Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8L7G0 (ARFA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Auxin response factor 1
Gene names
Name:ARF1
Ordered Locus Names:At1g59750
ORF Names:F23H11.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length665 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Promotes flowering, stamen development, floral organ abscission and fruit dehiscence. Acts as repressor of IAA2, IAA3 and IAA7. Ref.7 Ref.8

Subunit structure

Homodimers and heterodimers. Interacts with the auxin-responsive proteins IAA12, IAA13, IAA17 and with ARF2. Ref.5

Subcellular location

Nucleus.

Tissue specificity

Expressed in the whole plant. Ref.5

Developmental stage

Expressed in the sepals and carpels of young flower buds. At stage 10 of flower development, expression in the carpels becomes restricted to the style. Also expressed in anthers and filaments. At stage 13, expressed in the region at the top of the pedicel, including the abscission zone. Ref.8

Domain

Interactions between auxin response factors (ARFs) and Aux/IAA proteins occur through their C-terminal dimerization domains III and IV.

Sequence similarities

Belongs to the ARF family.

Contains 1 Aux/IAA-ARF domain.

Contains 1 TF-B3 DNA-binding domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

F3A4.140Q9SN122EBI-2324259,EBI-2324225

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8L7G0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8L7G0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     390-392: Missing.
Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 665665Auxin response factor 1
PRO_0000111505

Regions

Domain543 – 62583Aux/IAA-ARF
DNA binding124 – 226103TF-B3
Compositional bias344 – 41269Pro-rich

Natural variations

Alternative sequence390 – 3923Missing in isoform 2.
VSP_010077

Experimental info

Sequence conflict1361H → Q in AAM91657. Ref.4

Secondary structure

............................................................. 665
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 13, 2004. Version 2.
Checksum: 79DD3180C2091401

FASTA66573,668
        10         20         30         40         50         60 
MAASNHSSGK PGGVLSDALC RELWHACAGP LVTLPREGER VYYFPEGHME QLEASMHQGL 

        70         80         90        100        110        120 
EQQMPSFNLP SKILCKVINI QRRAEPETDE VYAQITLLPE LDQSEPTSPD APVQEPEKCT 

       130        140        150        160        170        180 
VHSFCKTLTA SDTSTHGGFS VLRRHADDCL PPLDMSQQPP WQELVATDLH NSEWHFRHIF 

       190        200        210        220        230        240 
RGQPRRHLLT TGWSVFVSSK KLVAGDAFIF LRGENEELRV GVRRHMRQQT NIPSSVISSH 

       250        260        270        280        290        300 
SMHIGVLATA AHAITTGTIF SVFYKPRTSR SEFIVSVNRY LEAKTQKLSV GMRFKMRFEG 

       310        320        330        340        350        360 
EEAPEKRFSG TIVGVQENKS SVWHDSEWRS LKVQWDEPSS VFRPERVSPW ELEPLVANST 

       370        380        390        400        410        420 
PSSQPQPPQR NKRPRPPGLP SPATGPSGPV TPDGVWKSPA DTPSSVPLFS PPAKAATFGH 

       430        440        450        460        470        480 
GGNKSFGVSI GSAFWPTNAD SAAESFASAF NNESTEKKQT NGNVCRLFGF ELVENVNVDE 

       490        500        510        520        530        540 
CFSAASVSGA VAVDQPVPSN EFDSGQQSEP LNINQSDIPS GSGDPEKSSL RSPQESQSRQ 

       550        560        570        580        590        600 
IRSCTKVHMQ GSAVGRAIDL TRSECYEDLF KKLEEMFDIK GELLESTKKW QVVYTDDEDD 

       610        620        630        640        650        660 
MMMVGDDPWN EFCGMVRKIF IYTPEEVKKL SPKNKLAVNA RMQLKADAEE NGNTEGRSSS 


MAGSR 

« Hide

Isoform 2 [UniParc].

Checksum: 598A0403F161FA54
Show »

FASTA66273,370

References

« Hide 'large scale' references
[1]"ARF1, a transcription factor that binds to auxin response elements."
Ulmasov T., Hagen G., Guilfoyle T.J.
Science 276:1865-1868(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: cv. Columbia.
[5]"Dimerization and DNA binding of auxin response factors."
Ulmasov T., Hagen G., Guilfoyle T.J.
Plant J. 19:309-319(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: DIMERIZATION, TISSUE SPECIFICITY.
[6]"Activation and repression of transcription by auxin-response factors."
Ulmasov T., Hagen G., Guilfoyle T.J.
Proc. Natl. Acad. Sci. U.S.A. 96:5844-5849(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TRANSCRIPTIONAL REPRESSOR.
[7]"Auxin-responsive gene expression: genes, promoters and regulatory factors."
Hagen G., Guilfoyle T.J.
Plant Mol. Biol. 49:373-385(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
[8]"AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana."
Ellis C.M., Nagpal P., Young J.C., Hagen G., Guilfoyle T.J., Reed J.W.
Development 132:4563-4574(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[9]"The plant B3 superfamily."
Swaminathan K., Peterson K., Jack T.
Trends Plant Sci. 13:647-655(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U83245 mRNA. Translation: AAC49751.1.
AC007258 Genomic DNA. Translation: AAD39318.1.
CP002684 Genomic DNA. Translation: AEE33612.1.
CP002684 Genomic DNA. Translation: AEE33613.1.
CP002684 Genomic DNA. Translation: AEE33614.1.
AY133723 mRNA. Translation: AAM91657.1.
BT002748 mRNA. Translation: AAO22577.1.
PIRD96621.
RefSeqNP_001031208.1. NM_001036131.2. [Q8L7G0-1]
NP_176184.1. NM_104668.3. [Q8L7G0-1]
NP_849830.1. NM_179499.2. [Q8L7G0-2]
UniGeneAt.74832.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4LDVX-ray1.45A1-355[»]
4LDWX-ray2.67A/B1-355[»]
4LDXX-ray2.90A/B1-355[»]
4LDYX-ray2.30A/B1-355[»]
ProteinModelPortalQ8L7G0.
SMRQ8L7G0. Positions 15-355, 545-627.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid27493. 10 interactions.
IntActQ8L7G0. 16 interactions.
MINTMINT-7712748.
STRING3702.AT1G59750.1-P.

Proteomic databases

PRIDEQ8L7G0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G59750.1; AT1G59750.1; AT1G59750. [Q8L7G0-1]
AT1G59750.3; AT1G59750.3; AT1G59750. [Q8L7G0-1]
GeneID842268.
KEGGath:AT1G59750.

Organism-specific databases

GeneFarm3363. 345.
TAIRAT1G59750.

Phylogenomic databases

eggNOGNOG257828.
HOGENOMHOG000241141.
InParanoidQ8L7G0.
KOK14486.
OMAQYLPMFN.
PhylomeDBQ8L7G0.

Gene expression databases

GenevestigatorQ8L7G0.

Family and domain databases

Gene3D2.40.330.10. 1 hit.
InterProIPR003311. AUX_IAA.
IPR011525. AuxIAA_ARF_dimer.
IPR010525. Auxin_resp.
IPR003340. B3_DNA-bd.
IPR015300. DNA-bd_pseudobarrel.
[Graphical view]
PfamPF02309. AUX_IAA. 1 hit.
PF06507. Auxin_resp. 1 hit.
PF02362. B3. 1 hit.
[Graphical view]
SMARTSM01019. B3. 1 hit.
[Graphical view]
SUPFAMSSF101936. SSF101936. 1 hit.
PROSITEPS50863. B3. 1 hit.
PS50962. IAA_ARF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameARFA_ARATH
AccessionPrimary (citable) accession number: Q8L7G0
Secondary accession number(s): O23664
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: June 11, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names