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Protein

Glycine--tRNA ligase, chloroplastic/mitochondrial 2

Gene

EDD1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.By similarity

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei513SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi589 – 596ATPBy similarity8
Nucleotide bindingi619 – 624ATPBy similarity6
Nucleotide bindingi744 – 745ATPBy similarity2
Nucleotide bindingi859 – 862ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • glycyl-tRNA aminoacylation Source: TAIR
  • regulation of embryonic development Source: TAIR

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.14 399

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligase, chloroplastic/mitochondrial 2Curated (EC:6.1.1.141 Publication)
Alternative name(s):
Glycyl-tRNA synthetase 21 Publication
Short name:
GlyRS-21 Publication
Protein EMBRYO-DEFECTIVE-DEVELOPMENT 11 Publication
Gene namesi
Name:EDD11 Publication
Ordered Locus Names:At3g48110
ORF Names:T17F15.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G48110
TAIRilocus:2097885 AT3G48110

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryo defective. Developmental arrest of the embryo between the globular and heart stages (PubMed:9707529). Plants with partial loss of EDD1 display changes in patterning of margin and distal regions of leaves (PubMed:22791832).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50Chloroplast and mitochondrion1 PublicationAdd BLAST50
ChainiPRO_000004601751 – 1067Glycine--tRNA ligase, chloroplastic/mitochondrial 2CuratedAdd BLAST1017

Proteomic databases

PaxDbiQ8L785
PRIDEiQ8L785

PTM databases

iPTMnetiQ8L785

Expressioni

Gene expression databases

ExpressionAtlasiQ8L785 baseline and differential
GenevisibleiQ8L785 AT

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT3G48110.1

Structurei

3D structure databases

ProteinModelPortaliQ8L785
SMRiQ8L785
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni624 – 628Substrate bindingBy similarity5
Regioni855 – 859Substrate bindingBy similarity5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IHAC Eukaryota
COG0751 LUCA
COG0752 LUCA
HOGENOMiHOG000264303
InParanoidiQ8L785
KOiK14164
OMAiKENQRYF
OrthoDBiEOG093600TZ
PhylomeDBiQ8L785

Family and domain databases

CDDicd00733 GlyRS_alpha_core, 1 hit
HAMAPiMF_00254 Gly_tRNA_synth_alpha, 1 hit
MF_00255 Gly_tRNA_synth_beta, 1 hit
InterProiView protein in InterPro
IPR015944 Gly-tRNA-synth_bsu
IPR006194 Gly-tRNA-synth_heterodimer
IPR002310 Gly-tRNA_ligase_asu
PANTHERiPTHR30075 PTHR30075, 1 hit
PfamiView protein in Pfam
PF02091 tRNA-synt_2e, 1 hit
PF02092 tRNA_synt_2f, 1 hit
PRINTSiPR01044 TRNASYNTHGA
TIGRFAMsiTIGR00388 glyQ, 1 hit
TIGR00211 glyS, 1 hit
PROSITEiView protein in PROSITE
PS50861 AA_TRNA_LIGASE_II_GLYAB, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8L785-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAILHFSLPL IVSFLRPHAS PRFFLLPRSL SQSPFLSRRR FHRTSAVSSA
60 70 80 90 100
AVHHQSYRNP DDDVTRAVSV PTFQQAIQRL QEYWASVGCA VMQPSNTEVG
110 120 130 140 150
AGTMNPCTFL RVLGPEPWNV AYVEPSIRPD DSRYGENPNR LQRHTQFQVI
160 170 180 190 200
LKPDPGNSQQ LFINSLSALG IDVTAHDIRF VEDNWESPVL GAWGLGWEIW
210 220 230 240 250
MDGMEITQFT YFQQAGSLPL SPVSVEITYG LERIIMLLQE VDHFKKILYA
260 270 280 290 300
DGITYGELFL ENEKEMSSYY LEHASVDRLQ KHFDYFDEEA RSLLALGLPI
310 320 330 340 350
PAYDQLLKTS HAFNILDARG FIGVTERARY FGRMRSLARQ CAQLWLATRE
360 370 380 390 400
SLGHPLGVAS EPVPPVCHRA ALEKVAEKVS EDPRSFIIEI GTEEMPPQDV
410 420 430 440 450
INASEQLRVL VLELLENQRL RHGAVKAFGT PRRLVVLVDA MSSKQLEEEV
460 470 480 490 500
EVRGPPASKA FDDEGNPTKA AEGFSRRYGV PLEKLYRKVS GKTEYVHARV
510 520 530 540 550
TEPARLALEV LSEDLPGILA KISFPKSMRW NSSVMFSRPI RWVMALHGDL
560 570 580 590 600
VVPFSFAGIS SGNVSCGLRN TASASLLVQN AESYEDTMRN SGINIEIEER
610 620 630 640 650
KKIILEKSNA LAKSVSGRLV VPQNLLNEVA NLVEAPVPLI GKFKESFLEL
660 670 680 690 700
PEELLTIVMQ KHQKYFSIID ESGQLLPYFI AVANGAINED VVKKGNEAVL
710 720 730 740 750
RARYEDAKFF YEVDTRKRFS EFRDQLQGIL FHEKLGTMLD KMNRLKKMVS
760 770 780 790 800
KLCLALKIDE DLLPVVEDAA SLAMSDLATA VVTEFTALSG IMARHYALRD
810 820 830 840 850
GYSEQIAEAL LEITLPRFSG DVIPKTDAGM VLAIGDRLDS LVGLFAAGCQ
860 870 880 890 900
PSSTNDPFGL RRISYGLVQI LVEKDKNVNF KRVLELAASV QPTKVEANTV
910 920 930 940 950
EDVYQFVTRR LEQLLVDNGV SPEVVRSVLA ERGNNPCLAA RTAYKTEKLS
960 970 980 990 1000
KGEMFPKIVE AYSRPTRIVR GKDVGVGVEV DENAFETPQE RTLWSTYTSI
1010 1020 1030 1040 1050
KDRIHTGIEI EDFTEISMQL VEPLEDFFNN VFVMVEEERV RKNRLALLNN
1060
IANLPKGVID LSFLPGF
Length:1,067
Mass (Da):119,773
Last modified:October 1, 2002 - v1
Checksum:iE2F0574F1F019493
GO
Isoform 2 (identifier: Q8L785-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1035-1035: Missing.

Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.
Show »
Length:1,066
Mass (Da):119,674
Checksum:iB8DE160BABC8A51D
GO

Sequence cautioni

The sequence CAB41128 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti336S → T in CAA05843 (PubMed:9707529).Curated1
Sequence conflicti535M → I in CAA05843 (PubMed:9707529).Curated1
Sequence conflicti616S → N in CAA05843 (PubMed:9707529).Curated1
Sequence conflicti893T → R in CAA05843 (PubMed:9707529).Curated1
Sequence conflicti1012D → E in CAA05843 (PubMed:9707529).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0180981035Missing in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ003069 mRNA Translation: CAA05843.1
AL049658 Genomic DNA Translation: CAB41128.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE78369.1
AY136413 mRNA Translation: AAM97079.1
BT012191 mRNA Translation: AAS76678.1
PIRiT06672
T48850
RefSeqiNP_190394.3, NM_114680.4 [Q8L785-1]
UniGeneiAt.22692

Genome annotation databases

EnsemblPlantsiAT3G48110.1; AT3G48110.1; AT3G48110 [Q8L785-1]
GeneIDi823966
GrameneiAT3G48110.1; AT3G48110.1; AT3G48110 [Q8L785-1]
KEGGiath:AT3G48110

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSYGM2_ARATH
AccessioniPrimary (citable) accession number: Q8L785
Secondary accession number(s): O23150, Q9SU73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2002
Last modified: April 25, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health