ID   GUN19_ARATH             Reviewed;         626 AA.
AC   Q8L7I0; O82513;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 46.
DE   RecName: Full=Endoglucanase 19;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 19;
DE   Flags: Precursor;
GN   OrderedLocusNames=At4g11050; ORFNames=F2P3.1, T22B4.30;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=20083488; PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G.,
RA   Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N.,
RA   Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M.,
RA   Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M.,
RA   Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T.,
RA   Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I.,
RA   Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P.,
RA   Langham S.-A., McCullagh B., Bilham L., Robben J.,
RA   van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F.,
RA   Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E.,
RA   Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P.,
RA   Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H.,
RA   De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R.,
RA   van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S.,
RA   Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R.,
RA   Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S.,
RA   Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H.,
RA   Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A.,
RA   Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R.,
RA   Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S.,
RA   Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E.,
RA   Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A.,
RA   Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T.,
RA   Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C.,
RA   Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S.,
RA   Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K.,
RA   Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L.,
RA   Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J.,
RA   Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J.,
RA   Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D.,
RA   Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D.,
RA   Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C.,
RA   Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C.,
RA   Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R.,
RA   Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S.,
RA   Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A.,
RA   Chen E., Marra M.A., Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA   Libertini E., Li Y., McQueen-Mason S.J.;
RT   "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene
RT   family.";
RL   J. Mol. Evol. 58:506-515(2004).
CC   -!- CATALYTIC ACTIVITY: Endohydrolysis of (1->4)-beta-D-glucosidic
CC       linkages in cellulose, lichenin and cereal beta-D-glucans.
CC   -!- SUBCELLULAR LOCATION: Secreted (By similarity).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E)
CC       family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC35539.1; Type=Erroneous gene model prediction;
CC       Sequence=CAB43040.1; Type=Erroneous gene model prediction;
CC       Sequence=CAB81206.1; Type=Erroneous gene model prediction;
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DR   EMBL; AF080120; AAC35539.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL049876; CAB43040.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL161518; CAB81206.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AY133685; AAM91619.1; -; mRNA.
DR   IPI; IPI00523137; -.
DR   PIR; T01929; T01929.
DR   RefSeq; NP_192843.2; -.
DR   UniGene; At.33589; -.
DR   HSSP; Q9EYQ2; 1IA6.
DR   CAZy; CBM49; Carbohydrate-Binding Module Family 49.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   GeneID; 826706; -.
DR   GenomeReviews; CT486007_GR; AT4G11050.
DR   KEGG; ath:AT4G11050; -.
DR   NMPDR; fig|3702.1.peg.18770; -.
DR   TAIR; At4g11050; -.
DR   OMA; Q8L7I0; YSANMAM.
DR   BRENDA; 3.2.1.4; 302.
DR   GermOnline; AT4G11050; Arabidopsis thaliana.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0008810; F:cellulase activity; IEA:EC.
DR   GO; GO:0007047; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR012341; 6hp_glycosidase.
DR   InterPro; IPR019028; CBM_49.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR018221; Glyco_hydro_9_AS.
DR   Gene3D; G3DSA:1.50.10.10; CelA/Cel48F_cat; 1.
DR   PANTHER; PTHR22298:SF3; Glyco_hydro_9; 1.
DR   Pfam; PF09478; CBM49; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   PROSITE; PS00592; GLYCOSYL_HYDROL_F9_1; 1.
DR   PROSITE; PS00698; GLYCOSYL_HYDROL_F9_2; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Complete proteome; Glycoprotein; Glycosidase;
KW   Hydrolase; Polysaccharide degradation; Secreted; Signal.
FT   SIGNAL        1     23       Potential.
FT   CHAIN        24    626       Endoglucanase 19.
FT                                /FTId=PRO_0000249271.
FT   ACT_SITE    412    412       By similarity.
FT   ACT_SITE    464    464       By similarity.
FT   ACT_SITE    473    473       By similarity.
FT   CARBOHYD    560    560       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    622    622       N-linked (GlcNAc...) (Potential).
SQ   SEQUENCE   626 AA;  69345 MW;  6F7457C97EEF655F CRC64;
     MGSRTTISIL VVLLLGLVQL AISGHDYKQA LSKSILFFEA QRSGHLPPNQ RVSWRSHSGL
     YDGKSSGVDL VGGYYDAGDN VKFGLPMAFT VTTMCWSIIE YGGQLESNGE LGHAIDAVKW
     GTDYFIKAHP EPNVLYGEVG DGKSDHYCWQ RPEEMTTDRR AYKIDRNNPG SDLAGETAAA
     MAAASIVFRR SDPSYSAELL RHAHQLFEFA DKYRGKYDSS ITVAQKYYRS VSGYNDELLW
     AAAWLYQATN DKYYLDYLGK NGDSMGGTGW SMTEFGWDVK YAGVQTLVAK VLMQGKGGEH
     TAVFERYQQK AEQFMCSLLG KSTKNIKKTP GGLIFRQSWN NMQFVTSASF LATVYSDYLS
     YSKRDLLCSQ GNISPSQLLE FSKSQVDYIL GDNPRATSYM VGYGENYPRQ VHHRGSSIVS
     FNVDQKFVTC RGGYATWFSR KGSDPNVLTG ALVGGPDAYD NFADQRDNYE QTEPATYNNA
     PLLGVLARLI SGSTGFDQLL PGVSPTPSPV IIKPAPVPQR KPTKPPAASS PSPITISQKM
     TNSWKNEGKV YYRYSTILTN RSTKTLKILK ISITKLYGPI WGVTKTGNSF SFPSWMQSLP
     SGKSMEFVYI HSASPADVLV SNYSLE
//