Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8L727 (GSTT2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione S-transferase T2

Short name=AtGSTT2
EC=2.5.1.18
Alternative name(s):
GST class-theta member 2
Glutathione S-transferase 10B
Gene names
Name:GSTT2
Synonyms:GST10B
Ordered Locus Names:At5g41240/At5g41230
ORF Names:K1O13.3/K1O13.2
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length591 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides By similarity.

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Subcellular location

Peroxisome Ref.6.

Sequence similarities

Belongs to the GST superfamily. Theta family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Contains 1 Myb-like domain.

Sequence caution

The sequence BAB11099.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g41230 and At5g41240.

The sequence BAB11100.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g41230 and At5g41240.

The sequence CAA10662.1 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
   Biological processDetoxification
   Cellular componentPeroxisome
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processresponse to toxic substance

Inferred from electronic annotation. Source: UniProtKB-KW

toxin catabolic process

Traceable author statement Ref.5. Source: TAIR

   Cellular_componentcytoplasm

Non-traceable author statement Ref.5. Source: TAIR

peroxisome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglutathione transferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 591591Glutathione S-transferase T2
PRO_0000413575

Regions

Domain1 – 8282GST N-terminal
Domain89 – 226138GST C-terminal
Domain265 – 33874Myb-like
Region11 – 122Glutathione binding By similarity
Region40 – 412Glutathione binding By similarity
Region53 – 542Glutathione binding By similarity
Region66 – 672Glutathione binding By similarity

Experimental info

Sequence conflict11M → MM in CAA10662. Ref.4
Sequence conflict91R → K in CAA10662. Ref.4
Sequence conflict1251G → S in CAA10662. Ref.4
Sequence conflict2441I → M in CAA10662. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q8L727 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: D1F401C7FDA853D9

FASTA59167,689
        10         20         30         40         50         60 
MKLKVYADRM SQPSRAVLIF CKVNEIQFDE ILISLGKRQQ LSPEFKEINP MGKVPAIVDG 

        70         80         90        100        110        120 
RLKLFESHAI LIYLSSAYAS VVDHWYPNDL SKRAKIHSVL DWHHTNLRPG ASGYVLNSVL 

       130        140        150        160        170        180 
APALGLPLNP KAAAEAENIL TNSLSTLETF WLKGSAKFLL GGKQPSIADL SLVCELMQLQ 

       190        200        210        220        230        240 
VLDDKDRLRL LSPHKKVEQW IESTRKATMP HSDEVHEVLF RAKDRFQKQR EMATASKPGP 

       250        260        270        280        290        300 
QSKIIQFSSI GGTSDGPNLV QDTTDRKARR RKWSPPDDVI LISAWLNTSK DRKVVVYDEQ 

       310        320        330        340        350        360 
QAHTFWKRIG AHVSNSASLA NLPKREWNHC RQRWRKINDY VCKFVGCYDQ ALNQRASGQS 

       370        380        390        400        410        420 
EDDVFQVAYQ LYYNNYMSNF KLEHAWRELR HNKKWCSTYT SENSKGGGSS KRTKLNGGGV 

       430        440        450        460        470        480 
YSSSCNPESV PIALDGEEQV MDRPLGVKSS KQKEKKVATK TMLEEREADS RSRLENLWVL 

       490        500        510        520        530        540 
DEEEQVMDLP LGVKSSKQKE RKVATKTMIE EREAANFRSR LGNLWLLKEK EEREADSRSR 

       550        560        570        580        590 
LENLWALKEK DIEEQKKLTR MEVLKSLLGR RTGETSEKEE TLKNKLIDEM L 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Identification and cloning of AtGST 10, members of a novel type of plant glutathione transferases."
Dixon D.P., Cole D.J., Edwards R.
Plant Gene Register PGR99-053
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-245.
Strain: cv. Columbia.
[5]"Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
Wagner U., Edwards R., Dixon D.P., Mauch F.
Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily."
Dixon D.P., Hawkins T., Hussey P.J., Edwards R.
J. Exp. Bot. 60:1207-1218(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB019225 Genomic DNA. Translation: BAB11099.1. Sequence problems.
AB019225 Genomic DNA. Translation: BAB11100.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED94656.1.
AY139996 mRNA. Translation: AAM98138.1.
BT008361 mRNA. Translation: AAP37720.1.
AJ132398 mRNA. Translation: CAA10662.1. Sequence problems.
PIRT52580.
RefSeqNP_198940.3. NM_123489.3.
UniGeneAt.22919.

3D structure databases

ProteinModelPortalQ8L727.
SMRQ8L727. Positions 1-250.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G41240.1-P.

Proteomic databases

PaxDbQ8L727.
PRIDEQ8L727.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G41240.1; AT5G41240.1; AT5G41240.
GeneID834125.
KEGGath:AT5G41240.

Organism-specific databases

TAIRAT5G41240.

Phylogenomic databases

eggNOGCOG0625.
HOGENOMHOG000148624.
InParanoidQ8L727.
OMAVILISAW.
PhylomeDBQ8L727.

Enzyme and pathway databases

BioCycARA:AT5G41240-MONOMER.

Gene expression databases

GenevestigatorQ8L727.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017877. Myb-like_dom.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
PS50090. MYB_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSTT2_ARATH
AccessionPrimary (citable) accession number: Q8L727
Secondary accession number(s): Q9FHD9, Q9FHE0, Q9ZRR6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names