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Protein

Isoamylase 2, chloroplastic

Gene

ISA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the trimming of pre-amylopectin chains. Accelerates the crystallization of nascent amylopectin molecules during starch synthesis. ISA1 and ISA2 work exclusively together as a multimeric holoenzyme. ISA1-ISA2 removes preferentially branches that are very close to other branches.3 Publications

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

  • isoamylase activity Source: TAIR

GO - Biological processi

  • amylopectin biosynthetic process Source: TAIR
  • starch biosynthetic process Source: UniProtKB-UniPathway
  • starch catabolic process Source: EnsemblPlants

Keywordsi

Biological processCarbohydrate metabolism, Starch biosynthesis

Enzyme and pathway databases

BioCyciARA:AT1G03310-MONOMER.
ARA:GQT-2133-MONOMER.
MetaCyc:AT1G03310-MONOMER.
BRENDAi3.2.1.68. 399.
UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoamylase 2, chloroplastic
Short name:
AtISA2
Alternative name(s):
Protein DEBRANCHING ENZYME 1
Gene namesi
Name:ISA2
Synonyms:DBE1
Ordered Locus Names:At1g03310
ORF Names:F15K9.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G03310.
TAIRilocus:2014500. AT1G03310.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Strong reduction of the starch level in leaves, but 50-fold increase of water-soluble polysaccharides. No alteration of the amylase-to-amylopectin ratio.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 70ChloroplastCuratedAdd BLAST70
ChainiPRO_000037952871 – 882Isoamylase 2, chloroplasticAdd BLAST812

Proteomic databases

PaxDbiQ8L735.

Expressioni

Gene expression databases

ExpressionAtlasiQ8L735. baseline and differential.
GenevisibleiQ8L735. AT.

Interactioni

Subunit structurei

Associates with ISA1 to form the heteromultimeric complex Iso1 required for amylopectin synthesis.

Protein-protein interaction databases

STRINGi3702.AT1G03310.1.

Structurei

3D structure databases

ProteinModelPortaliQ8L735.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi788 – 793Poly-Glu6

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IN7N. Eukaryota.
COG1523. LUCA.
HOGENOMiHOG000239197.
InParanoidiQ8L735.
KOiK01214.
OMAiWHVSLAS.
OrthoDBiEOG093602VB.
PhylomeDBiQ8L735.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
InterProiView protein in InterPro
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L735-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAWSPSVGI GSCCLNNGIT RTWKFPSARL FTGRKNKIKL GSETLMFTRK
60 70 80 90 100
RFMGDLVTSA LQSYQFSKIC ASKTSIELRE ALSSRRAEAD DLKKVTSYSF
110 120 130 140 150
RTKAGALVKV KVEKKREKYS ILVYVSSLEL SGDDKSRLVM VWGVYRSDSS
160 170 180 190 200
CFLPLDFENS SQDSQTHTTE TTFVKSSLSE LMLGLEFDGK ESPFYLSFHL
210 220 230 240 250
KLVSGRDPDG QEMLTHRDTD FCIPVGFTAG HPLPLGLSSG PDDDSWNFSF
260 270 280 290 300
FSRSSTNVVL CLYDDSTTDK PALELDLDPY VNRTGDVWHA SVDNTWDFVR
310 320 330 340 350
YGYRCKETAH SKEDVDVEGE PIVLDPYATV VGKSVSQKYL GSLSKSPSFD
360 370 380 390 400
WGEDVSPNIP LEKLLVYRLN VKGFTQHRSS KLPSNVAGTF SGVAEKVSHL
410 420 430 440 450
KTLGTNAVLL EPIFSFSEQK GPYFPFHFFS PMDIYGPSNS LESAVNSMKV
460 470 480 490 500
MVKKLHSEGI EVLLEVVFTH TADSGALRGI DDSSYYYKGR ANDLDSKSYL
510 520 530 540 550
NCNYPVVQQL VLESLRYWVT EFHVDGFCFI NASSLLRGVH GEQLSRPPLV
560 570 580 590 600
EAIAFDPLLA ETKLIADCWD PLEMMPKEVR FPHWKRWAEL NTRYCRNVRN
610 620 630 640 650
FLRGRGVLSD LATRICGSGD VFTDGRGPAF SFNYISRNSG LSLVDIVSFS
660 670 680 690 700
GPELASELSW NCGEEGATNK SAVLQRRLKQ IRNFLFIQYI SLGVPVLNMG
710 720 730 740 750
DECGISTRGS PLLESRKPFD WNLLASAFGT QITQFISFMT SVRARRSDVF
760 770 780 790 800
QRRDFLKPEN IVWYANDQTT PKWEDPASKF LALEIKSESE EEETASLAEP
810 820 830 840 850
NEPKSNDLFI GFNASDHPES VVLPSLPDGS KWRRLVDTAL PFPGFFSVEG
860 870 880
ETVVAEEPLQ QLVVYEMKPY SCTLFETINT TA
Length:882
Mass (Da):98,884
Last modified:July 7, 2009 - v2
Checksum:i277940176F03B06D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211Q → R in AAM98123 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005278 Genomic DNA. Translation: AAC72113.1.
CP002684 Genomic DNA. Translation: AEE27557.1.
CP002684 Genomic DNA. Translation: AEE27558.1.
AY139980 mRNA. Translation: AAM98123.1.
PIRiF86164.
RefSeqiNP_171830.1. NM_100213.4.
NP_973751.1. NM_202022.3.
UniGeneiAt.24041.

Genome annotation databases

EnsemblPlantsiAT1G03310.1; AT1G03310.1; AT1G03310.
AT1G03310.2; AT1G03310.2; AT1G03310.
GeneIDi839531.
GrameneiAT1G03310.1; AT1G03310.1; AT1G03310.
AT1G03310.2; AT1G03310.2; AT1G03310.
KEGGiath:AT1G03310.

Similar proteinsi

Entry informationi

Entry nameiISOA2_ARATH
AccessioniPrimary (citable) accession number: Q8L735
Secondary accession number(s): Q9ZVT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: July 7, 2009
Last modified: March 28, 2018
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Amino acids thought to be required for catalysis are not conserved in ISA2, suggesting that it may not be an active debranching enzyme and acts via its interaction with ISA1.Curated

Keywords - Technical termi

Complete proteome, Reference proteome