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Q8L799

- MIOX1_ARATH

UniProt

Q8L799 - MIOX1_ARATH

Protein

Inositol oxygenase 1

Gene

MIOX1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis.2 Publications

    Catalytic activityi

    Myo-inositol + O2 = D-glucuronate + H2O.

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521SubstrateBy similarity
    Metal bindingi122 – 1221Iron 1By similarity
    Metal bindingi147 – 1471Iron 1By similarity
    Metal bindingi148 – 1481Iron 1By similarity
    Metal bindingi148 – 1481Iron 2By similarity
    Binding sitei151 – 1511SubstrateBy similarity
    Metal bindingi220 – 2201Iron 2By similarity
    Metal bindingi246 – 2461Iron 2By similarity
    Metal bindingi279 – 2791Iron 1By similarity

    GO - Molecular functioni

    1. inositol oxygenase activity Source: TAIR
    2. iron ion binding Source: InterPro

    GO - Biological processi

    1. inositol catabolic process Source: InterPro
    2. L-ascorbic acid biosynthetic process Source: UniProtKB-KW
    3. syncytium formation Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Ascorbate biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:GQT-1164-MONOMER.
    MetaCyc:AT1G14520-MONOMER.
    UniPathwayiUPA00111; UER00527.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol oxygenase 1 (EC:1.13.99.1)
    Alternative name(s):
    Myo-inositol oxygenase 1
    Short name:
    AtMIOX1
    Short name:
    MI oxygenase 1
    Gene namesi
    Name:MIOX1
    Ordered Locus Names:At1g14520
    ORF Names:F14L17.30, T5E21.2, T5E21_19
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G14520.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 311311Inositol oxygenase 1PRO_0000079154Add
    BLAST

    Proteomic databases

    PaxDbiQ8L799.
    PRIDEiQ8L799.

    Expressioni

    Tissue specificityi

    Expressed in roots, young leaves, stems, flowers and siliques.1 Publication

    Gene expression databases

    GenevestigatoriQ8L799.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L799.
    SMRiQ8L799. Positions 68-311.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni109 – 1113Substrate bindingBy similarity
    Regioni168 – 1692Substrate bindingBy similarity
    Regioni246 – 2472Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the myo-inositol oxygenase family.Curated

    Phylogenomic databases

    eggNOGiNOG135479.
    HOGENOMiHOG000163182.
    InParanoidiQ8L799.
    KOiK00469.
    OMAiNFYREQH.
    PhylomeDBiQ8L799.

    Family and domain databases

    InterProiIPR007828. Inositol_oxygenase.
    [Graphical view]
    PANTHERiPTHR12588. PTHR12588. 1 hit.
    PfamiPF05153. DUF706. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q8L799-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTILIDRHSD QNDAGDEIVE KNQGNGKEEE TELVLDAGFE APHTNSFGRT    50
    FRDYDAESER RRGVEEFYRV NHIGQTVDFV RKMREEYEKL NRTEMSIWEC 100
    CELLNEFIDE SDPDLDEPQI EHLLQTAEAI RKDYPDEDWL HLTGLIHDLG 150
    KVLLHSSFGE LPQWAVVGDT FPVGCAFDES IVHHKYFKEN PDYDNPSYNS 200
    KYGIYTEGCG LDNVLMSWGH DDYMYLVAKE NQTTLPSAGL FIIRYHSFYA 250
    LHKSEAYKHL MNNEDRENMK WLKVFNKYDL YSKSKVRVNV EEVKPYYLSL 300
    TNKYFPSKLK W 311
    Length:311
    Mass (Da):36,574
    Last modified:October 1, 2002 - v1
    Checksum:i0BCBDE175D505F59
    GO

    Sequence cautioni

    The sequence AAF43953.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence AAF63180.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti111 – 1111S → G in BAC42925. (PubMed:11910074)Curated
    Sequence conflicti111 – 1111S → G in BAD43581. 1 PublicationCurated
    Sequence conflicti111 – 1111S → G in BAD43596. 1 PublicationCurated
    Sequence conflicti111 – 1111S → G in BAD94364. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC010657 Genomic DNA. Translation: AAF63180.1. Sequence problems.
    AC012188 Genomic DNA. Translation: AAF43953.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE29174.1.
    AY136388 mRNA. Translation: AAM97054.1.
    BT000187 mRNA. Translation: AAN15506.1.
    AK118307 mRNA. Translation: BAC42925.1.
    AK175115 mRNA. Translation: BAD42878.1.
    AK175818 mRNA. Translation: BAD43581.1.
    AK175833 mRNA. Translation: BAD43596.1.
    AK176690 mRNA. Translation: BAD44453.1.
    AK221931 mRNA. Translation: BAD94364.1.
    RefSeqiNP_172904.2. NM_101319.3. [Q8L799-1]
    UniGeneiAt.23143.

    Genome annotation databases

    EnsemblPlantsiAT1G14520.1; AT1G14520.1; AT1G14520. [Q8L799-1]
    GeneIDi838014.
    KEGGiath:AT1G14520.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC010657 Genomic DNA. Translation: AAF63180.1 . Sequence problems.
    AC012188 Genomic DNA. Translation: AAF43953.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE29174.1 .
    AY136388 mRNA. Translation: AAM97054.1 .
    BT000187 mRNA. Translation: AAN15506.1 .
    AK118307 mRNA. Translation: BAC42925.1 .
    AK175115 mRNA. Translation: BAD42878.1 .
    AK175818 mRNA. Translation: BAD43581.1 .
    AK175833 mRNA. Translation: BAD43596.1 .
    AK176690 mRNA. Translation: BAD44453.1 .
    AK221931 mRNA. Translation: BAD94364.1 .
    RefSeqi NP_172904.2. NM_101319.3. [Q8L799-1 ]
    UniGenei At.23143.

    3D structure databases

    ProteinModelPortali Q8L799.
    SMRi Q8L799. Positions 68-311.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q8L799.
    PRIDEi Q8L799.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G14520.1 ; AT1G14520.1 ; AT1G14520 . [Q8L799-1 ]
    GeneIDi 838014.
    KEGGi ath:AT1G14520.

    Organism-specific databases

    TAIRi AT1G14520.

    Phylogenomic databases

    eggNOGi NOG135479.
    HOGENOMi HOG000163182.
    InParanoidi Q8L799.
    KOi K00469.
    OMAi NFYREQH.
    PhylomeDBi Q8L799.

    Enzyme and pathway databases

    UniPathwayi UPA00111 ; UER00527 .
    BioCyci ARA:GQT-1164-MONOMER.
    MetaCyc:AT1G14520-MONOMER.

    Gene expression databases

    Genevestigatori Q8L799.

    Family and domain databases

    InterProi IPR007828. Inositol_oxygenase.
    [Graphical view ]
    PANTHERi PTHR12588. PTHR12588. 1 hit.
    Pfami PF05153. DUF706. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides."
      Kanter U., Usadel B., Guerineau F., Li Y., Pauly M., Tenhaken R.
      Planta 221:243-254(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Myo-inositol oxygenase offers a possible entry point into plant ascorbate biosynthesis."
      Lorence A., Chevone B.I., Mendes P., Nessler C.L.
      Plant Physiol. 134:1200-1205(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMIOX1_ARATH
    AccessioniPrimary (citable) accession number: Q8L799
    Secondary accession number(s): Q8GXC4, Q9M9R1, Q9MA30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3