Reviewed, UniProtKB/Swiss-Prot Q8L6J5 (RPO1B_TOBAC)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase 1B, mitochondrial
    EC=2.7.7.6
Alternative name(s):
    T7 bacteriophage-type single subunit RNA polymerase 1B
    NictaRpoT1-tom

Gene names

Name:

RPOT1-TOM

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	1002 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Mitochondrion. Ref.1
Sequence similarities	Belongs to the phage and mitochondrial RNA polymerase family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Mitochondrion
Domain	Transit peptide
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 21	21	Mitochondrion Potential
	Chain	22 – 1002	981	DNA-directed RNA polymerase 1B, mitochondrial		PRO_0000046033
Sites
	Active site	703	1	By similarity
	Active site	778	1	By similarity
	Active site	935	1	By similarity
Experimental info
	Sequence conflict	502	1	D → N in CAC82574. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8L6J5-1 [UniParc]. Last modified February 7, 2006. Version 2. Checksum: 06451D1652707D72	FASTA	1,002	113,532
	10 20 30 40 50 60 MWRYISKHAY SRKFRNSHDS ALLGFSQYSS SFGKTRPLQC LCEESTTHPN LGLSQNSIFS 70 80 90 100 110 120 RISRKVRHLE GICEESSKNP HLGLSQNSTF SSVKGDFRIC GKRGSGSLGR LRSYGSAAEA 130 140 150 160 170 180 IVSTSEEDID EIQELIEEMD KENEALKANL QPKQPKTIGG MGVGKYNFLR RRQIKVETEA 190 200 210 220 230 240 WEEAAKEYQE LLMDMCEQKL APNLPYMKSL FLGWFEPLRD AIAAEQKLCD EGKNRGAYAP 250 260 270 280 290 300 FFDQLPAEMM AVITMHKLMG LLMTGGGTGS ARVVQAASYI GEAIEHEARI HRFLEKTKKS 310 320 330 340 350 360 NALSGDLEET PGDMMKERER LRKKVKILMK KQKLRQVRKI VKQQDDEKPW GQDNLVKVGC 370 380 390 400 410 420 RLIQILMETA YIQPPNDQLD DGPPDIRPAF VHTLKTVETM KGSRRYGVIQ CDPLVRKGLD 430 440 450 460 470 480 KTARHMVIPY MPMLVPPQSW LGYDKGGYLF LPSYIMRTHG AKQQREAVKR VPKKQLEPVF 490 500 510 520 530 540 QALDTLGNTK WRVNRKVLGI VDRIWASGGR LADLVDREDV PLPEAPDTED EAEIRKWKWK 550 560 570 580 590 600 VKGVKKENCE RHSQRCDIEL KLAVARKMKD EDGFYYPHNL DFRGRAYPMH PYLNHLGSDL 610 620 630 640 650 660 CRGILEFAEG RPLGTSGLRW LKIHLANVYG GGVDKLSYEG RVAFSENHLE DIFDSAERPL 670 680 690 700 710 720 EGKRWWLGAE DPFQCLATCI NIAEALRSPS PETAISYMPI HQDGSCNGLQ HYAALGRDKL 730 740 750 760 770 780 GAAAVNLVAG DKPADVYSGI AARVLDIMKR DAAKDPANDP NVMRARLLIN QVDRKLVKQT 790 800 810 820 830 840 VMTSVYGVTY IGARDQIKKR LKERGVIEDD NELFAAACYA AKTTLTALGE MFEAARSIMS 850 860 870 880 890 900 WLGDCAKIIA MENHPVRWTT PLGLPVVQPY RKLGRHLIKT SLQILTLQRE TDKVMVKRQR 910 920 930 940 950 960 TAFPPNFVHS LDGSHMMMTA IACKESGLSF AGVHDSYWTH ACDVDQMNKI LREKFVELYD 970 980 990 1000 APILENLLES FQQSFPDLQF PPLPERGDFD LREVLESPYF FN

References

[1]	"Six active phage-type RNA polymerase genes in Nicotiana tabacum." Hedtke B., Legen J., Weihe A., Herrmann R.G., Boerner T. Plant J. 30:625-637(2002) [PubMed: 12061895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ302018 mRNA. Translation: CAC82574.2. AJ416571 Genomic DNA. Translation: CAC95022.1.
3D structure databases
HSSP	HSSP built from PDB template 1ARO based on UniProtKB P00573.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.6. 298.
Family and domain databases
InterPro	IPR002092. DNA-dir_RNA_pol_phage. [Graphical view]
PANTHER	PTHR10102. RNA_pol_phage. 1 hit.
Pfam	PF00940. RNA_pol. 1 hit. [Graphical view]
PROSITE	PS00900. RNA_POL_PHAGE_1. 1 hit. PS00489. RNA_POL_PHAGE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPO1B_TOBAC

Accession

Primary (citable) accession number: Q8L6J5
Secondary accession number(s): Q8L6K2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)