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Q8L6Y1

- UBP14_ARATH

UniProt

Q8L6Y1 - UBP14_ARATH

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Protein

Ubiquitin carboxyl-terminal hydrolase 14

Gene

UBP14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Involved in seed and embryo development.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei317 – 3171NucleophilePROSITE-ProRule annotation
Active sitei758 – 7581Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri178 – 25275UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. omega peptidase activity Source: InterPro
  2. ubiquitin-specific protease activity Source: TAIR
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. embryo development ending in seed dormancy Source: TAIR
  2. protein deubiquitination Source: TAIR
  3. root hair elongation Source: TAIR
  4. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G20630-MONOMER.

Protein family/group databases

MEROPSiC19.084.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 14
Short name:
AtUBP14
TITAN-6 protein
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14
Gene namesi
Name:UBP14
Synonyms:TTN6
Ordered Locus Names:At3g20630, At3g20625
ORF Names:F3H11_1, K10D20.17, K10D20.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G20630.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 797797Ubiquitin carboxyl-terminal hydrolase 14PRO_0000080697Add
BLAST

Proteomic databases

PaxDbiQ8L6Y1.
PRIDEiQ8L6Y1.

Expressioni

Tissue specificityi

Constitutively and ubiquitously expressed (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ8L6Y1.

Interactioni

Protein-protein interaction databases

BioGridi6942. 1 interaction.

Structurei

Secondary structure

1
797
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi603 – 6053
Helixi616 – 62510
Helixi629 – 63810
Turni639 – 6413
Helixi644 – 65411
Turni659 – 6613
Helixi673 – 68210
Helixi686 – 69510
Helixi700 – 70910

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VEKNMR-A594-664[»]
1WIVNMR-A651-710[»]
ProteinModelPortaliQ8L6Y1.
SMRiQ8L6Y1. Positions 2-796.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8L6Y1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini308 – 796489USPAdd
BLAST
Domaini613 – 65442UBA 1PROSITE-ProRule annotationAdd
BLAST
Domaini670 – 71041UBA 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 2 UBA domains.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri178 – 25275UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5207.
HOGENOMiHOG000162311.
InParanoidiQ8L6Y1.
KOiK11836.
OMAiDDMVEDP.
PhylomeDBiQ8L6Y1.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8L6Y1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELLRSNLSR VQIPEPTHRI YKHECCISFD TPRSEGGLFV DMNSFLAFGK
60 70 80 90 100
DYVSWNYEKT GNPVYLHIKQ TRKSIPEDRP LKKPTLLAIG VDGGFDNNEP
110 120 130 140 150
EYEESYSIVI LPDFVSLPFP SVELPEKVRI AVDTVVNAVG AERKEQVAAW
160 170 180 190 200
TAEKKLISEH ALTLQQIKSG IVIPPSGWKC SKCDKTENLW LNLTDGMILC
210 220 230 240 250
GRKNWDGTGG NNHAVEHYKE TAYPLAVKLG TITADLEAAD VYSYPEDDSV
260 270 280 290 300
LDPLLAEHLA HFGIDFSSMQ KTEMTTAERE LDQNTNFDWN RIQESGKELV
310 320 330 340 350
PVFGPGYTGL VNLGNSCYLA ATMQIVFSTH SFISRYFSHQ SLKMAFEMAP
360 370 380 390 400
ADPTLDLNMQ LTKLGHGLLS GKYSMPATQK DATTGDPRQE GIPPRMFKNV
410 420 430 440 450
IAASHAEFSS MRQQDALDFF LHLVGKVERA SNTTPDLDPS RSFKFGIEEK
460 470 480 490 500
ILCPSGKVGY NKREDCILSL NIPLHEATNK DELEAFHKQK AGKGLEENDM
510 520 530 540 550
RSSDEIVRPR VPLEACLANF ASSEPIEDYY SSALKGMTTA IKTTGLTSFP
560 570 580 590 600
DYLVLHMRKF VMEEGWVPKK LDVYIDVPDV IDISHMRSKG LQPGEELLPD
610 620 630 640 650
GVPEEVMESA QPVANEEIVA QLVSMGFSQL HCQKAAINTS NAGVEEAMNW
660 670 680 690 700
LLSHMDDPDI DAPISHQTSD IDQSSVDTLL SFGFAEDVAR KALKASGGDI
710 720 730 740 750
EKATDWVFNN PNASVSDMDV SSSNSAQTPA QSGLPDGGGK YKLFGIVSHM
760 770 780 790
GTSVHCGHYV AHILKEGRWV IFNDDKVGIS TDPPKDMGYV YFFQRLD
Length:797
Mass (Da):88,374
Last modified:October 1, 2002 - v1
Checksum:iB827C513A6D5C4E2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti601 – 6011G → A in AAG42755. (PubMed:11115897)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302664 mRNA. Translation: AAG42755.1.
AP000410, AP002034 Genomic DNA. Translation: BAB01171.1.
CP002686 Genomic DNA. Translation: AEE76405.1.
AY140096 mRNA. Translation: AAM98237.1.
AK226894 mRNA. Translation: BAE98971.1.
RefSeqiNP_566666.2. NM_112954.3.
UniGeneiAt.16942.

Genome annotation databases

EnsemblPlantsiAT3G20630.1; AT3G20630.1; AT3G20630.
GeneIDi821610.
KEGGiath:AT3G20630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302664 mRNA. Translation: AAG42755.1 .
AP000410 , AP002034 Genomic DNA. Translation: BAB01171.1 .
CP002686 Genomic DNA. Translation: AEE76405.1 .
AY140096 mRNA. Translation: AAM98237.1 .
AK226894 mRNA. Translation: BAE98971.1 .
RefSeqi NP_566666.2. NM_112954.3.
UniGenei At.16942.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VEK NMR - A 594-664 [» ]
1WIV NMR - A 651-710 [» ]
ProteinModelPortali Q8L6Y1.
SMRi Q8L6Y1. Positions 2-796.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 6942. 1 interaction.

Protein family/group databases

MEROPSi C19.084.

Proteomic databases

PaxDbi Q8L6Y1.
PRIDEi Q8L6Y1.

Protocols and materials databases

DNASUi 821610.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G20630.1 ; AT3G20630.1 ; AT3G20630 .
GeneIDi 821610.
KEGGi ath:AT3G20630.

Organism-specific databases

TAIRi AT3G20630.

Phylogenomic databases

eggNOGi COG5207.
HOGENOMi HOG000162311.
InParanoidi Q8L6Y1.
KOi K11836.
OMAi DDMVEDP.
PhylomeDBi Q8L6Y1.

Enzyme and pathway databases

BioCyci ARA:AT3G20630-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q8L6Y1.
PROi Q8L6Y1.

Gene expression databases

Genevestigatori Q8L6Y1.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PIRSFi PIRSF016308. UBP. 1 hit.
SMARTi SM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view ]
SUPFAMi SSF46934. SSF46934. 1 hit.
PROSITEi PS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "The ubiquitin-specific protease UBP14 is essential for early embryo development in Arabidopsis thaliana."
    Doelling J.H., Yan N., Kurepa J., Walker J., Vierstra R.D.
    Plant J. 27:393-405(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  7. Cited for: FUNCTION.
  8. "Solution structure of RSGI RUH-011, a UBA domain from Arabidopsis cDNA."
    RIKEN structural genomics initiative (RSGI)
    Submitted (SEP-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 594-665.
  9. "Solution structure of RSGI RUH-023, a UBA domain from Arabidopsis cDNA."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 651-710.

Entry informationi

Entry nameiUBP14_ARATH
AccessioniPrimary (citable) accession number: Q8L6Y1
Secondary accession number(s): Q0WV77, Q9FPT0, Q9LJT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2002
Last modified: October 1, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3