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Q8L4H0 (WEE1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Wee1-like protein kinase

EC=2.7.10.2
Alternative name(s):
Wee1-At
Gene names
Name:WEE1
Ordered Locus Names:At1g02970
ORF Names:F22D16.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell cycle regulatory kinase that is not rate-limiting for cycle progression under normal growth conditions. Transcriptionally activated upon DNA stress or damage in an ATR- or ATM-dependent manner. Once activated, inhibits plant growth by arresting dividing cells in the G2 phase before proceeding into mitosis. Down-regulates CDKA-1 and CDKD-2 by tyrosine phosphorylation. May target principally CDKA-1. Ref.1 Ref.2 Ref.5

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Interacts with CDKA-1, but not with CDKB1-1. Ref.5

Subcellular location

Nucleus Ref.2.

Tissue specificity

Expressed in shoot apex, vasculatures tissues of roots and leaves, and developing flowers. Ref.1 Ref.5

Induction

By replication blocking agents (hydroxyurea and aphidicolin). Ref.5

Disruption phenotype

Plants show no obvious cell division or endoreduplication phenotype when grown under nonstress conditions, but are hypersensitive to agents that impair DNA replication. Ref.5

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAF02869.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Wee1-like protein kinase
PRO_0000295672

Regions

Domain249 – 495247Protein kinase
Nucleotide binding255 – 2639ATP By similarity

Sites

Active site3721Proton acceptor By similarity
Metal binding3771Magnesium; via carbonyl oxygen By similarity
Metal binding3891Magnesium; via carbonyl oxygen By similarity
Binding site2781ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8L4H0 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 7E7C32697B9A9E44

FASTA50056,530
        10         20         30         40         50         60 
MFEKNGRTLL AKRKTQGTIK TRASKKIRKM EGTLERHSLL QFGQLSKISF ENRPSSNVAS 

        70         80         90        100        110        120 
SAFQGLLDSD SSELRNQLGS ADSDANCGEK DFILSQDFFC TPDYITPDNQ NLMSGLDISK 

       130        140        150        160        170        180 
DHSPCPRSPV KLNTVKSKRC RQESFTGNHS NSTWSSKHRV DEQENDDIDT DEVMGDKLQA 

       190        200        210        220        230        240 
NQTERTGYVS QAAVALRCRA MPPPCLKNPY VLNQSETATD PFGHQRSKCA SFLPVSTSGD 

       250        260        270        280        290        300 
GLSRYLTDFH EIRQIGAGHF SRVFKVLKRM DGCLYAVKHS TRKLYLDSER RKAMMEVQAL 

       310        320        330        340        350        360 
AALGFHENIV GYYSSWFENE QLYIQLELCD HSLSALPKKS SLKVSEREIL VIMHQIAKAL 

       370        380        390        400        410        420 
HFVHEKGIAH LDVKPDNIYI KNGVCKLGDF GCATRLDKSL PVEEGDARYM PQEILNEDYE 

       430        440        450        460        470        480 
HLDKVDIFSL GVTVYELIKG SPLTESRNQS LNIKEGKLPL LPGHSLQLQQ LLKTMMDRDP 

       490        500 
KRRPSARELL DHPMFDRIRG 

« Hide

References

« Hide 'large scale' references
[1]"A WEE1 homologue from Arabidopsis thaliana."
Sorrell D.A., Marchbank A., McMahon K., Dickinson J.R., Rogers H.J., Francis D.
Planta 215:518-522(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Diverse phosphoregulatory mechanisms controlling cyclin-dependent kinase-activating kinases in Arabidopsis."
Shimotohno A., Ohno R., Bisova K., Sakaguchi N., Huang J., Koncz C., Uchimiya H., Umeda M.
Plant J. 47:701-710(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Arabidopsis WEE1 kinase controls cell cycle arrest in response to activation of the DNA integrity checkpoint."
de Schutter K., Joubes J., Cools T., Verkest A., Corellou F., Babiychuk E., van der Schueren E., Beeckman T., Kushnir S., Inze D., de Veylder L.
Plant Cell 19:211-225(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION, INTERACTION WITH CDKA-1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB077385 mRNA. Translation: BAC11716.1.
AJ439593 mRNA. Translation: CAD28679.1.
AC009525 Genomic DNA. Translation: AAF02869.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27508.1.
PIRC86160.
RefSeqNP_171796.1. NM_100178.2.
UniGeneAt.42570.

3D structure databases

ProteinModelPortalQ8L4H0.
SMRQ8L4H0. Positions 215-495.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid24688. 8 interactions.
IntActQ8L4H0. 8 interactions.
STRING3702.AT1G02970.1-P.

Proteomic databases

PRIDEQ8L4H0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G02970.1; AT1G02970.1; AT1G02970.
GeneID839453.
KEGGath:AT1G02970.

Organism-specific databases

GeneFarm3422.
TAIRAT1G02970.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000083236.
InParanoidQ8L4H0.
KOK06632.
OMARYRTDFH.
PhylomeDBQ8L4H0.

Enzyme and pathway databases

BioCycARA:AT1G02970-MONOMER.

Gene expression databases

GenevestigatorQ8L4H0.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameWEE1_ARATH
AccessionPrimary (citable) accession number: Q8L4H0
Secondary accession number(s): Q9SRY9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names