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Q8L539

- 5FCL_ARATH

UniProt

Q8L539 - 5FCL_ARATH

Protein

5-formyltetrahydrofolate cyclo-ligase, mitochondrial

Gene

5FCL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Contributes to tetrahydrofolate metabolism and photorespiration through the regulation of serine hydroxymethyltransferase. Prefers the pentalutamyl to the monoglutamyl form of 5-formyltetrahydrofolate.2 Publications

    Catalytic activityi

    ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.1 Publication

    Kineticsi

    1. KM=5.8 µM for 5-formyltetrahydrofolate1 Publication
    2. KM=35.2 µM for ATP1 Publication

    Vmax=11.5 µmol/min/mg enzyme toward 5-formyltetrahydrofolate1 Publication

    pH dependencei

    Optimum pH is 8.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei254 – 2541ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi60 – 645ATPBy similarity
    Nucleotide bindingi206 – 2138ATPBy similarity

    GO - Molecular functioni

    1. 5-formyltetrahydrofolate cyclo-ligase activity Source: TAIR
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. folic acid-containing compound biosynthetic process Source: InterPro
    2. tetrahydrofolate metabolic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G13050-MONOMER.
    BRENDAi6.3.3.2. 302.
    SABIO-RKQ8L539.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-formyltetrahydrofolate cyclo-ligase, mitochondrial (EC:6.3.3.2)
    Short name:
    5-FCL
    Gene namesi
    Name:5FCL
    Ordered Locus Names:At5g13050
    ORF Names:T19L5.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G13050.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Reduced growth rate and delayed flowering.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4848MitochondrionSequence AnalysisAdd
    BLAST
    Chaini49 – 2772295-formyltetrahydrofolate cyclo-ligase, mitochondrialPRO_0000428719Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriQ8L539.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L539.
    SMRiQ8L539. Positions 79-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni207 – 2115Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000007299.
    InParanoidiQ8L539.
    KOiK01934.
    OMAiVLEAPWF.
    PhylomeDBiQ8L539.

    Family and domain databases

    Gene3Di3.40.50.10420. 1 hit.
    InterProiIPR002698. FTHF_cligase.
    IPR024185. FTHF_cligase-like.
    [Graphical view]
    PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
    PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02727. MTHFS_bact. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8L539-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MIGARVFCIT TTALRRSPIF FFPKIPTRPV FRLSPATRPI VAMSTTSKNQ    50
    EELDSIFKQK RVVRSTVRKS LKAMDPSLRT QQDEAIQKTV LEAPWFKSCK 100
    GLCAYISCKS LNEVDTSKIL SEILQHPDSN TQKKLYVPWV EDKNSNMRML 150
    HISHMEDLVA NSMNILEPAP VDAQGNDRED VLQADEPIDL FILPGLAFDR 200
    CGRRLGRGGG YYDTFLKRYQ DRAKEKGWRY PLMVALSYSP QILEDGSIPV 250
    TPNDVLIDAL VTPSGVVPIT PRATESM 277
    Length:277
    Mass (Da):31,233
    Last modified:October 1, 2002 - v1
    Checksum:i221250EBE1D074FE
    GO
    Isoform 2 (identifier: Q8L539-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         209-214: GGYYDT → ATTTLS
         215-277: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:214
    Mass (Da):24,137
    Checksum:iDCC4C243A7359CBC
    GO

    Sequence cautioni

    The sequence CAC05433.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei209 – 2146GGYYDT → ATTTLS in isoform 2. 1 PublicationVSP_054249
    Alternative sequencei215 – 27763Missing in isoform 2. 1 PublicationVSP_054250Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF516365 mRNA. Translation: AAM90960.1.
    AL391711 Genomic DNA. Translation: CAC05433.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED91844.1.
    CP002688 Genomic DNA. Translation: AED91845.1.
    BX830290 mRNA. No translation available.
    BT024502 mRNA. Translation: ABD19683.1.
    AY084396 mRNA. Translation: AAM60972.1.
    BT003924 mRNA. Translation: AAO41971.1.
    RefSeqiNP_001031873.1. NM_001036796.2. [Q8L539-2]
    NP_568284.1. NM_121308.2. [Q8L539-1]
    UniGeneiAt.20443.
    At.71082.

    Genome annotation databases

    EnsemblPlantsiAT5G13050.1; AT5G13050.1; AT5G13050. [Q8L539-1]
    AT5G13050.2; AT5G13050.2; AT5G13050. [Q8L539-2]
    GeneIDi831144.
    KEGGiath:AT5G13050.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF516365 mRNA. Translation: AAM90960.1 .
    AL391711 Genomic DNA. Translation: CAC05433.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED91844.1 .
    CP002688 Genomic DNA. Translation: AED91845.1 .
    BX830290 mRNA. No translation available.
    BT024502 mRNA. Translation: ABD19683.1 .
    AY084396 mRNA. Translation: AAM60972.1 .
    BT003924 mRNA. Translation: AAO41971.1 .
    RefSeqi NP_001031873.1. NM_001036796.2. [Q8L539-2 ]
    NP_568284.1. NM_121308.2. [Q8L539-1 ]
    UniGenei At.20443.
    At.71082.

    3D structure databases

    ProteinModelPortali Q8L539.
    SMRi Q8L539. Positions 79-266.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G13050.1 ; AT5G13050.1 ; AT5G13050 . [Q8L539-1 ]
    AT5G13050.2 ; AT5G13050.2 ; AT5G13050 . [Q8L539-2 ]
    GeneIDi 831144.
    KEGGi ath:AT5G13050.

    Organism-specific databases

    TAIRi AT5G13050.

    Phylogenomic databases

    HOGENOMi HOG000007299.
    InParanoidi Q8L539.
    KOi K01934.
    OMAi VLEAPWF.
    PhylomeDBi Q8L539.

    Enzyme and pathway databases

    BioCyci ARA:AT5G13050-MONOMER.
    BRENDAi 6.3.3.2. 302.
    SABIO-RK Q8L539.

    Miscellaneous databases

    PROi Q8L539.

    Gene expression databases

    Genevestigatori Q8L539.

    Family and domain databases

    Gene3Di 3.40.50.10420. 1 hit.
    InterProi IPR002698. FTHF_cligase.
    IPR024185. FTHF_cligase-like.
    [Graphical view ]
    PANTHERi PTHR23407:SF1. PTHR23407:SF1. 1 hit.
    Pfami PF01812. 5-FTHF_cyc-lig. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02727. MTHFS_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of mitochondrial 5-formyltetrahydrofolate cycloligase from higher plants."
      Roje S., Janave M.T., Ziemak M.J., Hanson A.D.
      J. Biol. Chem. 277:42748-42754(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Shinn P., Chen H., Kim C.J., Ecker J.R.
      Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
      Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
      Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-277 (ISOFORM 2).
      Strain: cv. Columbia.
    7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-277 (ISOFORM 1).
      Strain: cv. Columbia.
    8. "5-Formyltetrahydrofolate is an inhibitory but well tolerated metabolite in Arabidopsis leaves."
      Goyer A., Collakova E., Diaz de la Garza R., Quinlivan E.P., Williamson J., Gregory J.F., Shachar-Hill Y., Hanson A.D.
      J. Biol. Chem. 280:26137-26142(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry namei5FCL_ARATH
    AccessioniPrimary (citable) accession number: Q8L539
    Secondary accession number(s): F4K2F3, Q84WD9, Q9FYA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3