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Q8L4H0

- WEE1_ARATH

UniProt

Q8L4H0 - WEE1_ARATH

Protein

Wee1-like protein kinase

Gene

WEE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Cell cycle regulatory kinase that is not rate-limiting for cycle progression under normal growth conditions. Transcriptionally activated upon DNA stress or damage in an ATR- or ATM-dependent manner. Once activated, inhibits plant growth by arresting dividing cells in the G2 phase before proceeding into mitosis. Down-regulates CDKA-1 and CDKD-2 by tyrosine phosphorylation. May target principally CDKA-1.3 Publications

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei278 – 2781ATPPROSITE-ProRule annotation
    Active sitei372 – 3721Proton acceptorPROSITE-ProRule annotation
    Metal bindingi377 – 3771Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi389 – 3891Magnesium; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi255 – 2639ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    4. protein binding Source: TAIR
    5. protein kinase activity Source: TAIR
    6. protein serine/threonine kinase activity Source: InterPro

    GO - Biological processi

    1. cell cycle arrest Source: TAIR
    2. DNA replication checkpoint Source: TAIR
    3. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G02970-MONOMER.
    ReactomeiREACT_203219. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Wee1-like protein kinase (EC:2.7.10.2)
    Alternative name(s):
    Wee1-At
    Gene namesi
    Name:WEE1
    Ordered Locus Names:At1g02970
    ORF Names:F22D16.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G02970.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Plants show no obvious cell division or endoreduplication phenotype when grown under nonstress conditions, but are hypersensitive to agents that impair DNA replication.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 500500Wee1-like protein kinasePRO_0000295672Add
    BLAST

    Proteomic databases

    PRIDEiQ8L4H0.

    Expressioni

    Tissue specificityi

    Expressed in shoot apex, vasculatures tissues of roots and leaves, and developing flowers.2 Publications

    Inductioni

    By replication blocking agents (hydroxyurea and aphidicolin).1 Publication

    Gene expression databases

    GenevestigatoriQ8L4H0.

    Interactioni

    Subunit structurei

    Interacts with CDKA-1, but not with CDKB1-1.1 Publication

    Protein-protein interaction databases

    BioGridi24688. 8 interactions.
    IntActiQ8L4H0. 8 interactions.
    STRINGi3702.AT1G02970.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8L4H0.
    SMRiQ8L4H0. Positions 215-495.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini249 – 495247Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000083236.
    InParanoidiQ8L4H0.
    KOiK06632.
    OMAiRYRTDFH.
    PhylomeDBiQ8L4H0.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8L4H0-1 [UniParc]FASTAAdd to Basket

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    MFEKNGRTLL AKRKTQGTIK TRASKKIRKM EGTLERHSLL QFGQLSKISF    50
    ENRPSSNVAS SAFQGLLDSD SSELRNQLGS ADSDANCGEK DFILSQDFFC 100
    TPDYITPDNQ NLMSGLDISK DHSPCPRSPV KLNTVKSKRC RQESFTGNHS 150
    NSTWSSKHRV DEQENDDIDT DEVMGDKLQA NQTERTGYVS QAAVALRCRA 200
    MPPPCLKNPY VLNQSETATD PFGHQRSKCA SFLPVSTSGD GLSRYLTDFH 250
    EIRQIGAGHF SRVFKVLKRM DGCLYAVKHS TRKLYLDSER RKAMMEVQAL 300
    AALGFHENIV GYYSSWFENE QLYIQLELCD HSLSALPKKS SLKVSEREIL 350
    VIMHQIAKAL HFVHEKGIAH LDVKPDNIYI KNGVCKLGDF GCATRLDKSL 400
    PVEEGDARYM PQEILNEDYE HLDKVDIFSL GVTVYELIKG SPLTESRNQS 450
    LNIKEGKLPL LPGHSLQLQQ LLKTMMDRDP KRRPSARELL DHPMFDRIRG 500
    Length:500
    Mass (Da):56,530
    Last modified:October 1, 2002 - v1
    Checksum:i7E7C32697B9A9E44
    GO

    Sequence cautioni

    The sequence AAF02869.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB077385 mRNA. Translation: BAC11716.1.
    AJ439593 mRNA. Translation: CAD28679.1.
    AC009525 Genomic DNA. Translation: AAF02869.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE27508.1.
    PIRiC86160.
    RefSeqiNP_171796.1. NM_100178.2.
    UniGeneiAt.42570.

    Genome annotation databases

    EnsemblPlantsiAT1G02970.1; AT1G02970.1; AT1G02970.
    GeneIDi839453.
    KEGGiath:AT1G02970.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB077385 mRNA. Translation: BAC11716.1 .
    AJ439593 mRNA. Translation: CAD28679.1 .
    AC009525 Genomic DNA. Translation: AAF02869.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE27508.1 .
    PIRi C86160.
    RefSeqi NP_171796.1. NM_100178.2.
    UniGenei At.42570.

    3D structure databases

    ProteinModelPortali Q8L4H0.
    SMRi Q8L4H0. Positions 215-495.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 24688. 8 interactions.
    IntActi Q8L4H0. 8 interactions.
    STRINGi 3702.AT1G02970.1-P.

    Proteomic databases

    PRIDEi Q8L4H0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G02970.1 ; AT1G02970.1 ; AT1G02970 .
    GeneIDi 839453.
    KEGGi ath:AT1G02970.

    Organism-specific databases

    GeneFarmi 3422.
    TAIRi AT1G02970.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000083236.
    InParanoidi Q8L4H0.
    KOi K06632.
    OMAi RYRTDFH.
    PhylomeDBi Q8L4H0.

    Enzyme and pathway databases

    BioCyci ARA:AT1G02970-MONOMER.
    Reactomei REACT_203219. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

    Gene expression databases

    Genevestigatori Q8L4H0.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Diverse phosphoregulatory mechanisms controlling cyclin-dependent kinase-activating kinases in Arabidopsis."
      Shimotohno A., Ohno R., Bisova K., Sakaguchi N., Huang J., Koncz C., Uchimiya H., Umeda M.
      Plant J. 47:701-710(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
      Strain: cv. Columbia.
    3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Arabidopsis WEE1 kinase controls cell cycle arrest in response to activation of the DNA integrity checkpoint."
      de Schutter K., Joubes J., Cools T., Verkest A., Corellou F., Babiychuk E., van der Schueren E., Beeckman T., Kushnir S., Inze D., de Veylder L.
      Plant Cell 19:211-225(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION, INTERACTION WITH CDKA-1.

    Entry informationi

    Entry nameiWEE1_ARATH
    AccessioniPrimary (citable) accession number: Q8L4H0
    Secondary accession number(s): Q9SRY9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2007
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3