 ; AT5G59430 . [Q8L7L8-1 ]
    AT5G59430.2 ; AT5G59430.2 ; AT5G59430 . [Q8L7L8-1 ]
    GeneIDi 836062.
    KEGGi ath:AT5G59430.

    Organism-specific databases

    TAIRi AT5G59430.

    Phylogenomic databases

    eggNOGi NOG283818.
    InParanoidi Q9LTI6.
    OMAi CAFEDAD.
    PhylomeDBi Q8L7L8.

    Miscellaneous databases

    EvolutionaryTracei Q8L7L8.

    Gene expression databases

    Genevestigatori Q8L7L8.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR009057. Homeodomain-like.
    IPR017930. Myb_dom.
    IPR001005. SANT/Myb.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    SMARTi SM00717. SANT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS51294. HTH_MYB. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A plant gene encoding a Myb-like protein that binds telomeric GGTTTAG repeats in vitro."
      Chen C.M., Wang C.T., Ho C.H.
      J. Biol. Chem. 276:16511-16519(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DNA-BINDING.
    2. "The MYB transcription factor family in Arabidopsis: a genome-wide cloning and expression pattern analysis."
      Qu L., Gu H.
      Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
      Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    6. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: cv. Columbia.
    7. "Interactions of putative telomere-binding proteins in Arabidopsis thaliana: identification of functional TRF2 homolog in plants."
      Kuchar M., Fajkus J.
      FEBS Lett. 578:311-315(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KU70.
    8. "A C-terminal Myb extension domain defines a novel family of double-strand telomeric DNA-binding proteins in Arabidopsis."
      Karamysheva Z.N., Surovtseva Y.V., Vespa L., Shakirov E.V., Shippen D.E.
      J. Biol. Chem. 279:47799-47807(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, TISSUE SPECIFICITY, SUBUNIT, DISRUPTION PHENOTYPE.
    9. "AtTBP2 and AtTRP2 in Arabidopsis encode proteins that bind plant telomeric DNA and induce DNA bending in vitro."
      Hwang M.G., Kim K., Lee W.K., Cho M.H.
      Mol. Genet. Genomics 273:66-75(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING, TISSUE SPECIFICITY.
    10. "The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family."
      Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X., Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H., Qu L.-J.
      Plant Mol. Biol. 60:107-124(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    11. "Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain: a new fold with an additional C-terminal helix."
      Sue S.C., Hsiao H.H., Chung B.C., Cheng Y.H., Hsueh K.L., Chen C.M., Ho C.H., Huang T.H.
      J. Mol. Biol. 356:72-85(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 464-560, DNA-BINDING.

    Entry informationi

    Entry nameiTRP1_ARATH
    AccessioniPrimary (citable) accession number: Q8L7L8
    Secondary accession number(s): A8MQF8
    , A8MQM5, B9DFI1, C0Z2L6, Q9LTI6, Q9XGN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 18, 2010
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3