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Q8L7J2

- BGL06_ORYSJ

UniProt

Q8L7J2 - BGL06_ORYSJ

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Protein

Beta-glucosidase 6

Gene

BGLU6

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes glycosides, oligosaccharides and hydrophobic glycosides.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=6.3 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0)1 Publication
  2. KM=0.5 mM for p-nitrophenyl beta-D-fucoside (at pH 5.0)1 Publication
  3. KM=6.06 mM for p-nitrophenyl beta-D-galactoside (at pH 5.0)1 Publication
  4. KM=4.5 mM for n-octyl-beta-D-glucoside (at pH 5.0)1 Publication
  5. KM=5.0 mM for n-heptyl-beta-D-glucoside (at pH 5.0)1 Publication
  6. KM=3.6 mM for laminaribiose (at pH 5.0)1 Publication
  7. KM=8.7 mM for laminaritriose (at pH 5.0)1 Publication
  8. KM=15.3 mM for cellobiose (at pH 5.0)1 Publication
  9. KM=9.8 mM for sophorose (at pH 5.0)1 Publication
  10. KM=14.9 mM for gentiobiose (at pH 5.0)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641Substrate
Metal bindingi100 – 1001Zinc; shared with dimeric partnerSequence Analysis
Metal bindingi103 – 1031Zinc; shared with dimeric partnerSequence Analysis
Binding sitei165 – 1651Substrate
Binding sitei210 – 2101Substrate
Active sitei211 – 2111Proton donorBy similarity
Binding sitei354 – 3541Substrate
Active sitei427 – 4271NucleophileBy similarity
Binding sitei477 – 4771Substrate

GO - Molecular functioni

  1. beta-D-fucosidase activity Source: UniProtKB
  2. beta-galactosidase activity Source: UniProtKB
  3. beta-gentiobiose beta-glucosidase activity Source: UniProtKB
  4. beta-glucosidase activity Source: UniProtKB
  5. cellobiose glucosidase activity Source: UniProtKB
  6. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB
  7. hydrolase activity, acting on glycosyl bonds Source: UniProtKB
  8. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKQ8L7J2.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 6 (EC:3.2.1.21)
Short name:
Os3bglu6
Gene namesi
Name:BGLU6
Ordered Locus Names:Os03g0212800, LOC_Os03g11420
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ8L7J2.

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 521490Beta-glucosidase 6PRO_0000387495Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi230 ↔ 2381 Publication
Glycosylationi291 – 2911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi372 – 3721N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ8L7J2.

Expressioni

Gene expression databases

ExpressionAtlasiQ8L7J2. baseline.

Interactioni

Subunit structurei

Homodimer.Curated

Structurei

Secondary structure

1
521
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi47 – 493
Beta strandi55 – 595
Helixi62 – 654
Helixi71 – 733
Helixi78 – 836
Helixi101 – 11515
Beta strandi119 – 1235
Helixi126 – 1294
Beta strandi133 – 1364
Helixi139 – 15416
Beta strandi158 – 1669
Helixi170 – 1767
Helixi178 – 1803
Helixi183 – 19917
Turni200 – 2023
Beta strandi205 – 2106
Helixi212 – 2209
Turni232 – 2343
Turni243 – 2453
Helixi246 – 26823
Helixi270 – 2734
Beta strandi276 – 2827
Beta strandi285 – 2928
Helixi293 – 30614
Helixi308 – 3169
Helixi321 – 3277
Helixi328 – 3303
Helixi336 – 3427
Beta strandi347 – 3526
Beta strandi356 – 3616
Beta strandi366 – 3683
Helixi369 – 3713
Helixi374 – 3774
Beta strandi380 – 3867
Beta strandi389 – 3924
Helixi405 – 41814
Beta strandi423 – 4286
Helixi440 – 4434
Helixi447 – 46519
Beta strandi471 – 4777
Helixi485 – 4906
Beta strandi495 – 4984
Turni500 – 5045
Beta strandi506 – 5083
Helixi510 – 51910

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GNOX-ray1.83A38-521[»]
3GNPX-ray1.80A38-521[»]
3GNRX-ray1.81A38-521[»]
3WBAX-ray1.90A38-521[»]
3WBEX-ray1.97A38-521[»]
ProteinModelPortaliQ8L7J2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8L7J2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni484 – 4852Substrate binding

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
InParanoidiQ8L7J2.
KOiK01188.
OMAiQIEGAPE.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L7J2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRIKSSSGR CSTARLEAVA VLVVVFGVAS SSLRGCIAQQ SGGGLTRGSF
60 70 80 90 100
PEGFVFGTAS AAYQYEGAVK EDGRGQTIWD TFAHTFGKIT DFSNADVAVD
110 120 130 140 150
QYHRFEEDIQ LMADMGMDAY RFSIAWSRIY PNGVGQVNQA GIDHYNKLID
160 170 180 190 200
ALLAKGIQPY VTLYHWDLPQ ALEDKYKGWL DRQIVDDFAA YAETCFREFG
210 220 230 240 250
DRVKHWITLN EPHTVAIQGY DAGLQAPGRC SVLLHLYCKA GNSGTEPYVV
260 270 280 290 300
AHHFILAHAA AASIYRTKYK ATQNGQLGIA FDVMWFEPMS NTTIDIEAAK
310 320 330 340 350
RAQEFQLGWF ADPFFFGDYP ATMRARVGER LPRFTADEAA VVKGALDFVG
360 370 380 390 400
INHYTTYYTR HNNTNIIGTL LNNTLADTGT VSLPFKNGKP IGDRANSIWL
410 420 430 440 450
YIVPRGMRSL MNYVKERYNS PPVYITENGM DDSNNPFISI KDALKDSKRI
460 470 480 490 500
KYHNDYLTNL AASIKEDGCD VRGYFAWSLL DNWEWAAGYS SRFGLYFVDY
510 520
KDNLKRYPKN SVQWFKALLK T
Length:521
Mass (Da):58,539
Last modified:October 1, 2002 - v1
Checksum:i0554435869F3BD21
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY129294 mRNA. Translation: AAN01354.1.
DP000009 Genomic DNA. Translation: ABF94615.1.
AP008209 Genomic DNA. Translation: BAF11272.1.
AK119546 mRNA. Translation: BAG99682.1.
RefSeqiNP_001049358.1. NM_001055893.1.
UniGeneiOs.15799.

Genome annotation databases

EnsemblPlantsiOS03T0212800-01; OS03T0212800-01; OS03G0212800.
GeneIDi4332041.
KEGGiosa:4332041.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY129294 mRNA. Translation: AAN01354.1 .
DP000009 Genomic DNA. Translation: ABF94615.1 .
AP008209 Genomic DNA. Translation: BAF11272.1 .
AK119546 mRNA. Translation: BAG99682.1 .
RefSeqi NP_001049358.1. NM_001055893.1.
UniGenei Os.15799.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3GNO X-ray 1.83 A 38-521 [» ]
3GNP X-ray 1.80 A 38-521 [» ]
3GNR X-ray 1.81 A 38-521 [» ]
3WBA X-ray 1.90 A 38-521 [» ]
3WBE X-ray 1.97 A 38-521 [» ]
ProteinModelPortali Q8L7J2.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEi Q8L7J2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS03T0212800-01 ; OS03T0212800-01 ; OS03G0212800 .
GeneIDi 4332041.
KEGGi osa:4332041.

Organism-specific databases

Gramenei Q8L7J2.

Phylogenomic databases

eggNOGi COG2723.
InParanoidi Q8L7J2.
KOi K01188.
OMAi QIEGAPE.

Enzyme and pathway databases

SABIO-RK Q8L7J2.

Miscellaneous databases

EvolutionaryTracei Q8L7J2.

Gene expression databases

ExpressionAtlasi Q8L7J2. baseline.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural and enzymatic characterization of Os3BGlu6, a rice beta-glucosidase hydrolyzing hydrophobic glycosides and (1->3)- and (1->2)-linked disaccharides."
    Seshadri S., Akiyama T., Opassiri R., Kuaprasert B., Cairns J.K.
    Plant Physiol. 151:47-58(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 38-521 IN COMPLEX WITH SUBSTRATE ANALOG, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISULFIDE BONDS.
    Strain: cv. Yukihikari.
  2. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  6. "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
    Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
    BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL06_ORYSJ
AccessioniPrimary (citable) accession number: Q8L7J2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3