Wee1-like protein kinase - Arabidopsis thaliana (Mouse-ear cress)

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Q8L4H0 (WEE1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 81. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Wee1-like protein kinase
EC=2.7.10.2

Alternative name(s):
Wee1-At

Gene names

Name:	WEE1
Ordered Locus Names:	At1g02970
ORF Names:	F22D16.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell cycle regulatory kinase that is not rate-limiting for cycle progression under normal growth conditions. Transcriptionally activated upon DNA stress or damage in an ATR- or ATM-dependent manner. Once activated, inhibits plant growth by arresting dividing cells in the G2 phase before proceeding into mitosis. Down-regulates CDKA-1 and CDKD-2 by tyrosine phosphorylation. May target principally CDKA-1. Ref.1 Ref.2 Ref.5
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with CDKA-1, but not with CDKB1-1. Ref.5
Subcellular location	Nucleus Ref.2.
Tissue specificity	Expressed in shoot apex, vasculatures tissues of roots and leaves, and developing flowers. Ref.1 Ref.5
Induction	By replication blocking agents (hydroxyurea and aphidicolin). Ref.5
Disruption phenotype	Plants show no obvious cell division or endoreduplication phenotype when grown under nonstress conditions, but are hypersensitive to agents that impair DNA replication. Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAF02869.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication checkpoint Inferred from expression pattern Ref.5. Source: TAIR cell cycle arrest Inferred from expression pattern Ref.5. Source: TAIR cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein kinase activity Inferred from direct assay Ref.2. Source: TAIR protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 500

500

Wee1-like protein kinase

PRO_0000295672

Regions

Domain

249 – 495

247

Protein kinase

Nucleotide binding

255 – 263

ATP By similarity

Sites

Active site

372

Proton acceptor By similarity

Metal binding

377

Magnesium; via carbonyl oxygen By similarity

Metal binding

389

Magnesium; via carbonyl oxygen By similarity

Binding site

278

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8L4H0 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 7E7C32697B9A9E44
Show »

FASTA

500

56,530

        10         20         30         40         50         60 
MFEKNGRTLL AKRKTQGTIK TRASKKIRKM EGTLERHSLL QFGQLSKISF ENRPSSNVAS 

        70         80         90        100        110        120 
SAFQGLLDSD SSELRNQLGS ADSDANCGEK DFILSQDFFC TPDYITPDNQ NLMSGLDISK 

       130        140        150        160        170        180 
DHSPCPRSPV KLNTVKSKRC RQESFTGNHS NSTWSSKHRV DEQENDDIDT DEVMGDKLQA 

       190        200        210        220        230        240 
NQTERTGYVS QAAVALRCRA MPPPCLKNPY VLNQSETATD PFGHQRSKCA SFLPVSTSGD 

       250        260        270        280        290        300 
GLSRYLTDFH EIRQIGAGHF SRVFKVLKRM DGCLYAVKHS TRKLYLDSER RKAMMEVQAL 

       310        320        330        340        350        360 
AALGFHENIV GYYSSWFENE QLYIQLELCD HSLSALPKKS SLKVSEREIL VIMHQIAKAL 

       370        380        390        400        410        420 
HFVHEKGIAH LDVKPDNIYI KNGVCKLGDF GCATRLDKSL PVEEGDARYM PQEILNEDYE 

       430        440        450        460        470        480 
HLDKVDIFSL GVTVYELIKG SPLTESRNQS LNIKEGKLPL LPGHSLQLQQ LLKTMMDRDP 

       490        500 
KRRPSARELL DHPMFDRIRG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A WEE1 homologue from Arabidopsis thaliana." Sorrell D.A., Marchbank A., McMahon K., Dickinson J.R., Rogers H.J., Francis D. Planta 215:518-522(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Diverse phosphoregulatory mechanisms controlling cyclin-dependent kinase-activating kinases in Arabidopsis." Shimotohno A., Ohno R., Bisova K., Sakaguchi N., Huang J., Koncz C., Uchimiya H., Umeda M. Plant J. 47:701-710(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Arabidopsis WEE1 kinase controls cell cycle arrest in response to activation of the DNA integrity checkpoint." de Schutter K., Joubes J., Cools T., Verkest A., Corellou F., Babiychuk E., van der Schueren E., Beeckman T., Kushnir S., Inze D., de Veylder L. Plant Cell 19:211-225(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION, INTERACTION WITH CDKA-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB077385 mRNA. Translation: BAC11716.1. AJ439593 mRNA. Translation: CAD28679.1. AC009525 Genomic DNA. Translation: AAF02869.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE27508.1.
IPI	IPI00535347.
PIR	C86160.
RefSeq	NP_171796.1. NM_100178.2.
UniGene	At.42570.
3D structure databases
HSSP	HSSP built from PDB template 2A19 based on UniProtKB P19525.
ProteinModelPortal	Q8L4H0.
SMR	Q8L4H0. Positions 243-495.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8L4H0. 8 interactions.
STRING	3702.AT1G02970.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G02970.1; AT1G02970.1; AT1G02970.
GeneID	839453.
KEGG	ath:AT1G02970.
Organism-specific databases
GeneFarm	3422.
TAIR	At1g02970.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000083236.
InParanoid	Q8L4H0.
KO	K06632.
OMA	LLYQICK.
PhylomeDB	Q8L4H0.
ProtClustDB	CLSN2679628.
Gene expression databases
Genevestigator	Q8L4H0.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WEE1_ARATH

Accession

Primary (citable) accession number: Q8L4H0
Secondary accession number(s): Q9SRY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents