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Protein

5-formyltetrahydrofolate cyclo-ligase, mitochondrial

Gene

5FCL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to tetrahydrofolate metabolism and photorespiration through the regulation of serine hydroxymethyltransferase. Prefers the pentalutamyl to the monoglutamyl form of 5-formyltetrahydrofolate.2 Publications

Catalytic activityi

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.1 Publication

Kineticsi

  1. KM=5.8 µM for 5-formyltetrahydrofolate1 Publication
  2. KM=35.2 µM for ATP1 Publication

Vmax=11.5 µmol/min/mg enzyme toward 5-formyltetrahydrofolate1 Publication

pH dependencei

Optimum pH is 8.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei254 – 2541ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi60 – 645ATPBy similarity
Nucleotide bindingi206 – 2138ATPBy similarity

GO - Molecular functioni

  1. 5-formyltetrahydrofolate cyclo-ligase activity Source: TAIR
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. tetrahydrofolate metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G13050-MONOMER.
BRENDAi6.3.3.2. 302.
SABIO-RKQ8L539.

Names & Taxonomyi

Protein namesi
Recommended name:
5-formyltetrahydrofolate cyclo-ligase, mitochondrial (EC:6.3.3.2)
Short name:
5-FCL
Gene namesi
Name:5FCL
Ordered Locus Names:At5g13050
ORF Names:T19L5.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G13050.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Reduced growth rate and delayed flowering.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848MitochondrionSequence AnalysisAdd
BLAST
Chaini49 – 2772295-formyltetrahydrofolate cyclo-ligase, mitochondrialPRO_0000428719Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriQ8L539.

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8L539.
SMRiQ8L539. Positions 79-266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni207 – 2115Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000007299.
InParanoidiQ8L539.
KOiK01934.
OMAiVLEAPWF.
PhylomeDBiQ8L539.

Family and domain databases

Gene3Di3.40.50.10420. 1 hit.
InterProiIPR002698. FTHF_cligase.
IPR024185. FTHF_cligase-like.
[Graphical view]
PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02727. MTHFS_bact. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8L539-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIGARVFCIT TTALRRSPIF FFPKIPTRPV FRLSPATRPI VAMSTTSKNQ
60 70 80 90 100
EELDSIFKQK RVVRSTVRKS LKAMDPSLRT QQDEAIQKTV LEAPWFKSCK
110 120 130 140 150
GLCAYISCKS LNEVDTSKIL SEILQHPDSN TQKKLYVPWV EDKNSNMRML
160 170 180 190 200
HISHMEDLVA NSMNILEPAP VDAQGNDRED VLQADEPIDL FILPGLAFDR
210 220 230 240 250
CGRRLGRGGG YYDTFLKRYQ DRAKEKGWRY PLMVALSYSP QILEDGSIPV
260 270
TPNDVLIDAL VTPSGVVPIT PRATESM
Length:277
Mass (Da):31,233
Last modified:October 1, 2002 - v1
Checksum:i221250EBE1D074FE
GO
Isoform 2 (identifier: Q8L539-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     209-214: GGYYDT → ATTTLS
     215-277: Missing.

Note: No experimental confirmation available.

Show »
Length:214
Mass (Da):24,137
Checksum:iDCC4C243A7359CBC
GO

Sequence cautioni

The sequence CAC05433.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei209 – 2146GGYYDT → ATTTLS in isoform 2. 1 PublicationVSP_054249
Alternative sequencei215 – 27763Missing in isoform 2. 1 PublicationVSP_054250Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF516365 mRNA. Translation: AAM90960.1.
AL391711 Genomic DNA. Translation: CAC05433.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED91844.1.
CP002688 Genomic DNA. Translation: AED91845.1.
BX830290 mRNA. No translation available.
BT024502 mRNA. Translation: ABD19683.1.
AY084396 mRNA. Translation: AAM60972.1.
BT003924 mRNA. Translation: AAO41971.1.
RefSeqiNP_001031873.1. NM_001036796.2. [Q8L539-2]
NP_568284.1. NM_121308.2. [Q8L539-1]
UniGeneiAt.20443.
At.71082.

Genome annotation databases

EnsemblPlantsiAT5G13050.1; AT5G13050.1; AT5G13050. [Q8L539-1]
AT5G13050.2; AT5G13050.2; AT5G13050. [Q8L539-2]
GeneIDi831144.
KEGGiath:AT5G13050.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF516365 mRNA. Translation: AAM90960.1.
AL391711 Genomic DNA. Translation: CAC05433.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED91844.1.
CP002688 Genomic DNA. Translation: AED91845.1.
BX830290 mRNA. No translation available.
BT024502 mRNA. Translation: ABD19683.1.
AY084396 mRNA. Translation: AAM60972.1.
BT003924 mRNA. Translation: AAO41971.1.
RefSeqiNP_001031873.1. NM_001036796.2. [Q8L539-2]
NP_568284.1. NM_121308.2. [Q8L539-1]
UniGeneiAt.20443.
At.71082.

3D structure databases

ProteinModelPortaliQ8L539.
SMRiQ8L539. Positions 79-266.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G13050.1; AT5G13050.1; AT5G13050. [Q8L539-1]
AT5G13050.2; AT5G13050.2; AT5G13050. [Q8L539-2]
GeneIDi831144.
KEGGiath:AT5G13050.

Organism-specific databases

TAIRiAT5G13050.

Phylogenomic databases

HOGENOMiHOG000007299.
InParanoidiQ8L539.
KOiK01934.
OMAiVLEAPWF.
PhylomeDBiQ8L539.

Enzyme and pathway databases

BioCyciARA:AT5G13050-MONOMER.
BRENDAi6.3.3.2. 302.
SABIO-RKQ8L539.

Miscellaneous databases

PROiQ8L539.

Gene expression databases

GenevestigatoriQ8L539.

Family and domain databases

Gene3Di3.40.50.10420. 1 hit.
InterProiIPR002698. FTHF_cligase.
IPR024185. FTHF_cligase-like.
[Graphical view]
PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02727. MTHFS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of mitochondrial 5-formyltetrahydrofolate cycloligase from higher plants."
    Roje S., Janave M.T., Ziemak M.J., Hanson A.D.
    J. Biol. Chem. 277:42748-42754(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Ecker J.R.
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-277 (ISOFORM 2).
    Strain: cv. Columbia.
  7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-277 (ISOFORM 1).
    Strain: cv. Columbia.
  8. "5-Formyltetrahydrofolate is an inhibitory but well tolerated metabolite in Arabidopsis leaves."
    Goyer A., Collakova E., Diaz de la Garza R., Quinlivan E.P., Williamson J., Gregory J.F., Shachar-Hill Y., Hanson A.D.
    J. Biol. Chem. 280:26137-26142(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry namei5FCL_ARATH
AccessioniPrimary (citable) accession number: Q8L539
Secondary accession number(s): F4K2F3, Q84WD9, Q9FYA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: October 1, 2002
Last modified: January 7, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.