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Protein

Ubiquitin carboxyl-terminal hydrolase 14

Gene

UBP14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Involved in seed and embryo development.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei317NucleophilePROSITE-ProRule annotation1
Active sitei758Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri178 – 252UBP-typePROSITE-ProRule annotationAdd BLAST75

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • protein deubiquitination Source: TAIR
  • root hair elongation Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G20630-MONOMER.
ReactomeiR-ATH-5689880. Ub-specific processing proteases.
R-ATH-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Protein family/group databases

MEROPSiC19.084.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 14
Short name:
AtUBP14
TITAN-6 protein
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14
Gene namesi
Name:UBP14
Synonyms:TTN6
Ordered Locus Names:At3g20630, At3g20625
ORF Names:F3H11_1, K10D20.17, K10D20.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G20630.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806971 – 797Ubiquitin carboxyl-terminal hydrolase 14Add BLAST797

Proteomic databases

PaxDbiQ8L6Y1.
PRIDEiQ8L6Y1.

PTM databases

iPTMnetiQ8L6Y1.

Expressioni

Tissue specificityi

Constitutively and ubiquitously expressed (at protein level).1 Publication

Gene expression databases

GenevisibleiQ8L6Y1. AT.

Interactioni

Protein-protein interaction databases

BioGridi6942. 1 interactor.
STRINGi3702.AT3G20630.1.

Structurei

Secondary structure

1797
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi603 – 605Combined sources3
Helixi616 – 625Combined sources10
Helixi629 – 638Combined sources10
Turni639 – 641Combined sources3
Helixi644 – 654Combined sources11
Turni659 – 661Combined sources3
Helixi673 – 682Combined sources10
Helixi686 – 695Combined sources10
Helixi700 – 709Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VEKNMR-A594-664[»]
1WIVNMR-A651-710[»]
ProteinModelPortaliQ8L6Y1.
SMRiQ8L6Y1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8L6Y1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini308 – 796USPAdd BLAST489
Domaini613 – 654UBA 1PROSITE-ProRule annotationAdd BLAST42
Domaini670 – 710UBA 2PROSITE-ProRule annotationAdd BLAST41

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 2 UBA domains.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri178 – 252UBP-typePROSITE-ProRule annotationAdd BLAST75

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0944. Eukaryota.
COG5207. LUCA.
HOGENOMiHOG000162311.
InParanoidiQ8L6Y1.
KOiK11836.
OMAiGQRAYLH.
OrthoDBiEOG093603YS.
PhylomeDBiQ8L6Y1.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR015940. UBA.
IPR009060. UBA-like.
IPR016652. Ubiquitinyl_hydrolase.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8L6Y1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLRSNLSR VQIPEPTHRI YKHECCISFD TPRSEGGLFV DMNSFLAFGK
60 70 80 90 100
DYVSWNYEKT GNPVYLHIKQ TRKSIPEDRP LKKPTLLAIG VDGGFDNNEP
110 120 130 140 150
EYEESYSIVI LPDFVSLPFP SVELPEKVRI AVDTVVNAVG AERKEQVAAW
160 170 180 190 200
TAEKKLISEH ALTLQQIKSG IVIPPSGWKC SKCDKTENLW LNLTDGMILC
210 220 230 240 250
GRKNWDGTGG NNHAVEHYKE TAYPLAVKLG TITADLEAAD VYSYPEDDSV
260 270 280 290 300
LDPLLAEHLA HFGIDFSSMQ KTEMTTAERE LDQNTNFDWN RIQESGKELV
310 320 330 340 350
PVFGPGYTGL VNLGNSCYLA ATMQIVFSTH SFISRYFSHQ SLKMAFEMAP
360 370 380 390 400
ADPTLDLNMQ LTKLGHGLLS GKYSMPATQK DATTGDPRQE GIPPRMFKNV
410 420 430 440 450
IAASHAEFSS MRQQDALDFF LHLVGKVERA SNTTPDLDPS RSFKFGIEEK
460 470 480 490 500
ILCPSGKVGY NKREDCILSL NIPLHEATNK DELEAFHKQK AGKGLEENDM
510 520 530 540 550
RSSDEIVRPR VPLEACLANF ASSEPIEDYY SSALKGMTTA IKTTGLTSFP
560 570 580 590 600
DYLVLHMRKF VMEEGWVPKK LDVYIDVPDV IDISHMRSKG LQPGEELLPD
610 620 630 640 650
GVPEEVMESA QPVANEEIVA QLVSMGFSQL HCQKAAINTS NAGVEEAMNW
660 670 680 690 700
LLSHMDDPDI DAPISHQTSD IDQSSVDTLL SFGFAEDVAR KALKASGGDI
710 720 730 740 750
EKATDWVFNN PNASVSDMDV SSSNSAQTPA QSGLPDGGGK YKLFGIVSHM
760 770 780 790
GTSVHCGHYV AHILKEGRWV IFNDDKVGIS TDPPKDMGYV YFFQRLD
Length:797
Mass (Da):88,374
Last modified:October 1, 2002 - v1
Checksum:iB827C513A6D5C4E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti601G → A in AAG42755 (PubMed:11115897).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302664 mRNA. Translation: AAG42755.1.
AP000410, AP002034 Genomic DNA. Translation: BAB01171.1.
CP002686 Genomic DNA. Translation: AEE76405.1.
AY140096 mRNA. Translation: AAM98237.1.
AK226894 mRNA. Translation: BAE98971.1.
RefSeqiNP_566666.2. NM_112954.4.
UniGeneiAt.16942.

Genome annotation databases

EnsemblPlantsiAT3G20630.1; AT3G20630.1; AT3G20630.
GeneIDi821610.
GrameneiAT3G20630.1; AT3G20630.1; AT3G20630.
KEGGiath:AT3G20630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302664 mRNA. Translation: AAG42755.1.
AP000410, AP002034 Genomic DNA. Translation: BAB01171.1.
CP002686 Genomic DNA. Translation: AEE76405.1.
AY140096 mRNA. Translation: AAM98237.1.
AK226894 mRNA. Translation: BAE98971.1.
RefSeqiNP_566666.2. NM_112954.4.
UniGeneiAt.16942.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VEKNMR-A594-664[»]
1WIVNMR-A651-710[»]
ProteinModelPortaliQ8L6Y1.
SMRiQ8L6Y1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6942. 1 interactor.
STRINGi3702.AT3G20630.1.

Protein family/group databases

MEROPSiC19.084.

PTM databases

iPTMnetiQ8L6Y1.

Proteomic databases

PaxDbiQ8L6Y1.
PRIDEiQ8L6Y1.

Protocols and materials databases

DNASUi821610.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G20630.1; AT3G20630.1; AT3G20630.
GeneIDi821610.
GrameneiAT3G20630.1; AT3G20630.1; AT3G20630.
KEGGiath:AT3G20630.

Organism-specific databases

TAIRiAT3G20630.

Phylogenomic databases

eggNOGiKOG0944. Eukaryota.
COG5207. LUCA.
HOGENOMiHOG000162311.
InParanoidiQ8L6Y1.
KOiK11836.
OMAiGQRAYLH.
OrthoDBiEOG093603YS.
PhylomeDBiQ8L6Y1.

Enzyme and pathway databases

BioCyciARA:AT3G20630-MONOMER.
ReactomeiR-ATH-5689880. Ub-specific processing proteases.
R-ATH-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Miscellaneous databases

EvolutionaryTraceiQ8L6Y1.
PROiQ8L6Y1.

Gene expression databases

GenevisibleiQ8L6Y1. AT.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR015940. UBA.
IPR009060. UBA-like.
IPR016652. Ubiquitinyl_hydrolase.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP14_ARATH
AccessioniPrimary (citable) accession number: Q8L6Y1
Secondary accession number(s): Q0WV77, Q9FPT0, Q9LJT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.