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Protein

COP9 signalosome complex subunit 4

Gene

CSN4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. It is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of IAA6 by regulating the activity of the Ubl ligase SCF-TIR complex.2 Publications

GO - Biological processi

  • cullin deneddylation Source: TAIR
  • multicellular organism development Source: UniProtKB-KW
  • positive regulation of G2/M transition of mitotic cell cycle Source: TAIR
  • red, far-red light phototransduction Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Phytochrome signaling pathway

Enzyme and pathway databases

ReactomeiR-ATH-5696394. DNA Damage Recognition in GG-NER.
R-ATH-6781823. Formation of TC-NER Pre-Incision Complex.

Names & Taxonomyi

Protein namesi
Recommended name:
COP9 signalosome complex subunit 4
Short name:
AtS4
Short name:
Signalosome subunit 4
Alternative name(s):
Constitutive photomorphogenesis protein 8
Protein FUSCA 4
Gene namesi
Name:CSN4
Synonyms:COP8, FUS4
Ordered Locus Names:At5g42970
ORF Names:MBD2.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G42970.

Subcellular locationi

GO - Cellular componenti

  • COP9 signalosome Source: TAIR
  • cytosol Source: TAIR
  • protein complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signalosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi251 – 26515Missing in fu4-414; induces seedlings defects and lethality after the seedling stage. Add
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397COP9 signalosome complex subunit 4PRO_0000120993Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ8L5U0.
PRIDEiQ8L5U0.

PTM databases

iPTMnetiQ8L5U0.

Expressioni

Gene expression databases

ExpressionAtlasiQ8L5U0. baseline and differential.
GenevisibleiQ8L5U0. AT.

Interactioni

Subunit structurei

Component of the CSN complex, probably composed of CSN1, CSN2, CSN3, CSN4, CSN5 (CSN5A or CSN5B), CSN6 (CSN6A or CSN6B), CSN7 and CSN8. Interacts with itself. In the complex, it is located in the center and probably interacts directly with CSN1, CSN2, CSN3, CSN4, CSN5A or CSN5B, CSN6A or CSN6B, CSN7 and CSN8. Interacts with COP10. Binds to the translation initiation factors TIF3E1 (PubMed:19704582).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CSN1P454323EBI-531074,EBI-530996
CSN3Q8W5755EBI-531074,EBI-531055
CSN7Q94JU35EBI-531074,EBI-531152
CSN8P432553EBI-531074,EBI-530981
CUL4Q8LGH42EBI-531074,EBI-541750

Protein-protein interaction databases

BioGridi19562. 19 interactions.
IntActiQ8L5U0. 12 interactions.
MINTiMINT-8071037.
STRINGi3702.AT5G42970.1.

Structurei

3D structure databases

ProteinModelPortaliQ8L5U0.
SMRiQ8L5U0. Positions 175-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini190 – 362173PCIAdd
BLAST

Sequence similaritiesi

Belongs to the CSN4 family.Curated
Contains 1 PCI domain.Curated

Phylogenomic databases

eggNOGiKOG1497. Eukaryota.
ENOG410XPDE. LUCA.
HOGENOMiHOG000158382.
InParanoidiQ8L5U0.
KOiK12178.
OMAiLEDNDSM.
OrthoDBiEOG09360C28.
PhylomeDBiQ8L5U0.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000717. PCI_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01399. PCI. 1 hit.
[Graphical view]
SMARTiSM00088. PINT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8L5U0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEALTNASA IGDQRQKIEQ YKLILSSVLS SNDLLQAQRF IDHILSDDVP
60 70 80 90 100
LVVSRQLLQS FAQELGRLEP ETQKEIAQFT LTQIQPRVVS FEEQALVIRE
110 120 130 140 150
KLAGLYESEQ EWSKAAQMLS GIDLDSGMRA VDDNFKLSKC IQIARLYLED
160 170 180 190 200
DDAVNAEAFI NKASFLVSNS QNEVLNLQYK VCYARILDMK RKFLEAALRY
210 220 230 240 250
YGISQIEQRQ IGDEEIDENA LEQALSAAVT CTILAGAGPQ RSRVLATLYK
260 270 280 290 300
DERCSKLKIY PILQKVYLER ILRRPEIDAF SEELRPHQKA SLPDKSTVLD
310 320 330 340 350
RAMIEHNLLS ASKLYTNIRF DELGTLLAID PRKAEKIAAN MIGQDRMRGS
360 370 380 390
IDQEEAVIHF EDDVEELQQW DQQISGLCQA LNDILDGMAK KGMSVPV
Length:397
Mass (Da):44,957
Last modified:November 23, 2004 - v2
Checksum:i1E8AEF28C35B28F0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti244 – 2441V → F in AAM64629 (Ref. 5) Curated
Sequence conflicti303 – 3031M → V in AAD51742 (PubMed:10521526).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176089 mRNA. Translation: AAD51742.1.
AF395060 mRNA. Translation: AAL58103.1.
AB008264 Genomic DNA. Translation: BAB09199.1.
CP002688 Genomic DNA. Translation: AED94896.1.
AY087068 mRNA. Translation: AAM64629.1.
AK176360 mRNA. Translation: BAD44123.1.
PIRiT52302.
RefSeqiNP_199111.1. NM_123663.2.
UniGeneiAt.23570.

Genome annotation databases

EnsemblPlantsiAT5G42970.1; AT5G42970.1; AT5G42970.
GeneIDi834312.
GrameneiAT5G42970.1; AT5G42970.1; AT5G42970.
KEGGiath:AT5G42970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176089 mRNA. Translation: AAD51742.1.
AF395060 mRNA. Translation: AAL58103.1.
AB008264 Genomic DNA. Translation: BAB09199.1.
CP002688 Genomic DNA. Translation: AED94896.1.
AY087068 mRNA. Translation: AAM64629.1.
AK176360 mRNA. Translation: BAD44123.1.
PIRiT52302.
RefSeqiNP_199111.1. NM_123663.2.
UniGeneiAt.23570.

3D structure databases

ProteinModelPortaliQ8L5U0.
SMRiQ8L5U0. Positions 175-390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19562. 19 interactions.
IntActiQ8L5U0. 12 interactions.
MINTiMINT-8071037.
STRINGi3702.AT5G42970.1.

PTM databases

iPTMnetiQ8L5U0.

Proteomic databases

PaxDbiQ8L5U0.
PRIDEiQ8L5U0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G42970.1; AT5G42970.1; AT5G42970.
GeneIDi834312.
GrameneiAT5G42970.1; AT5G42970.1; AT5G42970.
KEGGiath:AT5G42970.

Organism-specific databases

TAIRiAT5G42970.

Phylogenomic databases

eggNOGiKOG1497. Eukaryota.
ENOG410XPDE. LUCA.
HOGENOMiHOG000158382.
InParanoidiQ8L5U0.
KOiK12178.
OMAiLEDNDSM.
OrthoDBiEOG09360C28.
PhylomeDBiQ8L5U0.

Enzyme and pathway databases

ReactomeiR-ATH-5696394. DNA Damage Recognition in GG-NER.
R-ATH-6781823. Formation of TC-NER Pre-Incision Complex.

Miscellaneous databases

PROiQ8L5U0.

Gene expression databases

ExpressionAtlasiQ8L5U0. baseline and differential.
GenevisibleiQ8L5U0. AT.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000717. PCI_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01399. PCI. 1 hit.
[Graphical view]
SMARTiSM00088. PINT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCSN4_ARATH
AccessioniPrimary (citable) accession number: Q8L5U0
Secondary accession number(s): Q9FMM1, Q9SW74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.