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Q8L500

- APRR9_ARATH

UniProt

Q8L500 - APRR9_ARATH

Protein

Two-component response regulator-like APRR9

Gene

APRR9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (27 Sep 2004)
      Previous versions | rss
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    Functioni

    Transcriptional repressor of CCA1 and LHY, and positive regulator of LWD1 and LWD2 expression. Controls photoperiodic flowering response and temperature compensation. Involved in the positive and negative feedback loops of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. Regulated at the transcriptional level by a corepressor complex consisting of ELF4, ELF3, and LUX. APRR9, APRR7, and APRR5 coordinately act on the upstream region of the target genes to repress their expression from noon until midnight. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock.5 Publications

    GO - Molecular functioni

    1. DNA binding Source: TAIR
    2. phosphorelay response regulator activity Source: InterPro
    3. protein binding Source: TAIR

    GO - Biological processi

    1. circadian rhythm Source: TAIR
    2. negative regulation of transcription, DNA-templated Source: TAIR
    3. red or far-red light signaling pathway Source: TAIR
    4. regulation of transcription, DNA-templated Source: TAIR
    5. response to temperature stimulus Source: TAIR
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Biological rhythms, Transcription, Transcription regulation, Two-component regulatory system

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Two-component response regulator-like APRR9
    Alternative name(s):
    Pseudo-response regulator 9
    Gene namesi
    Name:APRR9
    Ordered Locus Names:At2g46790
    ORF Names:F19D11.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G46790.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 468468Two-component response regulator-like APRR9PRO_0000081441Add
    BLAST

    Post-translational modificationi

    Phosphorylated. Phosphorylation varies throughout the diurnal cycle.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ8L500.

    Expressioni

    Inductioni

    Up-regulated by heat-shock.2 Publications

    Gene expression databases

    GenevestigatoriQ8L500.

    Interactioni

    Protein-protein interaction databases

    BioGridi4627. 6 interactions.
    IntActiQ8L500. 1 interaction.
    MINTiMINT-7908741.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L500.
    SMRiQ8L500. Positions 39-200.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 156119Response regulatoryPROSITE-ProRule annotationAdd
    BLAST
    Domaini417 – 45943CCTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ARR-like family.Curated
    Contains 1 CCT domain.PROSITE-ProRule annotation
    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0745.
    HOGENOMiHOG000034018.
    InParanoidiQ8L500.
    KOiK12128.
    OMAiHEACKNI.
    PhylomeDBiQ8L500.

    Family and domain databases

    InterProiIPR010402. CCT_domain.
    IPR011006. CheY-like_superfamily.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view]
    PfamiPF06203. CCT. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view]
    SMARTiSM00448. REC. 1 hit.
    [Graphical view]
    SUPFAMiSSF52172. SSF52172. 1 hit.
    PROSITEiPS51017. CCT. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q8L500-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGEIVVLSSD DGMETIKNRV KSSEVVQWEK YLPKTVLRVL LVESDYSTRQ    50
    IITALLRKCC YKVVAVSDGL AAWEVLKEKS HNIDLILTEL DLPSISGFAL 100
    LALVMEHEAC KNIPVIMMSS QDSIKMVLKC MLRGAADYLI KPMRKNELKN 150
    LWQHVWRRLT LRDDPTAHAQ SLPASQHNLE DTDETCEDSR YHSDQGSGAQ 200
    AINYNGHNKL MENGKSVDER DEFKETFDVT MDLIGGIDKR PDSIYKDKSR 250
    DECVGPELGL SLKRSCSVSF ENQDESKHQK LSLSDASAFS RFEESKSAEK 300
    AVVALEESTS GEPKTPTESH EKLRKVTSDQ GSATTSSNQE NIGSSSVSFR 350
    NQVLQSTVTN QKQDSPIPVE SNREKAASKE VEAGSQSTNE GIAGQSSSTE 400
    KPKEEESAKQ RWSRSQREAA LMKFRLKRKD RCFDKKVRYQ SRKKLAEQRP 450
    RVKGQFVRTV NSDASTKS 468
    Length:468
    Mass (Da):52,565
    Last modified:September 27, 2004 - v2
    Checksum:i19D6C9571CA77A40
    GO

    Sequence cautioni

    The sequence AAM20527.1 differs from that shown. Reason: Frameshift at position 117.
    The sequence AAM91256.1 differs from that shown. Reason: Frameshift at position 117.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB046953 mRNA. Translation: BAB13741.1.
    AF272040 mRNA. Translation: AAF86253.1.
    AC005310 Genomic DNA. Translation: AAC33497.2.
    CP002685 Genomic DNA. Translation: AEC10754.1.
    AY099676 mRNA. Translation: AAM20527.1. Frameshift.
    AY128856 mRNA. Translation: AAM91256.1. Frameshift.
    PIRiT02680.
    RefSeqiNP_566085.1. NM_130245.4. [Q8L500-1]
    UniGeneiAt.1675.
    At.65052.

    Genome annotation databases

    EnsemblPlantsiAT2G46790.1; AT2G46790.1; AT2G46790. [Q8L500-1]
    GeneIDi819292.
    KEGGiath:AT2G46790.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB046953 mRNA. Translation: BAB13741.1 .
    AF272040 mRNA. Translation: AAF86253.1 .
    AC005310 Genomic DNA. Translation: AAC33497.2 .
    CP002685 Genomic DNA. Translation: AEC10754.1 .
    AY099676 mRNA. Translation: AAM20527.1 . Frameshift.
    AY128856 mRNA. Translation: AAM91256.1 . Frameshift.
    PIRi T02680.
    RefSeqi NP_566085.1. NM_130245.4. [Q8L500-1 ]
    UniGenei At.1675.
    At.65052.

    3D structure databases

    ProteinModelPortali Q8L500.
    SMRi Q8L500. Positions 39-200.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4627. 6 interactions.
    IntActi Q8L500. 1 interaction.
    MINTi MINT-7908741.

    Proteomic databases

    PRIDEi Q8L500.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G46790.1 ; AT2G46790.1 ; AT2G46790 . [Q8L500-1 ]
    GeneIDi 819292.
    KEGGi ath:AT2G46790.

    Organism-specific databases

    GeneFarmi 4360. 437.
    TAIRi AT2G46790.

    Phylogenomic databases

    eggNOGi COG0745.
    HOGENOMi HOG000034018.
    InParanoidi Q8L500.
    KOi K12128.
    OMAi HEACKNI.
    PhylomeDBi Q8L500.

    Gene expression databases

    Genevestigatori Q8L500.

    Family and domain databases

    InterProi IPR010402. CCT_domain.
    IPR011006. CheY-like_superfamily.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view ]
    Pfami PF06203. CCT. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view ]
    SMARTi SM00448. REC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52172. SSF52172. 1 hit.
    PROSITEi PS51017. CCT. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana: insight into the plant circadian clock."
      Matsushika A., Makino S., Kojima M., Mizuno T.
      Plant Cell Physiol. 41:1002-1012(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    2. "Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog."
      Strayer C., Oyama T., Schultz T.F., Raman R., Somers D.E., Mas P., Panda S., Kreps J.A., Kay S.A.
      Science 289:768-771(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana."
      Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T., Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T.
      Plant Cell Physiol. 41:791-803(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    7. "Post-translational regulation of the Arabidopsis circadian clock through selective proteolysis and phosphorylation of pseudo-response regulator proteins."
      Fujiwara S., Wang L., Han L., Suh S.-S., Salome P.A., McClung C.R., Somers D.E.
      J. Biol. Chem. 283:23073-23083(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION.
    8. Cited for: REGULATION.
    9. "PSEUDO-RESPONSE REGULATORS 9, 7, and 5 are transcriptional repressors in the Arabidopsis circadian clock."
      Nakamichi N., Kiba T., Henriques R., Mizuno T., Chua N.H., Sakakibara H.
      Plant Cell 22:594-605(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "The role of the Arabidopsis morning loop components CCA1, LHY, PRR7, and PRR9 in temperature compensation."
      Salome P.A., Weigel D., McClung C.R.
      Plant Cell 22:3650-3661(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "LIGHT-REGULATED WD1 and PSEUDO-RESPONSE REGULATOR9 form a positive feedback regulatory loop in the Arabidopsis circadian clock."
      Wang Y., Wu J.F., Nakamichi N., Sakakibara H., Nam H.G., Wu S.H.
      Plant Cell 23:486-498(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    12. "ELF3 recruitment to the PRR9 promoter requires other Evening Complex members in the Arabidopsis circadian clock."
      Chow B.Y., Helfer A., Nusinow D.A., Kay S.A.
      Plant Signal. Behav. 7:170-173(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: REGULATION.
    13. "EARLY FLOWERING4 recruitment of EARLY FLOWERING3 in the nucleus sustains the Arabidopsis circadian clock."
      Herrero E., Kolmos E., Bujdoso N., Yuan Y., Wang M., Berns M.C., Uhlworm H., Coupland G., Saini R., Jaskolski M., Webb A., Goncalves J., Davis S.J.
      Plant Cell 24:428-443(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: REGULATION.
    14. "SKIP is a component of the spliceosome linking alternative splicing and the circadian clock in Arabidopsis."
      Wang X., Wu F., Xie Q., Wang H., Wang Y., Yue Y., Gahura O., Ma S., Liu L., Cao Y., Jiao Y., Puta F., McClung C.R., Xu X., Ma L.
      Plant Cell 24:3278-3295(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: REGULATION.
    15. Cited for: FUNCTION.
    16. "Heat shock-induced fluctuations in clock and light signaling enhance phytochrome B-mediated Arabidopsis deetiolation."
      Karayekov E., Sellaro R., Legris M., Yanovsky M.J., Casal J.J.
      Plant Cell 25:2892-2906(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY HEAT-SHOCK.

    Entry informationi

    Entry nameiAPRR9_ARATH
    AccessioniPrimary (citable) accession number: Q8L500
    Secondary accession number(s): O81035, Q9LKL1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2004
    Last sequence update: September 27, 2004
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Regulated at the level of mRNA maturation and alternative splicing by SKIP (PubMed:22942380) and PRMT5 (PubMed:20962777). The expression of APRR9, APRR7, and APRR5 requires the presence of LWD1 and/or LWD2, indicating the existence of a positive feedback loop within the circadian clock (PubMed:21357491).3 Publications

    Caution

    Lacks the phospho-accepting Asp (here Glu-89), present in the receiver domain, which is one of the conserved features of two-component response regulators (ARRs) family.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3