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Protein

Serine carboxypeptidase-like 20

Gene

SCPL20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Probable carboxypeptidase.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei186By similarity1
Active sitei421By similarity1
Active sitei474By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease

Protein family/group databases

ESTHERiarath-SCP20 Carboxypeptidase_S10
MEROPSiS10.A11

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase-like 20 (EC:3.4.16.-)
Gene namesi
Name:SCPL20
Ordered Locus Names:At4g12910
ORF Names:F25G13.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G12910
TAIRilocus:2123186 AT4G12910

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000027463630 – 497Serine carboxypeptidase-like 20Add BLAST468

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi90 ↔ 386By similarity
Glycosylationi111N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi146N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi249N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi254 ↔ 266By similarity
Disulfide bondi289 ↔ 353By similarity
Glycosylationi405N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi463N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8L7B2
PRIDEiQ8L7B2

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

ExpressionAtlasiQ8L7B2 baseline and differential
GenevisibleiQ8L7B2 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G12910.1

Structurei

3D structure databases

ProteinModelPortaliQ8L7B2
SMRiQ8L7B2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi495 – 497Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1282 Eukaryota
COG2939 LUCA
HOGENOMiHOG000198297
InParanoidiQ8L7B2
KOiK16296
OMAiDPPCTNT
OrthoDBiEOG093608NR
PhylomeDBiQ8L7B2

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR001563 Peptidase_S10
IPR033124 Ser_caboxypep_his_AS
IPR018202 Ser_caboxypep_ser_AS
PANTHERiPTHR11802 PTHR11802, 1 hit
PfamiView protein in Pfam
PF00450 Peptidase_S10, 1 hit
PRINTSiPR00724 CRBOXYPTASEC
SUPFAMiSSF53474 SSF53474, 2 hits
PROSITEiView protein in PROSITE
PS00560 CARBOXYPEPT_SER_HIS, 1 hit
PS00131 CARBOXYPEPT_SER_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L7B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIITMVWLM KVFVFVTLLS LVFVITESAP ESALITKLPG FEGTFPSKHY
60 70 80 90 100
SGYVTIDKEH GKNLWYYFIE SEKNPSKDPV VLWLNGGPGC SSMDGFVYEH
110 120 130 140 150
GPFNFELPKK NNSLPLLHLN PYSWSKVSNI IYLDSPVGVG FSYSNNKSDY
160 170 180 190 200
ITGDIKTAVD SHAFLLKWFQ MFPEFQSNPF FISGESYAGV YVPTLASEVV
210 220 230 240 250
IGNKNGVKPA LNFKGYLVGN GVADPKFDGN AFVPFAHGMG LISDELFENV
260 270 280 290 300
TKACKGNFYE IEGLECEEQY TKVNDDTNQL NIYNILEPCY HGTSLSAFDI
310 320 330 340 350
RSLPSSLLQL GKTEKRLPIR KRMFGRAWPV RAPVHPGIVP SWSQLLADVT
360 370 380 390 400
VPCIDDRVAT AWLNDPEIRK AIHTKEESEI GRWELCSGKL SFYHDAGSMI
410 420 430 440 450
DFHRNLTLSG YRALIYSGDH DMCVPFTGSE AWTKSLGYKV IDEWRAWISN
460 470 480 490
DQVAGYTQGY ANNLTFLTIK GAGHTVPEYK PREALDFYSR FLEGSKI
Length:497
Mass (Da):55,833
Last modified:February 6, 2007 - v2
Checksum:i0258C97B8C6A9D8B
GO

Sequence cautioni

The sequence CAB53091 differs from that shown. Reason: Frameshift at position 479.Curated
The sequence CAB53091 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB78333 differs from that shown. Reason: Frameshift at position 479.Curated
The sequence CAB78333 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti221G → V in AAM97031 (PubMed:14593172).Curated1
Sequence conflicti221G → V in AAN15500 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL079349 Genomic DNA Translation: CAB53091.1 Sequence problems.
AL161535 Genomic DNA Translation: CAB78333.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE83202.1
AY136365 mRNA Translation: AAM97031.1
BT000181 mRNA Translation: AAN15500.1
PIRiA85139
RefSeqiNP_193027.5, NM_117360.8
UniGeneiAt.45003

Genome annotation databases

EnsemblPlantsiAT4G12910.1; AT4G12910.1; AT4G12910
GeneIDi826903
GrameneiAT4G12910.1; AT4G12910.1; AT4G12910
KEGGiath:AT4G12910

Similar proteinsi

Entry informationi

Entry nameiSCP20_ARATH
AccessioniPrimary (citable) accession number: Q8L7B2
Secondary accession number(s): Q9SV78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: May 23, 2018
This is version 93 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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