Cellulose synthase A catalytic subunit 5 [UDP-forming] - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot Q8L778 (CESA5_ARATH)

Last modified June 16, 2009. Version 51. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Cellulose synthase A catalytic subunit 5 [UDP-forming]
      Short name=AtCesA5
    EC=2.4.1.12

Gene names

Name:	CESA5
Ordered Locus Names:	At5g09870
ORF Names:	MYH9.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	1069 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation By similarity.
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Glycan metabolism; plant cellulose biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed in young plants, stems and flowers. Ref.4
Developmental stage	Expressed at low levels in embryos. Ref.4
Miscellaneous	Partially redundant with CESA6.
Sequence similarities	Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily. Contains 1 RING-type zinc finger.

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Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Cellulose biosynthesis
Cellular component	Cell membrane Membrane
Domain	Coiled coil Transmembrane Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1069

1069

Cellulose synthase A catalytic subunit 5 [UDP-forming]

PRO_0000166371

Regions

Topological domain

1 – 265

265

Cytoplasmic Potential

Transmembrane

266 – 286

Potential

Topological domain

287 – 288

Extracellular Potential

Transmembrane

289 – 309

Potential

Topological domain

310 – 853

544

Cytoplasmic Potential

Transmembrane

854 – 874

Potential

Topological domain

875 – 879

Extracellular Potential

Transmembrane

880 – 900

Potential

Topological domain

901 – 915

Cytoplasmic Potential

Transmembrane

916 – 936

Potential

Topological domain

937 – 965

Extracellular Potential

Transmembrane

966 – 986

Potential

Topological domain

987 – 997

Cytoplasmic Potential

Transmembrane

998 – 1018

Potential

Topological domain

1019 – 1027

Extracellular Potential

Transmembrane

1028 – 1048

Potential

Topological domain

1049 – 1069

Cytoplasmic Potential

Zinc finger

39 – 85

RING-type; degenerate

Coiled coil

438 – 464

Potential

Compositional bias

637 – 673

Lys-rich

Compositional bias

646 – 657

Cys-rich

Sites

Active site

384

Potential

Active site

770

Potential

Metal binding

Zinc 1 By similarity

Metal binding

Zinc 1 By similarity

Metal binding

Zinc 2 By similarity

Metal binding

Zinc 2 By similarity

Metal binding

Zinc 1 By similarity

Metal binding

Zinc 1 By similarity

Metal binding

Zinc 2 By similarity

Metal binding

Zinc 2 By similarity

Binding site

550

Substrate Potential

Binding site

552

Substrate Potential

Amino acid modifications

Modified residue

229

Phosphoserine Ref.5 Ref.6

Modified residue

230

Phosphoserine Ref.5 Ref.6

Glycosylation

943

N-linked (GlcNAc...) Potential

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Sequences

Sequence

Length

Mass (Da)

Tools

Q8L778-1 [UniParc].

Last modified August 30, 2005. Version 2.
Checksum: D03ED5C578DB3E7C
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FASTA

1,069

120,862

        10         20         30         40         50         60 
MNTGGRLIAG SHNRNEFVLI NADESARIRS VEELSGQTCQ ICGDEIELSV DGESFVACNE 

        70         80         90        100        110        120 
CAFPVCRPCY EYERREGNQS CPQCKTRYKR IKGSPRVEGD EEDDGIDDLD FEFDYSRSGL 

       130        140        150        160        170        180 
ESETFSRRNS EFDLASAPPG SQIPLLTYGE EDVEISSDSH ALIVSPSPGH IHRVHQPHFP 

       190        200        210        220        230        240 
DPAAHPRPMV PQKDLAVYGY GSVAWKDRME EWKRKQNEKY QVVKHDGDSS LGDGDDADIP 

       250        260        270        280        290        300 
MMDEGRQPLS RKVPIKSSKI NPYRMLIVLR LVILGLFFHY RILHPVNDAY ALWLISVICE 

       310        320        330        340        350        360 
IWFAVSWVLD QFPKWYPIER ETYLDRLSLR YEKEGKPSEL AGVDVFVSTV DPMKEPPLIT 

       370        380        390        400        410        420 
ANTVLSILAV DYPVDRVACY VSDDGAAMLT FEALSETAEF ARKWVPFCKK YTIEPRAPEW 

       430        440        450        460        470        480 
YFCHKMDYLK NKVHPAFVRE RRAMKRDYEE FKVKINALVA TAQKVPEEGW TMQDGTPWPG 

       490        500        510        520        530        540 
NNVRDHPGMI QVFLGNNGVR DVENNELPRL VYVSREKRPG FDHHKKAGAM NSLIRVSGVL 

       550        560        570        580        590        600 
SNAPYLLNVD CDHYINNSKA LREAMCFMMD PQSGKKICYV QFPQRFDGID KSDRYSNRNV 

       610        620        630        640        650        660 
VFFDINMKGL DGLQGPIYVG TGCVFRRQAL YGFDAPKKKK TKRMTCNCWP KWCLFCCGLR 

       670        680        690        700        710        720 
KNRKSKTTDK KKKNREASKQ IHALENIEEG TKGTNDAAKS PEAAQLKLEK KFGQSPVFVA 

       730        740        750        760        770        780 
SAGMENGGLA RNASPASLLR EAIQVISCGY EDKTEWGKEI GWIYGSVTED ILTGFKMHSH 

       790        800        810        820        830        840 
GWRSVYCTPK IPAFKGSAPI NLSDRLHQVL RWALGSVEIF LSRHCPIWYG YGGGLKWLER 

       850        860        870        880        890        900 
LSYINSVVYP WTSIPLLVYC SLPAICLLTG KFIVPEISNY ASILFMALFG SIAVTGILEM 

       910        920        930        940        950        960 
QWGKVGIDDW WRNEQFWVIG GVSAHLFALF QGLLKVLAGV ETNFTVTSKA ADDGEFSELY 

       970        980        990       1000       1010       1020 
IFKWTSLLIP PTTLLIINVI GVIVGISDAI SNGYDSWGPL FGRLFFAFWV ILHLYPFLKG 

      1030       1040       1050       1060 
LLGKQDRMPT IILVWSILLA SILTLLWVRV NPFVAKGGPI LEICGLDCL

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References

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[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones." Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S. DNA Res. 5:379-391(1998) [PubMed: 10048488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 671-1069. Strain: cv. Columbia.
[3]	"Higher plant cellulose synthases." Richmond T. Genome Biol. 1:REVIEWS3001.1-REVIEWS3001.6(2000) [PubMed: 11178255] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[4]	"Genetic complexity of cellulose synthase A gene function in Arabidopsis embryogenesis." Beeckman T., Przemeck G.K.H., Stamatiou G., Lau R., Terryn N., De Rycke R., Inze D., Berleth T. Plant Physiol. 130:1883-1893(2002) [PubMed: 12481071] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]	"Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database." Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C. Plant Cell 16:2394-2405(2004) [PubMed: 15308754] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229 AND SER-230, MASS SPECTROMETRY.
[6]	"Identification of cellulose synthase AtCesA7 (IRX3) in vivo phosphorylation sites -- a potential role in regulating protein degradation." Taylor N.G. Plant Mol. Biol. 64:161-171(2007) [PubMed: 17427041] [Abstract] Cited for: PHOSPHORYLATION AT SER-229 AND SER-230, MASS SPECTROMETRY.
[7]	"Organization of cellulose synthase complexes involved in primary cell wall synthesis in Arabidopsis thaliana." Desprez T., Juraniec M., Crowell E.F., Jouy H., Pochylova Z., Parcy F., Hoefte H., Gonneau M., Vernhettes S. Proc. Natl. Acad. Sci. U.S.A. 104:15572-15577(2007) [PubMed: 17878303] [Abstract] Cited for: FUNCTION.

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Cross-references

Sequence databases
	AB016893 Genomic DNA. Translation: BAB09408.1. AY136423 mRNA. Translation: AAM97089.1. Different initiation. BT008832 mRNA. Translation: AAP68271.1. Different initiation.
IPI	IPI00524713.
RefSeq	NP_196549.1.
UniGene	At.27671
3D structure databases
SMR	Q8L778. Positions 29-97.
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	830847.
GenomeReviews	Gene locus AT5G09870 in contig BA000015_GR.
KEGG	ath:AT5G09870.
NMPDR	fig\|3702.1.peg.23111.
Organism-specific databases
GeneFarm	5088. 484.
TAIR	At5g09870.
Enzyme and pathway databases
BRENDA	2.4.1.12. 302.
Gene expression databases
ArrayExpress	Q8L778.
GermOnline	AT5G09870. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005150. Cellulose_synth. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF03552. Cellulose_synt. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

CESA5_ARATH

Accession

Primary (citable) accession number: Q8L778
Secondary accession number(s): Q9FIB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	June 16, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents