Reviewed, UniProtKB/Swiss-Prot Q8L6Y1 (UBP14_ARATH)

Names and origin

Protein names

Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 14
    EC=3.1.2.15
Alternative name(s):
    Ubiquitin thioesterase 14
    Ubiquitin-specific-processing protease 14
    Deubiquitinating enzyme 14
      Short name=AtUBP14
    TITAN-6 protein

Gene names

Name:	UBP14
Synonyms:	TTN6
Ordered Locus Names:	At3g20630, At3g20625
ORF Names:	F3H11_1, K10D20.17, K10D20.26

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	797 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. Involved in seed and embryo development. Ref.5 Ref.6
Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Tissue specificity	Constitutively and ubiquitously expressed (at protein level). Ref.5
Sequence similarities	Belongs to the peptidase C19 family. Contains 2 UBA domains. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in seed dormancy Ref.5 Ref.6 Inferred from mutant phenotype. Source: TAIR protein deubiquitination Ref.5 Inferred from direct assay. Source: TAIR ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC ubiquitin-specific protease activity Ref.5 Inferred from direct assay. Source: TAIR zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 797

797

Ubiquitin carboxyl-terminal hydrolase 14

PRO_0000080697

Regions

Domain

613 – 654

42

UBA 1

Domain

670 – 710

41

UBA 2

Zinc finger

178 – 252

75

UBP-type

Sites

Active site

317

1

By similarity

Active site

749

1

By similarity

Active site

758

1

By similarity

Experimental info

Sequence conflict

601

1

G → A in AAG42755. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

797

Helix Strand Turn

Details...

Beta strand

603 – 605

3

Helix

616 – 625

10

Helix

629 – 638

10

Turn

639 – 641

3

Helix

644 – 654

11

Turn

659 – 661

3

Helix

673 – 682

10

Helix

686 – 695

10

Helix

700 – 709

10

Sequences

	Sequence		Length	Mass (Da)
	Q8L6Y1-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: B827C513A6D5C4E2	FASTA	797	88,374
	10 20 30 40 50 60 MELLRSNLSR VQIPEPTHRI YKHECCISFD TPRSEGGLFV DMNSFLAFGK DYVSWNYEKT 70 80 90 100 110 120 GNPVYLHIKQ TRKSIPEDRP LKKPTLLAIG VDGGFDNNEP EYEESYSIVI LPDFVSLPFP 130 140 150 160 170 180 SVELPEKVRI AVDTVVNAVG AERKEQVAAW TAEKKLISEH ALTLQQIKSG IVIPPSGWKC 190 200 210 220 230 240 SKCDKTENLW LNLTDGMILC GRKNWDGTGG NNHAVEHYKE TAYPLAVKLG TITADLEAAD 250 260 270 280 290 300 VYSYPEDDSV LDPLLAEHLA HFGIDFSSMQ KTEMTTAERE LDQNTNFDWN RIQESGKELV 310 320 330 340 350 360 PVFGPGYTGL VNLGNSCYLA ATMQIVFSTH SFISRYFSHQ SLKMAFEMAP ADPTLDLNMQ 370 380 390 400 410 420 LTKLGHGLLS GKYSMPATQK DATTGDPRQE GIPPRMFKNV IAASHAEFSS MRQQDALDFF 430 440 450 460 470 480 LHLVGKVERA SNTTPDLDPS RSFKFGIEEK ILCPSGKVGY NKREDCILSL NIPLHEATNK 490 500 510 520 530 540 DELEAFHKQK AGKGLEENDM RSSDEIVRPR VPLEACLANF ASSEPIEDYY SSALKGMTTA 550 560 570 580 590 600 IKTTGLTSFP DYLVLHMRKF VMEEGWVPKK LDVYIDVPDV IDISHMRSKG LQPGEELLPD 610 620 630 640 650 660 GVPEEVMESA QPVANEEIVA QLVSMGFSQL HCQKAAINTS NAGVEEAMNW LLSHMDDPDI 670 680 690 700 710 720 DAPISHQTSD IDQSSVDTLL SFGFAEDVAR KALKASGGDI EKATDWVFNN PNASVSDMDV 730 740 750 760 770 780 SSSNSAQTPA QSGLPDGGGK YKLFGIVSHM GTSVHCGHYV AHILKEGRWV IFNDDKVGIS 790 TDPPKDMGYV YFFQRLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine." Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D. Plant Physiol. 124:1828-1843(2000) [PubMed: 11115897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The ubiquitin-specific protease UBP14 is essential for early embryo development in Arabidopsis thaliana." Doelling J.H., Yan N., Kurepa J., Walker J., Vierstra R.D. Plant J. 27:393-405(2001) [PubMed: 11576424] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]	"Diversity of TITAN functions in Arabidopsis seed development." Tzafrir I., McElver J.A., Liu C.-M., Yang L.J., Wu J.Q., Martinez A., Patton D.A., Meinke D.W. Plant Physiol. 128:38-51(2002) [PubMed: 11788751] [Abstract] Cited for: FUNCTION.
[7]	"Solution structure of RSGI RUH-011, a UBA domain from Arabidopsis cDNA." RIKEN structural genomics initiative (RSGI) Submitted (SEP-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 594-665.
[8]	"Solution structure of RSGI RUH-023, a UBA domain from Arabidopsis cDNA." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 651-710.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF302664 mRNA. Translation: AAG42755.1.
AP000410, AP002034 Genomic DNA. Translation: BAB01171.1.
AY140096 mRNA. Translation: AAM98237.1.
AK226894 mRNA. Translation: BAE98971.1.

IPI

IPI00525815.

RefSeq

NP_566666.2.

UniGene

At.16942

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VEK	NMR	-	A	594-664	[»]
1WIV	NMR	-	A	651-710	[»]

SMR

Q8L6Y1. Positions 157-272, 298-796.

ModBase

Search...

Protein-protein interaction databases

STRING

Q8L6Y1.

Protein family/group databases

MEROPS

C19.084.

Proteomic databases

PRIDE

Q8L6Y1.

Genome annotation databases

GeneID

821610.

GenomeReviews

Gene locus AT3G20630 in contig BA000014_GR.

KEGG

ath:AT3G20630.

NMPDR

fig|3702.1.peg.14295.

Organism-specific databases

TAIR

At3g20630.

Phylogenomic databases

eggNOG

KOG0944.

HOGENOM

HBG746888.

InParanoid

Q8L6Y1.

OMA

WSTALQA.

PhylomeDB

Q8L6Y1.

Enzyme and pathway databases

BRENDA

3.1.2.15. 302.

Gene expression databases

Genevestigator

Q8L6Y1.

Family and domain databases

InterPro

IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19.
IPR009060. UBA-like.
IPR000449. UBA/transl_elong_EF1B_N.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR016652. Ubiquitinyl_hydrolase.
IPR001607. Znf_UBP.
[Graphical view]

Pfam

PF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]

PIRSF

PIRSF016308. UBP. 1 hit.

SMART

SM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]

PROSITE

PS50030. UBA. 2 hits.
PS00972. UCH_2_1. 1 hit.
PS00973. UCH_2_2. 1 hit.
PS50235. UCH_2_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

UBP14_ARATH

Accession

Primary (citable) accession number: Q8L6Y1
Secondary accession number(s): Q0WV77, Q9FPT0, Q9LJT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)