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Protein

Phosphatidylinositol 4-phosphate 5-kinase 2

Gene

PIP5K2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possesses phosphatidylinositol kinase activity in vitro.1 Publication

Miscellaneous

Plants overexpressing PI5K2 exhibit severe dwarfism and are enriched in the plasma membrane of the root meristem.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Enzyme regulationi

Activated by binding to RABE1D.

GO - Molecular functioni

  • 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • developmental process involved in reproduction Source: TAIR
  • growth Source: TAIR
  • lateral root formation Source: TAIR
  • positive gravitropism Source: TAIR

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G77740-MONOMER.
ReactomeiR-ATH-1660499. Synthesis of PIPs at the plasma membrane.
R-ATH-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-ATH-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-phosphate 5-kinase 2 (EC:2.7.1.68)
Short name:
AtPIP5K2
Alternative name(s):
1-phosphatidylinositol 4-phosphate kinase 2
Diphosphoinositide kinase 2
PtdIns(4)P-5-kinase 2
Gene namesi
Name:PIP5K2
Ordered Locus Names:At1g77740
ORF Names:T32E8.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G77740.
TAIRilocus:2203211. AT1G77740.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001854741 – 754Phosphatidylinositol 4-phosphate 5-kinase 2Add BLAST754

Proteomic databases

PaxDbiQ8L796.
PRIDEiQ8L796.

PTM databases

iPTMnetiQ8L796.

Expressioni

Gene expression databases

ExpressionAtlasiQ8L796. baseline and differential.
GenevisibleiQ8L796. AT.

Interactioni

Subunit structurei

Interacts with RABE1A, TUFA, RABE1C, RABE1D and RABE1E.1 Publication

Protein-protein interaction databases

BioGridi29329. 1 interactor.
STRINGi3702.AT1G77740.1.

Structurei

3D structure databases

ProteinModelPortaliQ8L796.
SMRiQ8L796.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati82 – 104MORN 1Add BLAST23
Repeati105 – 127MORN 2Add BLAST23
Repeati128 – 150MORN 3Add BLAST23
Repeati151 – 173MORN 4Add BLAST23
Repeati174 – 196MORN 5Add BLAST23
Repeati197 – 219MORN 6Add BLAST23
Repeati220 – 242MORN 7Add BLAST23
Domaini351 – 750PIPKPROSITE-ProRule annotationAdd BLAST400

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni710 – 731Activation loopBy similarityAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 14Poly-Glu6

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0229. Eukaryota.
COG4642. LUCA.
COG5253. LUCA.
HOGENOMiHOG000193875.
InParanoidiQ8L796.
KOiK00889.
OMAiKIFPRIC.
OrthoDBiEOG0936025Q.
PhylomeDBiQ8L796.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 1 hit.
InterProiView protein in InterPro
IPR003409. MORN.
IPR017163. PIno-4-P-5_kinase_pln.
IPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiView protein in Pfam
PF02493. MORN. 7 hits.
PF01504. PIP5K. 1 hit.
PIRSFiPIRSF037274. PIP5K_plant_prd. 1 hit.
SMARTiView protein in SMART
SM00698. MORN. 7 hits.
SM00330. PIPKc. 1 hit.
PROSITEiView protein in PROSITE
PS51455. PIPK. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8L796-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMREPLVSEE EEEEATEVLL VEKTKLCKRR GDEEKTEERR DDLLLLALTP
60 70 80 90 100
MVRSKSQGTT RRVTPTPPPV DVEKPLPNGD LYMGTFSGGF PNGSGKYLWK
110 120 130 140 150
DGCMYEGEWK RGKASGKGKF SWPSGATYEG EFKSGRMEGS GTFVGVDGDT
160 170 180 190 200
YRGSWVADRK QGHGQKRYAN GDYYEGTWRR NLQDGRGRYV WMNGNQYTGE
210 220 230 240 250
WRNGVICGKG VLAWPNGNRY EGQWENGVPK GSGVFTWADG SSWIGSWNES
260 270 280 290 300
SNLMRNFFDG IEKNELIVAT RKRSSVDSGA GSLTGEKIFP RICIWESDGE
310 320 330 340 350
AGDITCDIVD NVEASVIYRD RISIDKDGFR QFRKNPCCFS GEAKKPGETI
360 370 380 390 400
SKGHKKYDLM LNLQHGIRYS VGKHASVVRD LKQSDFDPSE KFWTRFPPEG
410 420 430 440 450
SKTTPPHLSV DFRWKDYCPL VFRRLRELFT VDPADYMLAI CGNDALRELS
460 470 480 490 500
SPGKSGSFFY LTQDDRFMIK TVKKSEVKVL LRMLPSYYKH VCQYENTLVT
510 520 530 540 550
RFYGVHCIKP VGGQKTRFIV MGNLFCSEYR IQRRFDLKGS SHGRYTSKPE
560 570 580 590 600
GEIDETTTLK DLDLNFAFRL QRNWYQELMT QIKRDCEFLE AERIMDYSLL
610 620 630 640 650
VGVHFRDDNT GDKMGLSPFV LRSGKIESYQ SEKFMRGCRF LEAELQDMDR
660 670 680 690 700
ILAGRKPLIR LGANMPARAE RMARRSDYDQ YSSGGTNYQS HGEVYEVVLY
710 720 730 740 750
FGIIDILQDY DISKKIEHAY KSLQADPASI SAVDPKLYSR RFRDFISRIF

IEDG
Length:754
Mass (Da):86,345
Last modified:October 25, 2005 - v2
Checksum:i5F861AA47EEC0B33
GO
Isoform 2 (identifier: Q8L796-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-436: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:318
Mass (Da):36,966
Checksum:iBBB461A11373336C
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0159461 – 436Missing in isoform 2. 1 PublicationAdd BLAST436

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012193 Genomic DNA. Translation: AAG51623.1.
CP002684 Genomic DNA. Translation: AEE36015.1.
CP002684 Genomic DNA. Translation: ANM59859.1.
AY136391 mRNA. Translation: AAM97057.1.
BT002112 mRNA. Translation: AAN72123.1.
PIRiC96807.
RefSeqiNP_001319397.1. NM_001334792.1. [Q8L796-1]
NP_177897.1. NM_106423.2. [Q8L796-1]
UniGeneiAt.34409.

Genome annotation databases

EnsemblPlantsiAT1G77740.1; AT1G77740.1; AT1G77740. [Q8L796-1]
AT1G77740.2; AT1G77740.2; AT1G77740. [Q8L796-1]
GeneIDi844110.
GrameneiAT1G77740.1; AT1G77740.1; AT1G77740.
AT1G77740.2; AT1G77740.2; AT1G77740.
KEGGiath:AT1G77740.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPI5K2_ARATH
AccessioniPrimary (citable) accession number: Q8L796
Secondary accession number(s): Q9CA20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: August 30, 2017
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names