Q8L608 (HPSE2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heparanase-like protein 2
EC=3.2.-.-

Gene names

Ordered Locus Names:	At5g61250
ORF Names:	MFB13.2, MAF19.29

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans By similarity.
Subcellular location	Lysosome membrane; Peripheral membrane protein By similarity. Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 79 family.
Sequence caution	The sequence BAB08480.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Lysosome Membrane Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 539	518	Heparanase-like protein 2		PRO_0000042270
Sites
	Active site	198	1	Proton donor Potential
	Active site	316	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	143	1	N-linked (GlcNAc...) Potential
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
	Glycosylation	181	1	N-linked (GlcNAc...) Potential
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Glycosylation	421	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8L608 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A6D4B3D4A3627DA2	FASTA	539	59,865
	10 20 30 40 50 60 MGFNVVVFLS CLLLLPPVTF GSNMERTTLV IDGSRRIAET DENFICATLD WWPPEKCNYD 70 80 90 100 110 120 QCPWGYASLI NLNLASPLLA KAIQAFRTLR IRIGGSLQDQ VIYDVGDLKT PCTQFKKTDD 130 140 150 160 170 180 GLFGFSEGCL YMKRWDEVNH FFNATGAIVT FGLNALHGRN KLNGTAWGGD WDHTNTQDFM 190 200 210 220 230 240 NYTVSKGYAI DSWEFGNELS GSGIWASVSV ELYGKDLIVL KNVIKNVYKN SRTKPLVVAP 250 260 270 280 290 300 GGFFEEQWYS ELLRLSGPGV LDVLTHHIYN LGPGNDPKLV NKILDPNYLS GISELFANVN 310 320 330 340 350 360 QTIQEHGPWA AAWVGEAGGA FNSGGRQVSE TFINSFWYLD QLGISSKHNT KVYCRQALVG 370 380 390 400 410 420 GFYGLLEKET FVPNPDYYSA LLWHRLMGKG ILGVQTTASE YLRAYVHCSK RRAGITILLI 430 440 450 460 470 480 NLSKHTTFTV AVSNGVKVVL QAESMKRKSF LETIKSKVSW VGNKASDGYL NREEYHLSPK 490 500 510 520 530 DGDLRSKIML LNGKPLVPTA TGDIPKLEPV RHGVKSPVYI NPLSISFIVL PTFDAPACS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed: 9628582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB010073 Genomic DNA. Translation: BAB08480.1. Different initiation. CP002688 Genomic DNA. Translation: AED97442.1. CP002688 Genomic DNA. Translation: AED97443.1. AY099736 mRNA. Translation: AAM20587.1. BT002603 mRNA. Translation: AAO00963.1.
IPI	IPI00537091.
RefSeq	NP_200933.2. NM_125518.4. NP_851238.1. NM_180907.2.
UniGene	At.49202. At.60002.
3D structure databases
ProteinModelPortal	Q8L608.
ModBase	Search...
Protein family/group databases
CAZy	GH79. Glycoside Hydrolase Family 79.
Proteomic databases
PRIDE	Q8L608.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G61250.1; AT5G61250.1; AT5G61250. AT5G61250.2; AT5G61250.2; AT5G61250.
GeneID	836246.
GenomeReviews	Gene locus AT5G61250 in contig BA000015_GR.
KEGG	ath:AT5G61250.
NMPDR	fig|3702.1.peg.28144.
Organism-specific databases
TAIR	At5g61250.
Phylogenomic databases
eggNOG	euNOG05718.
GeneTree	EPGT00050000011306.
HOGENOM	HBG316724.
InParanoid	Q8L608.
OMA	GISSKHN.
PhylomeDB	Q8L608.
ProtClustDB	CLSN2690256.
Gene expression databases
ArrayExpress	Q8L608.
Genevestigator	Q8L608.
GermOnline	AT5G61250. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005199. Glyco_hydro_79. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR14363. Glyco_hydro_79_N. 1 hit.
Pfam	PF03662. Glyco_hydro_79n. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPSE2_ARATH

Accession

Primary (citable) accession number: Q8L608
Secondary accession number(s): Q9FLK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 1, 2002
Last modified:	September 21, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program