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Q8L7C9

- GSTUK_ARATH

UniProt

Q8L7C9 - GSTUK_ARATH

Protein

Glutathione S-transferase U20

Gene

GSTU20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Exhibits glutathione-dependent thiol transferase activities. Can use glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates. Involved in the regulation of far-red light influence on development.1 Publication

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Kineticsi

    1. KM=0.467 mM for glutathione1 Publication
    2. KM=1.794 mM for 1-chloro-2,4- dinitrobenzene1 Publication

    GO - Molecular functioni

    1. enzyme binding Source: UniProtKB
    2. glutathione transferase activity Source: UniProtKB

    GO - Biological processi

    1. regulation of growth Source: UniProtKB-KW
    2. regulation of response to red or far red light Source: UniProtKB
    3. response to toxic substance Source: UniProtKB-KW
    4. toxin catabolic process Source: TAIR

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Detoxification, Growth regulation

    Enzyme and pathway databases

    BioCyciARA:AT1G78370-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase U20 (EC:2.5.1.18)
    Short name:
    AtGSTU20
    Alternative name(s):
    FIN219-interacting protein 1
    GST class-tau member 20
    Gene namesi
    Name:GSTU20
    Synonyms:FIP1
    Ordered Locus Names:At1g78370
    ORF Names:F3F9.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G78370.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasmcytosol 1 Publication

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. chloroplast Source: TAIR
    3. cytoplasm Source: UniProtKB
    4. cytosol Source: UniProtKB-SubCell
    5. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Hyposensitive hypocotyl phenotype under continuous far red (cFR) light and a delayed flowering phenotype under long-day conditions.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 217216Glutathione S-transferase U20PRO_0000403932Add
    BLAST

    Proteomic databases

    PaxDbiQ8L7C9.
    PRIDEiQ8L7C9.

    Expressioni

    Tissue specificityi

    Mostly associated with vascular tissues, especially near hydathodes.1 Publication

    Developmental stagei

    Light-dependent expression. Developmentally regulated. First observed in cotyledon vascular tissues of young seedlings. Appears at the shoot apex, in the upper part of hypocotyls and in roots in one week old seedlings. Later highly expressed in the basal portion of trichomes, veins, shoot apex, and hypocotyls. Becomes restricted to the margins of leaves and in roots. In drakness and blue light (B) grown plants, localized in cotyledons vascular tissues. In far-red (FR) and red (R) light conditions, mostly confined to regions of vascular tissues near the hydathode of cotyledons. In adult plants, expressed in vascular tissues of flower organs.1 Publication

    Gene expression databases

    GenevestigatoriQ8L7C9.

    Interactioni

    Subunit structurei

    Interacts with JAR1 under continuous far red (cFR) light.1 Publication

    Protein-protein interaction databases

    BioGridi29392. 2 interactions.
    STRINGi3702.AT1G78370.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L7C9.
    SMRiQ8L7C9. Positions 6-214.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 8280GST N-terminalAdd
    BLAST
    Domaini88 – 208121GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni13 – 142Glutathione bindingBy similarity
    Regioni39 – 402Glutathione bindingBy similarity
    Regioni53 – 542Glutathione bindingBy similarity
    Regioni66 – 672Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Tau family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0625.
    HOGENOMiHOG000125749.
    InParanoidiQ8L7C9.
    KOiK00799.
    OMAiSYEACAN.
    PhylomeDBiQ8L7C9.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8L7C9-1 [UniParc]FASTAAdd to Basket

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    MANLPILLDY WPSMFGMRAR VALREKGVEF EYREEDFSNK SPLLLQSNPI    50
    HKKIPVLVHN GKPVCESLNV VQYVDEAWPE KNPFFPSDPY GRAQARFWAD 100
    FVDKKFTDAQ FKVWGKKGEE QEAGKKEFIE AVKILESELG DKPYFGGDSF 150
    GYVDISLITF SSWFQAYEKF GNFSIESESP KLIAWAKRCM EKESVSKSLP 200
    DSEKIVAYAA EYRKNNL 217
    Length:217
    Mass (Da):25,006
    Last modified:October 1, 2002 - v1
    Checksum:i5BD5DEB175079DA9
    GO

    Sequence cautioni

    The sequence AAF71798.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti181 – 1811K → N in AAM64587. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC013430 Genomic DNA. Translation: AAF71798.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE36098.1.
    AY136338 mRNA. Translation: AAM97004.1.
    BT000168 mRNA. Translation: AAN15487.1.
    AY087026 mRNA. Translation: AAM64587.1.
    RefSeqiNP_177958.1. NM_106484.3.
    UniGeneiAt.25338.
    At.72716.

    Genome annotation databases

    EnsemblPlantsiAT1G78370.1; AT1G78370.1; AT1G78370.
    GeneIDi844173.
    KEGGiath:AT1G78370.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC013430 Genomic DNA. Translation: AAF71798.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE36098.1 .
    AY136338 mRNA. Translation: AAM97004.1 .
    BT000168 mRNA. Translation: AAN15487.1 .
    AY087026 mRNA. Translation: AAM64587.1 .
    RefSeqi NP_177958.1. NM_106484.3.
    UniGenei At.25338.
    At.72716.

    3D structure databases

    ProteinModelPortali Q8L7C9.
    SMRi Q8L7C9. Positions 6-214.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 29392. 2 interactions.
    STRINGi 3702.AT1G78370.1-P.

    Proteomic databases

    PaxDbi Q8L7C9.
    PRIDEi Q8L7C9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G78370.1 ; AT1G78370.1 ; AT1G78370 .
    GeneIDi 844173.
    KEGGi ath:AT1G78370.

    Organism-specific databases

    TAIRi AT1G78370.

    Phylogenomic databases

    eggNOGi COG0625.
    HOGENOMi HOG000125749.
    InParanoidi Q8L7C9.
    KOi K00799.
    OMAi SYEACAN.
    PhylomeDBi Q8L7C9.

    Enzyme and pathway databases

    BioCyci ARA:AT1G78370-MONOMER.

    Gene expression databases

    Genevestigatori Q8L7C9.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
      Wagner U., Edwards R., Dixon D.P., Mauch F.
      Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Desensitization of GSTF8 induction by a prior chemical treatment is long lasting and operates in a tissue-dependent manner."
      Foley R.C., Sappl P.G., Perl-Treves R., Millar A.H., Singh K.B.
      Plant Physiol. 142:245-253(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Glutathione S-transferase interacting with far-red insensitive 219 is involved in phytochrome A-mediated signaling in Arabidopsis."
      Chen I.-C., Huang I.-C., Liu M.-J., Wang Z.-G., Chung S.-S., Hsieh H.-L.
      Plant Physiol. 143:1189-1202(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH JAR1, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: cv. Columbia.
    8. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiGSTUK_ARATH
    AccessioniPrimary (citable) accession number: Q8L7C9
    Secondary accession number(s): Q8LBS3, Q9M9F4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2011
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3