Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Auxin response factor 1

Gene

ARF1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Promotes flowering, stamen development, floral organ abscission and fruit dehiscence. Acts as repressor of IAA2, IAA3 and IAA7.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi124 – 226TF-B3PROSITE-ProRule annotationAdd BLAST103

GO - Molecular functioni

  • DNA binding Source: TAIR
  • identical protein binding Source: IntAct
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • leaf senescence Source: TAIR
  • negative regulation of transcription, DNA-templated Source: TAIR
  • response to auxin Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Auxin signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Auxin response factor 1
Gene namesi
Name:ARF1
Ordered Locus Names:At1g59750
ORF Names:F23H11.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G59750.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001115051 – 665Auxin response factor 1Add BLAST665

Proteomic databases

PaxDbiQ8L7G0.

PTM databases

iPTMnetiQ8L7G0.

Expressioni

Tissue specificityi

Expressed in the whole plant.1 Publication

Developmental stagei

Expressed in the sepals and carpels of young flower buds. At stage 10 of flower development, expression in the carpels becomes restricted to the style. Also expressed in anthers and filaments. At stage 13, expressed in the region at the top of the pedicel, including the abscission zone.1 Publication

Gene expression databases

ExpressionAtlasiQ8L7G0. baseline and differential.
GenevisibleiQ8L7G0. AT.

Interactioni

Subunit structurei

Homodimers and heterodimers. Interacts with the auxin-responsive proteins IAA12, IAA13, IAA17 and with ARF2.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-2324259,EBI-2324259
F3A4.140Q9SN122EBI-2324259,EBI-2324225

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi27493. 15 interactors.
IntActiQ8L7G0. 16 interactors.
MINTiMINT-7712748.
STRINGi3702.AT1G59750.1.

Structurei

Secondary structure

1665
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 28Combined sources12
Beta strandi40 – 43Combined sources4
Helixi45 – 52Combined sources8
Beta strandi54 – 56Combined sources3
Beta strandi71 – 84Combined sources14
Beta strandi86 – 88Combined sources3
Beta strandi91 – 99Combined sources9
Beta strandi121 – 127Combined sources7
Helixi130 – 133Combined sources4
Beta strandi134 – 137Combined sources4
Beta strandi139 – 141Combined sources3
Helixi143 – 149Combined sources7
Beta strandi157 – 159Combined sources3
Beta strandi161 – 167Combined sources7
Beta strandi173 – 181Combined sources9
Turni182 – 185Combined sources4
Beta strandi186 – 189Combined sources4
Helixi193 – 199Combined sources7
Beta strandi207 – 213Combined sources7
Beta strandi218 – 221Combined sources4
Helixi239 – 256Combined sources18
Beta strandi260 – 264Combined sources5
Turni266 – 268Combined sources3
Beta strandi274 – 276Combined sources3
Helixi277 – 285Combined sources9
Beta strandi293 – 297Combined sources5
Beta strandi301 – 304Combined sources4
Beta strandi307 – 316Combined sources10
Beta strandi321 – 323Combined sources3
Beta strandi331 – 337Combined sources7
Beta strandi345 – 347Combined sources3
Helixi349 – 351Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LDVX-ray1.45A1-355[»]
4LDWX-ray2.67A/B1-355[»]
4LDXX-ray2.90A/B1-355[»]
4LDYX-ray2.30A/B1-355[»]
ProteinModelPortaliQ8L7G0.
SMRiQ8L7G0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini542 – 635PB1PROSITE-ProRule annotationAdd BLAST94

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi344 – 412Pro-richAdd BLAST69

Domaini

Interactions between auxin response factors (ARFs) and Aux/IAA proteins occur through their C-terminal dimerization domains III and IV.

Sequence similaritiesi

Belongs to the ARF family.Curated
Contains 1 PB1 domain.PROSITE-ProRule annotation
Contains 1 TF-B3 DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IHVY. Eukaryota.
ENOG410Y8ZK. LUCA.
HOGENOMiHOG000241141.
InParanoidiQ8L7G0.
KOiK14486.
OMAiNSEWHFR.
OrthoDBiEOG09360207.
PhylomeDBiQ8L7G0.

Family and domain databases

CDDicd10017. B3_DNA. 1 hit.
Gene3Di2.40.330.10. 1 hit.
InterProiIPR033389. AUX/IAA_dom.
IPR010525. Auxin_resp.
IPR003340. B3_DNA-bd.
IPR015300. DNA-bd_pseudobarrel.
IPR000270. PB1_dom.
[Graphical view]
PfamiPF02309. AUX_IAA. 1 hit.
PF06507. Auxin_resp. 1 hit.
PF02362. B3. 1 hit.
[Graphical view]
SMARTiSM01019. B3. 1 hit.
[Graphical view]
SUPFAMiSSF101936. SSF101936. 1 hit.
PROSITEiPS50863. B3. 1 hit.
PS51745. PB1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8L7G0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASNHSSGK PGGVLSDALC RELWHACAGP LVTLPREGER VYYFPEGHME
60 70 80 90 100
QLEASMHQGL EQQMPSFNLP SKILCKVINI QRRAEPETDE VYAQITLLPE
110 120 130 140 150
LDQSEPTSPD APVQEPEKCT VHSFCKTLTA SDTSTHGGFS VLRRHADDCL
160 170 180 190 200
PPLDMSQQPP WQELVATDLH NSEWHFRHIF RGQPRRHLLT TGWSVFVSSK
210 220 230 240 250
KLVAGDAFIF LRGENEELRV GVRRHMRQQT NIPSSVISSH SMHIGVLATA
260 270 280 290 300
AHAITTGTIF SVFYKPRTSR SEFIVSVNRY LEAKTQKLSV GMRFKMRFEG
310 320 330 340 350
EEAPEKRFSG TIVGVQENKS SVWHDSEWRS LKVQWDEPSS VFRPERVSPW
360 370 380 390 400
ELEPLVANST PSSQPQPPQR NKRPRPPGLP SPATGPSGPV TPDGVWKSPA
410 420 430 440 450
DTPSSVPLFS PPAKAATFGH GGNKSFGVSI GSAFWPTNAD SAAESFASAF
460 470 480 490 500
NNESTEKKQT NGNVCRLFGF ELVENVNVDE CFSAASVSGA VAVDQPVPSN
510 520 530 540 550
EFDSGQQSEP LNINQSDIPS GSGDPEKSSL RSPQESQSRQ IRSCTKVHMQ
560 570 580 590 600
GSAVGRAIDL TRSECYEDLF KKLEEMFDIK GELLESTKKW QVVYTDDEDD
610 620 630 640 650
MMMVGDDPWN EFCGMVRKIF IYTPEEVKKL SPKNKLAVNA RMQLKADAEE
660
NGNTEGRSSS MAGSR
Length:665
Mass (Da):73,668
Last modified:April 13, 2004 - v2
Checksum:i79DD3180C2091401
GO
Isoform 2 (identifier: Q8L7G0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     390-392: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:662
Mass (Da):73,370
Checksum:i598A0403F161FA54
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136H → Q in AAM91657 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010077390 – 392Missing in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83245 mRNA. Translation: AAC49751.1.
AC007258 Genomic DNA. Translation: AAD39318.1.
CP002684 Genomic DNA. Translation: AEE33612.1.
CP002684 Genomic DNA. Translation: AEE33613.1.
CP002684 Genomic DNA. Translation: AEE33614.1.
AY133723 mRNA. Translation: AAM91657.1.
BT002748 mRNA. Translation: AAO22577.1.
PIRiD96621.
RefSeqiNP_001031208.1. NM_001036131.3. [Q8L7G0-1]
NP_176184.1. NM_104668.4. [Q8L7G0-1]
NP_849830.1. NM_179499.2. [Q8L7G0-2]
UniGeneiAt.74832.

Genome annotation databases

EnsemblPlantsiAT1G59750.1; AT1G59750.1; AT1G59750. [Q8L7G0-1]
AT1G59750.3; AT1G59750.3; AT1G59750. [Q8L7G0-1]
GeneIDi842268.
GrameneiAT1G59750.1; AT1G59750.1; AT1G59750.
AT1G59750.3; AT1G59750.3; AT1G59750.
KEGGiath:AT1G59750.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83245 mRNA. Translation: AAC49751.1.
AC007258 Genomic DNA. Translation: AAD39318.1.
CP002684 Genomic DNA. Translation: AEE33612.1.
CP002684 Genomic DNA. Translation: AEE33613.1.
CP002684 Genomic DNA. Translation: AEE33614.1.
AY133723 mRNA. Translation: AAM91657.1.
BT002748 mRNA. Translation: AAO22577.1.
PIRiD96621.
RefSeqiNP_001031208.1. NM_001036131.3. [Q8L7G0-1]
NP_176184.1. NM_104668.4. [Q8L7G0-1]
NP_849830.1. NM_179499.2. [Q8L7G0-2]
UniGeneiAt.74832.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LDVX-ray1.45A1-355[»]
4LDWX-ray2.67A/B1-355[»]
4LDXX-ray2.90A/B1-355[»]
4LDYX-ray2.30A/B1-355[»]
ProteinModelPortaliQ8L7G0.
SMRiQ8L7G0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27493. 15 interactors.
IntActiQ8L7G0. 16 interactors.
MINTiMINT-7712748.
STRINGi3702.AT1G59750.1.

PTM databases

iPTMnetiQ8L7G0.

Proteomic databases

PaxDbiQ8L7G0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G59750.1; AT1G59750.1; AT1G59750. [Q8L7G0-1]
AT1G59750.3; AT1G59750.3; AT1G59750. [Q8L7G0-1]
GeneIDi842268.
GrameneiAT1G59750.1; AT1G59750.1; AT1G59750.
AT1G59750.3; AT1G59750.3; AT1G59750.
KEGGiath:AT1G59750.

Organism-specific databases

TAIRiAT1G59750.

Phylogenomic databases

eggNOGiENOG410IHVY. Eukaryota.
ENOG410Y8ZK. LUCA.
HOGENOMiHOG000241141.
InParanoidiQ8L7G0.
KOiK14486.
OMAiNSEWHFR.
OrthoDBiEOG09360207.
PhylomeDBiQ8L7G0.

Miscellaneous databases

PROiQ8L7G0.

Gene expression databases

ExpressionAtlasiQ8L7G0. baseline and differential.
GenevisibleiQ8L7G0. AT.

Family and domain databases

CDDicd10017. B3_DNA. 1 hit.
Gene3Di2.40.330.10. 1 hit.
InterProiIPR033389. AUX/IAA_dom.
IPR010525. Auxin_resp.
IPR003340. B3_DNA-bd.
IPR015300. DNA-bd_pseudobarrel.
IPR000270. PB1_dom.
[Graphical view]
PfamiPF02309. AUX_IAA. 1 hit.
PF06507. Auxin_resp. 1 hit.
PF02362. B3. 1 hit.
[Graphical view]
SMARTiSM01019. B3. 1 hit.
[Graphical view]
SUPFAMiSSF101936. SSF101936. 1 hit.
PROSITEiPS50863. B3. 1 hit.
PS51745. PB1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARFA_ARATH
AccessioniPrimary (citable) accession number: Q8L7G0
Secondary accession number(s): O23664
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: November 30, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.