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Q8L796

- PI5K2_ARATH

UniProt

Q8L796 - PI5K2_ARATH

Protein

Phosphatidylinositol 4-phosphate 5-kinase 2

Gene

PIP5K2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (25 Oct 2005)
      Previous versions | rss
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    Functioni

    Possesses phosphatidylinositol kinase activity in vitro.1 Publication

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

    Enzyme regulationi

    Activated by binding to RABE1D.

    GO - Molecular functioni

    1. 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: TAIR
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. developmental process involved in reproduction Source: TAIR
    2. growth Source: TAIR
    3. lateral root formation Source: TAIR
    4. phosphatidylinositol phosphorylation Source: GOC
    5. positive gravitropism Source: TAIR

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G77740-MONOMER.
    ReactomeiREACT_187642. Synthesis of PIPs at the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 4-phosphate 5-kinase 2 (EC:2.7.1.68)
    Short name:
    AtPIP5K2
    Alternative name(s):
    1-phosphatidylinositol 4-phosphate kinase 2
    Diphosphoinositide kinase 2
    PtdIns(4)P-5-kinase 2
    Gene namesi
    Name:PIP5K2
    Ordered Locus Names:At1g77740
    ORF Names:T32E8.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G77740.

    Subcellular locationi

    Cell membrane 1 Publication

    GO - Cellular componenti

    1. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 754754Phosphatidylinositol 4-phosphate 5-kinase 2PRO_0000185474Add
    BLAST

    Proteomic databases

    PRIDEiQ8L796.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8L796.

    Interactioni

    Subunit structurei

    Interacts with RABE1A, TUFA, RABE1C, RABE1D and RABE1E.1 Publication

    Protein-protein interaction databases

    BioGridi29329. 1 interaction.
    STRINGi3702.AT1G77740.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L796.
    SMRiQ8L796. Positions 407-604.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati82 – 10423MORN 1Add
    BLAST
    Repeati105 – 12723MORN 2Add
    BLAST
    Repeati128 – 15023MORN 3Add
    BLAST
    Repeati151 – 17323MORN 4Add
    BLAST
    Repeati174 – 19623MORN 5Add
    BLAST
    Repeati197 – 21923MORN 6Add
    BLAST
    Repeati220 – 24223MORN 7Add
    BLAST
    Domaini351 – 750400PIPKPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni710 – 73122Activation loopBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi9 – 146Poly-Glu

    Sequence similaritiesi

    Contains 7 MORN repeats.Curated
    Contains 1 PIPK domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG4642.
    HOGENOMiHOG000193875.
    InParanoidiQ8L796.
    KOiK00889.
    OMAiWANGSRY.
    PhylomeDBiQ8L796.

    Family and domain databases

    Gene3Di3.30.800.10. 1 hit.
    3.30.810.10. 2 hits.
    InterProiIPR003409. MORN.
    IPR017163. PIno-4-P-5_kinase_pln.
    IPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view]
    PANTHERiPTHR23086. PTHR23086. 1 hit.
    PfamiPF02493. MORN. 7 hits.
    PF01504. PIP5K. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037274. PIP5K_plant_prd. 1 hit.
    SMARTiSM00698. MORN. 7 hits.
    SM00330. PIPKc. 1 hit.
    [Graphical view]
    PROSITEiPS51455. PIPK. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8L796-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMREPLVSEE EEEEATEVLL VEKTKLCKRR GDEEKTEERR DDLLLLALTP    50
    MVRSKSQGTT RRVTPTPPPV DVEKPLPNGD LYMGTFSGGF PNGSGKYLWK 100
    DGCMYEGEWK RGKASGKGKF SWPSGATYEG EFKSGRMEGS GTFVGVDGDT 150
    YRGSWVADRK QGHGQKRYAN GDYYEGTWRR NLQDGRGRYV WMNGNQYTGE 200
    WRNGVICGKG VLAWPNGNRY EGQWENGVPK GSGVFTWADG SSWIGSWNES 250
    SNLMRNFFDG IEKNELIVAT RKRSSVDSGA GSLTGEKIFP RICIWESDGE 300
    AGDITCDIVD NVEASVIYRD RISIDKDGFR QFRKNPCCFS GEAKKPGETI 350
    SKGHKKYDLM LNLQHGIRYS VGKHASVVRD LKQSDFDPSE KFWTRFPPEG 400
    SKTTPPHLSV DFRWKDYCPL VFRRLRELFT VDPADYMLAI CGNDALRELS 450
    SPGKSGSFFY LTQDDRFMIK TVKKSEVKVL LRMLPSYYKH VCQYENTLVT 500
    RFYGVHCIKP VGGQKTRFIV MGNLFCSEYR IQRRFDLKGS SHGRYTSKPE 550
    GEIDETTTLK DLDLNFAFRL QRNWYQELMT QIKRDCEFLE AERIMDYSLL 600
    VGVHFRDDNT GDKMGLSPFV LRSGKIESYQ SEKFMRGCRF LEAELQDMDR 650
    ILAGRKPLIR LGANMPARAE RMARRSDYDQ YSSGGTNYQS HGEVYEVVLY 700
    FGIIDILQDY DISKKIEHAY KSLQADPASI SAVDPKLYSR RFRDFISRIF 750
    IEDG 754
    Length:754
    Mass (Da):86,345
    Last modified:October 25, 2005 - v2
    Checksum:i5F861AA47EEC0B33
    GO
    Isoform 2 (identifier: Q8L796-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-436: Missing.

    Note: May be due to intron retention. No experimental confirmation available.

    Show »
    Length:318
    Mass (Da):36,966
    Checksum:iBBB461A11373336C
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 436436Missing in isoform 2. 1 PublicationVSP_015946Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC012193 Genomic DNA. Translation: AAG51623.1.
    CP002684 Genomic DNA. Translation: AEE36015.1.
    AY136391 mRNA. Translation: AAM97057.1.
    BT002112 mRNA. Translation: AAN72123.1.
    PIRiC96807.
    RefSeqiNP_177897.1. NM_106423.2. [Q8L796-1]
    UniGeneiAt.34409.

    Genome annotation databases

    EnsemblPlantsiAT1G77740.1; AT1G77740.1; AT1G77740. [Q8L796-1]
    GeneIDi844110.
    KEGGiath:AT1G77740.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC012193 Genomic DNA. Translation: AAG51623.1 .
    CP002684 Genomic DNA. Translation: AEE36015.1 .
    AY136391 mRNA. Translation: AAM97057.1 .
    BT002112 mRNA. Translation: AAN72123.1 .
    PIRi C96807.
    RefSeqi NP_177897.1. NM_106423.2. [Q8L796-1 ]
    UniGenei At.34409.

    3D structure databases

    ProteinModelPortali Q8L796.
    SMRi Q8L796. Positions 407-604.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 29329. 1 interaction.
    STRINGi 3702.AT1G77740.1-P.

    Proteomic databases

    PRIDEi Q8L796.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G77740.1 ; AT1G77740.1 ; AT1G77740 . [Q8L796-1 ]
    GeneIDi 844110.
    KEGGi ath:AT1G77740.

    Organism-specific databases

    TAIRi AT1G77740.

    Phylogenomic databases

    eggNOGi COG4642.
    HOGENOMi HOG000193875.
    InParanoidi Q8L796.
    KOi K00889.
    OMAi WANGSRY.
    PhylomeDBi Q8L796.

    Enzyme and pathway databases

    BioCyci ARA:AT1G77740-MONOMER.
    Reactomei REACT_187642. Synthesis of PIPs at the plasma membrane.

    Gene expression databases

    Genevestigatori Q8L796.

    Family and domain databases

    Gene3Di 3.30.800.10. 1 hit.
    3.30.810.10. 2 hits.
    InterProi IPR003409. MORN.
    IPR017163. PIno-4-P-5_kinase_pln.
    IPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view ]
    PANTHERi PTHR23086. PTHR23086. 1 hit.
    Pfami PF02493. MORN. 7 hits.
    PF01504. PIP5K. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037274. PIP5K_plant_prd. 1 hit.
    SMARTi SM00698. MORN. 7 hits.
    SM00330. PIPKc. 1 hit.
    [Graphical view ]
    PROSITEi PS51455. PIPK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    4. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
      Mueller-Roeber B., Pical C.
      Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. "Arabidopsis Rab-E GTPases exhibit a novel interaction with a plasma-membrane phosphatidylinositol-4-phosphate 5-kinase."
      Camacho L., Smertenko A.P., Perez-Gomez J., Hussey P.J., Moore I.
      J. Cell Sci. 122:4383-4392(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RABE1A; TUFA; RABE1C; RABE1D AND RABE1E, INDUCTION.

    Entry informationi

    Entry nameiPI5K2_ARATH
    AccessioniPrimary (citable) accession number: Q8L796
    Secondary accession number(s): Q9CA20
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: October 25, 2005
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Plants overexpressing PI5K2 exhibit severe dwarfism and are enriched in the plasma membrane of the root meristem.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3