ID   LPAT4_ARATH             Reviewed;         378 AA.
AC   Q8L4Y2; Q9C9P8; Q9SSH0;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 38.
DE   RecName: Full=Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4;
DE            EC=2.3.1.51;
DE   AltName: Full=Lysophosphatidyl acyltransferase 4;
GN   Name=LPAT4; Synonyms=LPAAT4; OrderedLocusNames=At1g75020;
GN   ORFNames=F25A4.2;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   TISSUE SPECIFICITY.
RX   PubMed=15772283; DOI=10.1105/tpc.104.030403;
RA   Kim H.U., Li Y., Huang A.H.C.;
RT   "Ubiquitous and endoplasmic reticulum-located lysophosphatidyl
RT   acyltransferase, LPAT2, is essential for female but not male
RT   gametophyte development in Arabidopsis.";
RL   Plant Cell 17:1073-1089(2005).
CC   -!- FUNCTION: May convert lysophosphatidic acid (LPA) into
CC       phosphatidic acid by incorporating acyl moiety at the 2 position
CC       (By similarity). Has no activity when expressed in bacteria or
CC       yeast.
CC   -!- CATALYTIC ACTIVITY: Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate =
CC       CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
CC   -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis;
CC       CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
CC       (Potential).
CC   -!- TISSUE SPECIFICITY: Widely expressed at low level.
CC   -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity
CC       and may constitute the binding site for the phosphate moiety of
CC       the glycerol-3-phosphate (By similarity).
CC   -!- SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate
CC       acyltransferase family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD55275.1; Type=Erroneous gene model prediction;
CC       Sequence=AAG51931.1; Type=Erroneous gene model prediction;
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DR   EMBL; AC008263; AAD55275.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AC013258; AAG51931.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AY099547; AAM20399.1; -; mRNA.
DR   EMBL; BT001236; AAN65123.1; -; mRNA.
DR   EMBL; AY084709; AAM61283.1; -; mRNA.
DR   IPI; IPI00537505; -.
DR   PIR; B96780; B96780.
DR   RefSeq; NP_565098.1; -.
DR   RefSeq; NP_974144.1; -.
DR   UniGene; At.43179; -.
DR   GeneID; 843840; -.
DR   GenomeReviews; CT485782_GR; AT1G75020.
DR   KEGG; ath:AT1G75020; -.
DR   NMPDR; fig|3702.1.peg.7018; -.
DR   TAIR; At1g75020; -.
DR   OMA; Q8L4Y2; ITIAYKH.
DR   BRENDA; 2.3.1.51; 302.
DR   ArrayExpress; Q8L4Y2; -.
DR   GermOnline; AT1G75020; Arabidopsis thaliana.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0003841; F:1-acylglycerol-3-phosphate O-acyltransferas...; IEA:EC.
DR   GO; GO:0008654; P:phospholipid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR002123; Acyltransferase.
DR   Pfam; PF01553; Acyltransferase; 1.
DR   SMART; SM00563; PlsC; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Complete proteome; Membrane;
KW   Phospholipid biosynthesis; Transferase; Transmembrane.
FT   CHAIN         1    378       Probable 1-acyl-sn-glycerol-3-phosphate
FT                                acyltransferase 4.
FT                                /FTId=PRO_0000208183.
FT   TRANSMEM     26     46       Potential.
FT   TRANSMEM     62     82       Potential.
FT   TRANSMEM    317    337       Potential.
FT   MOTIF       105    110       HXXXXD motif.
SQ   SEQUENCE   378 AA;  43064 MW;  36DB41D29E3B7ABC CRC64;
     MEVCGDLKSD NLKNRPLTPL RILRGLMILL VFLSTAFMFL LYFAPIAALG LRLLSVQQSR
     KVVSLIFGLW LALWPYLFET VNGTTVVFSG DIIPVEKRVL LIANHRTEVD WMYLWNIALR
     KGCLGYIKYV LKSSLMKLPI FGWGFHVLEF IPVERKREVD EPVLLQMLSS FKDPQEPLWL
     ALFPEGTDFT EEKCKRSQKF AAEVGLPALS NVLLPKTRGF GVCLEVLHNS LDAVYDLTIA
     YKPRCPSFMD NVFGTDPSEV HIHVRRVLLK EIPANEAESS AWLMDSFKLK DKLLSDFNAQ
     GKFPNQRPEE ELSVLKCIAT FAGVISLTVV FIYLTLYSHS CFKVYACLSG TYLTFATYYK
     FQPSPGCFRE DSCKVKNH
//