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Protein

Wee1-like protein kinase

Gene

WEE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell cycle regulatory kinase that is not rate-limiting for cycle progression under normal growth conditions. Transcriptionally activated upon DNA stress or damage in an ATR- or ATM-dependent manner. Once activated, inhibits plant growth by arresting dividing cells in the G2 phase before proceeding into mitosis. Down-regulates CDKA-1 and CDKD-2 by tyrosine phosphorylation. May target principally CDKA-1.3 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei278ATPPROSITE-ProRule annotation1
Active sitei372Proton acceptorPROSITE-ProRule annotation1
Metal bindingi377Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi389Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi255 – 263ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell cycle arrest Source: TAIR
  • cell division Source: UniProtKB-KW
  • DNA replication checkpoint Source: TAIR
  • mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-69656. Cyclin A:Cdk2-associated events at S phase entry.

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase (EC:2.7.10.2)
Alternative name(s):
Wee1-At
Gene namesi
Name:WEE1
Ordered Locus Names:At1g02970
ORF Names:F22D16.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G02970.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Plants show no obvious cell division or endoreduplication phenotype when grown under nonstress conditions, but are hypersensitive to agents that impair DNA replication.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002956721 – 500Wee1-like protein kinaseAdd BLAST500

Proteomic databases

PaxDbiQ8L4H0.

Expressioni

Tissue specificityi

Expressed in shoot apex, vasculatures tissues of roots and leaves, and developing flowers.2 Publications

Inductioni

By replication blocking agents (hydroxyurea and aphidicolin).1 Publication

Gene expression databases

GenevisibleiQ8L4H0. AT.

Interactioni

Subunit structurei

Interacts with CDKA-1, but not with CDKB1-1.1 Publication

Protein-protein interaction databases

BioGridi24688. 8 interactors.
IntActiQ8L4H0. 8 interactors.
STRINGi3702.AT1G02970.1.

Structurei

3D structure databases

ProteinModelPortaliQ8L4H0.
SMRiQ8L4H0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini249 – 495Protein kinasePROSITE-ProRule annotationAdd BLAST247

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0601. Eukaryota.
ENOG410XS1M. LUCA.
HOGENOMiHOG000083236.
InParanoidiQ8L4H0.
KOiK06632.
OMAiNTVKSKR.
OrthoDBiEOG09360DKT.
PhylomeDBiQ8L4H0.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8L4H0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEKNGRTLL AKRKTQGTIK TRASKKIRKM EGTLERHSLL QFGQLSKISF
60 70 80 90 100
ENRPSSNVAS SAFQGLLDSD SSELRNQLGS ADSDANCGEK DFILSQDFFC
110 120 130 140 150
TPDYITPDNQ NLMSGLDISK DHSPCPRSPV KLNTVKSKRC RQESFTGNHS
160 170 180 190 200
NSTWSSKHRV DEQENDDIDT DEVMGDKLQA NQTERTGYVS QAAVALRCRA
210 220 230 240 250
MPPPCLKNPY VLNQSETATD PFGHQRSKCA SFLPVSTSGD GLSRYLTDFH
260 270 280 290 300
EIRQIGAGHF SRVFKVLKRM DGCLYAVKHS TRKLYLDSER RKAMMEVQAL
310 320 330 340 350
AALGFHENIV GYYSSWFENE QLYIQLELCD HSLSALPKKS SLKVSEREIL
360 370 380 390 400
VIMHQIAKAL HFVHEKGIAH LDVKPDNIYI KNGVCKLGDF GCATRLDKSL
410 420 430 440 450
PVEEGDARYM PQEILNEDYE HLDKVDIFSL GVTVYELIKG SPLTESRNQS
460 470 480 490 500
LNIKEGKLPL LPGHSLQLQQ LLKTMMDRDP KRRPSARELL DHPMFDRIRG
Length:500
Mass (Da):56,530
Last modified:October 1, 2002 - v1
Checksum:i7E7C32697B9A9E44
GO

Sequence cautioni

The sequence AAF02869 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB077385 mRNA. Translation: BAC11716.1.
AJ439593 mRNA. Translation: CAD28679.1.
AC009525 Genomic DNA. Translation: AAF02869.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27508.1.
PIRiC86160.
RefSeqiNP_171796.1. NM_100178.3.
UniGeneiAt.42570.

Genome annotation databases

EnsemblPlantsiAT1G02970.1; AT1G02970.1; AT1G02970.
GeneIDi839453.
GrameneiAT1G02970.1; AT1G02970.1; AT1G02970.
KEGGiath:AT1G02970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB077385 mRNA. Translation: BAC11716.1.
AJ439593 mRNA. Translation: CAD28679.1.
AC009525 Genomic DNA. Translation: AAF02869.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27508.1.
PIRiC86160.
RefSeqiNP_171796.1. NM_100178.3.
UniGeneiAt.42570.

3D structure databases

ProteinModelPortaliQ8L4H0.
SMRiQ8L4H0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24688. 8 interactors.
IntActiQ8L4H0. 8 interactors.
STRINGi3702.AT1G02970.1.

Proteomic databases

PaxDbiQ8L4H0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G02970.1; AT1G02970.1; AT1G02970.
GeneIDi839453.
GrameneiAT1G02970.1; AT1G02970.1; AT1G02970.
KEGGiath:AT1G02970.

Organism-specific databases

TAIRiAT1G02970.

Phylogenomic databases

eggNOGiKOG0601. Eukaryota.
ENOG410XS1M. LUCA.
HOGENOMiHOG000083236.
InParanoidiQ8L4H0.
KOiK06632.
OMAiNTVKSKR.
OrthoDBiEOG09360DKT.
PhylomeDBiQ8L4H0.

Enzyme and pathway databases

ReactomeiR-ATH-69656. Cyclin A:Cdk2-associated events at S phase entry.

Miscellaneous databases

PROiQ8L4H0.

Gene expression databases

GenevisibleiQ8L4H0. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWEE1_ARATH
AccessioniPrimary (citable) accession number: Q8L4H0
Secondary accession number(s): Q9SRY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.