Reviewed, UniProtKB/Swiss-Prot Q8L6J4 (RPO1A_TOBAC)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase 1A
    EC=2.7.7.6
Alternative name(s):
    T7 bacteriophage-type single subunit RNA polymerase 1A
    NictaRpoT1-syl

Gene names

Name:

RPOT1-SYL

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	155 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Belongs to the phage and mitochondrial RNA polymerase family.
Sequence caution	The sequence CAC95023.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 155	›155	DNA-directed RNA polymerase 1A		PRO_0000087751
Sites
	Active site	88	1	By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q8L6J4-1 [UniParc]. Last modified February 7, 2006. Version 2. Checksum: 854FC073023BE8BA	FASTA	155	17,959
	10 20 30 40 50 60 IIAMENHPVR WTTPLGLPVV QPYRKLGRHL IKTSLQILTL QRETDKVMVK RQRTAFPPNF 70 80 90 100 110 120 VHSLDGSHMM MTAIACKESG LSFAGVHDSY WTHASDVDQM NKILREKFVE LYDAPILENL 130 140 150 LESFQQSFPD LQFPPLPERG DFDLREVLES PYFFN

References

[1]	"Six active phage-type RNA polymerase genes in Nicotiana tabacum." Hedtke B., Legen J., Weihe A., Herrmann R.G., Boerner T. Plant J. 30:625-637(2002) [PubMed: 12061895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ416572 Genomic DNA. Translation: CAC95023.1. Sequence problems.
3D structure databases
HSSP	HSSP built from PDB template 1H38 based on UniProtKB P00573.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.6. 298.
Family and domain databases
InterPro	IPR002092. DNA-dir_RNA_pol_phage. [Graphical view]
PANTHER	PTHR10102. RNA_pol_phage. 1 hit.
Pfam	PF00940. RNA_pol. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPO1A_TOBAC

Accession

Primary (citable) accession number: Q8L6J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)