Q8L707 (IRX14_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-1,4-xylosyltransferase IRX14
EC=2.4.2.-

Alternative name(s):
Protein IRREGULAR XYLEM 14
Xylan xylosyltransferase IRX14

Gene names

Name:	IRX14
Ordered Locus Names:	At4g36890
ORF Names:	AP22.51, C7A10.470

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls. Probably involved in the elongation of glucuronoxylan xylosyl backbone. Ref.6 Ref.7 Ref.8
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable Ref.7.
Tissue specificity	Expressed in developing interfascicular fibers and xylem cells in stems and developing secondary xylem in roots. Ref.7
Disruption phenotype	Reduced xylan content in cell wall. Ref.6
Sequence similarities	Belongs to the glycosyltransferase 43 family.
Sequence caution	The sequence CAB16800.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB80355.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW fruit development Inferred from mutant phenotype Ref.6. Source: TAIR glucuronoxylan biosynthetic process Inferred from mutant phenotype Ref.6. Source: TAIR shoot development Inferred from mutant phenotype Ref.6. Source: TAIR xylem and phloem pattern formation Inferred from mutant phenotype Ref.6. Source: TAIR
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: InterPro xylosyltransferase activity Inferred from mutant phenotype Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Probable beta-1,4-xylosyltransferase IRX14		PRO_0000407565
Regions
	Topological domain	1 – 35	35	Cytoplasmic Potential
	Transmembrane	36 – 56	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	57 – 525	469	Lumenal Potential
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Potential
	Glycosylation	204	1	N-linked (GlcNAc...) Potential
	Glycosylation	326	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8L707 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 025BFCC9A2C1C348	FASTA	525	59,099
	10 20 30 40 50 60 MKLSALHQSY LNRRSNSFRS PTSLDSSVDG SGKSLIAVFW LILHCLCCLI SLVLGFRFSR 70 80 90 100 110 120 LVFFFLFSTS STNLYSLPFR PDLPVKHLDV HTIGRTLDPG ANGTTVVATA TKSSRVVVGR 130 140 150 160 170 180 HGIRIRPWPH PNPVEVMKAH QIIGRVQKEQ KMIFGMKSSK MVIAVTPTYV RTFQALHLTG 190 200 210 220 230 240 VMHSLMLVPY DLVWIVVEAG GATNETGLII AKSGLRTIHV GIDQRMPNTW EDRSKLEVFM 250 260 270 280 290 300 RLQALRVVRE EKLDGIVMFA DDSNMHSMEL FDEIQNVKWF GTVSVGILAH SGNAEEMVLS 310 320 330 340 350 360 MEKRKEMEKE EEEESSSLPV QGPACNSTDQ LIGWHIFNTL PYAGKSAVYI DDVAAVLPQK 370 380 390 400 410 420 LEWSGFVLNS RLLWEEAENK PEWVKDFGSL NENEGVESPL SLLKDPSMVE PLGSCGRQVL 430 440 450 460 470 480 LWWLRVEARA DSKFPPGWII DPPLEITVAA KRTPWPDVPP EPPTKKKDQM PLSQGNTVVV 490 500 510 520 IPKQQQHPTK IRKPKRKSKK SKHEPRPTDT TTQVYSSSSK HQERN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana." Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N. Nature 391:485-488(1998) [PubMed: 9461215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis." Brown D.M., Goubet F., Wong V.W., Goodacre R., Stephens E., Dupree P., Turner S.R. Plant J. 52:1154-1168(2007) [PubMed: 17944810] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"The Arabidopsis family GT43 glycosyltransferases form two functionally nonredundant groups essential for the elongation of glucuronoxylan backbone." Lee C., Teng Q., Huang W., Zhong R., Ye Z.H. Plant Physiol. 153:526-541(2010) [PubMed: 20335400] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]	"Analysis of the Arabidopsis IRX9/IRX9-L and IRX14/IRX14-L pairs of glycosyltransferase genes reveals critical contributions to biosynthesis of the hemicellulose glucuronoxylan." Wu A.M., Hoernblad E., Voxeur A., Gerber L., Rihouey C., Lerouge P., Marchant A. Plant Physiol. 153:542-554(2010) [PubMed: 20424005] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z99707 Genomic DNA. Translation: CAB16800.1. Sequence problems. AL161590 Genomic DNA. Translation: CAB80355.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE86714.1. AY140036 mRNA. Translation: AAM98177.1. BT014969 mRNA. Translation: AAT47820.1.
IPI	IPI00536391.
PIR	F85435.
RefSeq	NP_195407.2. NM_119853.3.
UniGene	At.31289. At.67343.
3D structure databases
HSSP	HSSP built from PDB template 1KWS based on UniProtKB O94766.
ProteinModelPortal	Q8L707.
SMR	Q8L707. Positions 161-429.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8L707.
Protein family/group databases
CAZy	GT43. Glycosyltransferase Family 43.
Proteomic databases
PRIDE	Q8L707.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G36890.1; AT4G36890.1; AT4G36890.
GeneID	829842.
GenomeReviews	Gene locus AT4G36890 in contig CT486007_GR.
KEGG	ath:AT4G36890.
NMPDR	fig|3702.1.peg.21793.
Organism-specific databases
TAIR	At4g36890.
Phylogenomic databases
GeneTree	EPGT00070000029587.
InParanoid	Q8L707.
OMA	QGPACNS.
PhylomeDB	Q8L707.
ProtClustDB	CLSN2716060.
Gene expression databases
Genevestigator	Q8L707.
Family and domain databases
InterPro	IPR005027. Glyco_trans_43. [Graphical view]
PANTHER	PTHR10896. Glyco_trans_43. 1 hit.
Pfam	PF03360. Glyco_transf_43. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IRX14_ARATH

Accession

Primary (citable) accession number: Q8L707
Secondary accession number(s): O23194

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program