Beta-glucosidase 6 precursor - Oryza sativa subsp. japonica (Rice)

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Reviewed, UniProtKB/Swiss-Prot Q8L7J2 (BGL06_ORYSJ)

Last modified November 24, 2009. Version 40. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Beta-glucosidase 6
      Short name=Os3bglu6
    EC=3.2.1.21

Gene names

Name:	BGLU6
Ordered Locus Names:	Os03g0212800, LOC_Os03g11420

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

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Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Hydrolyzes glycosides, oligosaccharides and hydrophobic glycosides. Ref.1
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Subunit structure	Homodimer Potential.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.
Biophysicochemical properties	Kinetic parameters: K_M=6.3 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0) K_M=0.5 mM for p-nitrophenyl beta-D-fucoside (at pH 5.0) K_M=6.06 mM for p-nitrophenyl beta-D-galactoside (at pH 5.0) K_M=4.5 mM for n-octyl-beta-D-glucoside (at pH 5.0) K_M=5.0 mM for n-heptyl-beta-D-glucoside (at pH 5.0) K_M=3.6 mM for laminaribiose (at pH 5.0) K_M=8.7 mM for laminaritriose (at pH 5.0) K_M=15.3 mM for cellobiose (at pH 5.0) K_M=9.8 mM for sophorose (at pH 5.0) K_M=14.9 mM for gentiobiose (at pH 5.0)

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Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-D-fucosidase activity Ref.1 Inferred from direct assay. Source: UniProtKB beta-galactosidase activity Ref.1 Inferred from direct assay. Source: UniProtKB beta-gentiobiose beta-glucosidase activity Ref.1 Inferred from direct assay. Source: UniProtKB beta-glucosidase activity Ref.1 Inferred from direct assay. Source: UniProtKB cation binding Inferred from electronic annotation. Source: InterPro cellobiose glucosidase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 31

Potential

Chain

32 – 521

490

Beta-glucosidase 6

PRO_0000387495

Sites

Active site

211

Proton donor By similarity

Active site

427

Nucleophile By similarity

Metal binding

100

Zinc; shared with dimeric partner Potential

Metal binding

103

Zinc; shared with dimeric partner Potential

Binding site

Substrate

Binding site

210

Substrate

Binding site

427

Substrate

Binding site

484

Substrate

Binding site

485

Substrate

Amino acid modifications

Glycosylation

291

N-linked (GlcNAc...) Potential

Glycosylation

362

N-linked (GlcNAc...) Potential

Glycosylation

372

N-linked (GlcNAc...) Potential

Disulfide bond

230 ↔ 238

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Sequences

Sequence

Length

Mass (Da)

Tools

Q8L7J2-1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 0554435869F3BD21
Show »

FASTA

521

58,539

        10         20         30         40         50         60 
MGRIKSSSGR CSTARLEAVA VLVVVFGVAS SSLRGCIAQQ SGGGLTRGSF PEGFVFGTAS 

        70         80         90        100        110        120 
AAYQYEGAVK EDGRGQTIWD TFAHTFGKIT DFSNADVAVD QYHRFEEDIQ LMADMGMDAY 

       130        140        150        160        170        180 
RFSIAWSRIY PNGVGQVNQA GIDHYNKLID ALLAKGIQPY VTLYHWDLPQ ALEDKYKGWL 

       190        200        210        220        230        240 
DRQIVDDFAA YAETCFREFG DRVKHWITLN EPHTVAIQGY DAGLQAPGRC SVLLHLYCKA 

       250        260        270        280        290        300 
GNSGTEPYVV AHHFILAHAA AASIYRTKYK ATQNGQLGIA FDVMWFEPMS NTTIDIEAAK 

       310        320        330        340        350        360 
RAQEFQLGWF ADPFFFGDYP ATMRARVGER LPRFTADEAA VVKGALDFVG INHYTTYYTR 

       370        380        390        400        410        420 
HNNTNIIGTL LNNTLADTGT VSLPFKNGKP IGDRANSIWL YIVPRGMRSL MNYVKERYNS 

       430        440        450        460        470        480 
PPVYITENGM DDSNNPFISI KDALKDSKRI KYHNDYLTNL AASIKEDGCD VRGYFAWSLL 

       490        500        510        520 
DNWEWAAGYS SRFGLYFVDY KDNLKRYPKN SVQWFKALLK T

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural and enzymatic characterization of Os3BGlu6, a rice beta-glucosidase hydrolyzing hydrophobic glycosides and (1->3)- and (1->2)-linked disaccharides." Seshadri S., Akiyama T., Opassiri R., Kuaprasert B., Cairns J.K. Plant Physiol. 151:47-58(2009) [PubMed: 19587102] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 38-521 IN COMPLEX WITH SUBSTRATE ANALOG, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISULFID BONDS. Strain: cv. Yukihikari.
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[6]	"Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase." Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R. BMC Plant Biol. 6:33-33(2006) [PubMed: 17196101] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

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Cross-references

Sequence databases

AY129294 mRNA. Translation: AAN01354.1.
DP000009 Genomic DNA. Translation: ABF94615.1.
AP008209 Genomic DNA. Translation: BAF11272.1.
AK119546 mRNA. Translation: BAG99682.1.

RefSeq

NP_001049358.1.

UniGene

Os.15799

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GNO	X-ray	1.83	A	38-521	[»]
3GNP	X-ray	1.80	A	38-521	[»]
3GNR	X-ray	1.81	A	38-521	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH1. Glycoside Hydrolase Family 1.

Genome annotation databases

GeneID

4332041.

GenomeReviews

Gene locus Os03g0212800 in contig AP008209_GR.

KEGG

osa:4332041.

Organism-specific databases

Gramene

Q8L7J2.

Phylogenomic databases

PhylomeDB

Q8L7J2.

Family and domain databases

InterPro

IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR017853. Glyco_hydro_catalytic_core.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

PANTHER

PTHR10353. Glyco_hydro_1. 1 hit.

Pfam

PF00232. Glyco_hydro_1. 1 hit.
[Graphical view]

PRINTS

PR00131. GLHYDRLASE1.

PROSITE

PS00572. GLYCOSYL_HYDROL_F1_1. False negative.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

BGL06_ORYSJ

Accession

Primary (citable) accession number: Q8L7J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	October 1, 2002
Last modified:	November 24, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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