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Q8L6Y1 (UBP14_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 14

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 14
Short name=AtUBP14
TITAN-6 protein
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14
Gene names
Name:UBP14
Synonyms:TTN6
Ordered Locus Names:At3g20630, At3g20625
ORF Names:F3H11_1, K10D20.17, K10D20.26
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length797 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Involved in seed and embryo development. Ref.6 Ref.7

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Tissue specificity

Constitutively and ubiquitously expressed (at protein level). Ref.6

Sequence similarities

Belongs to the peptidase C19 family.

Contains 2 UBA domains.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 797797Ubiquitin carboxyl-terminal hydrolase 14
PRO_0000080697

Regions

Domain308 – 796489USP
Domain613 – 65442UBA 1
Domain670 – 71041UBA 2
Zinc finger178 – 25275UBP-type

Sites

Active site3171Nucleophile By similarity
Active site7581Proton acceptor By similarity

Experimental info

Sequence conflict6011G → A in AAG42755. Ref.1

Secondary structure

.................. 797
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8L6Y1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: B827C513A6D5C4E2

FASTA79788,374
        10         20         30         40         50         60 
MELLRSNLSR VQIPEPTHRI YKHECCISFD TPRSEGGLFV DMNSFLAFGK DYVSWNYEKT 

        70         80         90        100        110        120 
GNPVYLHIKQ TRKSIPEDRP LKKPTLLAIG VDGGFDNNEP EYEESYSIVI LPDFVSLPFP 

       130        140        150        160        170        180 
SVELPEKVRI AVDTVVNAVG AERKEQVAAW TAEKKLISEH ALTLQQIKSG IVIPPSGWKC 

       190        200        210        220        230        240 
SKCDKTENLW LNLTDGMILC GRKNWDGTGG NNHAVEHYKE TAYPLAVKLG TITADLEAAD 

       250        260        270        280        290        300 
VYSYPEDDSV LDPLLAEHLA HFGIDFSSMQ KTEMTTAERE LDQNTNFDWN RIQESGKELV 

       310        320        330        340        350        360 
PVFGPGYTGL VNLGNSCYLA ATMQIVFSTH SFISRYFSHQ SLKMAFEMAP ADPTLDLNMQ 

       370        380        390        400        410        420 
LTKLGHGLLS GKYSMPATQK DATTGDPRQE GIPPRMFKNV IAASHAEFSS MRQQDALDFF 

       430        440        450        460        470        480 
LHLVGKVERA SNTTPDLDPS RSFKFGIEEK ILCPSGKVGY NKREDCILSL NIPLHEATNK 

       490        500        510        520        530        540 
DELEAFHKQK AGKGLEENDM RSSDEIVRPR VPLEACLANF ASSEPIEDYY SSALKGMTTA 

       550        560        570        580        590        600 
IKTTGLTSFP DYLVLHMRKF VMEEGWVPKK LDVYIDVPDV IDISHMRSKG LQPGEELLPD 

       610        620        630        640        650        660 
GVPEEVMESA QPVANEEIVA QLVSMGFSQL HCQKAAINTS NAGVEEAMNW LLSHMDDPDI 

       670        680        690        700        710        720 
DAPISHQTSD IDQSSVDTLL SFGFAEDVAR KALKASGGDI EKATDWVFNN PNASVSDMDV 

       730        740        750        760        770        780 
SSSNSAQTPA QSGLPDGGGK YKLFGIVSHM GTSVHCGHYV AHILKEGRWV IFNDDKVGIS 

       790 
TDPPKDMGYV YFFQRLD 

« Hide

References

« Hide 'large scale' references
[1]"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"The ubiquitin-specific protease UBP14 is essential for early embryo development in Arabidopsis thaliana."
Doelling J.H., Yan N., Kurepa J., Walker J., Vierstra R.D.
Plant J. 27:393-405(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]"Diversity of TITAN functions in Arabidopsis seed development."
Tzafrir I., McElver J.A., Liu C.-M., Yang L.J., Wu J.Q., Martinez A., Patton D.A., Meinke D.W.
Plant Physiol. 128:38-51(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Solution structure of RSGI RUH-011, a UBA domain from Arabidopsis cDNA."
RIKEN structural genomics initiative (RSGI)
Submitted (SEP-2004) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 594-665.
[9]"Solution structure of RSGI RUH-023, a UBA domain from Arabidopsis cDNA."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2004) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 651-710.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF302664 mRNA. Translation: AAG42755.1.
AP000410, AP002034 Genomic DNA. Translation: BAB01171.1.
CP002686 Genomic DNA. Translation: AEE76405.1.
AY140096 mRNA. Translation: AAM98237.1.
AK226894 mRNA. Translation: BAE98971.1.
RefSeqNP_566666.2. NM_112954.3.
UniGeneAt.16942.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VEKNMR-A594-664[»]
1WIVNMR-A651-710[»]
ProteinModelPortalQ8L6Y1.
SMRQ8L6Y1. Positions 2-796.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid6942. 1 interaction.

Protein family/group databases

MEROPSC19.084.

Proteomic databases

PaxDbQ8L6Y1.
PRIDEQ8L6Y1.

Protocols and materials databases

DNASU821610.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G20630.1; AT3G20630.1; AT3G20630.
GeneID821610.
KEGGath:AT3G20630.

Organism-specific databases

TAIRAT3G20630.

Phylogenomic databases

eggNOGCOG5207.
HOGENOMHOG000162311.
InParanoidQ8L6Y1.
KOK11836.
OMALRWEFSM.
PhylomeDBQ8L6Y1.
ProtClustDBCLSN2690557.

Enzyme and pathway databases

BioCycARA:AT3G20630-MONOMER.

Gene expression databases

GenevestigatorQ8L6Y1.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFPIRSF016308. UBP. 1 hit.
SMARTSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMSSF46934. SSF46934. 1 hit.
PROSITEPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8L6Y1.
PROQ8L6Y1.

Entry information

Entry nameUBP14_ARATH
AccessionPrimary (citable) accession number: Q8L6Y1
Secondary accession number(s): Q0WV77, Q9FPT0, Q9LJT6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names