Q8L7F9 (B3GTF_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,3-galactosyltransferase 15
EC=2.4.1.-

Alternative name(s):
Galactosyltransferase 1

Gene names

Name:	B3GALT15
Synonyms:	GALT1
Ordered Locus Names:	At1g26810
ORF Names:	T24P13.20

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Involved in the biosynthesis of N-glycans containing Lewis a structures (with the combination of FUT13). Ref.1
Cofactor	Manganese. Ref.1
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Ref.1.
Tissue specificity	Expressed in stems and siliques. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 31 family. Contains 1 galectin domain.
Sequence caution	The sequence AAF87039.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Lewis a epitope biosynthetic process Inferred from mutant phenotype Ref.1. Source: TAIR protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UDP-galactose:N-glycan beta-1,3-galactosyltransferase activity Inferred from direct assay Ref.1. Source: TAIR sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 643	643	Beta-1,3-galactosyltransferase 15		PRO_0000359425
Regions
	Transmembrane	7 – 23	17	Helical; Signal-anchor for type II membrane protein; Potential
	Domain	171 – 364	194	Galectin
Amino acid modifications
	Glycosylation	45	1	N-linked (GlcNAc...) Potential
	Glycosylation	87	1	N-linked (GlcNAc...) Potential
	Glycosylation	144	1	N-linked (GlcNAc...) Potential
	Glycosylation	162	1	N-linked (GlcNAc...) Potential
	Glycosylation	277	1	N-linked (GlcNAc...) Potential
	Glycosylation	287	1	N-linked (GlcNAc...) Potential
	Glycosylation	508	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8L7F9 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6951E000706ADE81	FASTA	643	72,323
	10 20 30 40 50 60 MKRFYGGLLV VSMCMFLTVY RYVDLNTPVE KPYITAAASV VVTPNTTLPM EWLRITLPDF 70 80 90 100 110 120 MKEARNTQEA ISGDDIAVVS GLFVEQNVSK EEREPLLTWN RLESLVDNAQ SLVNGVDAIK 130 140 150 160 170 180 EAGIVWESLV SAVEAKKLVD VNENQTRKGK EELCPQFLSK MNATEADGSS LKLQIPCGLT 190 200 210 220 230 240 QGSSITVIGI PDGLVGSFRI DLTGQPLPGE PDPPIIVHYN VRLLGDKSTE DPVIVQNSWT 250 260 270 280 290 300 ASQDWGAEER CPKFDPDMNK KVDDLDECNK MVGGEINRTS STSLQSNTSR GVPVAREASK 310 320 330 340 350 360 HEKYFPFKQG FLSVATLRVG TEGMQMTVDG KHITSFAFRD TLEPWLVSEI RITGDFRLIS 370 380 390 400 410 420 ILASGLPTSE ESEHVVDLEA LKSPTLSPLR PLDLVIGVFS TANNFKRRMA VRRTWMQYDD 430 440 450 460 470 480 VRSGRVAVRF FVGLHKSPLV NLELWNEART YGDVQLMPFV DYYSLISWKT LAICIFGTEV 490 500 510 520 530 540 DSAKFIMKTD DDAFVRVDEV LLSLSMTNNT RGLIYGLINS DSQPIRNPDS KWYISYEEWP 550 560 570 580 590 600 EEKYPPWAHG PGYIVSRDIA ESVGKLFKEG NLKMFKLEDV AMGIWIAELT KHGLEPHYEN 610 620 630 640 DGRIISDGCK DGYVVAHYQS PAEMTCLWRK YQETKRSLCC REW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A unique beta-1,3-galactosyltransferase is indispensable for the biosynthesis of N-glycans containing Lewis a structures in Arabidopsis thaliana." Strasser R., Bondili J.S., Vavra U., Schoberer J., Svoboda B., Gloessl J., Leonard R., Stadlmann J., Altmann F., Steinkellner H., Mach L. Plant Cell 19:2278-2292(2007) [PubMed: 17630273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, COFACTOR, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: cv. Columbia. Tissue: Silique.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases." Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J., Bacic A. Plant Mol. Biol. 68:43-59(2008) [PubMed: 18548197] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF428439 mRNA. Translation: ABR58858.1. AC006535 Genomic DNA. Translation: AAF87039.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE30744.1. AY133724 mRNA. Translation: AAM91658.1.
IPI	IPI00519343.
PIR	F86394.
RefSeq	NP_174003.1. NM_102445.3.
UniGene	At.41183.
3D structure databases
ProteinModelPortal	Q8L7F9.
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Proteomic databases
PRIDE	Q8L7F9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G26810.1; AT1G26810.1; AT1G26810.
GeneID	839224.
GenomeReviews	Gene locus AT1G26810 in contig CT485782_GR.
KEGG	ath:AT1G26810.
NMPDR	fig|3702.1.peg.3003.
Organism-specific databases
TAIR	At1g26810.
Phylogenomic databases
eggNOG	KOG2287.
GeneTree	EPGT00070000028922.
HOGENOM	HBG318248.
InParanoid	Q8L7F9.
OMA	YDDVRSG.
PhylomeDB	Q8L7F9.
ProtClustDB	PLN03133.
Gene expression databases
Genevestigator	Q8L7F9.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR013320. ConA-like_subgrp. IPR001079. Galectin_CRD. IPR002659. Glyco_trans_31. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 2 hits.
KO	K14413.
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF00337. Gal-bind_lectin. 1 hit. PF01762. Galactosyl_T. 1 hit. [Graphical view]
SMART	SM00908. Gal-bind_lectin. 1 hit. SM00276. GLECT. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS51304. GALECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GTF_ARATH

Accession

Primary (citable) accession number: Q8L7F9
Secondary accession number(s): Q9LQX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program