Q8L762 (BAM6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-amylase 6
EC=3.2.1.2

Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Beta-amylase 5

Gene names

Name:	BAM6
Synonyms:	BMY5
Ordered Locus Names:	At2g32290
ORF Names:	T32F6.19

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 14 family.
Sequence caution	The sequence AAC69949.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from direct assay. Source: TAIR
Molecular function	beta-amylase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	Beta-amylase 6		PRO_0000393420
Regions
	Region	448 – 449	2	Substrate binding By similarity
Sites
	Active site	252	1	Proton donor By similarity
	Active site	447	1	Proton acceptor By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	159	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	362	1	Substrate By similarity
	Binding site	367	1	Substrate By similarity
	Binding site	409	1	Substrate By similarity
	Binding site	485	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8L762 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 3538C82F34A27554	FASTA	577	66,568
	10 20 30 40 50 60 MTSVLGMMNP NLINGRNLHK GSSIFVQDKE TKKRVQWRLS IKEGSLRTHQ ATASSATEPK 70 80 90 100 110 120 ATEFNTTTYE DKMLTNYVPV YVMLQLGVIT NDNVLENEES LKKQLKKLKQ SQVDGVMVDV 130 140 150 160 170 180 WWGIVESKGP KQYQWSAYRN LFAIVQSFGL KLQAIMSFHR CGGNIGDDVN IPIPKWVLEI 190 200 210 220 230 240 GDSNPDIFYT NKSGNRNKEC LSLSVDNLSL FRGRTAVEMY RDYMKSFREN MEDFISSGVI 250 260 270 280 290 300 IDIEVGLGPA GELRYPSYSE TQGWVFPGIG EFQCYDKYLR SDYEEEVRRI GHPEWKLPEN 310 320 330 340 350 360 AGEYNSVPGE TEFFEYSNGT YLKEEGNFFL SWYSKKLLLH GDQILDEANK VFLGCKLKIA 370 380 390 400 410 420 AKVSGIHWWY KTESHAAELT AGYYNLKNRD GYRAIAKIMR RHHAILNFTC LEMKNTEQPA 430 440 450 460 470 480 KAKSGPQELV QQVLSSGWRE GIEVAGENAL PRFDRNGYNQ IILNARPNGV NQDGKPRMFG 490 500 510 520 530 540 FTYLRLSDKL LNEPNFSTFK MFLKRMHANQ EYCSEPERYN HELLPLERSR NDESLEMFME 550 560 570 ETEPFDPFPW LDETDMSIRP FESVLSLLRS TFLRKKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts." Fulton D.C., Stettler M., Mettler T., Vaughan C.K., Li J., Francisco P., Gil M., Reinhold H., Eicke S., Messerli G., Dorken G., Halliday K., Smith A.M., Smith S.M., Zeeman S.C. Plant Cell 20:1040-1058(2008) [PubMed: 18390594] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005700 Genomic DNA. Translation: AAC69949.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC08663.1. AY136463 mRNA. Translation: AAM97128.1.
IPI	IPI00524134.
PIR	C84731.
RefSeq	NP_180788.2. NM_128788.2.
UniGene	At.38097. At.38099.
3D structure databases
HSSP	HSSP built from PDB template 1UKO based on UniProtKB P10538.
ProteinModelPortal	Q8L762.
SMR	Q8L762. Positions 73-558.
ModBase	Search...
Protein family/group databases
CAZy	GH14. Glycoside Hydrolase Family 14.
Proteomic databases
PRIDE	Q8L762.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G32290.1; AT2G32290.1; AT2G32290.
GeneID	817789.
GenomeReviews	Gene locus AT2G32290 in contig CT485783_GR.
KEGG	ath:AT2G32290.
Organism-specific databases
TAIR	At2g32290.
Phylogenomic databases
eggNOG	NOG77898.
GeneTree	EPGT00070000030150.
HOGENOM	HBG747772.
InParanoid	Q8L762.
OMA	RENMEDF.
PhylomeDB	Q8L762.
ProtClustDB	PLN02801.
Gene expression databases
Genevestigator	Q8L762.
Family and domain databases
InterPro	IPR001554. Glyco_hydro_14. IPR018238. Glyco_hydro_14_CS. IPR001371. Glyco_hydro_14B_pln. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF01373. Glyco_hydro_14. 1 hit. [Graphical view]
PRINTS	PR00750. BETAAMYLASE. PR00842. GLHYDLASE14B.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00506. BETA_AMYLASE_1. False negative. PS00679. BETA_AMYLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BAM6_ARATH

Accession

Primary (citable) accession number: Q8L762
Secondary accession number(s): Q9ZV58

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program