Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8L500 (APRR9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Two-component response regulator-like APRR9
Alternative name(s):
Pseudo-response regulator 9
Gene names
Name:APRR9
Ordered Locus Names:At2g46790
ORF Names:F19D11.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional repressor of CCA1 and LHY, and positive regulator of LWD1 and LWD2 expression. Controls photoperiodic flowering response and temperature compensation. Involved in the positive and negative feedback loops of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. Regulated at the transcriptional level by a corepressor complex consisting of ELF4, ELF3, and LUX. APRR9, APRR7, and APRR5 coordinately act on the upstream region of the target genes to repress their expression from noon until midnight. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. Ref.1 Ref.9 Ref.10 Ref.11 Ref.15

Subcellular location

Nucleus Probable.

Induction

Up-regulated by heat-shock. Ref.8 Ref.11 Ref.12 Ref.13 Ref.14 Ref.16

Post-translational modification

Phosphorylated. Phosphorylation varies throughout the diurnal cycle. Ref.7

Miscellaneous

Regulated at the level of mRNA maturation and alternative splicing by SKIP (Ref.14) and PRMT5 (Ref.8). The expression of APRR9, APRR7, and APRR5 requires the presence of LWD1 and/or LWD2, indicating the existence of a positive feedback loop within the circadian clock (Ref.11).

Sequence similarities

Belongs to the ARR-like family.

Contains 1 CCT domain.

Contains 1 response regulatory domain.

Caution

Lacks the phospho-accepting Asp (here Glu-89), present in the receiver domain, which is one of the conserved features of two-component response regulators (ARRs) family.

Sequence caution

The sequence AAM20527.1 differs from that shown. Reason: Frameshift at position 117.

The sequence AAM91256.1 differs from that shown. Reason: Frameshift at position 117.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8L500-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468Two-component response regulator-like APRR9
PRO_0000081441

Regions

Domain38 – 156119Response regulatory
Domain417 – 45943CCT

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 27, 2004. Version 2.
Checksum: 19D6C9571CA77A40

FASTA46852,565
        10         20         30         40         50         60 
MGEIVVLSSD DGMETIKNRV KSSEVVQWEK YLPKTVLRVL LVESDYSTRQ IITALLRKCC 

        70         80         90        100        110        120 
YKVVAVSDGL AAWEVLKEKS HNIDLILTEL DLPSISGFAL LALVMEHEAC KNIPVIMMSS 

       130        140        150        160        170        180 
QDSIKMVLKC MLRGAADYLI KPMRKNELKN LWQHVWRRLT LRDDPTAHAQ SLPASQHNLE 

       190        200        210        220        230        240 
DTDETCEDSR YHSDQGSGAQ AINYNGHNKL MENGKSVDER DEFKETFDVT MDLIGGIDKR 

       250        260        270        280        290        300 
PDSIYKDKSR DECVGPELGL SLKRSCSVSF ENQDESKHQK LSLSDASAFS RFEESKSAEK 

       310        320        330        340        350        360 
AVVALEESTS GEPKTPTESH EKLRKVTSDQ GSATTSSNQE NIGSSSVSFR NQVLQSTVTN 

       370        380        390        400        410        420 
QKQDSPIPVE SNREKAASKE VEAGSQSTNE GIAGQSSSTE KPKEEESAKQ RWSRSQREAA 

       430        440        450        460 
LMKFRLKRKD RCFDKKVRYQ SRKKLAEQRP RVKGQFVRTV NSDASTKS 

« Hide

References

« Hide 'large scale' references
[1]"Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana: insight into the plant circadian clock."
Matsushika A., Makino S., Kojima M., Mizuno T.
Plant Cell Physiol. 41:1002-1012(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog."
Strayer C., Oyama T., Schultz T.F., Raman R., Somers D.E., Mas P., Panda S., Kreps J.A., Kay S.A.
Science 289:768-771(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana."
Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T., Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T.
Plant Cell Physiol. 41:791-803(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[7]"Post-translational regulation of the Arabidopsis circadian clock through selective proteolysis and phosphorylation of pseudo-response regulator proteins."
Fujiwara S., Wang L., Han L., Suh S.-S., Salome P.A., McClung C.R., Somers D.E.
J. Biol. Chem. 283:23073-23083(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION.
[8]"A methyl transferase links the circadian clock to the regulation of alternative splicing."
Sanchez S.E., Petrillo E., Beckwith E.J., Zhang X., Rugnone M.L., Hernando C.E., Cuevas J.C., Godoy Herz M.A., Depetris-Chauvin A., Simpson C.G., Brown J.W., Cerdan P.D., Borevitz J.O., Mas P., Ceriani M.F., Kornblihtt A.R., Yanovsky M.J.
Nature 468:112-116(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: REGULATION.
[9]"PSEUDO-RESPONSE REGULATORS 9, 7, and 5 are transcriptional repressors in the Arabidopsis circadian clock."
Nakamichi N., Kiba T., Henriques R., Mizuno T., Chua N.H., Sakakibara H.
Plant Cell 22:594-605(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"The role of the Arabidopsis morning loop components CCA1, LHY, PRR7, and PRR9 in temperature compensation."
Salome P.A., Weigel D., McClung C.R.
Plant Cell 22:3650-3661(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"LIGHT-REGULATED WD1 and PSEUDO-RESPONSE REGULATOR9 form a positive feedback regulatory loop in the Arabidopsis circadian clock."
Wang Y., Wu J.F., Nakamichi N., Sakakibara H., Nam H.G., Wu S.H.
Plant Cell 23:486-498(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[12]"ELF3 recruitment to the PRR9 promoter requires other Evening Complex members in the Arabidopsis circadian clock."
Chow B.Y., Helfer A., Nusinow D.A., Kay S.A.
Plant Signal. Behav. 7:170-173(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: REGULATION.
[13]"EARLY FLOWERING4 recruitment of EARLY FLOWERING3 in the nucleus sustains the Arabidopsis circadian clock."
Herrero E., Kolmos E., Bujdoso N., Yuan Y., Wang M., Berns M.C., Uhlworm H., Coupland G., Saini R., Jaskolski M., Webb A., Goncalves J., Davis S.J.
Plant Cell 24:428-443(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: REGULATION.
[14]"SKIP is a component of the spliceosome linking alternative splicing and the circadian clock in Arabidopsis."
Wang X., Wu F., Xie Q., Wang H., Wang Y., Yue Y., Gahura O., Ma S., Liu L., Cao Y., Jiao Y., Puta F., McClung C.R., Xu X., Ma L.
Plant Cell 24:3278-3295(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: REGULATION.
[15]"Transcriptional repressor PRR5 directly regulates clock-output pathways."
Nakamichi N., Kiba T., Kamioka M., Suzuki T., Yamashino T., Higashiyama T., Sakakibara H., Mizuno T.
Proc. Natl. Acad. Sci. U.S.A. 109:17123-17128(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[16]"Heat shock-induced fluctuations in clock and light signaling enhance phytochrome B-mediated Arabidopsis deetiolation."
Karayekov E., Sellaro R., Legris M., Yanovsky M.J., Casal J.J.
Plant Cell 25:2892-2906(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY HEAT-SHOCK.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB046953 mRNA. Translation: BAB13741.1.
AF272040 mRNA. Translation: AAF86253.1.
AC005310 Genomic DNA. Translation: AAC33497.2.
CP002685 Genomic DNA. Translation: AEC10754.1.
AY099676 mRNA. Translation: AAM20527.1. Frameshift.
AY128856 mRNA. Translation: AAM91256.1. Frameshift.
PIRT02680.
RefSeqNP_566085.1. NM_130245.4.
UniGeneAt.1675.
At.65052.

3D structure databases

ProteinModelPortalQ8L500.
SMRQ8L500. Positions 39-200.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4627. 5 interactions.
IntActQ8L500. 1 interaction.
MINTMINT-7908741.

Proteomic databases

PRIDEQ8L500.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G46790.1; AT2G46790.1; AT2G46790. [Q8L500-1]
GeneID819292.
KEGGath:AT2G46790.

Organism-specific databases

GeneFarm4360. 437.
TAIRAT2G46790.

Phylogenomic databases

eggNOGCOG0745.
HOGENOMHOG000034018.
InParanoidQ8L500.
KOK12128.
OMAHEACKNI.
PhylomeDBQ8L500.
ProtClustDBCLSN2688972.

Gene expression databases

GenevestigatorQ8L500.

Family and domain databases

InterProIPR010402. CCT_domain.
IPR011006. CheY-like_superfamily.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamPF06203. CCT. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
SMARTSM00448. REC. 1 hit.
[Graphical view]
SUPFAMSSF52172. SSF52172. 1 hit.
PROSITEPS51017. CCT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPRR9_ARATH
AccessionPrimary (citable) accession number: Q8L500
Secondary accession number(s): O81035, Q9LKL1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: April 16, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names