Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8L7C9

- GSTUK_ARATH

UniProt

Q8L7C9 - GSTUK_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glutathione S-transferase U20

Gene

GSTU20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Exhibits glutathione-dependent thiol transferase activities. Can use glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates. Involved in the regulation of far-red light influence on development.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Kineticsi

  1. KM=0.467 mM for glutathione1 Publication
  2. KM=1.794 mM for 1-chloro-2,4- dinitrobenzene1 Publication

GO - Molecular functioni

  1. enzyme binding Source: UniProtKB
  2. glutathione transferase activity Source: UniProtKB

GO - Biological processi

  1. regulation of growth Source: UniProtKB-KW
  2. regulation of response to red or far red light Source: UniProtKB
  3. response to cytokinin Source: TAIR
  4. response to toxic substance Source: UniProtKB-KW
  5. toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Detoxification, Growth regulation

Enzyme and pathway databases

BioCyciARA:AT1G78370-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase U20 (EC:2.5.1.18)
Short name:
AtGSTU20
Alternative name(s):
FIN219-interacting protein 1
GST class-tau member 20
Gene namesi
Name:GSTU20
Synonyms:FIP1
Ordered Locus Names:At1g78370
ORF Names:F3F9.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G78370.

Subcellular locationi

Nucleus 1 Publication. Cytoplasmcytosol 1 Publication

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. chloroplast Source: TAIR
  3. cytoplasm Source: UniProtKB
  4. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Hyposensitive hypocotyl phenotype under continuous far red (cFR) light and a delayed flowering phenotype under long-day conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 217216Glutathione S-transferase U20PRO_0000403932Add
BLAST

Proteomic databases

PaxDbiQ8L7C9.
PRIDEiQ8L7C9.

Expressioni

Tissue specificityi

Mostly associated with vascular tissues, especially near hydathodes.1 Publication

Developmental stagei

Light-dependent expression. Developmentally regulated. First observed in cotyledon vascular tissues of young seedlings. Appears at the shoot apex, in the upper part of hypocotyls and in roots in one week old seedlings. Later highly expressed in the basal portion of trichomes, veins, shoot apex, and hypocotyls. Becomes restricted to the margins of leaves and in roots. In drakness and blue light (B) grown plants, localized in cotyledons vascular tissues. In far-red (FR) and red (R) light conditions, mostly confined to regions of vascular tissues near the hydathode of cotyledons. In adult plants, expressed in vascular tissues of flower organs.1 Publication

Gene expression databases

GenevestigatoriQ8L7C9.

Interactioni

Subunit structurei

Interacts with JAR1 under continuous far red (cFR) light.1 Publication

Protein-protein interaction databases

BioGridi29392. 2 interactions.
STRINGi3702.AT1G78370.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8L7C9.
SMRiQ8L7C9. Positions 6-214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 8280GST N-terminalAdd
BLAST
Domaini88 – 208121GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 142Glutathione bindingBy similarity
Regioni39 – 402Glutathione bindingBy similarity
Regioni53 – 542Glutathione bindingBy similarity
Regioni66 – 672Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Tau family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0625.
HOGENOMiHOG000125749.
KOiK00799.
OMAiSYEACAN.
PhylomeDBiQ8L7C9.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L7C9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANLPILLDY WPSMFGMRAR VALREKGVEF EYREEDFSNK SPLLLQSNPI
60 70 80 90 100
HKKIPVLVHN GKPVCESLNV VQYVDEAWPE KNPFFPSDPY GRAQARFWAD
110 120 130 140 150
FVDKKFTDAQ FKVWGKKGEE QEAGKKEFIE AVKILESELG DKPYFGGDSF
160 170 180 190 200
GYVDISLITF SSWFQAYEKF GNFSIESESP KLIAWAKRCM EKESVSKSLP
210
DSEKIVAYAA EYRKNNL
Length:217
Mass (Da):25,006
Last modified:October 1, 2002 - v1
Checksum:i5BD5DEB175079DA9
GO

Sequence cautioni

The sequence AAF71798.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811K → N in AAM64587. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC013430 Genomic DNA. Translation: AAF71798.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36098.1.
AY136338 mRNA. Translation: AAM97004.1.
BT000168 mRNA. Translation: AAN15487.1.
AY087026 mRNA. Translation: AAM64587.1.
RefSeqiNP_177958.1. NM_106484.3.
UniGeneiAt.25338.
At.72716.

Genome annotation databases

EnsemblPlantsiAT1G78370.1; AT1G78370.1; AT1G78370.
GeneIDi844173.
KEGGiath:AT1G78370.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC013430 Genomic DNA. Translation: AAF71798.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE36098.1 .
AY136338 mRNA. Translation: AAM97004.1 .
BT000168 mRNA. Translation: AAN15487.1 .
AY087026 mRNA. Translation: AAM64587.1 .
RefSeqi NP_177958.1. NM_106484.3.
UniGenei At.25338.
At.72716.

3D structure databases

ProteinModelPortali Q8L7C9.
SMRi Q8L7C9. Positions 6-214.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 29392. 2 interactions.
STRINGi 3702.AT1G78370.1-P.

Proteomic databases

PaxDbi Q8L7C9.
PRIDEi Q8L7C9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G78370.1 ; AT1G78370.1 ; AT1G78370 .
GeneIDi 844173.
KEGGi ath:AT1G78370.

Organism-specific databases

TAIRi AT1G78370.

Phylogenomic databases

eggNOGi COG0625.
HOGENOMi HOG000125749.
KOi K00799.
OMAi SYEACAN.
PhylomeDBi Q8L7C9.

Enzyme and pathway databases

BioCyci ARA:AT1G78370-MONOMER.

Gene expression databases

Genevestigatori Q8L7C9.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
    Wagner U., Edwards R., Dixon D.P., Mauch F.
    Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Desensitization of GSTF8 induction by a prior chemical treatment is long lasting and operates in a tissue-dependent manner."
    Foley R.C., Sappl P.G., Perl-Treves R., Millar A.H., Singh K.B.
    Plant Physiol. 142:245-253(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Glutathione S-transferase interacting with far-red insensitive 219 is involved in phytochrome A-mediated signaling in Arabidopsis."
    Chen I.-C., Huang I.-C., Liu M.-J., Wang Z.-G., Chung S.-S., Hsieh H.-L.
    Plant Physiol. 143:1189-1202(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH JAR1, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Columbia.
  8. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiGSTUK_ARATH
AccessioniPrimary (citable) accession number: Q8L7C9
Secondary accession number(s): Q8LBS3, Q9M9F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3