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Protein

Phosphatidylinositol 4-phosphate 5-kinase 2

Gene

PIP5K2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possesses phosphatidylinositol kinase activity in vitro.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Enzyme regulationi

Activated by binding to RABE1D.

GO - Molecular functioni

  • 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • developmental process involved in reproduction Source: TAIR
  • growth Source: TAIR
  • lateral root formation Source: TAIR
  • phosphatidylinositol phosphorylation Source: GOC
  • positive gravitropism Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G77740-MONOMER.
ReactomeiREACT_345128. Synthesis of PIPs at the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-phosphate 5-kinase 2 (EC:2.7.1.68)
Short name:
AtPIP5K2
Alternative name(s):
1-phosphatidylinositol 4-phosphate kinase 2
Diphosphoinositide kinase 2
PtdIns(4)P-5-kinase 2
Gene namesi
Name:PIP5K2
Ordered Locus Names:At1g77740
ORF Names:T32E8.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G77740.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Phosphatidylinositol 4-phosphate 5-kinase 2PRO_0000185474Add
BLAST

Proteomic databases

PRIDEiQ8L796.

Interactioni

Subunit structurei

Interacts with RABE1A, TUFA, RABE1C, RABE1D and RABE1E.1 Publication

Protein-protein interaction databases

BioGridi29329. 1 interaction.
STRINGi3702.AT1G77740.1.

Structurei

3D structure databases

ProteinModelPortaliQ8L796.
SMRiQ8L796. Positions 407-604.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati82 – 10423MORN 1Add
BLAST
Repeati105 – 12723MORN 2Add
BLAST
Repeati128 – 15023MORN 3Add
BLAST
Repeati151 – 17323MORN 4Add
BLAST
Repeati174 – 19623MORN 5Add
BLAST
Repeati197 – 21923MORN 6Add
BLAST
Repeati220 – 24223MORN 7Add
BLAST
Domaini351 – 750400PIPKPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni710 – 73122Activation loopBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi9 – 146Poly-Glu

Sequence similaritiesi

Contains 7 MORN repeats.Curated
Contains 1 PIPK domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG4642.
HOGENOMiHOG000193875.
InParanoidiQ8L796.
KOiK00889.
OMAiQIPEIST.
PhylomeDBiQ8L796.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
InterProiIPR003409. MORN.
IPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiPF02493. MORN. 7 hits.
PF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00698. MORN. 7 hits.
SM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEiPS51455. PIPK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8L796-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMREPLVSEE EEEEATEVLL VEKTKLCKRR GDEEKTEERR DDLLLLALTP
60 70 80 90 100
MVRSKSQGTT RRVTPTPPPV DVEKPLPNGD LYMGTFSGGF PNGSGKYLWK
110 120 130 140 150
DGCMYEGEWK RGKASGKGKF SWPSGATYEG EFKSGRMEGS GTFVGVDGDT
160 170 180 190 200
YRGSWVADRK QGHGQKRYAN GDYYEGTWRR NLQDGRGRYV WMNGNQYTGE
210 220 230 240 250
WRNGVICGKG VLAWPNGNRY EGQWENGVPK GSGVFTWADG SSWIGSWNES
260 270 280 290 300
SNLMRNFFDG IEKNELIVAT RKRSSVDSGA GSLTGEKIFP RICIWESDGE
310 320 330 340 350
AGDITCDIVD NVEASVIYRD RISIDKDGFR QFRKNPCCFS GEAKKPGETI
360 370 380 390 400
SKGHKKYDLM LNLQHGIRYS VGKHASVVRD LKQSDFDPSE KFWTRFPPEG
410 420 430 440 450
SKTTPPHLSV DFRWKDYCPL VFRRLRELFT VDPADYMLAI CGNDALRELS
460 470 480 490 500
SPGKSGSFFY LTQDDRFMIK TVKKSEVKVL LRMLPSYYKH VCQYENTLVT
510 520 530 540 550
RFYGVHCIKP VGGQKTRFIV MGNLFCSEYR IQRRFDLKGS SHGRYTSKPE
560 570 580 590 600
GEIDETTTLK DLDLNFAFRL QRNWYQELMT QIKRDCEFLE AERIMDYSLL
610 620 630 640 650
VGVHFRDDNT GDKMGLSPFV LRSGKIESYQ SEKFMRGCRF LEAELQDMDR
660 670 680 690 700
ILAGRKPLIR LGANMPARAE RMARRSDYDQ YSSGGTNYQS HGEVYEVVLY
710 720 730 740 750
FGIIDILQDY DISKKIEHAY KSLQADPASI SAVDPKLYSR RFRDFISRIF

IEDG
Length:754
Mass (Da):86,345
Last modified:October 25, 2005 - v2
Checksum:i5F861AA47EEC0B33
GO
Isoform 2 (identifier: Q8L796-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-436: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:318
Mass (Da):36,966
Checksum:iBBB461A11373336C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 436436Missing in isoform 2. 1 PublicationVSP_015946Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012193 Genomic DNA. Translation: AAG51623.1.
CP002684 Genomic DNA. Translation: AEE36015.1.
AY136391 mRNA. Translation: AAM97057.1.
BT002112 mRNA. Translation: AAN72123.1.
PIRiC96807.
RefSeqiNP_177897.1. NM_106423.2. [Q8L796-1]
UniGeneiAt.34409.

Genome annotation databases

EnsemblPlantsiAT1G77740.1; AT1G77740.1; AT1G77740. [Q8L796-1]
GeneIDi844110.
KEGGiath:AT1G77740.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012193 Genomic DNA. Translation: AAG51623.1.
CP002684 Genomic DNA. Translation: AEE36015.1.
AY136391 mRNA. Translation: AAM97057.1.
BT002112 mRNA. Translation: AAN72123.1.
PIRiC96807.
RefSeqiNP_177897.1. NM_106423.2. [Q8L796-1]
UniGeneiAt.34409.

3D structure databases

ProteinModelPortaliQ8L796.
SMRiQ8L796. Positions 407-604.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29329. 1 interaction.
STRINGi3702.AT1G77740.1.

Proteomic databases

PRIDEiQ8L796.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G77740.1; AT1G77740.1; AT1G77740. [Q8L796-1]
GeneIDi844110.
KEGGiath:AT1G77740.

Organism-specific databases

TAIRiAT1G77740.

Phylogenomic databases

eggNOGiCOG4642.
HOGENOMiHOG000193875.
InParanoidiQ8L796.
KOiK00889.
OMAiQIPEIST.
PhylomeDBiQ8L796.

Enzyme and pathway databases

BioCyciARA:AT1G77740-MONOMER.
ReactomeiREACT_345128. Synthesis of PIPs at the plasma membrane.

Miscellaneous databases

PROiQ8L796.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
InterProiIPR003409. MORN.
IPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiPF02493. MORN. 7 hits.
PF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00698. MORN. 7 hits.
SM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEiPS51455. PIPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
    Mueller-Roeber B., Pical C.
    Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. "Arabidopsis Rab-E GTPases exhibit a novel interaction with a plasma-membrane phosphatidylinositol-4-phosphate 5-kinase."
    Camacho L., Smertenko A.P., Perez-Gomez J., Hussey P.J., Moore I.
    J. Cell Sci. 122:4383-4392(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RABE1A; TUFA; RABE1C; RABE1D AND RABE1E, INDUCTION.

Entry informationi

Entry nameiPI5K2_ARATH
AccessioniPrimary (citable) accession number: Q8L796
Secondary accession number(s): Q9CA20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: July 22, 2015
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing PI5K2 exhibit severe dwarfism and are enriched in the plasma membrane of the root meristem.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.