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Protein

Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4

Gene

LPAT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May convert lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position (By similarity). Has no activity when expressed in bacteria or yeast.By similarity

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase, chloroplastic (ATS1), Glycerol-3-phosphate acyltransferase 1 (GPAT1), Probable glycerol-3-phosphate acyltransferase 3 (GPAT3), Glycerol-3-phosphate acyltransferase 5 (GPAT5), Glycerol-3-phosphate acyltransferase, chloroplastic (AXX17_At1g32940), Probable glycerol-3-phosphate acyltransferase 2 (GPAT2), Glycerol-3-phosphate acyltransferase 7 (GPAT7), Probable glycerol-3-phosphate acyltransferase 8 (GPAT8)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase 3 (LPAT3), 1-acyl-sn-glycerol-3-phosphate acyltransferase 1, chloroplastic (LPAT1), Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 (LPAT5), Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4 (LPAT4), 1-acyl-sn-glycerol-3-phosphate acyltransferase 2 (LPAT2)
  3. Phosphatidate cytidylyltransferase 1 (CDS1), Phosphatidate cytidylyltransferase (AXX17_At1g55730), Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase (AXX17_At2g42720), Phosphatidate cytidylyltransferase (AXX17_At4g30920), Phosphatidate cytidylyltransferase (CDS2), Phosphatidate cytidylyltransferase (CDS2), Phosphatidate cytidylyltransferase 3 (CDS3), Phosphatidate cytidylyltransferase 4, chloroplastic (CDS4), Phosphatidate cytidylyltransferase (AXX17_At3g55010), Phosphatidate cytidylyltransferase (AXX17_At4g25970), Phosphatidate cytidylyltransferase 5, chloroplastic (CDS5)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciARA:AT1G75020-MONOMER
BRENDAi2.3.1.51 399
ReactomeiR-ATH-1482798 Acyl chain remodeling of CL
R-ATH-1483166 Synthesis of PA
UniPathwayiUPA00557; UER00613

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4 (EC:2.3.1.51)
Alternative name(s):
Lysophosphatidyl acyltransferase 4
Gene namesi
Name:LPAT4
Synonyms:LPAAT4
Ordered Locus Names:At1g75020
ORF Names:F25A4.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G75020
TAIRilocus:2027196 AT1G75020

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei26 – 46HelicalSequence analysisAdd BLAST21
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Transmembranei317 – 337HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002081831 – 378Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4Add BLAST378

Proteomic databases

PaxDbiQ8L4Y2
PRIDEiQ8L4Y2

Expressioni

Tissue specificityi

Widely expressed at low level.1 Publication

Gene expression databases

ExpressionAtlasiQ8L4Y2 baseline and differential
GenevisibleiQ8L4Y2 AT

Interactioni

Protein-protein interaction databases

BioGridi29059, 9 interactors
IntActiQ8L4Y2, 1 interactor
STRINGi3702.AT1G75020.1

Structurei

3D structure databases

ProteinModelPortaliQ8L4Y2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi105 – 110HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1505 Eukaryota
COG0204 LUCA
HOGENOMiHOG000005662
InParanoidiQ8L4Y2
KOiK13513
OMAiESSAWLM
OrthoDBiEOG09360ABC
PhylomeDBiQ8L4Y2

Family and domain databases

InterProiView protein in InterPro
IPR032098 Acyltransf_C
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF16076 Acyltransf_C, 1 hit
PF01553 Acyltransferase, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit

Sequencei

Sequence statusi: Complete.

Q8L4Y2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVCGDLKSD NLKNRPLTPL RILRGLMILL VFLSTAFMFL LYFAPIAALG
60 70 80 90 100
LRLLSVQQSR KVVSLIFGLW LALWPYLFET VNGTTVVFSG DIIPVEKRVL
110 120 130 140 150
LIANHRTEVD WMYLWNIALR KGCLGYIKYV LKSSLMKLPI FGWGFHVLEF
160 170 180 190 200
IPVERKREVD EPVLLQMLSS FKDPQEPLWL ALFPEGTDFT EEKCKRSQKF
210 220 230 240 250
AAEVGLPALS NVLLPKTRGF GVCLEVLHNS LDAVYDLTIA YKPRCPSFMD
260 270 280 290 300
NVFGTDPSEV HIHVRRVLLK EIPANEAESS AWLMDSFKLK DKLLSDFNAQ
310 320 330 340 350
GKFPNQRPEE ELSVLKCIAT FAGVISLTVV FIYLTLYSHS CFKVYACLSG
360 370
TYLTFATYYK FQPSPGCFRE DSCKVKNH
Length:378
Mass (Da):43,064
Last modified:October 1, 2002 - v1
Checksum:i36DB41D29E3B7ABC
GO

Sequence cautioni

The sequence AAD55275 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG51931 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008263 Genomic DNA Translation: AAD55275.1 Sequence problems.
AC013258 Genomic DNA Translation: AAG51931.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE35662.1
CP002684 Genomic DNA Translation: AEE35663.1
AY099547 mRNA Translation: AAM20399.1
BT001236 mRNA Translation: AAN65123.1
AY084709 mRNA Translation: AAM61283.1
PIRiB96780
RefSeqiNP_565098.1, NM_106159.3
NP_974144.1, NM_202415.2
UniGeneiAt.43179

Genome annotation databases

EnsemblPlantsiAT1G75020.1; AT1G75020.1; AT1G75020
AT1G75020.2; AT1G75020.2; AT1G75020
GeneIDi843840
GrameneiAT1G75020.1; AT1G75020.1; AT1G75020
AT1G75020.2; AT1G75020.2; AT1G75020
KEGGiath:AT1G75020

Similar proteinsi

Entry informationi

Entry nameiLPAT4_ARATH
AccessioniPrimary (citable) accession number: Q8L4Y2
Secondary accession number(s): Q9C9P8, Q9SSH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: October 1, 2002
Last modified: April 25, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health