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Protein

Glutathione S-transferase U20

Gene

GSTU20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits glutathione-dependent thiol transferase activities. Can use glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates. Involved in the regulation of far-red light influence on development.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Kineticsi

  1. KM=0.467 mM for glutathione1 Publication
  2. KM=1.794 mM for 1-chloro-2,4- dinitrobenzene1 Publication

    GO - Molecular functioni

    • enzyme binding Source: UniProtKB
    • glutathione transferase activity Source: UniProtKB

    GO - Biological processi

    • regulation of growth Source: UniProtKB-KW
    • regulation of response to red or far red light Source: UniProtKB
    • response to cytokinin Source: TAIR
    • response to toxic substance Source: UniProtKB-KW
    • toxin catabolic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Detoxification, Growth regulation

    Enzyme and pathway databases

    BioCyciARA:AT1G78370-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase U20 (EC:2.5.1.18)
    Short name:
    AtGSTU20
    Alternative name(s):
    FIN219-interacting protein 1
    GST class-tau member 20
    Gene namesi
    Name:GSTU20
    Synonyms:FIP1
    Ordered Locus Names:At1g78370
    ORF Names:F3F9.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548 Componenti: Chromosome 1

    Organism-specific databases

    TAIRiAT1G78370.

    Subcellular locationi

    GO - Cellular componenti

    • apoplast Source: TAIR
    • chloroplast Source: TAIR
    • cytoplasm Source: UniProtKB
    • cytosol Source: UniProtKB-SubCell
    • nucleus Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Hyposensitive hypocotyl phenotype under continuous far red (cFR) light and a delayed flowering phenotype under long-day conditions.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 217216Glutathione S-transferase U20PRO_0000403932Add
    BLAST

    Proteomic databases

    PaxDbiQ8L7C9.
    PRIDEiQ8L7C9.

    Expressioni

    Tissue specificityi

    Mostly associated with vascular tissues, especially near hydathodes.1 Publication

    Developmental stagei

    Light-dependent expression. Developmentally regulated. First observed in cotyledon vascular tissues of young seedlings. Appears at the shoot apex, in the upper part of hypocotyls and in roots in one week old seedlings. Later highly expressed in the basal portion of trichomes, veins, shoot apex, and hypocotyls. Becomes restricted to the margins of leaves and in roots. In drakness and blue light (B) grown plants, localized in cotyledons vascular tissues. In far-red (FR) and red (R) light conditions, mostly confined to regions of vascular tissues near the hydathode of cotyledons. In adult plants, expressed in vascular tissues of flower organs.1 Publication

    Interactioni

    Subunit structurei

    Interacts with JAR1 under continuous far red (cFR) light.1 Publication

    Protein-protein interaction databases

    BioGridi29392. 2 interactions.
    STRINGi3702.AT1G78370.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L7C9.
    SMRiQ8L7C9. Positions 6-214.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 8280GST N-terminalAdd
    BLAST
    Domaini88 – 208121GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni13 – 142Glutathione bindingBy similarity
    Regioni39 – 402Glutathione bindingBy similarity
    Regioni53 – 542Glutathione bindingBy similarity
    Regioni66 – 672Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Tau family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0625.
    HOGENOMiHOG000125749.
    KOiK00799.
    OMAiYETICNF.
    PhylomeDBiQ8L7C9.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8L7C9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MANLPILLDY WPSMFGMRAR VALREKGVEF EYREEDFSNK SPLLLQSNPI
    60 70 80 90 100
    HKKIPVLVHN GKPVCESLNV VQYVDEAWPE KNPFFPSDPY GRAQARFWAD
    110 120 130 140 150
    FVDKKFTDAQ FKVWGKKGEE QEAGKKEFIE AVKILESELG DKPYFGGDSF
    160 170 180 190 200
    GYVDISLITF SSWFQAYEKF GNFSIESESP KLIAWAKRCM EKESVSKSLP
    210
    DSEKIVAYAA EYRKNNL
    Length:217
    Mass (Da):25,006
    Last modified:October 1, 2002 - v1
    Checksum:i5BD5DEB175079DA9
    GO

    Sequence cautioni

    The sequence AAF71798.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti181 – 1811K → N in AAM64587 (Ref. 4) Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC013430 Genomic DNA. Translation: AAF71798.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE36098.1.
    AY136338 mRNA. Translation: AAM97004.1.
    BT000168 mRNA. Translation: AAN15487.1.
    AY087026 mRNA. Translation: AAM64587.1.
    RefSeqiNP_177958.1. NM_106484.3.
    UniGeneiAt.25338.
    At.72716.

    Genome annotation databases

    EnsemblPlantsiAT1G78370.1; AT1G78370.1; AT1G78370.
    GeneIDi844173.
    KEGGiath:AT1G78370.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC013430 Genomic DNA. Translation: AAF71798.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE36098.1.
    AY136338 mRNA. Translation: AAM97004.1.
    BT000168 mRNA. Translation: AAN15487.1.
    AY087026 mRNA. Translation: AAM64587.1.
    RefSeqiNP_177958.1. NM_106484.3.
    UniGeneiAt.25338.
    At.72716.

    3D structure databases

    ProteinModelPortaliQ8L7C9.
    SMRiQ8L7C9. Positions 6-214.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi29392. 2 interactions.
    STRINGi3702.AT1G78370.1.

    Proteomic databases

    PaxDbiQ8L7C9.
    PRIDEiQ8L7C9.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT1G78370.1; AT1G78370.1; AT1G78370.
    GeneIDi844173.
    KEGGiath:AT1G78370.

    Organism-specific databases

    TAIRiAT1G78370.

    Phylogenomic databases

    eggNOGiCOG0625.
    HOGENOMiHOG000125749.
    KOiK00799.
    OMAiYETICNF.
    PhylomeDBiQ8L7C9.

    Enzyme and pathway databases

    BioCyciARA:AT1G78370-MONOMER.

    Miscellaneous databases

    PROiQ8L7C9.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
      Wagner U., Edwards R., Dixon D.P., Mauch F.
      Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Desensitization of GSTF8 induction by a prior chemical treatment is long lasting and operates in a tissue-dependent manner."
      Foley R.C., Sappl P.G., Perl-Treves R., Millar A.H., Singh K.B.
      Plant Physiol. 142:245-253(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Glutathione S-transferase interacting with far-red insensitive 219 is involved in phytochrome A-mediated signaling in Arabidopsis."
      Chen I.-C., Huang I.-C., Liu M.-J., Wang Z.-G., Chung S.-S., Hsieh H.-L.
      Plant Physiol. 143:1189-1202(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH JAR1, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: cv. Columbia.
    8. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiGSTUK_ARATH
    AccessioniPrimary (citable) accession number: Q8L7C9
    Secondary accession number(s): Q8LBS3, Q9M9F4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2011
    Last sequence update: October 1, 2002
    Last modified: June 24, 2015
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.