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Protein

Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4

Gene

LPAT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May convert lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position (By similarity). Has no activity when expressed in bacteria or yeast.By similarity

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Probable glycerol-3-phosphate acyltransferase 2 (GPAT2), Glycerol-3-phosphate acyltransferase, chloroplastic (ATS1), Glycerol-3-phosphate acyltransferase 1 (GPAT1), Probable glycerol-3-phosphate acyltransferase 3 (GPAT3), Glycerol-3-phosphate acyltransferase 7 (GPAT7), Probable glycerol-3-phosphate acyltransferase 8 (GPAT8), Glycerol-3-phosphate acyltransferase 5 (GPAT5)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase 3 (LPAT3), 1-acyl-sn-glycerol-3-phosphate acyltransferase 2 (LPAT2), 1-acyl-sn-glycerol-3-phosphate acyltransferase 1, chloroplastic (LPAT1), Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 (LPAT5), Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4 (LPAT4)
  3. Phosphatidate cytidylyltransferase 1 (CDS1), Phosphatidate cytidylyltransferase 3 (CDS3), Phosphatidate cytidylyltransferase 4, chloroplastic (CDS4), Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase 5, chloroplastic (CDS5), Phosphatidate cytidylyltransferase (CDS2), Phosphatidate cytidylyltransferase (CDS2)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciARA:AT1G75020-MONOMER.
ARA:GQT-2611-MONOMER.
BRENDAi2.3.1.51. 399.
ReactomeiR-ATH-1482798. Acyl chain remodeling of CL.
R-ATH-1483166. Synthesis of PA.
R-ATH-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00557; UER00613.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4 (EC:2.3.1.51)
Alternative name(s):
Lysophosphatidyl acyltransferase 4
Gene namesi
Name:LPAT4
Synonyms:LPAAT4
Ordered Locus Names:At1g75020
ORF Names:F25A4.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G75020.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei26 – 4621HelicalSequence analysisAdd
BLAST
Transmembranei62 – 8221HelicalSequence analysisAdd
BLAST
Transmembranei317 – 33721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 378378Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4PRO_0000208183Add
BLAST

Proteomic databases

PaxDbiQ8L4Y2.
PRIDEiQ8L4Y2.

Expressioni

Tissue specificityi

Widely expressed at low level.1 Publication

Gene expression databases

GenevisibleiQ8L4Y2. AT.

Interactioni

Protein-protein interaction databases

BioGridi29059. 9 interactions.
IntActiQ8L4Y2. 1 interaction.
STRINGi3702.AT1G75020.1.

Structurei

3D structure databases

ProteinModelPortaliQ8L4Y2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi105 – 1106HXXXXD motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1505. Eukaryota.
COG0204. LUCA.
HOGENOMiHOG000005662.
InParanoidiQ8L4Y2.
KOiK13513.
OMAiRDSHISH.
PhylomeDBiQ8L4Y2.

Family and domain databases

InterProiIPR032098. Acyltransf_C.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF16076. Acyltransf_C. 1 hit.
PF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8L4Y2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVCGDLKSD NLKNRPLTPL RILRGLMILL VFLSTAFMFL LYFAPIAALG
60 70 80 90 100
LRLLSVQQSR KVVSLIFGLW LALWPYLFET VNGTTVVFSG DIIPVEKRVL
110 120 130 140 150
LIANHRTEVD WMYLWNIALR KGCLGYIKYV LKSSLMKLPI FGWGFHVLEF
160 170 180 190 200
IPVERKREVD EPVLLQMLSS FKDPQEPLWL ALFPEGTDFT EEKCKRSQKF
210 220 230 240 250
AAEVGLPALS NVLLPKTRGF GVCLEVLHNS LDAVYDLTIA YKPRCPSFMD
260 270 280 290 300
NVFGTDPSEV HIHVRRVLLK EIPANEAESS AWLMDSFKLK DKLLSDFNAQ
310 320 330 340 350
GKFPNQRPEE ELSVLKCIAT FAGVISLTVV FIYLTLYSHS CFKVYACLSG
360 370
TYLTFATYYK FQPSPGCFRE DSCKVKNH
Length:378
Mass (Da):43,064
Last modified:October 1, 2002 - v1
Checksum:i36DB41D29E3B7ABC
GO

Sequence cautioni

The sequence AAD55275.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAG51931.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008263 Genomic DNA. Translation: AAD55275.1. Sequence problems.
AC013258 Genomic DNA. Translation: AAG51931.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35662.1.
CP002684 Genomic DNA. Translation: AEE35663.1.
AY099547 mRNA. Translation: AAM20399.1.
BT001236 mRNA. Translation: AAN65123.1.
AY084709 mRNA. Translation: AAM61283.1.
PIRiB96780.
RefSeqiNP_565098.1. NM_106159.2.
NP_974144.1. NM_202415.1.
UniGeneiAt.43179.

Genome annotation databases

EnsemblPlantsiAT1G75020.1; AT1G75020.1; AT1G75020.
AT1G75020.2; AT1G75020.2; AT1G75020.
GeneIDi843840.
GrameneiAT1G75020.1; AT1G75020.1; AT1G75020.
AT1G75020.2; AT1G75020.2; AT1G75020.
KEGGiath:AT1G75020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008263 Genomic DNA. Translation: AAD55275.1. Sequence problems.
AC013258 Genomic DNA. Translation: AAG51931.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35662.1.
CP002684 Genomic DNA. Translation: AEE35663.1.
AY099547 mRNA. Translation: AAM20399.1.
BT001236 mRNA. Translation: AAN65123.1.
AY084709 mRNA. Translation: AAM61283.1.
PIRiB96780.
RefSeqiNP_565098.1. NM_106159.2.
NP_974144.1. NM_202415.1.
UniGeneiAt.43179.

3D structure databases

ProteinModelPortaliQ8L4Y2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29059. 9 interactions.
IntActiQ8L4Y2. 1 interaction.
STRINGi3702.AT1G75020.1.

Proteomic databases

PaxDbiQ8L4Y2.
PRIDEiQ8L4Y2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G75020.1; AT1G75020.1; AT1G75020.
AT1G75020.2; AT1G75020.2; AT1G75020.
GeneIDi843840.
GrameneiAT1G75020.1; AT1G75020.1; AT1G75020.
AT1G75020.2; AT1G75020.2; AT1G75020.
KEGGiath:AT1G75020.

Organism-specific databases

TAIRiAT1G75020.

Phylogenomic databases

eggNOGiKOG1505. Eukaryota.
COG0204. LUCA.
HOGENOMiHOG000005662.
InParanoidiQ8L4Y2.
KOiK13513.
OMAiRDSHISH.
PhylomeDBiQ8L4Y2.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00613.
BioCyciARA:AT1G75020-MONOMER.
ARA:GQT-2611-MONOMER.
BRENDAi2.3.1.51. 399.
ReactomeiR-ATH-1482798. Acyl chain remodeling of CL.
R-ATH-1483166. Synthesis of PA.
R-ATH-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ8L4Y2.

Gene expression databases

GenevisibleiQ8L4Y2. AT.

Family and domain databases

InterProiIPR032098. Acyltransf_C.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF16076. Acyltransf_C. 1 hit.
PF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Ubiquitous and endoplasmic reticulum-located lysophosphatidyl acyltransferase, LPAT2, is essential for female but not male gametophyte development in Arabidopsis."
    Kim H.U., Li Y., Huang A.H.C.
    Plant Cell 17:1073-1089(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiLPAT4_ARATH
AccessioniPrimary (citable) accession number: Q8L4Y2
Secondary accession number(s): Q9C9P8, Q9SSH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: October 1, 2002
Last modified: February 17, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.