Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8L500

- APRR9_ARATH

UniProt

Q8L500 - APRR9_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Two-component response regulator-like APRR9

Gene

APRR9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional repressor of CCA1 and LHY, and positive regulator of LWD1 and LWD2 expression. Controls photoperiodic flowering response and temperature compensation. Involved in the positive and negative feedback loops of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. Regulated at the transcriptional level by a corepressor complex consisting of ELF4, ELF3, and LUX. APRR9, APRR7, and APRR5 coordinately act on the upstream region of the target genes to repress their expression from noon until midnight. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock.5 Publications

GO - Molecular functioni

  1. DNA binding Source: TAIR

GO - Biological processi

  1. circadian rhythm Source: TAIR
  2. negative regulation of transcription, DNA-templated Source: TAIR
  3. phosphorelay signal transduction system Source: UniProtKB-KW
  4. red or far-red light signaling pathway Source: TAIR
  5. regulation of transcription, DNA-templated Source: TAIR
  6. response to temperature stimulus Source: TAIR
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation, Two-component regulatory system

Names & Taxonomyi

Protein namesi
Recommended name:
Two-component response regulator-like APRR9
Alternative name(s):
Pseudo-response regulator 9
Gene namesi
Name:APRR9
Ordered Locus Names:At2g46790
ORF Names:F19D11.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G46790.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Two-component response regulator-like APRR9PRO_0000081441Add
BLAST

Post-translational modificationi

Phosphorylated. Phosphorylation varies throughout the diurnal cycle.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ8L500.

Expressioni

Inductioni

Up-regulated by heat-shock.2 Publications

Gene expression databases

ExpressionAtlasiQ8L500. baseline and differential.
GenevestigatoriQ8L500.

Interactioni

Protein-protein interaction databases

BioGridi4627. 6 interactions.
IntActiQ8L500. 1 interaction.
MINTiMINT-7908741.

Structurei

3D structure databases

ProteinModelPortaliQ8L500.
SMRiQ8L500. Positions 39-200.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 156119Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini417 – 45943CCTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ARR-like family.Curated
Contains 1 CCT domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034018.
InParanoidiQ8L500.
KOiK12128.
OMAiHEACKNI.
PhylomeDBiQ8L500.

Family and domain databases

InterProiIPR010402. CCT_domain.
IPR011006. CheY-like_superfamily.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF06203. CCT. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS51017. CCT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8L500-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEIVVLSSD DGMETIKNRV KSSEVVQWEK YLPKTVLRVL LVESDYSTRQ
60 70 80 90 100
IITALLRKCC YKVVAVSDGL AAWEVLKEKS HNIDLILTEL DLPSISGFAL
110 120 130 140 150
LALVMEHEAC KNIPVIMMSS QDSIKMVLKC MLRGAADYLI KPMRKNELKN
160 170 180 190 200
LWQHVWRRLT LRDDPTAHAQ SLPASQHNLE DTDETCEDSR YHSDQGSGAQ
210 220 230 240 250
AINYNGHNKL MENGKSVDER DEFKETFDVT MDLIGGIDKR PDSIYKDKSR
260 270 280 290 300
DECVGPELGL SLKRSCSVSF ENQDESKHQK LSLSDASAFS RFEESKSAEK
310 320 330 340 350
AVVALEESTS GEPKTPTESH EKLRKVTSDQ GSATTSSNQE NIGSSSVSFR
360 370 380 390 400
NQVLQSTVTN QKQDSPIPVE SNREKAASKE VEAGSQSTNE GIAGQSSSTE
410 420 430 440 450
KPKEEESAKQ RWSRSQREAA LMKFRLKRKD RCFDKKVRYQ SRKKLAEQRP
460
RVKGQFVRTV NSDASTKS
Length:468
Mass (Da):52,565
Last modified:September 27, 2004 - v2
Checksum:i19D6C9571CA77A40
GO

Sequence cautioni

The sequence AAM20527.1 differs from that shown. Reason: Frameshift at position 117. Curated
The sequence AAM91256.1 differs from that shown. Reason: Frameshift at position 117. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046953 mRNA. Translation: BAB13741.1.
AF272040 mRNA. Translation: AAF86253.1.
AC005310 Genomic DNA. Translation: AAC33497.2.
CP002685 Genomic DNA. Translation: AEC10754.1.
AY099676 mRNA. Translation: AAM20527.1. Frameshift.
AY128856 mRNA. Translation: AAM91256.1. Frameshift.
PIRiT02680.
RefSeqiNP_566085.1. NM_130245.4. [Q8L500-1]
UniGeneiAt.1675.
At.65052.

Genome annotation databases

EnsemblPlantsiAT2G46790.1; AT2G46790.1; AT2G46790. [Q8L500-1]
GeneIDi819292.
KEGGiath:AT2G46790.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046953 mRNA. Translation: BAB13741.1 .
AF272040 mRNA. Translation: AAF86253.1 .
AC005310 Genomic DNA. Translation: AAC33497.2 .
CP002685 Genomic DNA. Translation: AEC10754.1 .
AY099676 mRNA. Translation: AAM20527.1 . Frameshift.
AY128856 mRNA. Translation: AAM91256.1 . Frameshift.
PIRi T02680.
RefSeqi NP_566085.1. NM_130245.4. [Q8L500-1 ]
UniGenei At.1675.
At.65052.

3D structure databases

ProteinModelPortali Q8L500.
SMRi Q8L500. Positions 39-200.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4627. 6 interactions.
IntActi Q8L500. 1 interaction.
MINTi MINT-7908741.

Proteomic databases

PRIDEi Q8L500.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G46790.1 ; AT2G46790.1 ; AT2G46790 . [Q8L500-1 ]
GeneIDi 819292.
KEGGi ath:AT2G46790.

Organism-specific databases

GeneFarmi 4360. 437.
TAIRi AT2G46790.

Phylogenomic databases

eggNOGi COG0745.
HOGENOMi HOG000034018.
InParanoidi Q8L500.
KOi K12128.
OMAi HEACKNI.
PhylomeDBi Q8L500.

Gene expression databases

ExpressionAtlasi Q8L500. baseline and differential.
Genevestigatori Q8L500.

Family and domain databases

InterProi IPR010402. CCT_domain.
IPR011006. CheY-like_superfamily.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view ]
Pfami PF06203. CCT. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view ]
SMARTi SM00448. REC. 1 hit.
[Graphical view ]
SUPFAMi SSF52172. SSF52172. 1 hit.
PROSITEi PS51017. CCT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana: insight into the plant circadian clock."
    Matsushika A., Makino S., Kojima M., Mizuno T.
    Plant Cell Physiol. 41:1002-1012(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog."
    Strayer C., Oyama T., Schultz T.F., Raman R., Somers D.E., Mas P., Panda S., Kreps J.A., Kay S.A.
    Science 289:768-771(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana."
    Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T., Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T.
    Plant Cell Physiol. 41:791-803(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  7. "Post-translational regulation of the Arabidopsis circadian clock through selective proteolysis and phosphorylation of pseudo-response regulator proteins."
    Fujiwara S., Wang L., Han L., Suh S.-S., Salome P.A., McClung C.R., Somers D.E.
    J. Biol. Chem. 283:23073-23083(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  8. Cited for: REGULATION.
  9. "PSEUDO-RESPONSE REGULATORS 9, 7, and 5 are transcriptional repressors in the Arabidopsis circadian clock."
    Nakamichi N., Kiba T., Henriques R., Mizuno T., Chua N.H., Sakakibara H.
    Plant Cell 22:594-605(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "The role of the Arabidopsis morning loop components CCA1, LHY, PRR7, and PRR9 in temperature compensation."
    Salome P.A., Weigel D., McClung C.R.
    Plant Cell 22:3650-3661(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "LIGHT-REGULATED WD1 and PSEUDO-RESPONSE REGULATOR9 form a positive feedback regulatory loop in the Arabidopsis circadian clock."
    Wang Y., Wu J.F., Nakamichi N., Sakakibara H., Nam H.G., Wu S.H.
    Plant Cell 23:486-498(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  12. "ELF3 recruitment to the PRR9 promoter requires other Evening Complex members in the Arabidopsis circadian clock."
    Chow B.Y., Helfer A., Nusinow D.A., Kay S.A.
    Plant Signal. Behav. 7:170-173(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION.
  13. "EARLY FLOWERING4 recruitment of EARLY FLOWERING3 in the nucleus sustains the Arabidopsis circadian clock."
    Herrero E., Kolmos E., Bujdoso N., Yuan Y., Wang M., Berns M.C., Uhlworm H., Coupland G., Saini R., Jaskolski M., Webb A., Goncalves J., Davis S.J.
    Plant Cell 24:428-443(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION.
  14. "SKIP is a component of the spliceosome linking alternative splicing and the circadian clock in Arabidopsis."
    Wang X., Wu F., Xie Q., Wang H., Wang Y., Yue Y., Gahura O., Ma S., Liu L., Cao Y., Jiao Y., Puta F., McClung C.R., Xu X., Ma L.
    Plant Cell 24:3278-3295(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION.
  15. Cited for: FUNCTION.
  16. "Heat shock-induced fluctuations in clock and light signaling enhance phytochrome B-mediated Arabidopsis deetiolation."
    Karayekov E., Sellaro R., Legris M., Yanovsky M.J., Casal J.J.
    Plant Cell 25:2892-2906(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HEAT-SHOCK.

Entry informationi

Entry nameiAPRR9_ARATH
AccessioniPrimary (citable) accession number: Q8L500
Secondary accession number(s): O81035, Q9LKL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: October 29, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Regulated at the level of mRNA maturation and alternative splicing by SKIP (PubMed:22942380) and PRMT5 (PubMed:20962777). The expression of APRR9, APRR7, and APRR5 requires the presence of LWD1 and/or LWD2, indicating the existence of a positive feedback loop within the circadian clock (PubMed:21357491).3 Publications

Caution

Lacks the phospho-accepting Asp (here Glu-89), present in the receiver domain, which is one of the conserved features of two-component response regulators (ARRs) family.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3