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Protein

COP9 signalosome complex subunit 4

Gene

CSN4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. It is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of IAA6 by regulating the activity of the Ubl ligase SCF-TIR complex.2 Publications

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • positive regulation of G2/M transition of mitotic cell cycle Source: TAIR
  • protein deneddylation Source: TAIR
  • red, far-red light phototransduction Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein
Biological processPhytochrome signaling pathway

Enzyme and pathway databases

ReactomeiR-ATH-5696394 DNA Damage Recognition in GG-NER
R-ATH-6781823 Formation of TC-NER Pre-Incision Complex

Names & Taxonomyi

Protein namesi
Recommended name:
COP9 signalosome complex subunit 4
Short name:
AtS4
Short name:
Signalosome subunit 4
Alternative name(s):
Constitutive photomorphogenesis protein 8
Protein FUSCA 4
Gene namesi
Name:CSN4
Synonyms:COP8, FUS4
Ordered Locus Names:At5g42970
ORF Names:MBD2.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G42970
TAIRilocus:2159961 AT5G42970

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signalosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi251 – 265Missing in fu4-414; induces seedlings defects and lethality after the seedling stage. Add BLAST15

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001209931 – 397COP9 signalosome complex subunit 4Add BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ8L5U0
PRIDEiQ8L5U0

PTM databases

iPTMnetiQ8L5U0

Expressioni

Gene expression databases

ExpressionAtlasiQ8L5U0 baseline and differential
GenevisibleiQ8L5U0 AT

Interactioni

Subunit structurei

Component of the CSN complex, probably composed of CSN1, CSN2, CSN3, CSN4, CSN5 (CSN5A or CSN5B), CSN6 (CSN6A or CSN6B), CSN7 and CSN8. Interacts with itself. In the complex, it is located in the center and probably interacts directly with CSN1, CSN2, CSN3, CSN4, CSN5A or CSN5B, CSN6A or CSN6B, CSN7 and CSN8. Interacts with COP10. Binds to the translation initiation factors TIF3E1 (PubMed:19704582).5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi19562, 19 interactors
IntActiQ8L5U0, 12 interactors
STRINGi3702.AT5G42970.1

Structurei

3D structure databases

ProteinModelPortaliQ8L5U0
SMRiQ8L5U0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini197 – 365PCIPROSITE-ProRule annotationAdd BLAST169

Sequence similaritiesi

Belongs to the CSN4 family.Curated

Phylogenomic databases

eggNOGiKOG1497 Eukaryota
ENOG410XPDE LUCA
HOGENOMiHOG000158382
InParanoidiQ8L5U0
KOiK12178
OMAiYNNITFR
OrthoDBiEOG09360C28
PhylomeDBiQ8L5U0

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR037754 CSN4
IPR000717 PCI_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10855:SF2 PTHR10855:SF2, 1 hit
PfamiView protein in Pfam
PF01399 PCI, 1 hit
SMARTiView protein in SMART
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequencei

Sequence statusi: Complete.

Q8L5U0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEALTNASA IGDQRQKIEQ YKLILSSVLS SNDLLQAQRF IDHILSDDVP
60 70 80 90 100
LVVSRQLLQS FAQELGRLEP ETQKEIAQFT LTQIQPRVVS FEEQALVIRE
110 120 130 140 150
KLAGLYESEQ EWSKAAQMLS GIDLDSGMRA VDDNFKLSKC IQIARLYLED
160 170 180 190 200
DDAVNAEAFI NKASFLVSNS QNEVLNLQYK VCYARILDMK RKFLEAALRY
210 220 230 240 250
YGISQIEQRQ IGDEEIDENA LEQALSAAVT CTILAGAGPQ RSRVLATLYK
260 270 280 290 300
DERCSKLKIY PILQKVYLER ILRRPEIDAF SEELRPHQKA SLPDKSTVLD
310 320 330 340 350
RAMIEHNLLS ASKLYTNIRF DELGTLLAID PRKAEKIAAN MIGQDRMRGS
360 370 380 390
IDQEEAVIHF EDDVEELQQW DQQISGLCQA LNDILDGMAK KGMSVPV
Length:397
Mass (Da):44,957
Last modified:November 23, 2004 - v2
Checksum:i1E8AEF28C35B28F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti244V → F in AAM64629 (Ref. 5) Curated1
Sequence conflicti303M → V in AAD51742 (PubMed:10521526).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176089 mRNA Translation: AAD51742.1
AF395060 mRNA Translation: AAL58103.1
AB008264 Genomic DNA Translation: BAB09199.1
CP002688 Genomic DNA Translation: AED94896.1
AY087068 mRNA Translation: AAM64629.1
AK176360 mRNA Translation: BAD44123.1
PIRiT52302
RefSeqiNP_199111.1, NM_123663.3
UniGeneiAt.23570

Genome annotation databases

EnsemblPlantsiAT5G42970.1; AT5G42970.1; AT5G42970
GeneIDi834312
GrameneiAT5G42970.1; AT5G42970.1; AT5G42970
KEGGiath:AT5G42970

Similar proteinsi

Entry informationi

Entry nameiCSN4_ARATH
AccessioniPrimary (citable) accession number: Q8L5U0
Secondary accession number(s): Q9FMM1, Q9SW74
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: May 23, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health