Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8L743

- G6PD3_ARATH

UniProt

Q8L743 - G6PD3_ARATH

Protein

Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic

Gene

At1g24280

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (25 Nov 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

    Catalytic activityi

    D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

    Enzyme regulationi

    Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei153 – 1531NADPBy similarity
    Binding sitei256 – 2561NADP; via carbonyl oxygenBy similarity
    Binding sitei256 – 2561SubstrateBy similarity
    Binding sitei324 – 3241SubstrateBy similarity
    Binding sitei343 – 3431SubstrateBy similarity
    Active sitei348 – 3481Proton acceptorBy similarity
    Binding sitei444 – 4441SubstrateBy similarity
    Binding sitei449 – 4491SubstrateBy similarity
    Binding sitei485 – 4851SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi119 – 1268NADPBy similarity

    GO - Molecular functioni

    1. glucose-6-phosphate dehydrogenase activity Source: TAIR
    2. NADP binding Source: InterPro
    3. protein binding Source: TAIR

    GO - Biological processi

    1. glucose metabolic process Source: TAIR
    2. pentose-phosphate shunt, oxidative branch Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00115; UER00408.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic (EC:1.1.1.49)
    Short name:
    G6PD3
    Short name:
    G6PDH3
    Gene namesi
    Ordered Locus Names:At1g24280
    ORF Names:F3I6.22
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G24280.

    Subcellular locationi

    Plastidchloroplast By similarity

    GO - Cellular componenti

    1. chloroplast Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5757ChloroplastSequence AnalysisAdd
    BLAST
    Chaini58 – 599542Glucose-6-phosphate 1-dehydrogenase 3, chloroplasticPRO_0000010437Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi171 ↔ 179Redox modulationBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ8L743.
    PRIDEiQ8L743.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8L743.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi24282. 1 interaction.
    IntActiQ8L743. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L743.
    SMRiQ8L743. Positions 113-578.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni286 – 2905Substrate bindingBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi78 – 814Poly-Ser
    Compositional biasi422 – 4254Poly-Ala

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0364.
    HOGENOMiHOG000046192.
    InParanoidiQ8L743.
    KOiK00036.
    OMAiYEIQEAN.
    PhylomeDBiQ8L743.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8L743-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSLSCPTYR SRTSSSSPFL SNHHHSSLIN VVDPRRSLSF HYASPQGLNL    50
    AELCVVRSQR RSVQSSVVVQ DGSVATESSS SEEAKDVGVL TIPSLEADKV 100
    VAESDGGEQL STVSITVVGA SGDLAKKKIF PALFALYYEG CLPEHFTIFG 150
    YARSKMTDAE LRVMVSKTLT CRIDKRANCG EKMEEFLKRC FYHSGQYDSQ 200
    EHFVALDEKL KEHEGGRLSN RLFYLSIPPN IFVDAVKCAS SSASSVNGWT 250
    RVIVEKPFGR DSKTSAALTK SLKQYLEEDQ IFRIDHYLGK ELVENLSVLR 300
    FSNLIFEPLW SRQYIRNVQF IFSEDFGTEG RGGYFDNYGI IRDIMQNHLL 350
    QILALFAMET PVSLDAEDIR NEKVKVLRSM RPIKLEDVVI GQYKSHSIGG 400
    VTYPSYTDDK TVPKGSLTPT FAAAALFIDN ARWDGVPFLM KAGKALNTRS 450
    AEIRVQFRHV PGNLYNRNSG TDRDQTTNEL VIRVQPDEAI YLKINNKVPG 500
    LGMRLDQSNL NLLYSARYSK EIPDAYERLL LDAIEGERRL FIRSDELDAA 550
    WALFTPLLKE IEEKKTTPEF YPYGSRGPVG AHYLAAKHKV QWGDLSLDQ 599
    Length:599
    Mass (Da):67,358
    Last modified:November 25, 2002 - v2
    Checksum:iAC0964649C5DB49F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti79 – 791S → N in AAM98087. (PubMed:14593172)Curated
    Sequence conflicti79 – 791S → N in AAO23597. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC002396 Genomic DNA. Translation: AAC00588.1.
    CP002684 Genomic DNA. Translation: AEE30511.1.
    AY139768 mRNA. Translation: AAM98087.1.
    BT003032 mRNA. Translation: AAO23597.1.
    PIRiT00659.
    RefSeqiNP_173838.1. NM_102274.2.
    UniGeneiAt.41453.
    At.63976.

    Genome annotation databases

    EnsemblPlantsiAT1G24280.1; AT1G24280.1; AT1G24280.
    GeneIDi839044.
    KEGGiath:AT1G24280.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC002396 Genomic DNA. Translation: AAC00588.1 .
    CP002684 Genomic DNA. Translation: AEE30511.1 .
    AY139768 mRNA. Translation: AAM98087.1 .
    BT003032 mRNA. Translation: AAO23597.1 .
    PIRi T00659.
    RefSeqi NP_173838.1. NM_102274.2.
    UniGenei At.41453.
    At.63976.

    3D structure databases

    ProteinModelPortali Q8L743.
    SMRi Q8L743. Positions 113-578.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 24282. 1 interaction.
    IntActi Q8L743. 1 interaction.

    Proteomic databases

    PaxDbi Q8L743.
    PRIDEi Q8L743.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G24280.1 ; AT1G24280.1 ; AT1G24280 .
    GeneIDi 839044.
    KEGGi ath:AT1G24280.

    Organism-specific databases

    TAIRi AT1G24280.

    Phylogenomic databases

    eggNOGi COG0364.
    HOGENOMi HOG000046192.
    InParanoidi Q8L743.
    KOi K00036.
    OMAi YEIQEAN.
    PhylomeDBi Q8L743.

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00408 .

    Gene expression databases

    Genevestigatori Q8L743.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00966. G6PD.
    InterProi IPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23429. PTHR23429. 1 hit.
    Pfami PF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00079. G6PDHDRGNASE.
    TIGRFAMsi TIGR00871. zwf. 1 hit.
    PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiG6PD3_ARATH
    AccessioniPrimary (citable) accession number: Q8L743
    Secondary accession number(s): O48695, Q53YG8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2002
    Last sequence update: November 25, 2002
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3