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Protein

Ubiquitin carboxyl-terminal hydrolase 14

Gene

UBP14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Involved in seed and embryo development.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei317NucleophilePROSITE-ProRule annotation1
Active sitei758Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri178 – 252UBP-typePROSITE-ProRule annotationAdd BLAST75

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • protein deubiquitination Source: TAIR
  • root hair elongation Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes

Protein family/group databases

MEROPSiC19.084

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 14
Short name:
AtUBP14
TITAN-6 protein
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14
Gene namesi
Name:UBP14
Synonyms:TTN6
Ordered Locus Names:At3g20630
ORF Names:F3H11_1, K10D20.17, K10D20.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G20630
TAIRilocus:2083440 AT3G20630

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806971 – 797Ubiquitin carboxyl-terminal hydrolase 14Add BLAST797

Proteomic databases

PaxDbiQ8L6Y1
PRIDEiQ8L6Y1

PTM databases

iPTMnetiQ8L6Y1

Expressioni

Tissue specificityi

Constitutively and ubiquitously expressed (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ8L6Y1 baseline and differential
GenevisibleiQ8L6Y1 AT

Interactioni

Protein-protein interaction databases

BioGridi6942, 1 interactor
STRINGi3702.AT3G20630.1

Structurei

Secondary structure

1797
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi603 – 605Combined sources3
Helixi616 – 625Combined sources10
Helixi629 – 638Combined sources10
Turni639 – 641Combined sources3
Helixi644 – 654Combined sources11
Turni659 – 661Combined sources3
Helixi673 – 682Combined sources10
Helixi686 – 695Combined sources10
Helixi700 – 709Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VEKNMR-A594-664[»]
1WIVNMR-A651-710[»]
ProteinModelPortaliQ8L6Y1
SMRiQ8L6Y1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8L6Y1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini308 – 796USPAdd BLAST489
Domaini613 – 654UBA 1PROSITE-ProRule annotationAdd BLAST42
Domaini670 – 710UBA 2PROSITE-ProRule annotationAdd BLAST41

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri178 – 252UBP-typePROSITE-ProRule annotationAdd BLAST75

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0944 Eukaryota
COG5207 LUCA
HOGENOMiHOG000162311
InParanoidiQ8L6Y1
KOiK11836
OMAiMKEEHKP
OrthoDBiEOG093603YS
PhylomeDBiQ8L6Y1

Family and domain databases

Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR001394 Peptidase_C19_UCH
IPR015940 UBA
IPR009060 UBA-like_sf
IPR016652 Ubiquitinyl_hydrolase
IPR018200 USP_CS
IPR028889 USP_dom
IPR013083 Znf_RING/FYVE/PHD
IPR001607 Znf_UBP
PfamiView protein in Pfam
PF00627 UBA, 2 hits
PF00443 UCH, 1 hit
PF02148 zf-UBP, 1 hit
PIRSFiPIRSF016308 UBP, 1 hit
SMARTiView protein in SMART
SM00165 UBA, 2 hits
SM00290 ZnF_UBP, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 2 hits
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit
PS50271 ZF_UBP, 1 hit

Sequencei

Sequence statusi: Complete.

Q8L6Y1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLRSNLSR VQIPEPTHRI YKHECCISFD TPRSEGGLFV DMNSFLAFGK
60 70 80 90 100
DYVSWNYEKT GNPVYLHIKQ TRKSIPEDRP LKKPTLLAIG VDGGFDNNEP
110 120 130 140 150
EYEESYSIVI LPDFVSLPFP SVELPEKVRI AVDTVVNAVG AERKEQVAAW
160 170 180 190 200
TAEKKLISEH ALTLQQIKSG IVIPPSGWKC SKCDKTENLW LNLTDGMILC
210 220 230 240 250
GRKNWDGTGG NNHAVEHYKE TAYPLAVKLG TITADLEAAD VYSYPEDDSV
260 270 280 290 300
LDPLLAEHLA HFGIDFSSMQ KTEMTTAERE LDQNTNFDWN RIQESGKELV
310 320 330 340 350
PVFGPGYTGL VNLGNSCYLA ATMQIVFSTH SFISRYFSHQ SLKMAFEMAP
360 370 380 390 400
ADPTLDLNMQ LTKLGHGLLS GKYSMPATQK DATTGDPRQE GIPPRMFKNV
410 420 430 440 450
IAASHAEFSS MRQQDALDFF LHLVGKVERA SNTTPDLDPS RSFKFGIEEK
460 470 480 490 500
ILCPSGKVGY NKREDCILSL NIPLHEATNK DELEAFHKQK AGKGLEENDM
510 520 530 540 550
RSSDEIVRPR VPLEACLANF ASSEPIEDYY SSALKGMTTA IKTTGLTSFP
560 570 580 590 600
DYLVLHMRKF VMEEGWVPKK LDVYIDVPDV IDISHMRSKG LQPGEELLPD
610 620 630 640 650
GVPEEVMESA QPVANEEIVA QLVSMGFSQL HCQKAAINTS NAGVEEAMNW
660 670 680 690 700
LLSHMDDPDI DAPISHQTSD IDQSSVDTLL SFGFAEDVAR KALKASGGDI
710 720 730 740 750
EKATDWVFNN PNASVSDMDV SSSNSAQTPA QSGLPDGGGK YKLFGIVSHM
760 770 780 790
GTSVHCGHYV AHILKEGRWV IFNDDKVGIS TDPPKDMGYV YFFQRLD
Length:797
Mass (Da):88,374
Last modified:October 1, 2002 - v1
Checksum:iB827C513A6D5C4E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti601G → A in AAG42755 (PubMed:11115897).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302664 mRNA Translation: AAG42755.1
AP000410, AP002034 Genomic DNA Translation: BAB01171.1
CP002686 Genomic DNA Translation: AEE76405.1
AY140096 mRNA Translation: AAM98237.1
AK226894 mRNA Translation: BAE98971.1
RefSeqiNP_566666.2, NM_112954.4
UniGeneiAt.16942

Genome annotation databases

EnsemblPlantsiAT3G20630.1; AT3G20630.1; AT3G20630
GeneIDi821610
GrameneiAT3G20630.1; AT3G20630.1; AT3G20630
KEGGiath:AT3G20630

Similar proteinsi

Entry informationi

Entry nameiUBP14_ARATH
AccessioniPrimary (citable) accession number: Q8L6Y1
Secondary accession number(s): Q0WV77, Q9FPT0, Q9LJT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2002
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health