Reviewed, UniProtKB/Swiss-Prot Q8L743 (G6PD3_ARATH)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic
      Short name=G6PDH3
      Short name=G6PD3
    EC=1.1.1.49

Gene names

Ordered Locus Names:	At1g24280
ORF Names:	F3I6.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.
Catalytic activity	D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH.
Enzyme regulation	Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast By similarity.
Miscellaneous	There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt, oxidative branch Inferred from direct assay. Source: TAIR
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucose-6-phosphate dehydrogenase activity Inferred from direct assay. Source: TAIR protein binding Inferred from physical interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 57	57	Chloroplast Potential
	Chain	58 – 599	542	Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic		PRO_0000010437
Regions
	Compositional bias	78 – 81	4	Poly-Ser
	Compositional bias	422 – 425	4	Poly-Ala
Sites
	Active site	348	1	Proton acceptor By similarity
	Binding site	121	1	NADP By similarity
	Binding site	153	1	NADP By similarity
	Binding site	286	1	Substrate By similarity
	Binding site	290	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	171 ↔ 179		Redox modulation By similarity
Experimental info
	Sequence conflict	79	1	S → N in AAM98087. Ref.2
	Sequence conflict	79	1	S → N in AAO23597. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8L743-1 [UniParc]. Last modified November 25, 2002. Version 2. Checksum: AC0964649C5DB49F	FASTA	599	67,358
	10 20 30 40 50 60 MSSLSCPTYR SRTSSSSPFL SNHHHSSLIN VVDPRRSLSF HYASPQGLNL AELCVVRSQR 70 80 90 100 110 120 RSVQSSVVVQ DGSVATESSS SEEAKDVGVL TIPSLEADKV VAESDGGEQL STVSITVVGA 130 140 150 160 170 180 SGDLAKKKIF PALFALYYEG CLPEHFTIFG YARSKMTDAE LRVMVSKTLT CRIDKRANCG 190 200 210 220 230 240 EKMEEFLKRC FYHSGQYDSQ EHFVALDEKL KEHEGGRLSN RLFYLSIPPN IFVDAVKCAS 250 260 270 280 290 300 SSASSVNGWT RVIVEKPFGR DSKTSAALTK SLKQYLEEDQ IFRIDHYLGK ELVENLSVLR 310 320 330 340 350 360 FSNLIFEPLW SRQYIRNVQF IFSEDFGTEG RGGYFDNYGI IRDIMQNHLL QILALFAMET 370 380 390 400 410 420 PVSLDAEDIR NEKVKVLRSM RPIKLEDVVI GQYKSHSIGG VTYPSYTDDK TVPKGSLTPT 430 440 450 460 470 480 FAAAALFIDN ARWDGVPFLM KAGKALNTRS AEIRVQFRHV PGNLYNRNSG TDRDQTTNEL 490 500 510 520 530 540 VIRVQPDEAI YLKINNKVPG LGMRLDQSNL NLLYSARYSK EIPDAYERLL LDAIEGERRL 550 560 570 580 590 FIRSDELDAA WALFTPLLKE IEEKKTTPEF YPYGSRGPVG AHYLAAKHKV QWGDLSLDQ

References

[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002396 Genomic DNA. Translation: AAC00588.1. AY139768 mRNA. Translation: AAM98087.1. BT003032 mRNA. Translation: AAO23597.1.
IPI	IPI00525584.
PIR	T00659.
RefSeq	NP_173838.1.
UniGene	At.41453 At.63976 Rra.21952
3D structure databases
SMR	Q8L743. Positions 109-594.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8L743.
Proteomic databases
PRIDE	Q8L743.
Genome annotation databases
GeneID	839044.
GenomeReviews	Gene locus AT1G24280 in contig CT485782_GR.
KEGG	ath:AT1G24280.
NMPDR	fig|3702.1.peg.2822.
Organism-specific databases
TAIR	At1g24280.
Phylogenomic databases
eggNOG	KOG0563.
HOGENOM	HBG322449.
InParanoid	Q8L743.
OMA	KEHEGGR.
PhylomeDB	Q8L743.
Enzyme and pathway databases
BRENDA	1.1.1.49. 302.
Gene expression databases
ArrayExpress	Q8L743.
Genevestigator	Q8L743.
GermOnline	AT1G24280. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DH. IPR019796. Glc-6-P_DH_AS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PRINTS	PR00079. G6PDHDRGNASE.
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PD3_ARATH

Accession

Primary (citable) accession number: Q8L743
Secondary accession number(s): O48695, Q53YG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	November 25, 2002
Last modified:	February 9, 2010
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)