Q8L5Y6 (CAND1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cullin-associated NEDD8-dissociated protein 1

Alternative name(s):
Cullin-associated and neddylation-dissociated protein 1
Short name=AtCAND1
Protein ENHANCER OF TIR1-1 AUXIN RESISTANCE 2
Protein HEMIVENATA

Gene names

Name:	CAND1
Synonyms:	ETA2, HVE
Ordered Locus Names:	At2g02560
ORF Names:	T8K22.14

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1219 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for SCF(TIR1) function. Modulates SCF(TIR1) function through its interactions with the CUL1 subunit. Positively regulates multiple E3 ubiquitin-protein ligase complexes and their associated developmental processes. Represses photomorphogenesis by promoting HY5 degradation in darkness. Ref.4 Ref.5 Ref.6 Ref.7 Ref.9
Subunit structure	Interacts with CUL1 and CUL4. Binds unneddylated CUL1, but cannot bind CUL1 once it has been neddylated. Ref.4 Ref.8 Ref.9
Tissue specificity	Highly expressed in roots. Expressed in stems, flowers and siliques. Ref.5
Disruption phenotype	Developmental phenotypes such as dwarfism, organ defects, short inflorescences and mishaped leaves. Low fertility and reduced responses to hormones. Ref.4 Ref.6 Ref.7
Sequence similarities	Belongs to the CAND family. Contains 18 HEAT repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to auxin stimulus Inferred from mutant phenotype Ref.6. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW vegetative to reproductive phase transition of meristem Inferred from mutant phenotype Ref.4. Source: TAIR xylem and phloem pattern formation Inferred from mutant phenotype Ref.7. Source: TAIR
Cellular component	cell wall Inferred from direct assay. Source: TAIR cytosol Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	protein binding Inferred from physical interaction Ref.8Ref.9. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CUL1	Q94AH6	8	EBI-602912,EBI-532411
CUL4	Q8LGH4	2	EBI-602912,EBI-541750

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8L5Y6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8L5Y6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     13-14: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1219	1219	Cullin-associated NEDD8-dissociated protein 1		PRO_0000396859
Regions
	Repeat	44 – 81	38	HEAT 1
	Repeat	83 – 119	37	HEAT 2
	Repeat	209 – 244	36	HEAT 3
	Repeat	248 – 288	41	HEAT 4
	Repeat	327 – 363	37	HEAT 5
	Repeat	367 – 404	38	HEAT 6
	Repeat	423 – 460	38	HEAT 7
	Repeat	464 – 503	40	HEAT 8
	Repeat	599 – 636	38	HEAT 9
	Repeat	639 – 676	38	HEAT 10
	Repeat	808 – 848	41	HEAT 11
	Repeat	850 – 883	34	HEAT 12
	Repeat	927 – 964	38	HEAT 13
	Repeat	966 – 998	33	HEAT 14
	Repeat	1002 – 1039	38	HEAT 15
	Repeat	1043 – 1079	37	HEAT 16
	Repeat	1101 – 1137	37	HEAT 17
	Repeat	1141 – 1180	40	HEAT 18
Natural variations
	Alternative sequence	13 – 14	2	Missing in isoform 2.		VSP_039624
Experimental info
	Mutagenesis	1069	1	G → D in eta2-1; reduced response to auxin. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: B0F5769754DE6040	FASTA	1,219	134,897
	10 20 30 40 50 60 MANLQVSGIL EKFQMTGKDK DYRYMATSDL LNELNKDSFK IDLDLEVRLS SIILQQLDDV 70 80 90 100 110 120 AGDVSGLAVK CLAPLVKKVG EERIVEMTNK LCDKLLHGKD QHRDTASIAL RTVVAQIAPT 130 140 150 160 170 180 LAPSILVTLT PQMIGGISGQ GMSSGIKCEC LEIMCDVVQK YGSLMTDDHE KLLNTLLLQL 190 200 210 220 230 240 GCNQATVRKK TVTCIASLAS SLSDDLLAKA TVEVVKNLSN RNAKSEITRT NIQMIGALCR 250 260 270 280 290 300 AVGYRFGTHL GNTVPVLINY CTSASENDEE LREYSLQALE SFLLRCPRDI SPYCDEILNL 310 320 330 340 350 360 TLEYISYDPN FTDNMEEDTD NETLEDEEDD ESANEYTDDE DASWKVRRAA AKCLAGLIVS 370 380 390 400 410 420 RSEMLTKVYQ EACPKLIDRF KEREENVKMD VFNTFIDLLR QTGNVTKGQT DTDESSPKWL 430 440 450 460 470 480 LKQEVSKIVK SINRQLREKS VKTKVGAFSV LRELVVVLPD CLADHIGSLV PGIERALNDK 490 500 510 520 530 540 SSTSNLKIEA LVFTKLVLAS HAPPVFHPYI KALSSPVLAA VGERYYKVTA EALRVCGELV 550 560 570 580 590 600 RVVRPSTAGM GFDFKPFVHP IYNAIMSRLT NQDQDQEVKE CAITCMGLVI STFGDQLRAE 610 620 630 640 650 660 LPSCLPVLVD RMGNEITRLT AVKAFSVIAT SPLHINLSCV LDHLIAELTG FLRKANRVLR 670 680 690 700 710 720 QATLITMNTL VTAYGDKIGS EAYEVILVEL SSLISVSDLH MTALALELCC TLMTGKSCSE 730 740 750 760 770 780 NISLAVRNKV LPQALTLVKS PLLQGQALLD LQKFFEALVY HANTSFYTLL ESLLSCAKPS 790 800 810 820 830 840 PQSGGVPKQA LYSIAQCVAV LCLAAGDKNC SSTVKMLMEI LKDDSGTNSA KQHLALLSLG 850 860 870 880 890 900 EIGRRKDLSA HAGIETIVIE SFQSPFEEIK SAASYALGNI AVGNLSNYLP FILDQIDNQQ 910 920 930 940 950 960 KKQYILLHSL KEVIVRQSVD KADFQNSSVE KILALLFNHC ESEEEGVRNV VAECLGKMAL 970 980 990 1000 1010 1020 IEPEKLVPAL QVRTTSPAAF TRATVVTAVK YSVVERPEKL DEIIFPQISS FLMLIKDGDR 1030 1040 1050 1060 1070 1080 HVRRAAVSAL STFAHYKPNL IKGLLPELLP LLYDQTVIKK ELIRTVDLGP FKHVVDDGLE 1090 1100 1110 1120 1130 1140 LRKAAFECVF TLVDSCLDQV NPSSFIVPFL KSGLEDHYDL KMLCHLILSL LADKCPSAVL 1150 1160 1170 1180 1190 1200 AVLDSLVEPL HKTISFKPKQ DAVKQEHDRN EDMIRSALRA ISSLDRINGV DYSHKFKGLM 1210 GDMKRSVPLW EKFQTIRNE
	Isoform 2 [UniParc]. Checksum: AA23DAE4A94EE1EA	FASTA	1,217	134,622
	10 20 30 40 50 60 MANLQVSGIL EKMTGKDKDY RYMATSDLLN ELNKDSFKID LDLEVRLSSI ILQQLDDVAG 70 80 90 100 110 120 DVSGLAVKCL APLVKKVGEE RIVEMTNKLC DKLLHGKDQH RDTASIALRT VVAQIAPTLA 130 140 150 160 170 180 PSILVTLTPQ MIGGISGQGM SSGIKCECLE IMCDVVQKYG SLMTDDHEKL LNTLLLQLGC 190 200 210 220 230 240 NQATVRKKTV TCIASLASSL SDDLLAKATV EVVKNLSNRN AKSEITRTNI QMIGALCRAV 250 260 270 280 290 300 GYRFGTHLGN TVPVLINYCT SASENDEELR EYSLQALESF LLRCPRDISP YCDEILNLTL 310 320 330 340 350 360 EYISYDPNFT DNMEEDTDNE TLEDEEDDES ANEYTDDEDA SWKVRRAAAK CLAGLIVSRS 370 380 390 400 410 420 EMLTKVYQEA CPKLIDRFKE REENVKMDVF NTFIDLLRQT GNVTKGQTDT DESSPKWLLK 430 440 450 460 470 480 QEVSKIVKSI NRQLREKSVK TKVGAFSVLR ELVVVLPDCL ADHIGSLVPG IERALNDKSS 490 500 510 520 530 540 TSNLKIEALV FTKLVLASHA PPVFHPYIKA LSSPVLAAVG ERYYKVTAEA LRVCGELVRV 550 560 570 580 590 600 VRPSTAGMGF DFKPFVHPIY NAIMSRLTNQ DQDQEVKECA ITCMGLVIST FGDQLRAELP 610 620 630 640 650 660 SCLPVLVDRM GNEITRLTAV KAFSVIATSP LHINLSCVLD HLIAELTGFL RKANRVLRQA 670 680 690 700 710 720 TLITMNTLVT AYGDKIGSEA YEVILVELSS LISVSDLHMT ALALELCCTL MTGKSCSENI 730 740 750 760 770 780 SLAVRNKVLP QALTLVKSPL LQGQALLDLQ KFFEALVYHA NTSFYTLLES LLSCAKPSPQ 790 800 810 820 830 840 SGGVPKQALY SIAQCVAVLC LAAGDKNCSS TVKMLMEILK DDSGTNSAKQ HLALLSLGEI 850 860 870 880 890 900 GRRKDLSAHA GIETIVIESF QSPFEEIKSA ASYALGNIAV GNLSNYLPFI LDQIDNQQKK 910 920 930 940 950 960 QYILLHSLKE VIVRQSVDKA DFQNSSVEKI LALLFNHCES EEEGVRNVVA ECLGKMALIE 970 980 990 1000 1010 1020 PEKLVPALQV RTTSPAAFTR ATVVTAVKYS VVERPEKLDE IIFPQISSFL MLIKDGDRHV 1030 1040 1050 1060 1070 1080 RRAAVSALST FAHYKPNLIK GLLPELLPLL YDQTVIKKEL IRTVDLGPFK HVVDDGLELR 1090 1100 1110 1120 1130 1140 KAAFECVFTL VDSCLDQVNP SSFIVPFLKS GLEDHYDLKM LCHLILSLLA DKCPSAVLAV 1150 1160 1170 1180 1190 1200 LDSLVEPLHK TISFKPKQDA VKQEHDRNED MIRSALRAIS SLDRINGVDY SHKFKGLMGD 1210 MKRSVPLWEK FQTIRNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation." Feng S., Shen Y., Sullivan J.A., Rubio V., Xiong Y., Sun T.P., Deng X.W. Plant Cell 16:1870-1882(2004) [PubMed: 15208391] [Abstract] Cited for: FUNCTION, INTERACTION WITH CUL1, DISRUPTION PHENOTYPE.
[5]	"Arabidopsis ETA2, an apparent ortholog of the human cullin-interacting protein CAND1, is required for auxin responses mediated by the SCF(TIR1) ubiquitin ligase." Chuang H.W., Zhang W., Gray W.M. Plant Cell 16:1883-1897(2004) [PubMed: 15208392] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF GLY-1069.
[6]	"AtCAND1, a HEAT-repeat protein that participates in auxin signaling in Arabidopsis." Cheng Y., Dai X., Zhao Y. Plant Physiol. 135:1020-1026(2004) [PubMed: 15181201] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis." Alonso-Peral M.M., Candela H., del Pozo J.C., Martinez-Laborda A., Ponce M.R., Micol J.L. Development 133:3755-3766(2006) [PubMed: 16943276] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]	"Arabidopsis CULLIN4 forms an E3 ubiquitin ligase with RBX1 and the CDD complex in mediating light control of development." Chen H., Shen Y., Tang X., Yu L., Wang J., Guo L., Zhang Y., Zhang H., Feng S., Strickland E., Zheng N., Deng X.-W. Plant Cell 18:1991-2004(2006) [PubMed: 16844902] [Abstract] Cited for: INTERACTION WITH CUL4.
[9]	"Genetic analysis of CAND1-CUL1 interactions in Arabidopsis supports a role for CAND1-mediated cycling of the SCFTIR1 complex." Zhang W., Ito H., Quint M., Huang H., Noel L.D., Gray W.M. Proc. Natl. Acad. Sci. U.S.A. 105:8470-8475(2008) [PubMed: 18550827] [Abstract] Cited for: FUNCTION, INTERACTION WITH CUL1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004136 Genomic DNA. Translation: AAC18930.1. CP002685 Genomic DNA. Translation: AEC05595.1. CP002685 Genomic DNA. Translation: AEC05596.1. AY099857 mRNA. Translation: AAM20708.1. BT010134 mRNA. Translation: AAQ22603.1.
IPI	IPI00524474. IPI00656867.
PIR	T00607.
RefSeq	NP_001030954.1. NM_001035877.1. NP_178360.2. NM_126312.2.
UniGene	At.41482.
3D structure databases
ProteinModelPortal	Q8L5Y6.
SMR	Q8L5Y6. Positions 45-78, 172-200, 233-286, 343-399, 644-675, 858-884, 940-1036.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-33719N.
IntAct	Q8L5Y6. 12 interactions.
STRING	Q8L5Y6.
Proteomic databases
PRIDE	Q8L5Y6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G02560.1; AT2G02560.1; AT2G02560.
GeneID	814786.
GenomeReviews	Gene locus AT2G02560 in contig CT485783_GR.
KEGG	ath:AT2G02560.
NMPDR	fig|3702.1.peg.7850.
Organism-specific databases
TAIR	At2g02560.
Phylogenomic databases
GeneTree	EPGT00050000011488.
HOGENOM	HBG560547.
InParanoid	Q8L5Y6.
OMA	EGSRNVV.
PhylomeDB	Q8L5Y6.
ProtClustDB	CLSN2690104.
Gene expression databases
Genevestigator	Q8L5Y6.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR013932. TATA-bd_TIP120. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 2 hits.
Pfam	PF08623. TIP120. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50077. HEAT_REPEAT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAND1_ARATH

Accession

Primary (citable) accession number: Q8L5Y6
Secondary accession number(s): O64720

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program