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Q8L7J2 (BGL06_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 6

Short name=Os3bglu6
EC=3.2.1.21
Gene names
Name:BGLU6
Ordered Locus Names:Os03g0212800, LOC_Os03g11420
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length521 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes glycosides, oligosaccharides and hydrophobic glycosides. Ref.1

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Subunit structure

Homodimer Potential.

Subcellular location

Secreted Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Biophysicochemical properties

Kinetic parameters:

KM=6.3 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0) Ref.1

KM=0.5 mM for p-nitrophenyl beta-D-fucoside (at pH 5.0)

KM=6.06 mM for p-nitrophenyl beta-D-galactoside (at pH 5.0)

KM=4.5 mM for n-octyl-beta-D-glucoside (at pH 5.0)

KM=5.0 mM for n-heptyl-beta-D-glucoside (at pH 5.0)

KM=3.6 mM for laminaribiose (at pH 5.0)

KM=8.7 mM for laminaritriose (at pH 5.0)

KM=15.3 mM for cellobiose (at pH 5.0)

KM=9.8 mM for sophorose (at pH 5.0)

KM=14.9 mM for gentiobiose (at pH 5.0)

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 521490Beta-glucosidase 6
PRO_0000387495

Regions

Region484 – 4852Substrate binding

Sites

Active site2111Proton donor By similarity
Active site4271Nucleophile By similarity
Metal binding1001Zinc; shared with dimeric partner Potential
Metal binding1031Zinc; shared with dimeric partner Potential
Binding site641Substrate
Binding site1651Substrate
Binding site2101Substrate
Binding site3541Substrate
Binding site4771Substrate

Amino acid modifications

Glycosylation2911N-linked (GlcNAc...) Potential
Glycosylation3621N-linked (GlcNAc...) Potential
Glycosylation3721N-linked (GlcNAc...) Potential
Disulfide bond230 ↔ 238 Ref.1

Secondary structure

...................................................................................... 521
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8L7J2 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 0554435869F3BD21

FASTA52158,539
        10         20         30         40         50         60 
MGRIKSSSGR CSTARLEAVA VLVVVFGVAS SSLRGCIAQQ SGGGLTRGSF PEGFVFGTAS 

        70         80         90        100        110        120 
AAYQYEGAVK EDGRGQTIWD TFAHTFGKIT DFSNADVAVD QYHRFEEDIQ LMADMGMDAY 

       130        140        150        160        170        180 
RFSIAWSRIY PNGVGQVNQA GIDHYNKLID ALLAKGIQPY VTLYHWDLPQ ALEDKYKGWL 

       190        200        210        220        230        240 
DRQIVDDFAA YAETCFREFG DRVKHWITLN EPHTVAIQGY DAGLQAPGRC SVLLHLYCKA 

       250        260        270        280        290        300 
GNSGTEPYVV AHHFILAHAA AASIYRTKYK ATQNGQLGIA FDVMWFEPMS NTTIDIEAAK 

       310        320        330        340        350        360 
RAQEFQLGWF ADPFFFGDYP ATMRARVGER LPRFTADEAA VVKGALDFVG INHYTTYYTR 

       370        380        390        400        410        420 
HNNTNIIGTL LNNTLADTGT VSLPFKNGKP IGDRANSIWL YIVPRGMRSL MNYVKERYNS 

       430        440        450        460        470        480 
PPVYITENGM DDSNNPFISI KDALKDSKRI KYHNDYLTNL AASIKEDGCD VRGYFAWSLL 

       490        500        510        520 
DNWEWAAGYS SRFGLYFVDY KDNLKRYPKN SVQWFKALLK T 

« Hide

References

« Hide 'large scale' references
[1]"Structural and enzymatic characterization of Os3BGlu6, a rice beta-glucosidase hydrolyzing hydrophobic glycosides and (1->3)- and (1->2)-linked disaccharides."
Seshadri S., Akiyama T., Opassiri R., Kuaprasert B., Cairns J.K.
Plant Physiol. 151:47-58(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 38-521 IN COMPLEX WITH SUBSTRATE ANALOG, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISULFIDE BONDS.
Strain: cv. Yukihikari.
[2]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[5]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[6]"Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY129294 mRNA. Translation: AAN01354.1.
DP000009 Genomic DNA. Translation: ABF94615.1.
AP008209 Genomic DNA. Translation: BAF11272.1.
AK119546 mRNA. Translation: BAG99682.1.
RefSeqNP_001049358.1. NM_001055893.1.
UniGeneOs.15799.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3GNOX-ray1.83A38-521[»]
3GNPX-ray1.80A38-521[»]
3GNRX-ray1.81A38-521[»]
3WBAX-ray1.90A38-521[»]
3WBEX-ray1.97A38-521[»]
ProteinModelPortalQ8L7J2.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEQ8L7J2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS03T0212800-01; OS03T0212800-01; OS03G0212800.
GeneID4332041.
KEGGosa:4332041.

Organism-specific databases

GrameneQ8L7J2.

Phylogenomic databases

eggNOGCOG2723.
KOK01188.
OMAHQLEGAY.
ProtClustDBCLSN2682658.

Enzyme and pathway databases

SABIO-RKQ8L7J2.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8L7J2.

Entry information

Entry nameBGL06_ORYSJ
AccessionPrimary (citable) accession number: Q8L7J2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries