Q8L500 (APRR9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Two-component response regulator-like APRR9

Alternative name(s):
Pseudo-response regulator 9

Gene names

Name:	APRR9
Ordered Locus Names:	At2g46790
ORF Names:	F19D11.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Controls photoperiodic flowering response. Seems to be one of the component of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. Ref.1
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the ARR-like family. Contains 1 CCT domain. Contains 1 response regulatory domain.
Caution	Lacks the phospho-accepting Asp (here Glu-89), present in the receiver domain, which is one of the conserved features of two-component response regulators (ARRs) family.
Sequence caution	The sequence AAM20527.1 differs from that shown. Reason: Frameshift at position 117. The sequence AAM91256.1 differs from that shown. Reason: Frameshift at position 117.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	circadian rhythm Inferred from mutant phenotype PubMed 15767265. Source: TAIR intracellular signal transduction Inferred from electronic annotation. Source: GOC negative regulation of transcription, DNA-dependent Inferred from direct assay PubMed 20233950. Source: TAIR red or far-red light signaling pathway Inferred from mutant phenotype PubMed 16891401. Source: TAIR response to temperature stimulus Inferred from genetic interaction PubMed 15767265. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from physical interaction PubMed 20233950. Source: TAIR phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8L500-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Two-component response regulator-like APRR9		PRO_0000081441
Regions
	Domain	38 – 156	119	Response regulatory
	Domain	417 – 459	43	CCT

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 27, 2004. Version 2. Checksum: 19D6C9571CA77A40	FASTA	468	52,565
	10 20 30 40 50 60 MGEIVVLSSD DGMETIKNRV KSSEVVQWEK YLPKTVLRVL LVESDYSTRQ IITALLRKCC 70 80 90 100 110 120 YKVVAVSDGL AAWEVLKEKS HNIDLILTEL DLPSISGFAL LALVMEHEAC KNIPVIMMSS 130 140 150 160 170 180 QDSIKMVLKC MLRGAADYLI KPMRKNELKN LWQHVWRRLT LRDDPTAHAQ SLPASQHNLE 190 200 210 220 230 240 DTDETCEDSR YHSDQGSGAQ AINYNGHNKL MENGKSVDER DEFKETFDVT MDLIGGIDKR 250 260 270 280 290 300 PDSIYKDKSR DECVGPELGL SLKRSCSVSF ENQDESKHQK LSLSDASAFS RFEESKSAEK 310 320 330 340 350 360 AVVALEESTS GEPKTPTESH EKLRKVTSDQ GSATTSSNQE NIGSSSVSFR NQVLQSTVTN 370 380 390 400 410 420 QKQDSPIPVE SNREKAASKE VEAGSQSTNE GIAGQSSSTE KPKEEESAKQ RWSRSQREAA 430 440 450 460 LMKFRLKRKD RCFDKKVRYQ SRKKLAEQRP RVKGQFVRTV NSDASTKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana: insight into the plant circadian clock." Matsushika A., Makino S., Kojima M., Mizuno T. Plant Cell Physiol. 41:1002-1012(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, FUNCTION.
[2]	"Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog." Strayer C., Oyama T., Schultz T.F., Raman R., Somers D.E., Mas P., Panda S., Kreps J.A., Kay S.A. Science 289:768-771(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana." Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T., Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T. Plant Cell Physiol. 41:791-803(2000) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB046953 mRNA. Translation: BAB13741.1. AF272040 mRNA. Translation: AAF86253.1. AC005310 Genomic DNA. Translation: AAC33497.2. CP002685 Genomic DNA. Translation: AEC10754.1. AY099676 mRNA. Translation: AAM20527.1. Frameshift. AY128856 mRNA. Translation: AAM91256.1. Frameshift.
IPI	IPI00545462.
PIR	T02680.
RefSeq	NP_566085.1. NM_130245.4.
UniGene	At.1675. At.65052.
3D structure databases
ProteinModelPortal	Q8L500.
SMR	Q8L500. Positions 39-200.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-7908741.
Proteomic databases
PRIDE	Q8L500.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G46790.1; AT2G46790.1; AT2G46790.
GeneID	819292.
KEGG	ath:AT2G46790.
Organism-specific databases
GeneFarm	4360. 437.
TAIR	At2g46790.
Phylogenomic databases
eggNOG	COG0745.
HOGENOM	HOG000034018.
InParanoid	Q8L500.
KO	K12128.
OMA	HEACKNI.
PhylomeDB	Q8L500.
ProtClustDB	CLSN2688972.
Gene expression databases
ArrayExpress	Q8L500.
Genevestigator	Q8L500.
GermOnline	AT2G46790. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR010402. CCT_domain. IPR011006. CheY-like_superfamily. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Pfam	PF06203. CCT. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52172. CheY_like. 1 hit.
PROSITE	PS51017. CCT. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APRR9_ARATH

Accession

Primary (citable) accession number: Q8L500
Secondary accession number(s): O81035, Q9LKL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	September 27, 2004
Last modified:	May 29, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program