Reviewed, UniProtKB/Swiss-Prot Q8L796 (PI5K2_ARATH)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol-4-phosphate 5-kinase 2
      Short name=AtPIP5K2
    EC=2.7.1.68
Alternative name(s):
    1-phosphatidylinositol-4-phosphate kinase 2
    PtdIns(4)P-5-kinase 2
    Diphosphoinositide kinase 2

Gene names

Name:	PIP5K2
Ordered Locus Names:	At1g77740
ORF Names:	T32E8.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	754 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
Sequence similarities	Contains 7 MORN repeats. Contains 1 PI5K domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphatidylinositol metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-phosphatidylinositol-4-phosphate 5-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8L796-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8L796-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-436: Missing.

Note: May be due to intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 754	754	Phosphatidylinositol-4-phosphate 5-kinase 2		PRO_0000185474
Regions
	Repeat	82 – 104	23	MORN 1
	Repeat	105 – 127	23	MORN 2
	Repeat	128 – 150	23	MORN 3
	Repeat	151 – 173	23	MORN 4
	Repeat	174 – 196	23	MORN 5
	Repeat	197 – 219	23	MORN 6
	Repeat	220 – 242	23	MORN 7
	Domain	438 – 750	313	PI5K
	Region	710 – 731	22	Activation loop By similarity
	Compositional bias	9 – 14	6	Poly-Glu
Natural variations
	Alternative sequence	1 – 436	436	Missing in isoform 2.		VSP_015946

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 25, 2005. Version 2. Checksum: 5F861AA47EEC0B33	FASTA	754	86,345
	10 20 30 40 50 60 MMREPLVSEE EEEEATEVLL VEKTKLCKRR GDEEKTEERR DDLLLLALTP MVRSKSQGTT 70 80 90 100 110 120 RRVTPTPPPV DVEKPLPNGD LYMGTFSGGF PNGSGKYLWK DGCMYEGEWK RGKASGKGKF 130 140 150 160 170 180 SWPSGATYEG EFKSGRMEGS GTFVGVDGDT YRGSWVADRK QGHGQKRYAN GDYYEGTWRR 190 200 210 220 230 240 NLQDGRGRYV WMNGNQYTGE WRNGVICGKG VLAWPNGNRY EGQWENGVPK GSGVFTWADG 250 260 270 280 290 300 SSWIGSWNES SNLMRNFFDG IEKNELIVAT RKRSSVDSGA GSLTGEKIFP RICIWESDGE 310 320 330 340 350 360 AGDITCDIVD NVEASVIYRD RISIDKDGFR QFRKNPCCFS GEAKKPGETI SKGHKKYDLM 370 380 390 400 410 420 LNLQHGIRYS VGKHASVVRD LKQSDFDPSE KFWTRFPPEG SKTTPPHLSV DFRWKDYCPL 430 440 450 460 470 480 VFRRLRELFT VDPADYMLAI CGNDALRELS SPGKSGSFFY LTQDDRFMIK TVKKSEVKVL 490 500 510 520 530 540 LRMLPSYYKH VCQYENTLVT RFYGVHCIKP VGGQKTRFIV MGNLFCSEYR IQRRFDLKGS 550 560 570 580 590 600 SHGRYTSKPE GEIDETTTLK DLDLNFAFRL QRNWYQELMT QIKRDCEFLE AERIMDYSLL 610 620 630 640 650 660 VGVHFRDDNT GDKMGLSPFV LRSGKIESYQ SEKFMRGCRF LEAELQDMDR ILAGRKPLIR 670 680 690 700 710 720 LGANMPARAE RMARRSDYDQ YSSGGTNYQS HGEVYEVVLY FGIIDILQDY DISKKIEHAY 730 740 750 KSLQADPASI SAVDPKLYSR RFRDFISRIF IEDG
	Isoform 2. Checksum: BBB461A11373336C	FASTA	318	36,966
	10 20 30 40 50 60 MLAICGNDAL RELSSPGKSG SFFYLTQDDR FMIKTVKKSE VKVLLRMLPS YYKHVCQYEN 70 80 90 100 110 120 TLVTRFYGVH CIKPVGGQKT RFIVMGNLFC SEYRIQRRFD LKGSSHGRYT SKPEGEIDET 130 140 150 160 170 180 TTLKDLDLNF AFRLQRNWYQ ELMTQIKRDC EFLEAERIMD YSLLVGVHFR DDNTGDKMGL 190 200 210 220 230 240 SPFVLRSGKI ESYQSEKFMR GCRFLEAELQ DMDRILAGRK PLIRLGANMP ARAERMARRS 250 260 270 280 290 300 DYDQYSSGGT NYQSHGEVYE VVLYFGIIDI LQDYDISKKI EHAYKSLQAD PASISAVDPK 310 LYSRRFRDFI SRIFIEDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[3]	"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C." Mueller-Roeber B., Pical C. Plant Physiol. 130:22-46(2002) [PubMed: 12226484] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC012193 Genomic DNA. Translation: AAG51623.1. AY136391 mRNA. Translation: AAM97057.1. BT002112 mRNA. Translation: AAN72123.1.
IPI	IPI00518996. IPI00655474.
PIR	C96807.
RefSeq	NP_177897.1.
UniGene	At.34409
3D structure databases
HSSP	HSSP built from PDB template 1BO1 based on UniProtKB P78356.
ModBase	Search...
Genome annotation databases
GeneID	844110.
GenomeReviews	Gene locus AT1G77740 in contig CT485782_GR.
KEGG	ath:AT1G77740.
NMPDR	fig|3702.1.peg.7316.
Organism-specific databases
TAIR	At1g77740.
Phylogenomic databases
OMA	Q8L796. KPLIRLG.
Enzyme and pathway databases
BRENDA	2.7.1.68. 302.
Gene expression databases
ArrayExpress	Q8L796.
GermOnline	AT1G77740. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003409. MORN. IPR017163. PIno-4-P-5_kinase_pln. IPR002498. PInositol-4-P-5-kinase_core. IPR016034. PInositol-4P-5-kinase_core_sub. [Graphical view]
PANTHER	PTHR23086. PIP5K. 1 hit.
Pfam	PF02493. MORN. 7 hits. PF01504. PIP5K. 1 hit. [Graphical view]
PIRSF	PIRSF037274. PIP5K_plant_prd. 1 hit.
SMART	SM00698. MORN. 7 hits. SM00330. PIPKc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI5K2_ARATH

Accession

Primary (citable) accession number: Q8L796
Secondary accession number(s): Q9CA20

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)