Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable choline kinase 2

Gene

At1g74320

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in phospholipid biosynthesis. Catalyzes the first step in phosphatidylcholine biosynthesis (By similarity).By similarity

Catalytic activityi

ATP + choline = ADP + phosphocholine.

Pathway: phosphatidylcholine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphocholine from choline.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable choline kinase 2 (At1g74320), Probable choline kinase 3 (At4g09760), Probable choline kinase 1 (CK1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphocholine from choline, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei73 – 731ATPBy similarity
Binding sitei210 – 2101ATPBy similarity
Binding sitei227 – 2271ATPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G74320-MONOMER.
ReactomeiREACT_279011. Synthesis of PE.
REACT_348151. Synthesis of PC.
UniPathwayiUPA00753; UER00737.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable choline kinase 2 (EC:2.7.1.32)
Gene namesi
Ordered Locus Names:At1g74320
ORF Names:F1O17.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G74320.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350Probable choline kinase 2PRO_0000423347Add
BLAST

Proteomic databases

PRIDEiQ8L518.

Expressioni

Inductioni

By wounding, and salt and osmotic stresses.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G74320.1.

Structurei

3D structure databases

ProteinModelPortaliQ8L518.
SMRiQ8L518. Positions 46-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiCOG0510.
HOGENOMiHOG000041274.
InParanoidiQ8L518.
KOiK14156.
OMAiEAKEFCL.
PhylomeDBiQ8L518.

Family and domain databases

InterProiIPR026712. Cho/Etha_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERiPTHR22603:SF32. PTHR22603:SF32. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8L518-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMGGTEKNV ENKQYRLPRE VKEALQAIAS EWEDVIDSKA LQVIPLKGAM
60 70 80 90 100
TNEVFQIKWP TREKGPSRKV LVRIYGEGVE IFFDREDEIR TFEFMSKHGH
110 120 130 140 150
GPLLLGRFGN GRIEEFLHAR TLSACDLRDP EISGRIATRM KEFHGLEMPG
160 170 180 190 200
AKKALLWDRL RNWLTACKRL ASPEEAKSFR LDVMEMEINM LEKSLFDNDE
210 220 230 240 250
NIGFCHNDLQ YGNIMMDEET KAITIIDYEY SCYNPVAYDI ANHFCEMAAD
260 270 280 290 300
YHTETPHIMD YSKYPGVEER QRFLKTYMSY SDEKPSDTMV KKLLEDVEKY
310 320 330 340 350
TLASHLIWGL WGIISEHVNE IDFDYMEYAR QRFEQYWLTK PRLLAASEHK
Length:350
Mass (Da):41,017
Last modified:October 1, 2002 - v1
Checksum:i2C38393ADA5D2465
GO

Sequence cautioni

The sequence AAG52400.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC020579 Genomic DNA. Translation: AAG52400.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE35577.1.
AY094430 mRNA. Translation: AAM19803.1.
AY140093 mRNA. Translation: AAM98234.1.
BT008737 mRNA. Translation: AAP42750.1.
PIRiG96771.
RefSeqiNP_177572.2. NM_106092.3.
UniGeneiAt.43684.

Genome annotation databases

EnsemblPlantsiAT1G74320.1; AT1G74320.1; AT1G74320.
GeneIDi843772.
KEGGiath:AT1G74320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC020579 Genomic DNA. Translation: AAG52400.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE35577.1.
AY094430 mRNA. Translation: AAM19803.1.
AY140093 mRNA. Translation: AAM98234.1.
BT008737 mRNA. Translation: AAP42750.1.
PIRiG96771.
RefSeqiNP_177572.2. NM_106092.3.
UniGeneiAt.43684.

3D structure databases

ProteinModelPortaliQ8L518.
SMRiQ8L518. Positions 46-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G74320.1.

Proteomic databases

PRIDEiQ8L518.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G74320.1; AT1G74320.1; AT1G74320.
GeneIDi843772.
KEGGiath:AT1G74320.

Organism-specific databases

TAIRiAT1G74320.

Phylogenomic databases

eggNOGiCOG0510.
HOGENOMiHOG000041274.
InParanoidiQ8L518.
KOiK14156.
OMAiEAKEFCL.
PhylomeDBiQ8L518.

Enzyme and pathway databases

UniPathwayiUPA00753; UER00737.
BioCyciARA:AT1G74320-MONOMER.
ReactomeiREACT_279011. Synthesis of PE.
REACT_348151. Synthesis of PC.

Miscellaneous databases

PROiQ8L518.

Family and domain databases

InterProiIPR026712. Cho/Etha_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERiPTHR22603:SF32. PTHR22603:SF32. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Regulation of phosphatidylcholine biosynthesis under salt stress involves choline kinases in Arabidopsis thaliana."
    Tasseva G., Richard L., Zachowski A.
    FEBS Lett. 566:115-120(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiCK2_ARATH
AccessioniPrimary (citable) accession number: Q8L518
Secondary accession number(s): Q9C916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: October 1, 2002
Last modified: June 24, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.