Q8L727 (GSTT2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase T2
Short name=AtGSTT2
EC=2.5.1.18

Alternative name(s):
GST class-theta member 2
Glutathione S-transferase 10B

Gene names

Name:	GSTT2
Synonyms:	GST10B
Ordered Locus Names:	At5g41240
ORF Names:	K1O13.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Peroxisome Ref.6.
Sequence similarities	Belongs to the GST superfamily. Theta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain. Contains 1 Myb-like domain.
Sequence caution	The sequence BAB11099.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes. The sequence BAB11100.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes. The sequence CAA10662.1 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
Biological process	Detoxification
Cellular component	Peroxisome
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to toxin Inferred from electronic annotation. Source: UniProtKB-KW toxin catabolic process Traceable author statement Ref.5. Source: TAIR
Cellular_component	cytoplasm Non-traceable author statement Ref.5. Source: TAIR peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Glutathione S-transferase T2		PRO_0000413575
Regions
	Domain	1 – 82	82	GST N-terminal
	Domain	89 – 226	138	GST C-terminal
	Domain	265 – 338	74	Myb-like
	Region	11 – 12	2	Glutathione binding By similarity
	Region	40 – 41	2	Glutathione binding By similarity
	Region	53 – 54	2	Glutathione binding By similarity
	Region	66 – 67	2	Glutathione binding By similarity
Experimental info
	Sequence conflict	1	1	M → MM in CAA10662. Ref.4
	Sequence conflict	9	1	R → K in CAA10662. Ref.4
	Sequence conflict	125	1	G → S in CAA10662. Ref.4
	Sequence conflict	244	1	I → M in CAA10662. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8L727 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D1F401C7FDA853D9	FASTA	591	67,689
	10 20 30 40 50 60 MKLKVYADRM SQPSRAVLIF CKVNEIQFDE ILISLGKRQQ LSPEFKEINP MGKVPAIVDG 70 80 90 100 110 120 RLKLFESHAI LIYLSSAYAS VVDHWYPNDL SKRAKIHSVL DWHHTNLRPG ASGYVLNSVL 130 140 150 160 170 180 APALGLPLNP KAAAEAENIL TNSLSTLETF WLKGSAKFLL GGKQPSIADL SLVCELMQLQ 190 200 210 220 230 240 VLDDKDRLRL LSPHKKVEQW IESTRKATMP HSDEVHEVLF RAKDRFQKQR EMATASKPGP 250 260 270 280 290 300 QSKIIQFSSI GGTSDGPNLV QDTTDRKARR RKWSPPDDVI LISAWLNTSK DRKVVVYDEQ 310 320 330 340 350 360 QAHTFWKRIG AHVSNSASLA NLPKREWNHC RQRWRKINDY VCKFVGCYDQ ALNQRASGQS 370 380 390 400 410 420 EDDVFQVAYQ LYYNNYMSNF KLEHAWRELR HNKKWCSTYT SENSKGGGSS KRTKLNGGGV 430 440 450 460 470 480 YSSSCNPESV PIALDGEEQV MDRPLGVKSS KQKEKKVATK TMLEEREADS RSRLENLWVL 490 500 510 520 530 540 DEEEQVMDLP LGVKSSKQKE RKVATKTMIE EREAANFRSR LGNLWLLKEK EEREADSRSR 550 560 570 580 590 LENLWALKEK DIEEQKKLTR MEVLKSLLGR RTGETSEKEE TLKNKLIDEM L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Identification and cloning of AtGST 10, members of a novel type of plant glutathione transferases." Dixon D.P., Cole D.J., Edwards R. Plant Gene Register PGR99-053 Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-245. Strain: cv. Columbia.
[5]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily." Dixon D.P., Hawkins T., Hussey P.J., Edwards R. J. Exp. Bot. 60:1207-1218(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB019225 Genomic DNA. Translation: BAB11099.1. Sequence problems. AB019225 Genomic DNA. Translation: BAB11100.1. Sequence problems. CP002688 Genomic DNA. Translation: AED94656.1. AY139996 mRNA. Translation: AAM98138.1. BT008361 mRNA. Translation: AAP37720.1. AJ132398 mRNA. Translation: CAA10662.1. Sequence problems.
IPI	IPI00524043.
PIR	T52580.
RefSeq	NP_198940.3. NM_123489.3.
UniGene	At.22919.
3D structure databases
HSSP	HSSP built from PDB template 1LJR based on UniProtKB P30712.
ProteinModelPortal	Q8L727.
SMR	Q8L727. Positions 3-224.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G41240.1-P.
Proteomic databases
PaxDb	Q8L727.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G41240.1; AT5G41240.1; AT5G41240.
GeneID	834125.
KEGG	ath:AT5G41240.
Organism-specific databases
TAIR	At5g41240.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000148624.
InParanoid	Q8L727.
PhylomeDB	Q8L727.
ProtClustDB	CLSN2686230.
Gene expression databases
ArrayExpress	Q8L727.
Genevestigator	Q8L727.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR017877. Myb-like_dom. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. PS50090. MYB_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTT2_ARATH

Accession

Primary (citable) accession number: Q8L727
Secondary accession number(s): Q9FHD9, Q9FHE0, Q9ZRR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program