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Protein

Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic

Gene

At1g24280

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulationi

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation (By similarity).By similarity

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic (At1g24280), Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic (APG1), Glucose-6-phosphate 1-dehydrogenase (At3g27300), Glucose-6-phosphate 1-dehydrogenase (AXX17_At3g29760), Glucose-6-phosphate 1-dehydrogenase (G6PD4), Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic (At5g13110), Glucose-6-phosphate 1-dehydrogenase (AXX17_At1g25530), Glucose-6-phosphate 1-dehydrogenase (At5g35790), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g33080), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g33080), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g12520), Glucose-6-phosphate 1-dehydrogenase (G6PD5), Glucose-6-phosphate 1-dehydrogenase (G6PD5), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2 (ACG12), Glucose-6-phosphate 1-dehydrogenase (At1g09420), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g38480), Glucose-6-phosphate 1-dehydrogenase (AXX17_At1g09330), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 (ACG9), Glucose-6-phosphate 1-dehydrogenase (At5g40760), Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic (At1g09420)
  2. Probable 6-phosphogluconolactonase 5, chloroplastic (EMB2024), Probable 6-phosphogluconolactonase 3 (At5g24420), Probable 6-phosphogluconolactonase 4 (At5g24410), Probable 6-phosphogluconolactonase 1 (At1g13700), Probable 6-phosphogluconolactonase 2 (At3g49360)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At5g39410), 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At3g01580), 6-phosphogluconate dehydrogenase, decarboxylating 2, chloroplastic (At5g41670), 6-phosphogluconate dehydrogenase, decarboxylating 3 (At3g02360), 6-phosphogluconate dehydrogenase, decarboxylating 1, chloroplastic (At1g64190), 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At1g57660)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei153NADPBy similarity1
Binding sitei256NADP; via carbonyl oxygenBy similarity1
Binding sitei256SubstrateBy similarity1
Binding sitei324SubstrateBy similarity1
Binding sitei343SubstrateBy similarity1
Active sitei348Proton acceptorBy similarity1
Binding sitei444SubstrateBy similarity1
Binding sitei449SubstrateBy similarity1
Binding sitei485SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi119 – 126NADPBy similarity8

GO - Molecular functioni

  • glucose-6-phosphate dehydrogenase activity Source: TAIR
  • NADP binding Source: InterPro

GO - Biological processi

  • glucose metabolic process Source: TAIR
  • pentose-phosphate shunt, oxidative branch Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
LigandNADP

Enzyme and pathway databases

BRENDAi1.1.1.49 399
UniPathwayiUPA00115; UER00408

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic (EC:1.1.1.49)
Short name:
G6PD3
Short name:
G6PDH3
Gene namesi
Ordered Locus Names:At1g24280
ORF Names:F3I6.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G24280
TAIRilocus:2032412 AT1G24280

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 66ChloroplastCombined sourcesAdd BLAST66
ChainiPRO_000001043767 – 599Glucose-6-phosphate 1-dehydrogenase 3, chloroplasticAdd BLAST533

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67N-acetylvalineCombined sources1
Disulfide bondi171 ↔ 179Redox modulationBy similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PaxDbiQ8L743
PRIDEiQ8L743

PTM databases

iPTMnetiQ8L743

Expressioni

Gene expression databases

ExpressionAtlasiQ8L743 baseline and differential
GenevisibleiQ8L743 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi24282, 2 interactors
IntActiQ8L743, 1 interactor
STRINGi3702.AT1G24280.1

Structurei

3D structure databases

ProteinModelPortaliQ8L743
SMRiQ8L743
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni286 – 290Substrate bindingBy similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi78 – 81Poly-Ser4
Compositional biasi422 – 425Poly-Ala4

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0563 Eukaryota
COG0364 LUCA
HOGENOMiHOG000046192
InParanoidiQ8L743
KOiK00036
OMAiPEFYPYG
OrthoDBiEOG093605BG
PhylomeDBiQ8L743

Family and domain databases

HAMAPiMF_00966 G6PD, 1 hit
InterProiView protein in InterPro
IPR001282 G6P_DH
IPR019796 G6P_DH_AS
IPR022675 G6P_DH_C
IPR022674 G6P_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23429 PTHR23429, 1 hit
PfamiView protein in Pfam
PF02781 G6PD_C, 1 hit
PF00479 G6PD_N, 1 hit
PRINTSiPR00079 G6PDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00871 zwf, 1 hit
PROSITEiView protein in PROSITE
PS00069 G6P_DEHYDROGENASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L743-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLSCPTYR SRTSSSSPFL SNHHHSSLIN VVDPRRSLSF HYASPQGLNL
60 70 80 90 100
AELCVVRSQR RSVQSSVVVQ DGSVATESSS SEEAKDVGVL TIPSLEADKV
110 120 130 140 150
VAESDGGEQL STVSITVVGA SGDLAKKKIF PALFALYYEG CLPEHFTIFG
160 170 180 190 200
YARSKMTDAE LRVMVSKTLT CRIDKRANCG EKMEEFLKRC FYHSGQYDSQ
210 220 230 240 250
EHFVALDEKL KEHEGGRLSN RLFYLSIPPN IFVDAVKCAS SSASSVNGWT
260 270 280 290 300
RVIVEKPFGR DSKTSAALTK SLKQYLEEDQ IFRIDHYLGK ELVENLSVLR
310 320 330 340 350
FSNLIFEPLW SRQYIRNVQF IFSEDFGTEG RGGYFDNYGI IRDIMQNHLL
360 370 380 390 400
QILALFAMET PVSLDAEDIR NEKVKVLRSM RPIKLEDVVI GQYKSHSIGG
410 420 430 440 450
VTYPSYTDDK TVPKGSLTPT FAAAALFIDN ARWDGVPFLM KAGKALNTRS
460 470 480 490 500
AEIRVQFRHV PGNLYNRNSG TDRDQTTNEL VIRVQPDEAI YLKINNKVPG
510 520 530 540 550
LGMRLDQSNL NLLYSARYSK EIPDAYERLL LDAIEGERRL FIRSDELDAA
560 570 580 590
WALFTPLLKE IEEKKTTPEF YPYGSRGPVG AHYLAAKHKV QWGDLSLDQ
Length:599
Mass (Da):67,358
Last modified:November 25, 2002 - v2
Checksum:iAC0964649C5DB49F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79S → N in AAM98087 (PubMed:14593172).Curated1
Sequence conflicti79S → N in AAO23597 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002396 Genomic DNA Translation: AAC00588.1
CP002684 Genomic DNA Translation: AEE30511.1
AY139768 mRNA Translation: AAM98087.1
BT003032 mRNA Translation: AAO23597.1
PIRiT00659
RefSeqiNP_173838.1, NM_102274.3
UniGeneiAt.41453
At.63976

Genome annotation databases

EnsemblPlantsiAT1G24280.1; AT1G24280.1; AT1G24280
GeneIDi839044
GrameneiAT1G24280.1; AT1G24280.1; AT1G24280
KEGGiath:AT1G24280

Similar proteinsi

Entry informationi

Entry nameiG6PD3_ARATH
AccessioniPrimary (citable) accession number: Q8L743
Secondary accession number(s): O48695, Q53YG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: April 25, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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