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Q8L743 (G6PD3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic
Short name=G6PD3
Short name=G6PDH3
EC=1.1.1.49
Gene names
Ordered Locus Names:At1g24280
ORF Names:F3I6.22
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length599 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulation

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastid › chloroplast By similarity.

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandNADP
   Molecular functionOxidoreductase
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processpentose-phosphate shunt, oxidative branch

Inferred from direct assay. Source: TAIR

   Cellular componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNADP binding

Inferred from electronic annotation. Source: InterPro

glucose-6-phosphate dehydrogenase activity

Inferred from direct assay. Source: TAIR

protein binding

Inferred from physical interaction. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5757Chloroplast Potential
Chain58 – 599542Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic
PRO_0000010437

Regions

Compositional bias78 – 814Poly-Ser
Compositional bias422 – 4254Poly-Ala

Sites

Active site3481Proton acceptor By similarity
Binding site1211NADP By similarity
Binding site1531NADP By similarity
Binding site2861Substrate By similarity
Binding site2901Substrate By similarity

Amino acid modifications

Disulfide bond171 ↔ 179Redox modulation By similarity

Experimental info

Sequence conflict791S → N in AAM98087. Ref.3
Sequence conflict791S → N in AAO23597. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8L743 [UniParc].

Last modified November 25, 2002. Version 2.
Checksum: AC0964649C5DB49F

FASTA59967,358
        10         20         30         40         50         60 
MSSLSCPTYR SRTSSSSPFL SNHHHSSLIN VVDPRRSLSF HYASPQGLNL AELCVVRSQR 

        70         80         90        100        110        120 
RSVQSSVVVQ DGSVATESSS SEEAKDVGVL TIPSLEADKV VAESDGGEQL STVSITVVGA 

       130        140        150        160        170        180 
SGDLAKKKIF PALFALYYEG CLPEHFTIFG YARSKMTDAE LRVMVSKTLT CRIDKRANCG 

       190        200        210        220        230        240 
EKMEEFLKRC FYHSGQYDSQ EHFVALDEKL KEHEGGRLSN RLFYLSIPPN IFVDAVKCAS 

       250        260        270        280        290        300 
SSASSVNGWT RVIVEKPFGR DSKTSAALTK SLKQYLEEDQ IFRIDHYLGK ELVENLSVLR 

       310        320        330        340        350        360 
FSNLIFEPLW SRQYIRNVQF IFSEDFGTEG RGGYFDNYGI IRDIMQNHLL QILALFAMET 

       370        380        390        400        410        420 
PVSLDAEDIR NEKVKVLRSM RPIKLEDVVI GQYKSHSIGG VTYPSYTDDK TVPKGSLTPT 

       430        440        450        460        470        480 
FAAAALFIDN ARWDGVPFLM KAGKALNTRS AEIRVQFRHV PGNLYNRNSG TDRDQTTNEL 

       490        500        510        520        530        540 
VIRVQPDEAI YLKINNKVPG LGMRLDQSNL NLLYSARYSK EIPDAYERLL LDAIEGERRL 

       550        560        570        580        590 
FIRSDELDAA WALFTPLLKE IEEKKTTPEF YPYGSRGPVG AHYLAAKHKV QWGDLSLDQ

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC002396 Genomic DNA. Translation: AAC00588.1.
CP002684 Genomic DNA. Translation: AEE30511.1.
AY139768 mRNA. Translation: AAM98087.1.
BT003032 mRNA. Translation: AAO23597.1.
IPIIPI00525584.
PIRT00659.
RefSeqNP_173838.1. NM_102274.2.
UniGeneAt.41453.
At.63976.

3D structure databases

ProteinModelPortalQ8L743.
SMRQ8L743. Positions 110-594.
ModBaseSearch...

Protein-protein interaction databases

IntActQ8L743. 2 interactions.
STRINGQ8L743.

Proteomic databases

PRIDEQ8L743.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G24280.1; AT1G24280.1; AT1G24280.
GeneID839044.
GenomeReviewsGene locus AT1G24280 in contig CT485782_GR.
KEGGath:AT1G24280.
NMPDRfig|3702.1.peg.2822.

Organism-specific databases

TAIRAt1g24280.

Phylogenomic databases

eggNOGKOG0563.
GeneTreeEPGT00070000029343.
HOGENOMHBG322449.
InParanoidQ8L743.
OMAMSPESET.
PhylomeDBQ8L743.
ProtClustDBPLN02333.

Gene expression databases

ArrayExpressQ8L743.
GenevestigatorQ8L743.
GermOnlineAT1G24280. Arabidopsis thaliana.

Family and domain databases

InterProIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00036.
PANTHERPTHR23429. G6PDH. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. Zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG6PD3_ARATH
AccessionPrimary (citable) accession number: Q8L743
Secondary accession number(s): O48695, Q53YG8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: December 14, 2011
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents