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Q8L7B2 (SCP20_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine carboxypeptidase-like 20

EC=3.4.16.-
Gene names
Name:SCPL20
Ordered Locus Names:At4g12910
ORF Names:F25G13.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probable carboxypeptidase By similarity.

Subcellular location

Secreted Potential.

Tissue specificity

Ubiquitous. Ref.4

Sequence similarities

Belongs to the peptidase S10 family.

Sequence caution

The sequence CAB53091.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB53091.1 differs from that shown. Reason: Frameshift at position 479.

The sequence CAB78333.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB78333.1 differs from that shown. Reason: Frameshift at position 479.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionCarboxypeptidase
Hydrolase
Protease
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

vacuole

Inferred from direct assay PubMed 15539469. Source: TAIR

   Molecular_functionserine-type carboxypeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 497468Serine carboxypeptidase-like 20
PRO_0000274636

Regions

Motif495 – 4973Microbody targeting signal Potential

Sites

Active site1861 By similarity
Active site4211 By similarity
Active site4741 By similarity

Amino acid modifications

Glycosylation1111N-linked (GlcNAc...) Potential
Glycosylation1461N-linked (GlcNAc...) Potential
Glycosylation2491N-linked (GlcNAc...) Potential
Glycosylation4051N-linked (GlcNAc...) Potential
Glycosylation4631N-linked (GlcNAc...) Potential
Disulfide bond90 ↔ 386 By similarity
Disulfide bond254 ↔ 266 By similarity
Disulfide bond289 ↔ 353 By similarity

Experimental info

Sequence conflict2211G → V in AAM97031. Ref.3
Sequence conflict2211G → V in AAN15500. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8L7B2 [UniParc].

Last modified February 6, 2007. Version 2.
Checksum: 0258C97B8C6A9D8B

FASTA49755,833
        10         20         30         40         50         60 
MSIITMVWLM KVFVFVTLLS LVFVITESAP ESALITKLPG FEGTFPSKHY SGYVTIDKEH 

        70         80         90        100        110        120 
GKNLWYYFIE SEKNPSKDPV VLWLNGGPGC SSMDGFVYEH GPFNFELPKK NNSLPLLHLN 

       130        140        150        160        170        180 
PYSWSKVSNI IYLDSPVGVG FSYSNNKSDY ITGDIKTAVD SHAFLLKWFQ MFPEFQSNPF 

       190        200        210        220        230        240 
FISGESYAGV YVPTLASEVV IGNKNGVKPA LNFKGYLVGN GVADPKFDGN AFVPFAHGMG 

       250        260        270        280        290        300 
LISDELFENV TKACKGNFYE IEGLECEEQY TKVNDDTNQL NIYNILEPCY HGTSLSAFDI 

       310        320        330        340        350        360 
RSLPSSLLQL GKTEKRLPIR KRMFGRAWPV RAPVHPGIVP SWSQLLADVT VPCIDDRVAT 

       370        380        390        400        410        420 
AWLNDPEIRK AIHTKEESEI GRWELCSGKL SFYHDAGSMI DFHRNLTLSG YRALIYSGDH 

       430        440        450        460        470        480 
DMCVPFTGSE AWTKSLGYKV IDEWRAWISN DQVAGYTQGY ANNLTFLTIK GAGHTVPEYK 

       490 
PREALDFYSR FLEGSKI 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"An expression and bioinformatics analysis of the Arabidopsis serine carboxypeptidase-like gene family."
Fraser C.M., Rider L.W., Chapple C.
Plant Physiol. 138:1136-1148(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, TISSUE SPECIFICITY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL079349 Genomic DNA. Translation: CAB53091.1. Sequence problems.
AL161535 Genomic DNA. Translation: CAB78333.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83202.1.
AY136365 mRNA. Translation: AAM97031.1.
BT000181 mRNA. Translation: AAN15500.1.
PIRA85139.
RefSeqNP_193027.5. NM_117360.7.
UniGeneAt.45003.

3D structure databases

ProteinModelPortalQ8L7B2.
SMRQ8L7B2. Positions 28-496.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT4G12910.1-P.

Protein family/group databases

MEROPSS10.A11.

Proteomic databases

PaxDbQ8L7B2.
PRIDEQ8L7B2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G12910.1; AT4G12910.1; AT4G12910.
GeneID826903.
KEGGath:AT4G12910.

Organism-specific databases

TAIRAT4G12910.

Phylogenomic databases

eggNOGCOG2939.
HOGENOMHOG000198297.
InParanoidQ8L7B2.
KOK16296.
OMADYLVTHD.
PhylomeDBQ8L7B2.

Enzyme and pathway databases

BioCycARA:AT4G12910-MONOMER.

Gene expression databases

GenevestigatorQ8L7B2.

Family and domain databases

Gene3D3.40.50.1820. 2 hits.
InterProIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERPTHR11802. PTHR11802. 1 hit.
PfamPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSPR00724. CRBOXYPTASEC.
SUPFAMSSF53474. SSF53474. 2 hits.
PROSITEPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSCP20_ARATH
AccessionPrimary (citable) accession number: Q8L7B2
Secondary accession number(s): Q9SV78
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: June 11, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names