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Protein

Xylanase inhibitor protein 1

Gene

XIPI

Organism
Triticum aestivum (Wheat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Fungal xylanase inhibitor. Possesses competitive inhibiting activity against fungal endo-1,4-beta-D-xylanases belonging to glycoside hydrolase family 10 (GH10) and family 11 (GH11). Possesses also inhibitory activity towards barley alpha-amylases. Binding to xylanases or amylases is necessary for inhibition activity. May function in plant defense against secreted fungal pathogen xylanases. Is similar to class III chitinases, but does not exhibit chitinase activity.4 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Plant defense

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Xylanase inhibitor protein 1
Short name:
XIP-1
Short name:
XIP-I
Alternative name(s):
Class III chitinase homolog
Gene namesi
Name:XIPI
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeTriticinaeTriticum
Proteomesi
  • UP000019116 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei9596. Tri a XI.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 302 PublicationsAdd BLAST30
ChainiPRO_000001199031 – 304Xylanase inhibitor protein 1Add BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi55 ↔ 96
Glycosylationi119N-linked (GlcNAc...)1
Disulfide bondi194 ↔ 225
Glycosylationi295N-linked (GlcNAc...)1

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Developmental stagei

Expressed in immature embryos 3 weeks after pollination, in roots and shoots of 3 day and 5 day old seedlings, and in roots of 10 day old seedlings.1 Publication

Inductioni

By wounding, methyl jasmonate, and by E.graminis infection in leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ8L5C6. baseline and differential.
GenevisibleiQ8L5C6. TA.

Interactioni

Subunit structurei

Binds to fungal GH10 and GH11 xylanases. Forms also a ternary complex with barley alpha-amylase 1 (AMY1) and insoluble starch.1 Publication

Structurei

Secondary structure

1304
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi38 – 44Combined sources7
Helixi46 – 48Combined sources3
Helixi51 – 56Combined sources6
Beta strandi61 – 75Combined sources15
Helixi86 – 88Combined sources3
Helixi89 – 98Combined sources10
Beta strandi103 – 112Combined sources10
Helixi119 – 133Combined sources15
Turni144 – 147Combined sources4
Beta strandi151 – 159Combined sources9
Helixi166 – 174Combined sources9
Beta strandi179 – 183Combined sources5
Beta strandi187 – 192Combined sources6
Beta strandi194 – 197Combined sources4
Helixi200 – 206Combined sources7
Beta strandi213 – 217Combined sources5
Turni227 – 229Combined sources3
Helixi231 – 241Combined sources11
Beta strandi245 – 252Combined sources8
Helixi263 – 268Combined sources6
Helixi270 – 274Combined sources5
Beta strandi280 – 286Combined sources7
Helixi288 – 294Combined sources7
Helixi296 – 300Combined sources5
Turni301 – 303Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OM0X-ray1.80A31-304[»]
1TA3X-ray1.70A31-304[»]
1TE1X-ray2.50A31-304[»]
ProteinModelPortaliQ8L5C6.
SMRiQ8L5C6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8L5C6.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni178 – 184Interaction with fungal GH11 xylanase7
Regioni262 – 275Interaction with fungal GH10 xylanaseAdd BLAST14

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

OMAiYDDADCE.
OrthoDBiEOG09360IF6.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001223. Glyco_hydro18_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L5C6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPLAARRPA CLLALLSVAA ALFLTPTALA AGGKTGQVTV FWGRNKAEGS
60 70 80 90 100
LREACDSGMY TMVTMSFLDV FGANGKYHLD LSGHDLSSVG ADIKHCQSKG
110 120 130 140 150
VPVSLSIGGY GTGYSLPSNR SALDLFDHLW NSYFGGSKPS VPRPFGDAWL
160 170 180 190 200
DGVDLFLEHG TPADRYDVLA LELAKHNIRG GPGKPLHLTA TVRCGYPPAA
210 220 230 240 250
HVGRALATGI FERVHVRTYE SDKWCNQNLG WEGSWDKWTA AYPATRFYVG
260 270 280 290 300
LTADDKSHQW VHPKNVYYGV APVAQKKDNY GGIMLWDRYF DKQTNYSSLI

KYYA
Length:304
Mass (Da):33,275
Last modified:August 16, 2005 - v2
Checksum:iFA0FEF12FE853E30
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67F → L in CAD19479 (PubMed:12023019).Curated1
Sequence conflicti214V → A in CAD19479 (PubMed:12023019).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ422119 mRNA. Translation: CAD19479.1.
UniGeneiTa.38246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ422119 mRNA. Translation: CAD19479.1.
UniGeneiTa.38246.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OM0X-ray1.80A31-304[»]
1TA3X-ray1.70A31-304[»]
1TE1X-ray2.50A31-304[»]
ProteinModelPortaliQ8L5C6.
SMRiQ8L5C6.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei9596. Tri a XI.
CAZyiGH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OMAiYDDADCE.
OrthoDBiEOG09360IF6.

Miscellaneous databases

EvolutionaryTraceiQ8L5C6.

Gene expression databases

ExpressionAtlasiQ8L5C6. baseline and differential.
GenevisibleiQ8L5C6. TA.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001223. Glyco_hydro18_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiXIP1_WHEAT
AccessioniPrimary (citable) accession number: Q8L5C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: November 2, 2016
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.