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Q8L796 (PI5K2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol 4-phosphate 5-kinase 2

Short name=AtPIP5K2
EC=2.7.1.68
Alternative name(s):
1-phosphatidylinositol 4-phosphate kinase 2
Diphosphoinositide kinase 2
PtdIns(4)P-5-kinase 2
Gene names
Name:PIP5K2
Ordered Locus Names:At1g77740
ORF Names:T32E8.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length754 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Possesses phosphatidylinositol kinase activity in vitro. Ref.5

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Enzyme regulation

Activated by binding to RABE1D.

Subunit structure

Interacts with RABE1A, TUFA, RABE1C, RABE1D and RABE1E. Ref.5

Subcellular location

Cell membrane Ref.5.

Miscellaneous

Plants overexpressing PI5K2 exhibit severe dwarfism and are enriched in the plasma membrane of the root meristem (Ref.5).

Sequence similarities

Contains 7 MORN repeats.

Contains 1 PIPK domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8L796-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8L796-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-436: Missing.
Note: May be due to intron retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 754754Phosphatidylinositol 4-phosphate 5-kinase 2
PRO_0000185474

Regions

Repeat82 – 10423MORN 1
Repeat105 – 12723MORN 2
Repeat128 – 15023MORN 3
Repeat151 – 17323MORN 4
Repeat174 – 19623MORN 5
Repeat197 – 21923MORN 6
Repeat220 – 24223MORN 7
Domain351 – 750400PIPK
Region710 – 73122Activation loop By similarity
Compositional bias9 – 146Poly-Glu

Natural variations

Alternative sequence1 – 436436Missing in isoform 2.
VSP_015946

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 25, 2005. Version 2.
Checksum: 5F861AA47EEC0B33

FASTA75486,345
        10         20         30         40         50         60 
MMREPLVSEE EEEEATEVLL VEKTKLCKRR GDEEKTEERR DDLLLLALTP MVRSKSQGTT 

        70         80         90        100        110        120 
RRVTPTPPPV DVEKPLPNGD LYMGTFSGGF PNGSGKYLWK DGCMYEGEWK RGKASGKGKF 

       130        140        150        160        170        180 
SWPSGATYEG EFKSGRMEGS GTFVGVDGDT YRGSWVADRK QGHGQKRYAN GDYYEGTWRR 

       190        200        210        220        230        240 
NLQDGRGRYV WMNGNQYTGE WRNGVICGKG VLAWPNGNRY EGQWENGVPK GSGVFTWADG 

       250        260        270        280        290        300 
SSWIGSWNES SNLMRNFFDG IEKNELIVAT RKRSSVDSGA GSLTGEKIFP RICIWESDGE 

       310        320        330        340        350        360 
AGDITCDIVD NVEASVIYRD RISIDKDGFR QFRKNPCCFS GEAKKPGETI SKGHKKYDLM 

       370        380        390        400        410        420 
LNLQHGIRYS VGKHASVVRD LKQSDFDPSE KFWTRFPPEG SKTTPPHLSV DFRWKDYCPL 

       430        440        450        460        470        480 
VFRRLRELFT VDPADYMLAI CGNDALRELS SPGKSGSFFY LTQDDRFMIK TVKKSEVKVL 

       490        500        510        520        530        540 
LRMLPSYYKH VCQYENTLVT RFYGVHCIKP VGGQKTRFIV MGNLFCSEYR IQRRFDLKGS 

       550        560        570        580        590        600 
SHGRYTSKPE GEIDETTTLK DLDLNFAFRL QRNWYQELMT QIKRDCEFLE AERIMDYSLL 

       610        620        630        640        650        660 
VGVHFRDDNT GDKMGLSPFV LRSGKIESYQ SEKFMRGCRF LEAELQDMDR ILAGRKPLIR 

       670        680        690        700        710        720 
LGANMPARAE RMARRSDYDQ YSSGGTNYQS HGEVYEVVLY FGIIDILQDY DISKKIEHAY 

       730        740        750 
KSLQADPASI SAVDPKLYSR RFRDFISRIF IEDG 

« Hide

Isoform 2 [UniParc].

Checksum: BBB461A11373336C
Show »

FASTA31836,966

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[4]"Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
Mueller-Roeber B., Pical C.
Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[5]"Arabidopsis Rab-E GTPases exhibit a novel interaction with a plasma-membrane phosphatidylinositol-4-phosphate 5-kinase."
Camacho L., Smertenko A.P., Perez-Gomez J., Hussey P.J., Moore I.
J. Cell Sci. 122:4383-4392(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RABE1A; TUFA; RABE1C; RABE1D AND RABE1E, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC012193 Genomic DNA. Translation: AAG51623.1.
CP002684 Genomic DNA. Translation: AEE36015.1.
AY136391 mRNA. Translation: AAM97057.1.
BT002112 mRNA. Translation: AAN72123.1.
PIRC96807.
RefSeqNP_177897.1. NM_106423.2.
UniGeneAt.34409.

3D structure databases

ProteinModelPortalQ8L796.
SMRQ8L796. Positions 72-253, 355-751.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid29329. 1 interaction.
STRING3702.AT1G77740.1-P.

Proteomic databases

PRIDEQ8L796.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G77740.1; AT1G77740.1; AT1G77740. [Q8L796-1]
GeneID844110.
KEGGath:AT1G77740.

Organism-specific databases

TAIRAT1G77740.

Phylogenomic databases

eggNOGCOG4642.
HOGENOMHOG000193875.
InParanoidQ8L796.
KOK00889.
OMAWANGSRY.
PhylomeDBQ8L796.
ProtClustDBCLSN2679235.

Enzyme and pathway databases

BioCycARA:AT1G77740-MONOMER.

Gene expression databases

GenevestigatorQ8L796.

Family and domain databases

Gene3D3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
InterProIPR003409. MORN.
IPR017163. PIno-4-P-5_kinase_pln.
IPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERPTHR23086. PTHR23086. 1 hit.
PfamPF02493. MORN. 7 hits.
PF01504. PIP5K. 1 hit.
[Graphical view]
PIRSFPIRSF037274. PIP5K_plant_prd. 1 hit.
SMARTSM00698. MORN. 7 hits.
SM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEPS51455. PIPK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePI5K2_ARATH
AccessionPrimary (citable) accession number: Q8L796
Secondary accession number(s): Q9CA20
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: April 16, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names