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Q8L6Y1

- UBP14_ARATH

UniProt

Q8L6Y1 - UBP14_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 14

Gene

UBP14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Involved in seed and embryo development.2 Publications

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei317 – 3171NucleophilePROSITE-ProRule annotation
    Active sitei758 – 7581Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri178 – 25275UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. omega peptidase activity Source: InterPro
    2. ubiquitin-specific protease activity Source: TAIR
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. embryo development ending in seed dormancy Source: TAIR
    2. protein deubiquitination Source: TAIR
    3. root hair elongation Source: TAIR
    4. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT3G20630-MONOMER.

    Protein family/group databases

    MEROPSiC19.084.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 14
    Short name:
    AtUBP14
    TITAN-6 protein
    Ubiquitin thioesterase 14
    Ubiquitin-specific-processing protease 14
    Gene namesi
    Name:UBP14
    Synonyms:TTN6
    Ordered Locus Names:At3g20630, At3g20625
    ORF Names:F3H11_1, K10D20.17, K10D20.26
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G20630.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 797797Ubiquitin carboxyl-terminal hydrolase 14PRO_0000080697Add
    BLAST

    Proteomic databases

    PaxDbiQ8L6Y1.
    PRIDEiQ8L6Y1.

    Expressioni

    Tissue specificityi

    Constitutively and ubiquitously expressed (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriQ8L6Y1.

    Interactioni

    Protein-protein interaction databases

    BioGridi6942. 1 interaction.

    Structurei

    Secondary structure

    1
    797
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi603 – 6053
    Helixi616 – 62510
    Helixi629 – 63810
    Turni639 – 6413
    Helixi644 – 65411
    Turni659 – 6613
    Helixi673 – 68210
    Helixi686 – 69510
    Helixi700 – 70910

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VEKNMR-A594-664[»]
    1WIVNMR-A651-710[»]
    ProteinModelPortaliQ8L6Y1.
    SMRiQ8L6Y1. Positions 2-796.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8L6Y1.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini308 – 796489USPAdd
    BLAST
    Domaini613 – 65442UBA 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini670 – 71041UBA 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 2 UBA domains.PROSITE-ProRule annotation
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri178 – 25275UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5207.
    HOGENOMiHOG000162311.
    InParanoidiQ8L6Y1.
    KOiK11836.
    OMAiDDMVEDP.
    PhylomeDBiQ8L6Y1.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR016652. Ubiquitinyl_hydrolase.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00627. UBA. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016308. UBP. 1 hit.
    SMARTiSM00165. UBA. 2 hits.
    SM00290. ZnF_UBP. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    PROSITEiPS50030. UBA. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8L6Y1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELLRSNLSR VQIPEPTHRI YKHECCISFD TPRSEGGLFV DMNSFLAFGK    50
    DYVSWNYEKT GNPVYLHIKQ TRKSIPEDRP LKKPTLLAIG VDGGFDNNEP 100
    EYEESYSIVI LPDFVSLPFP SVELPEKVRI AVDTVVNAVG AERKEQVAAW 150
    TAEKKLISEH ALTLQQIKSG IVIPPSGWKC SKCDKTENLW LNLTDGMILC 200
    GRKNWDGTGG NNHAVEHYKE TAYPLAVKLG TITADLEAAD VYSYPEDDSV 250
    LDPLLAEHLA HFGIDFSSMQ KTEMTTAERE LDQNTNFDWN RIQESGKELV 300
    PVFGPGYTGL VNLGNSCYLA ATMQIVFSTH SFISRYFSHQ SLKMAFEMAP 350
    ADPTLDLNMQ LTKLGHGLLS GKYSMPATQK DATTGDPRQE GIPPRMFKNV 400
    IAASHAEFSS MRQQDALDFF LHLVGKVERA SNTTPDLDPS RSFKFGIEEK 450
    ILCPSGKVGY NKREDCILSL NIPLHEATNK DELEAFHKQK AGKGLEENDM 500
    RSSDEIVRPR VPLEACLANF ASSEPIEDYY SSALKGMTTA IKTTGLTSFP 550
    DYLVLHMRKF VMEEGWVPKK LDVYIDVPDV IDISHMRSKG LQPGEELLPD 600
    GVPEEVMESA QPVANEEIVA QLVSMGFSQL HCQKAAINTS NAGVEEAMNW 650
    LLSHMDDPDI DAPISHQTSD IDQSSVDTLL SFGFAEDVAR KALKASGGDI 700
    EKATDWVFNN PNASVSDMDV SSSNSAQTPA QSGLPDGGGK YKLFGIVSHM 750
    GTSVHCGHYV AHILKEGRWV IFNDDKVGIS TDPPKDMGYV YFFQRLD 797
    Length:797
    Mass (Da):88,374
    Last modified:October 1, 2002 - v1
    Checksum:iB827C513A6D5C4E2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti601 – 6011G → A in AAG42755. (PubMed:11115897)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302664 mRNA. Translation: AAG42755.1.
    AP000410, AP002034 Genomic DNA. Translation: BAB01171.1.
    CP002686 Genomic DNA. Translation: AEE76405.1.
    AY140096 mRNA. Translation: AAM98237.1.
    AK226894 mRNA. Translation: BAE98971.1.
    RefSeqiNP_566666.2. NM_112954.3.
    UniGeneiAt.16942.

    Genome annotation databases

    EnsemblPlantsiAT3G20630.1; AT3G20630.1; AT3G20630.
    GeneIDi821610.
    KEGGiath:AT3G20630.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302664 mRNA. Translation: AAG42755.1 .
    AP000410 , AP002034 Genomic DNA. Translation: BAB01171.1 .
    CP002686 Genomic DNA. Translation: AEE76405.1 .
    AY140096 mRNA. Translation: AAM98237.1 .
    AK226894 mRNA. Translation: BAE98971.1 .
    RefSeqi NP_566666.2. NM_112954.3.
    UniGenei At.16942.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VEK NMR - A 594-664 [» ]
    1WIV NMR - A 651-710 [» ]
    ProteinModelPortali Q8L6Y1.
    SMRi Q8L6Y1. Positions 2-796.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 6942. 1 interaction.

    Protein family/group databases

    MEROPSi C19.084.

    Proteomic databases

    PaxDbi Q8L6Y1.
    PRIDEi Q8L6Y1.

    Protocols and materials databases

    DNASUi 821610.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G20630.1 ; AT3G20630.1 ; AT3G20630 .
    GeneIDi 821610.
    KEGGi ath:AT3G20630.

    Organism-specific databases

    TAIRi AT3G20630.

    Phylogenomic databases

    eggNOGi COG5207.
    HOGENOMi HOG000162311.
    InParanoidi Q8L6Y1.
    KOi K11836.
    OMAi DDMVEDP.
    PhylomeDBi Q8L6Y1.

    Enzyme and pathway databases

    BioCyci ARA:AT3G20630-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q8L6Y1.
    PROi Q8L6Y1.

    Gene expression databases

    Genevestigatori Q8L6Y1.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR016652. Ubiquitinyl_hydrolase.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00627. UBA. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016308. UBP. 1 hit.
    SMARTi SM00165. UBA. 2 hits.
    SM00290. ZnF_UBP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    PROSITEi PS50030. UBA. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "The ubiquitin-specific protease UBP14 is essential for early embryo development in Arabidopsis thaliana."
      Doelling J.H., Yan N., Kurepa J., Walker J., Vierstra R.D.
      Plant J. 27:393-405(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    7. Cited for: FUNCTION.
    8. "Solution structure of RSGI RUH-011, a UBA domain from Arabidopsis cDNA."
      RIKEN structural genomics initiative (RSGI)
      Submitted (SEP-2004) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 594-665.
    9. "Solution structure of RSGI RUH-023, a UBA domain from Arabidopsis cDNA."
      RIKEN structural genomics initiative (RSGI)
      Submitted (NOV-2004) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 651-710.

    Entry informationi

    Entry nameiUBP14_ARATH
    AccessioniPrimary (citable) accession number: Q8L6Y1
    Secondary accession number(s): Q0WV77, Q9FPT0, Q9LJT6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3