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Q8L7J2

- BGL06_ORYSJ

UniProt

Q8L7J2 - BGL06_ORYSJ

Protein

Beta-glucosidase 6

Gene

BGLU6

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Hydrolyzes glycosides, oligosaccharides and hydrophobic glycosides.1 Publication

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Kineticsi

    1. KM=6.3 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0)1 Publication
    2. KM=0.5 mM for p-nitrophenyl beta-D-fucoside (at pH 5.0)1 Publication
    3. KM=6.06 mM for p-nitrophenyl beta-D-galactoside (at pH 5.0)1 Publication
    4. KM=4.5 mM for n-octyl-beta-D-glucoside (at pH 5.0)1 Publication
    5. KM=5.0 mM for n-heptyl-beta-D-glucoside (at pH 5.0)1 Publication
    6. KM=3.6 mM for laminaribiose (at pH 5.0)1 Publication
    7. KM=8.7 mM for laminaritriose (at pH 5.0)1 Publication
    8. KM=15.3 mM for cellobiose (at pH 5.0)1 Publication
    9. KM=9.8 mM for sophorose (at pH 5.0)1 Publication
    10. KM=14.9 mM for gentiobiose (at pH 5.0)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei64 – 641Substrate
    Metal bindingi100 – 1001Zinc; shared with dimeric partnerSequence Analysis
    Metal bindingi103 – 1031Zinc; shared with dimeric partnerSequence Analysis
    Binding sitei165 – 1651Substrate
    Binding sitei210 – 2101Substrate
    Active sitei211 – 2111Proton donorBy similarity
    Binding sitei354 – 3541Substrate
    Active sitei427 – 4271NucleophileBy similarity
    Binding sitei477 – 4771Substrate

    GO - Molecular functioni

    1. beta-D-fucosidase activity Source: UniProtKB
    2. beta-galactosidase activity Source: UniProtKB
    3. beta-gentiobiose beta-glucosidase activity Source: UniProtKB
    4. beta-glucosidase activity Source: UniProtKB
    5. cellobiose glucosidase activity Source: UniProtKB
    6. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB
    7. hydrolase activity, acting on glycosyl bonds Source: UniProtKB
    8. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKQ8L7J2.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 6 (EC:3.2.1.21)
    Short name:
    Os3bglu6
    Gene namesi
    Name:BGLU6
    Ordered Locus Names:Os03g0212800, LOC_Os03g11420
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 3

    Organism-specific databases

    GrameneiQ8L7J2.

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 521490Beta-glucosidase 6PRO_0000387495Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi230 ↔ 2381 Publication
    Glycosylationi291 – 2911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi372 – 3721N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ8L7J2.

    Interactioni

    Subunit structurei

    Homodimer.Curated

    Structurei

    Secondary structure

    1
    521
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi47 – 493
    Beta strandi55 – 595
    Helixi62 – 654
    Helixi71 – 733
    Helixi78 – 836
    Helixi101 – 11515
    Beta strandi119 – 1235
    Helixi126 – 1294
    Beta strandi133 – 1364
    Helixi139 – 15416
    Beta strandi158 – 1669
    Helixi170 – 1767
    Helixi178 – 1803
    Helixi183 – 19917
    Turni200 – 2023
    Beta strandi205 – 2106
    Helixi212 – 2209
    Turni232 – 2343
    Turni243 – 2453
    Helixi246 – 26823
    Helixi270 – 2734
    Beta strandi276 – 2827
    Beta strandi285 – 2928
    Helixi293 – 30614
    Helixi308 – 3169
    Helixi321 – 3277
    Helixi328 – 3303
    Helixi336 – 3427
    Beta strandi347 – 3526
    Beta strandi356 – 3616
    Beta strandi366 – 3683
    Helixi369 – 3713
    Helixi374 – 3774
    Beta strandi380 – 3867
    Beta strandi389 – 3924
    Helixi405 – 41814
    Beta strandi423 – 4286
    Helixi440 – 4434
    Helixi447 – 46519
    Beta strandi471 – 4777
    Helixi485 – 4906
    Beta strandi495 – 4984
    Turni500 – 5045
    Beta strandi506 – 5083
    Helixi510 – 51910

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3GNOX-ray1.83A38-521[»]
    3GNPX-ray1.80A38-521[»]
    3GNRX-ray1.81A38-521[»]
    3WBAX-ray1.90A38-521[»]
    3WBEX-ray1.97A38-521[»]
    ProteinModelPortaliQ8L7J2.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8L7J2.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni484 – 4852Substrate binding

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    KOiK01188.
    OMAiQIEGAPE.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8L7J2-1 [UniParc]FASTAAdd to Basket

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    MGRIKSSSGR CSTARLEAVA VLVVVFGVAS SSLRGCIAQQ SGGGLTRGSF    50
    PEGFVFGTAS AAYQYEGAVK EDGRGQTIWD TFAHTFGKIT DFSNADVAVD 100
    QYHRFEEDIQ LMADMGMDAY RFSIAWSRIY PNGVGQVNQA GIDHYNKLID 150
    ALLAKGIQPY VTLYHWDLPQ ALEDKYKGWL DRQIVDDFAA YAETCFREFG 200
    DRVKHWITLN EPHTVAIQGY DAGLQAPGRC SVLLHLYCKA GNSGTEPYVV 250
    AHHFILAHAA AASIYRTKYK ATQNGQLGIA FDVMWFEPMS NTTIDIEAAK 300
    RAQEFQLGWF ADPFFFGDYP ATMRARVGER LPRFTADEAA VVKGALDFVG 350
    INHYTTYYTR HNNTNIIGTL LNNTLADTGT VSLPFKNGKP IGDRANSIWL 400
    YIVPRGMRSL MNYVKERYNS PPVYITENGM DDSNNPFISI KDALKDSKRI 450
    KYHNDYLTNL AASIKEDGCD VRGYFAWSLL DNWEWAAGYS SRFGLYFVDY 500
    KDNLKRYPKN SVQWFKALLK T 521
    Length:521
    Mass (Da):58,539
    Last modified:October 1, 2002 - v1
    Checksum:i0554435869F3BD21
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY129294 mRNA. Translation: AAN01354.1.
    DP000009 Genomic DNA. Translation: ABF94615.1.
    AP008209 Genomic DNA. Translation: BAF11272.1.
    AK119546 mRNA. Translation: BAG99682.1.
    RefSeqiNP_001049358.1. NM_001055893.1.
    UniGeneiOs.15799.

    Genome annotation databases

    EnsemblPlantsiOS03T0212800-01; OS03T0212800-01; OS03G0212800.
    GeneIDi4332041.
    KEGGiosa:4332041.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY129294 mRNA. Translation: AAN01354.1 .
    DP000009 Genomic DNA. Translation: ABF94615.1 .
    AP008209 Genomic DNA. Translation: BAF11272.1 .
    AK119546 mRNA. Translation: BAG99682.1 .
    RefSeqi NP_001049358.1. NM_001055893.1.
    UniGenei Os.15799.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3GNO X-ray 1.83 A 38-521 [» ]
    3GNP X-ray 1.80 A 38-521 [» ]
    3GNR X-ray 1.81 A 38-521 [» ]
    3WBA X-ray 1.90 A 38-521 [» ]
    3WBE X-ray 1.97 A 38-521 [» ]
    ProteinModelPortali Q8L7J2.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PRIDEi Q8L7J2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS03T0212800-01 ; OS03T0212800-01 ; OS03G0212800 .
    GeneIDi 4332041.
    KEGGi osa:4332041.

    Organism-specific databases

    Gramenei Q8L7J2.

    Phylogenomic databases

    eggNOGi COG2723.
    KOi K01188.
    OMAi QIEGAPE.

    Enzyme and pathway databases

    SABIO-RK Q8L7J2.

    Miscellaneous databases

    EvolutionaryTracei Q8L7J2.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural and enzymatic characterization of Os3BGlu6, a rice beta-glucosidase hydrolyzing hydrophobic glycosides and (1->3)- and (1->2)-linked disaccharides."
      Seshadri S., Akiyama T., Opassiri R., Kuaprasert B., Cairns J.K.
      Plant Physiol. 151:47-58(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 38-521 IN COMPLEX WITH SUBSTRATE ANALOG, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISULFIDE BONDS.
      Strain: cv. Yukihikari.
    2. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
      The rice chromosome 3 sequencing consortium
      Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
      , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
      Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.
    6. "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
      Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
      BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiBGL06_ORYSJ
    AccessioniPrimary (citable) accession number: Q8L7J2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 3, 2009
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3