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Q8L743 (G6PD3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic

Short name=G6PD3
Short name=G6PDH3
EC=1.1.1.49
Gene names
Ordered Locus Names:At1g24280
ORF Names:F3I6.22
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length599 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division. HAMAP-Rule MF_00966

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. HAMAP-Rule MF_00966

Enzyme regulation

Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation By similarity. HAMAP-Rule MF_00966

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. HAMAP-Rule MF_00966

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00966

Subcellular location

Plastidchloroplast By similarity HAMAP-Rule MF_00966.

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5757Chloroplast Potential
Chain58 – 599542Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic HAMAP-Rule MF_00966
PRO_0000010437

Regions

Nucleotide binding119 – 1268NADP By similarity
Region286 – 2905Substrate binding By similarity
Compositional bias78 – 814Poly-Ser HAMAP-Rule MF_00966
Compositional bias422 – 4254Poly-Ala HAMAP-Rule MF_00966

Sites

Active site3481Proton acceptor By similarity
Binding site1531NADP By similarity
Binding site2561NADP; via carbonyl oxygen By similarity
Binding site2561Substrate By similarity
Binding site3241Substrate By similarity
Binding site3431Substrate By similarity
Binding site4441Substrate By similarity
Binding site4491Substrate By similarity
Binding site4851Substrate By similarity

Amino acid modifications

Disulfide bond171 ↔ 179Redox modulation By similarity

Experimental info

Sequence conflict791S → N in AAM98087. Ref.3
Sequence conflict791S → N in AAO23597. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8L743 [UniParc].

Last modified November 25, 2002. Version 2.
Checksum: AC0964649C5DB49F

FASTA59967,358
        10         20         30         40         50         60 
MSSLSCPTYR SRTSSSSPFL SNHHHSSLIN VVDPRRSLSF HYASPQGLNL AELCVVRSQR 

        70         80         90        100        110        120 
RSVQSSVVVQ DGSVATESSS SEEAKDVGVL TIPSLEADKV VAESDGGEQL STVSITVVGA 

       130        140        150        160        170        180 
SGDLAKKKIF PALFALYYEG CLPEHFTIFG YARSKMTDAE LRVMVSKTLT CRIDKRANCG 

       190        200        210        220        230        240 
EKMEEFLKRC FYHSGQYDSQ EHFVALDEKL KEHEGGRLSN RLFYLSIPPN IFVDAVKCAS 

       250        260        270        280        290        300 
SSASSVNGWT RVIVEKPFGR DSKTSAALTK SLKQYLEEDQ IFRIDHYLGK ELVENLSVLR 

       310        320        330        340        350        360 
FSNLIFEPLW SRQYIRNVQF IFSEDFGTEG RGGYFDNYGI IRDIMQNHLL QILALFAMET 

       370        380        390        400        410        420 
PVSLDAEDIR NEKVKVLRSM RPIKLEDVVI GQYKSHSIGG VTYPSYTDDK TVPKGSLTPT 

       430        440        450        460        470        480 
FAAAALFIDN ARWDGVPFLM KAGKALNTRS AEIRVQFRHV PGNLYNRNSG TDRDQTTNEL 

       490        500        510        520        530        540 
VIRVQPDEAI YLKINNKVPG LGMRLDQSNL NLLYSARYSK EIPDAYERLL LDAIEGERRL 

       550        560        570        580        590 
FIRSDELDAA WALFTPLLKE IEEKKTTPEF YPYGSRGPVG AHYLAAKHKV QWGDLSLDQ 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC002396 Genomic DNA. Translation: AAC00588.1.
CP002684 Genomic DNA. Translation: AEE30511.1.
AY139768 mRNA. Translation: AAM98087.1.
BT003032 mRNA. Translation: AAO23597.1.
PIRT00659.
RefSeqNP_173838.1. NM_102274.2.
UniGeneAt.41453.
At.63976.

3D structure databases

ProteinModelPortalQ8L743.
SMRQ8L743. Positions 113-578.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid24282. 1 interaction.
IntActQ8L743. 1 interaction.

Proteomic databases

PaxDbQ8L743.
PRIDEQ8L743.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G24280.1; AT1G24280.1; AT1G24280.
GeneID839044.
KEGGath:AT1G24280.

Organism-specific databases

TAIRAT1G24280.

Phylogenomic databases

eggNOGCOG0364.
HOGENOMHOG000046192.
InParanoidQ8L743.
KOK00036.
OMAYEIQEAN.
PhylomeDBQ8L743.

Enzyme and pathway databases

UniPathwayUPA00115; UER00408.

Gene expression databases

GenevestigatorQ8L743.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00966. G6PD.
InterProIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG6PD3_ARATH
AccessionPrimary (citable) accession number: Q8L743
Secondary accession number(s): O48695, Q53YG8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: June 11, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names