ID   PI5K2_ARATH             Reviewed;         754 AA.
AC   Q8L796; Q9CA20;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 2.
DT   07-JUL-2009, entry version 45.
DE   RecName: Full=Phosphatidylinositol-4-phosphate 5-kinase 2;
DE            Short=AtPIP5K2;
DE            EC=2.7.1.68;
DE   AltName: Full=1-phosphatidylinositol-4-phosphate kinase 2;
DE   AltName: Full=PtdIns(4)P-5-kinase 2;
DE   AltName: Full=Diphosphoinositide kinase 2;
GN   Name=PIP5K2; OrderedLocusNames=At1g77740; ORFNames=T32E8.7;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12226484; DOI=10.1104/pp.004770;
RA   Mueller-Roeber B., Pical C.;
RT   "Inositol phospholipid metabolism in Arabidopsis. Characterized and
RT   putative isoforms of inositol phospholipid kinase and
RT   phosphoinositide-specific phospholipase C.";
RL   Plant Physiol. 130:22-46(2002).
CC   -!- CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-
CC       phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q8L796-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8L796-2; Sequence=VSP_015946;
CC         Note=May be due to intron retention. No experimental
CC         confirmation available;
CC   -!- SIMILARITY: Contains 7 MORN repeats.
CC   -!- SIMILARITY: Contains 1 PIPK domain.
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DR   EMBL; AC012193; AAG51623.1; -; Genomic_DNA.
DR   EMBL; AY136391; AAM97057.1; -; mRNA.
DR   EMBL; BT002112; AAN72123.1; -; mRNA.
DR   IPI; IPI00518996; -.
DR   IPI; IPI00655474; -.
DR   PIR; C96807; C96807.
DR   RefSeq; NP_177897.1; -.
DR   UniGene; At.34409; -.
DR   HSSP; P78356; 1BO1.
DR   GeneID; 844110; -.
DR   GenomeReviews; CT485782_GR; AT1G77740.
DR   KEGG; ath:AT1G77740; -.
DR   NMPDR; fig|3702.1.peg.7316; -.
DR   TAIR; At1g77740; -.
DR   OMA; Q8L796; KPLIRLG.
DR   BRENDA; 2.7.1.68; 302.
DR   ArrayExpress; Q8L796; -.
DR   GermOnline; AT1G77740; Arabidopsis thaliana.
DR   GO; GO:0016308; F:1-phosphatidylinositol-4-phosphate 5-kinase...; IEA:EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046488; P:phosphatidylinositol metabolic process; IEA:InterPro.
DR   InterPro; IPR003409; MORN.
DR   InterPro; IPR017163; PIno-4-P-5_kinase_pln.
DR   InterPro; IPR002498; PInositol-4-P-5-kinase_core.
DR   InterPro; IPR016034; PInositol-4P-5-kinase_core_sub.
DR   PANTHER; PTHR23086; PIP5K; 1.
DR   Pfam; PF02493; MORN; 7.
DR   Pfam; PF01504; PIP5K; 1.
DR   PIRSF; PIRSF037274; PIP5K_plant_prd; 1.
DR   SMART; SM00698; MORN; 7.
DR   SMART; SM00330; PIPKc; 1.
DR   PROSITE; PS51455; PIPK; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; ATP-binding; Complete proteome; Kinase;
KW   Nucleotide-binding; Repeat; Transferase.
FT   CHAIN         1    754       Phosphatidylinositol-4-phosphate 5-kinase
FT                                2.
FT                                /FTId=PRO_0000185474.
FT   REPEAT       82    104       MORN 1.
FT   REPEAT      105    127       MORN 2.
FT   REPEAT      128    150       MORN 3.
FT   REPEAT      151    173       MORN 4.
FT   REPEAT      174    196       MORN 5.
FT   REPEAT      197    219       MORN 6.
FT   REPEAT      220    242       MORN 7.
FT   DOMAIN      351    750       PIPK.
FT   REGION      710    731       Activation loop (By similarity).
FT   COMPBIAS      9     14       Poly-Glu.
FT   VAR_SEQ       1    436       Missing (in isoform 2).
FT                                /FTId=VSP_015946.
SQ   SEQUENCE   754 AA;  86345 MW;  5F861AA47EEC0B33 CRC64;
     MMREPLVSEE EEEEATEVLL VEKTKLCKRR GDEEKTEERR DDLLLLALTP MVRSKSQGTT
     RRVTPTPPPV DVEKPLPNGD LYMGTFSGGF PNGSGKYLWK DGCMYEGEWK RGKASGKGKF
     SWPSGATYEG EFKSGRMEGS GTFVGVDGDT YRGSWVADRK QGHGQKRYAN GDYYEGTWRR
     NLQDGRGRYV WMNGNQYTGE WRNGVICGKG VLAWPNGNRY EGQWENGVPK GSGVFTWADG
     SSWIGSWNES SNLMRNFFDG IEKNELIVAT RKRSSVDSGA GSLTGEKIFP RICIWESDGE
     AGDITCDIVD NVEASVIYRD RISIDKDGFR QFRKNPCCFS GEAKKPGETI SKGHKKYDLM
     LNLQHGIRYS VGKHASVVRD LKQSDFDPSE KFWTRFPPEG SKTTPPHLSV DFRWKDYCPL
     VFRRLRELFT VDPADYMLAI CGNDALRELS SPGKSGSFFY LTQDDRFMIK TVKKSEVKVL
     LRMLPSYYKH VCQYENTLVT RFYGVHCIKP VGGQKTRFIV MGNLFCSEYR IQRRFDLKGS
     SHGRYTSKPE GEIDETTTLK DLDLNFAFRL QRNWYQELMT QIKRDCEFLE AERIMDYSLL
     VGVHFRDDNT GDKMGLSPFV LRSGKIESYQ SEKFMRGCRF LEAELQDMDR ILAGRKPLIR
     LGANMPARAE RMARRSDYDQ YSSGGTNYQS HGEVYEVVLY FGIIDILQDY DISKKIEHAY
     KSLQADPASI SAVDPKLYSR RFRDFISRIF IEDG
//