Q8L7C9 (GSTUK_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase U20
Short name=AtGSTU20
EC=2.5.1.18

Alternative name(s):
FIN219-interacting protein 1
GST class-tau member 20

Gene names

Name:	GSTU20
Synonyms:	FIP1
Ordered Locus Names:	At1g78370
ORF Names:	F3F9.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Exhibits glutathione-dependent thiol transferase activities. Can use glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates. Involved in the regulation of far-red light influence on development. Ref.7
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Interacts with JAR1 under continuous far red (cFR) light. Ref.7
Subcellular location	Nucleus. Cytoplasm › cytosol Ref.7.
Tissue specificity	Mostly associated with vascular tissues, especially near hydathodes. Ref.7
Developmental stage	Light-dependent expression. Developmentally regulated. First observed in cotyledon vascular tissues of young seedlings. Appears at the shoot apex, in the upper part of hypocotyls and in roots in one week old seedlings. Later highly expressed in the basal portion of trichomes, veins, shoot apex, and hypocotyls. Becomes restricted to the margins of leaves and in roots. In drakness and blue light (B) grown plants, localized in cotyledons vascular tissues. In far-red (FR) and red (R) light conditions, mostly confined to regions of vascular tissues near the hydathode of cotyledons. In adult plants, expressed in vascular tissues of flower organs. Ref.7
Disruption phenotype	Hyposensitive hypocotyl phenotype under continuous far red (cFR) light and a delayed flowering phenotype under long-day conditions. Ref.7
Sequence similarities	Belongs to the GST superfamily. Tau family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Biophysicochemical properties	Kinetic parameters: KM=0.467 mM for glutathione Ref.7 KM=1.794 mM for 1-chloro-2,4- dinitrobenzene
Sequence caution	The sequence AAF71798.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Detoxification Growth regulation
Cellular component	Cytoplasm Nucleus
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW regulation of response to red or far red light Inferred from mutant phenotype Ref.7. Source: UniProtKB response to toxin Inferred from electronic annotation. Source: UniProtKB-KW toxin catabolic process Traceable author statement Ref.5. Source: TAIR
Cellular_component	apoplast Inferred from direct assay PubMed 18538804. Source: TAIR chloroplast Inferred from direct assay Ref.8. Source: TAIR cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.7. Source: UniProtKB
Molecular_function	glutathione transferase activity Inferred from mutant phenotype Ref.7. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Glutathione S-transferase U20		PRO_0000403932
Regions
	Domain	3 – 82	80	GST N-terminal
	Domain	88 – 208	121	GST C-terminal
	Region	13 – 14	2	Glutathione binding By similarity
	Region	39 – 40	2	Glutathione binding By similarity
	Region	53 – 54	2	Glutathione binding By similarity
	Region	66 – 67	2	Glutathione binding By similarity
Experimental info
	Sequence conflict	181	1	K → N in AAM64587. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8L7C9 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 5BD5DEB175079DA9	FASTA	217	25,006
	10 20 30 40 50 60 MANLPILLDY WPSMFGMRAR VALREKGVEF EYREEDFSNK SPLLLQSNPI HKKIPVLVHN 70 80 90 100 110 120 GKPVCESLNV VQYVDEAWPE KNPFFPSDPY GRAQARFWAD FVDKKFTDAQ FKVWGKKGEE 130 140 150 160 170 180 QEAGKKEFIE AVKILESELG DKPYFGGDSF GYVDISLITF SSWFQAYEKF GNFSIESESP 190 200 210 KLIAWAKRCM EKESVSKSLP DSEKIVAYAA EYRKNNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Desensitization of GSTF8 induction by a prior chemical treatment is long lasting and operates in a tissue-dependent manner." Foley R.C., Sappl P.G., Perl-Treves R., Millar A.H., Singh K.B. Plant Physiol. 142:245-253(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[7]	"Glutathione S-transferase interacting with far-red insensitive 219 is involved in phytochrome A-mediated signaling in Arabidopsis." Chen I.-C., Huang I.-C., Liu M.-J., Wang Z.-G., Chung S.-S., Hsieh H.-L. Plant Physiol. 143:1189-1202(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH JAR1, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: cv. Columbia.
[8]	"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome." Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J. PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC013430 Genomic DNA. Translation: AAF71798.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE36098.1. AY136338 mRNA. Translation: AAM97004.1. BT000168 mRNA. Translation: AAN15487.1. AY087026 mRNA. Translation: AAM64587.1.
IPI	IPI00540173.
RefSeq	NP_177958.1. NM_106484.3.
UniGene	At.25338. At.72716.
3D structure databases
HSSP	HSSP built from PDB template 1OYJ based on UniProtKB O65032.
ProteinModelPortal	Q8L7C9.
SMR	Q8L7C9. Positions 6-214.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G78370.1-P.
Proteomic databases
PaxDb	Q8L7C9.
PRIDE	Q8L7C9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G78370.1; AT1G78370.1; AT1G78370.
GeneID	844173.
KEGG	ath:AT1G78370.
Organism-specific databases
TAIR	At1g78370.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125749.
InParanoid	Q8L7C9.
KO	K00799.
OMA	RSVARFW.
Gene expression databases
Genevestigator	Q8L7C9.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTUK_ARATH

Accession

Primary (citable) accession number: Q8L7C9
Secondary accession number(s): Q8LBS3, Q9M9F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2011
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program