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Protein

Pantothenate kinase 2

Gene

PANK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of pantothenate the first step in CoA biosynthesis. May play a role in the physiological regulation of the intracellular CoA concentration. Functionally redudant with PANK1.1 Publication

Catalytic activityi

ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate.

Enzyme regulationi

Regulated by feedback inhibition by malonyl-CoA.By similarity

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CoA from (R)-pantothenate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pantothenate kinase 2 (PANK2), Pantothenate kinase 1 (PANK1)
  2. Phosphopantothenate--cysteine ligase 2 (PPCS2), Phosphopantothenate--cysteine ligase 1 (PPCS1)
  3. Probable phosphopantothenoylcysteine decarboxylase (HAL3B), Phosphopantothenoylcysteine decarboxylase (HAL3A)
  4. Phosphopantetheine adenylyltransferase (COAD)
  5. Dephospho-CoA kinase (COAE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • pantothenate kinase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Coenzyme A biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G32180-MONOMER.
ARA:GQT-2302-MONOMER.
ARA:GQT-2303-MONOMER.
MetaCyc:AT4G32180-MONOMER.
UniPathwayiUPA00241; UER00352.

Names & Taxonomyi

Protein namesi
Recommended name:
Pantothenate kinase 2 (EC:2.7.1.33)
Short name:
AtPANK2
Alternative name(s):
Pantothenic acid kinase 2
Gene namesi
Name:PANK2
Ordered Locus Names:At4g32180
ORF Names:F10M6.180, F10N7.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G32180.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but homozygous double mutants pank1-1 and pank2-1 are embryonic lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 901901Pantothenate kinase 2PRO_0000161809Add
BLAST

Proteomic databases

PaxDbiQ8L5Y9.
PRIDEiQ8L5Y9.

PTM databases

iPTMnetiQ8L5Y9.

Expressioni

Tissue specificityi

Highly expressed in leaves and developing seeds. Expressed in roots, stems and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ8L5Y9. baseline and differential.
GenevisibleiQ8L5Y9. AT.

Interactioni

Protein-protein interaction databases

BioGridi14637. 3 interactions.
IntActiQ8L5Y9. 1 interaction.
MINTiMINT-8070348.
STRINGi3702.AT4G32180.1.

Structurei

3D structure databases

ProteinModelPortaliQ8L5Y9.
SMRiQ8L5Y9. Positions 80-426, 530-896.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the type II pantothenate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2201. Eukaryota.
KOG4584. Eukaryota.
COG5146. LUCA.
HOGENOMiHOG000194691.
InParanoidiQ8L5Y9.
OMAiPPYEISV.

Family and domain databases

InterProiIPR002791. DUF89.
IPR015844. PanK_long.
IPR004567. Type_II_PanK.
[Graphical view]
PfamiPF01937. DUF89. 1 hit.
PF03630. Fumble. 1 hit.
[Graphical view]
PIRSFiPIRSF036939. PanK_long. 1 hit.
SUPFAMiSSF111321. SSF111321. 1 hit.
TIGRFAMsiTIGR00555. panK_eukar. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8L5Y9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGQEDEYDP ILDNKREAEA KSQVSVAADK NMAPSTSGTP IHRSGSRPQL
60 70 80 90 100
DLSKAEIQGN LEERDPTILL PNQSDDISHL ALDIGGSLIK LLYFSRHEDY
110 120 130 140 150
SNDDDKRKRT IKERLGITNG NLRSYPVLGG RLHFVKFETH KINECLDFIH
160 170 180 190 200
SKQLHRRDPY PWSSKTLPLG TGVIKVTGGG AFKFADLFKE RLGVSIEKED
210 220 230 240 250
EMHCLVSGAN FLLKAIRHEA FTHMEGEKEF VQIDPNDLYP YLLVNVGSGV
260 270 280 290 300
SIIKVDGEGK FERVSGTNVG GGTYWGLGRL LTKCKSFDEL LELSQKGDNS
310 320 330 340 350
AIDMLVGDIY GGMDYSKIGL SASTIASSFG KAISENKELD DYRPEDISLS
360 370 380 390 400
LLRMISYNIG QISYLNALRF GLKRIFFGGF FIRGHAYTMD TISFAVHFWS
410 420 430 440 450
KGEMQAMFLR HEGFLGALGA FMSYEKHGLD DLMSHQLVER FPMGAPYTGG
460 470 480 490 500
NIHGPPLGDL DEKISWMEKF VRRGTEITAP VPMTPSKTTG LGGFEVPSSR
510 520 530 540 550
GSALRSDASA LNVGVLHLVP TLEVFPLLAD PKTYEPNTID LSDQGEREYW
560 570 580 590 600
LKVLSEHLPD LVDTAVASEG GTEDAKRRGD AFARAFSAHL ARLMEEPAAY
610 620 630 640 650
GKLGLANLLE LREECLREFQ FVDAYRSIKQ RENEASLAVL PDLLEELDSM
660 670 680 690 700
SEEARLLTLI EGVLAANIFD WGSRACVDLY HKGTIIEIYR MSRNKMQRPW
710 720 730 740 750
RVDDFDAFKE RMLGSGGKQP HRHKRALLFV DNSGADVILG MLPLAREFLR
760 770 780 790 800
RGTEVVLVAN SLPALNDVTA MELPDIVAGA AKHCDILRRA AEMGGLLVDA
810 820 830 840 850
MVNPGDGSKK DSTSAPLMVV ENGCGSPCID LRQVSSELAA AAKDADLVVL
860 870 880 890 900
EGMGRALHTN FNAQFQCEAL KLAMVKNQRL AEKLIKGNIY DCVCRYEPPS

L
Length:901
Mass (Da):99,640
Last modified:May 31, 2011 - v2
Checksum:iBF5176A4B06B8108
GO

Sequence cautioni

The sequence CAA16571.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA16972.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB79936.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021636 Genomic DNA. Translation: CAA16571.1. Sequence problems.
AL021811 Genomic DNA. Translation: CAA16972.1. Sequence problems.
AL161580 Genomic DNA. Translation: CAB79936.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86015.1.
AY099839 mRNA. Translation: AAM20690.1.
RefSeqiNP_194945.3. NM_119370.4. [Q8L5Y9-1]
UniGeneiAt.28173.

Genome annotation databases

EnsemblPlantsiAT4G32180.1; AT4G32180.1; AT4G32180. [Q8L5Y9-1]
GeneIDi829351.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021636 Genomic DNA. Translation: CAA16571.1. Sequence problems.
AL021811 Genomic DNA. Translation: CAA16972.1. Sequence problems.
AL161580 Genomic DNA. Translation: CAB79936.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86015.1.
AY099839 mRNA. Translation: AAM20690.1.
RefSeqiNP_194945.3. NM_119370.4. [Q8L5Y9-1]
UniGeneiAt.28173.

3D structure databases

ProteinModelPortaliQ8L5Y9.
SMRiQ8L5Y9. Positions 80-426, 530-896.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14637. 3 interactions.
IntActiQ8L5Y9. 1 interaction.
MINTiMINT-8070348.
STRINGi3702.AT4G32180.1.

PTM databases

iPTMnetiQ8L5Y9.

Proteomic databases

PaxDbiQ8L5Y9.
PRIDEiQ8L5Y9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G32180.1; AT4G32180.1; AT4G32180. [Q8L5Y9-1]
GeneIDi829351.

Organism-specific databases

TAIRiAT4G32180.

Phylogenomic databases

eggNOGiKOG2201. Eukaryota.
KOG4584. Eukaryota.
COG5146. LUCA.
HOGENOMiHOG000194691.
InParanoidiQ8L5Y9.
OMAiPPYEISV.

Enzyme and pathway databases

UniPathwayiUPA00241; UER00352.
BioCyciARA:AT4G32180-MONOMER.
ARA:GQT-2302-MONOMER.
ARA:GQT-2303-MONOMER.
MetaCyc:AT4G32180-MONOMER.

Miscellaneous databases

PROiQ8L5Y9.

Gene expression databases

ExpressionAtlasiQ8L5Y9. baseline and differential.
GenevisibleiQ8L5Y9. AT.

Family and domain databases

InterProiIPR002791. DUF89.
IPR015844. PanK_long.
IPR004567. Type_II_PanK.
[Graphical view]
PfamiPF01937. DUF89. 1 hit.
PF03630. Fumble. 1 hit.
[Graphical view]
PIRSFiPIRSF036939. PanK_long. 1 hit.
SUPFAMiSSF111321. SSF111321. 1 hit.
TIGRFAMsiTIGR00555. panK_eukar. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 26-901.
    Strain: cv. Columbia.
  4. "Plant coenzyme A biosynthesis: characterization of two pantothenate kinases from Arabidopsis."
    Tilton G.B., Wedemeyer W.J., Browse J., Ohlrogge J.
    Plant Mol. Biol. 61:629-642(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  5. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPANK2_ARATH
AccessioniPrimary (citable) accession number: Q8L5Y9
Secondary accession number(s): O49372, O49374
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: May 31, 2011
Last modified: January 20, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.